With the rapid increase of educational resources, how to search for necessary educational resource quickly is one of most important issues. Educational resources have the characters of distribution and heterogeneity, which are the same as the characters of Grid resources. Therefore, the technology of Grid resources search was adopted to implement the educational resources search. Motivated by the insufficiency of currently resources search methods based on metadata, a method of extracting semantic relations between words constituting metadata is proposed. We mainly focus on acquiring synonymy, hyponymy, hypernymy and parataxis relations. In our schema, we extract texts related to metadata that will be expanded from text spatial through text extraction templates. Next, metadata will be obtained through metadata extraction templates. Finally, we compute semantic similarity to eliminate false relations and construct a semantic expansion knowledge base. The proposed method in this paper has been applied on the education grid.

Objective To study the apoptosis of human leukemia K562 cells induced by snake venom of Agkistrodon Halys Pallas in Zhejiang Province.Methods IC_ 50 value and cytotoxity of K562 cell were detected by MTT method.Apoptotic cells were dyed by Hoechest 33258.Sub-G1 peak and cell cycle were detected by FCM.Protein expression of Bcl-2 gene was detected by FCM and western-blot method.Results The snake venom of Agkistrodon Halys Pallas in Zhejiang Province inhibited the growth of K562 cells,which appeared dose-dependent.The snake venom induced apoptosis of K562 cells.Meanwhile,protein expression of Bcl-2 was down-regulated.Conclusion Snake venom of Agkistrodon Halys Pallas in Zhejiang could induce apoptosis of human leukemia K562 cells.The mechanism may be related to down-regulation of Bcl-2 gene expression.

Objective: Aiming at lots of vulnerabilities in the Wired Equivalent Privacy (WEP) which threats the authentication and confidentiality in wireless communication, a new kind of mutual authentication and privacy mechanism named MWEP (Modified WEP) is proposed. Methods: MWEP is based on pseudo random number generator (PRNG) and asymmetric cryptograph approach, it generates a unique session-key like "One-Time Password" for each data frame transmission between any two mobile stations. Results: Using this session-key to encrypt the transmission data, not only can it avoid replay attack, but also provide a good secure virtual channel for the sender and receiver. MWEP can be incorporated into IEEE 802. 11. Conclusion: It shows that the proposed mechanism is effective and practical after comparison with WEP and simulation.

History of abdominal surgery was the contradiction of reoperation with laparoscopy. With the development of laparoscopic technique, this contradiction now has been challenged and reoperative minimally invisave surgery is growing. The objective of this article is to state the applications of laparoscopic technique in gastrointestinal reoperative surgery.

Objective To establish the standard for the quality control of Yubo Shaoshang Spray.Methods The identification of Rhizoma Polygoni Cuspidati and Cortex Phellodendri in Yubo Shaoshang Spray were carried out by TLC.The content of emodin in the preparation was determined by HPLC.Chromatographic column was adopted,methanol-0.1 %H3PO4 (85 ∶15) was used as the mobile phase,flow rate 0.80 mL/min, column temperature at 40℃and detection wavelength at 254 nm.Results The TLC spots were highly clear without the interference of negative control and were reproductive.The calibration curve for emodin was linear(r=0.9993) in the range of 0.1～0.5 ?g and the mean recovery rate was 99.34 %and RSD=1.33 %(n=5).Conclusion This method is simple and accurate for the quality control of Yubo Shaoshang Spray.

Objective To investigate the changes of enteric nervous system in gastrointestinal motility disorder in rats with severe acute pancreatitis (SAP) as well as the influence of Chaishao Chengqi Decoction on neuronal nitric oxide synthase neuron (nNOS) neuron expression in myenteric plexus of rat intestinal tract. Methods SD rats were randomly divided into 3 groups (10 for each): sham operation group, model group and Chaishao Chengqi Decoction (CSCQD) group. SAP model was established in taurocholate into the junction of the common bile duct and pancreatic duct. Model group was administered with normal saline and CSCQD group was administered with Chaishao Chengqi Decoction. The gastrointestinal transmission time was observed. Pathological changes of the pancreas were examined with HE staining under light microscope. The neuronal nitric oxide synthase neurons in the small intestines and colonic myenteric plexus were detected by the dual immunofluorescent staining. Results Compared with that in model group, transmission index was increased in CSCQD group (P<0.05). The pathological lesion of the pancreas in CSCQD group was significantly lessened (P<0.05). The percentage of NOS-immunoreactive neurons was significantly decreased (P<0.05). Conclusion By reversing the plasticity of NOS-immunoreactive neurons, Chaishao Chengqi Decoction improves the gastrointestinal motility in rats with SAP and reduces the pathological damages of the pancreas.

ObjectiveTo observe the therapeutic effects of calcium dobesilate combined with irbesartan in treating early diabetic nephropathy ( DN ).Methods65 patients with early DN were randomly divided into two groups,experiment group( irbesartan plus calcium dobesilate,n =34) and control group( irbesartan,n =31 ).After 24 week treatment,the urinary albumin excretion rate(UