Objective: To investigate the relationship between serum albumin level and severity of coronary heart disease (CHD). Methods: A total of 237 patients admitted in our hospital from 2008 to 2009 and undergoing coronary angiography were enrolled. CHD severity was evaluated according to clinical types, number of diseased coronary vessels and coronary artery score respectively, and patients were divided into non-CHD group (n=84), angina pectoris group (n=92) and myocardial infarction group (n=61). Serum total protein, albumin and globulin were measured in all patients on second day after admission. Relationship among indexes of CHD severity and serum proteins were analyzed. Results: Compared with non-CHD group, there was significant decrease in albumin level [(40.22±3.56) g/L vs. (39.81±3.56) g/L vs. (38.68±3.43) g/L, P<0.05] in angina pectoris group and myocardial infarction group. Spearman correlation analysis indicated that serum albumin level was significant negatively correlated with clinical types (r=-0.18, P=0.005), number of diseased coronary vessels (r=-0.195, P=0.003) and coronary artery score (r=-0.188, P=0.004). Serum total protein, globulin and ratio of albumin/globulin were not significantly correlated with clinical types, number of diseased coronary vessels and coronary artery score (P>0.05). Conclusion: Serum albumin level is negatively correlated with severity of coronary heart disease. Low serum albumin may be a risk factor for coronary heart disease.

The concept and important role of associative memory neural network are introduced.The development of associative memory neural network is also summarized along with other neural networks such as the Hopfield neural network,the neural network composed of FitzHugh-Nagumo neuron model,the neural network composed of Hodgkin-Huxley neuron model.

ObjectiveTo investigate the influence on negative emotion of ICU patient's relatives by comprehensive needs intervention.Methods 100 ICU patient's relatives were randomly divided into intervention group and control group,50 cases per group.Patients in intervention group were given comprehensive needs interventions for two weeks,then were tested by self-rating anxiety scale(SAS),self-rating depression scale(SDS) and symptom checklist 90 (SCL-90),and their negative emotion were compared.ResultsExcept for interpersonal relationship factor (P＞ 0.05 ),the other SCL-90 factorial scores of ICU patient's relative were all significantly higher than the national norm before intervention(P ＜ 0.01 ～0.05 ).Anxiety and depression rates in two groups were 64％ and 53％ respectively,SDS standard scores were 49.62 ± 9.18 and 49.70 ± 9.73,SAS scores were 40.08± 11.93 and 40.04 ± 11.76,and they were all significantly higher than normal norm.After intervention,SAS,SDS standard scores and SCL-90 factors of intervention group were significantly decreased,and were superior than control group,and there were statistical difference (P＜ 0.01 ～ 0.05 ).ConclusionThere are so much psychological diseases with the relatives of ICU patients,especially anxiety and depression.

The cell-free plasma DNA of the 132 critically ill patients admitted to the emergency intensive care unit ( EICU) was measured by real-time quantitative polymerase chain reaction assay for the humanβ-globin gene. Ac-cording to the prognosis, the patients were divided into survival group (95 cases) and death group (37 cases). Blood samples were collected after EICU admission, 24 hours after admission, 48 hours after admission, and 1 week after admission. 30 age-mached controls were collected from healthy volunteers healthy care centre. Com-pared with control group, at each time point, cell-free plasma DNA concentration was significantly higher in EICU patients (P<0. 05). Cell-free plasma DNA concentrations were higher in hospital non-survivors than in survivors, of which plasma DNA concentration of 24 hours after admission and 48 hours after admission was statistically signifi-cant ( P<0 . 05 ) . The area under the ROC curves ( AUC ) for plasma DNA was bigger than the lactate and A-PACHE IIscore, the maximum of AUC was 0. 847(95% CI 0.648~1.000) at the point of 48 hours after admis-sion.

Objective To investigate the effect of vitamin D (VD) on macrophage to phagocytize Staphylococcus aureus (SA). Method Macrophoge cell lines RAW264.7 were allocated into 3 groups:control group(C),bacterium group(B),active vitamin D+ bacterium group (VD+B). Cells in the VD+B group were incubated with 10-8mol/L active vitamin D for 24h,then cells in the VD+B group and the B group were cultured with SA for 1h,and phagocytosis rate,mitochondrial membrane potential,[Ca～(2+)]i,reactive oxygen species were determined by flow cytometry (FCM). Results The phagocytizing activity of macrophage in VD+B group was significantly higher than that in B group 1h after infection,At the same time,the mitochondrial member potential and [Ca～(2+)]i of macrophage in VD+B group were distinctly lower than that in B group; but reactive oxygen species of macrophage in the VD+B group was insignificantly different from B group. Conclusion Vitamin D can reinforce the phagocytizing activity of macrophage and inhibit the apoptosis of macrophage after phagocytize Staphylococrcus aureus.

Objective　To observe the curative effect of adult ischemic necrosis of femoral head by intramedullary decompression and arterial pulse pressor infusion.Methods　82 patients with ischemic necrosis of femoral head were treated in the combined treatment of percutaneous intramedully decompression and pulse pressor five medicine combined infusion in target vessels using ZC-Ⅰ-discome.Results　By follow-up for 12～48 months,the painful in hip joint was alleviated and joint movement was also improved.The regenerative vessels and bone in femoral head and neek were obvious.Conclusion　This therapcutic methods is safe and superior to single interventional medicine treatment for ischemic necrosis of femoral head.

Objective To discuss the application of 18F-FDG PET/CT in epileptogenic zone localization.Methods Tirty-two patients with epilepsies were studied with interictal 18F-FDG PET/CT cerebral imaging,and all of them had scalp EEG,CT or MRI results for comparision.Results The sensitivity of 18F-FDG PET/CT was 90.6%,27 patients(84.5%) showed focal hypometabolism,2 patients(6.3%) showed focal hypermetabolism and rest 3 patients(9.4%) had negative result.The sensitivity of EEG was 65.6%(21/32),the sensitivity of CT and MRI was 31.0%(10/32).Conclusion 18F-FDG PET/CT was a sensitive and accurate image modality in the localization of epileptic foci.

Objective:To check RNA binding protein QKI-5's expression level in breast cancer cells and inhibiting effect on cancer cell proliferation.Methods:QKI-5's expression level was checked in different breast cancer cells by Western blotting,cancer cells of overexpressed QKI-5 gene could be stabilized by slow virus infection construction,MTT and FCM were used to check cell period to express QKI-5's influence on cell proliferation and period.Results:MCF-7 cells have relatively low QKI-5 expression level in three breast cancer cells,MTT experiment result has obvious reduced influence on QKI-5's expression of MCF-7 cell proliferation P＜0.05,mean-while,cell period inspection shows that overexpressed QKI-5's MCF-7 cells have obvious G1 retardant,cells in S and G2/M periods are reduced.Conclusion:High expression of QKI-5'in breast cancer could cause slow cell proliferation by inhibiting cancer cell period,hence causing limited tumor growth.

Objective Diallyl disulfide ( DADS) has achieved remarkable effects in treatment and research of diverserfied cancers.The article was to explore the effects and the mechanism of DADS on the xenograft growth of human small cell lung cancer ( SCLC) cells in nude mice . Methods A total of 25 nude mice were selected to establish xenograft model of NCI-H446 human SCLC cells.The nude mice bearing with SCLC H446 were divided into 5 groups by random selection:positive control group(DDP 66 mg/kg), negative control group(physiological saline), 20 mg/kg DADS group, 60 mg/kg DADS group and 180 mg/kg DADS group, which is 40.6%, 53.1%and 66.4%, respectively.The growth of xenograft tumor in mice was observed after being treated with differ-ent concentrations of DADS .The morphological changes of the tumors were examined under light microscopy .Phase distribution and apoptosis of xenograft cells were analyzed by flow cytometry ( FCM) . Results The growth of xenograft tumor were inhibited signifi-cantly by DADS, resulting in decreased cell density and cellular atypia .Moreover, xenograft cell cycle was blocked in G 2/M and cell apoptosis rate was enhanced . Conclusion DADS can significantly inhibit the growth of NCI-H446 cells and lead to apoptosis .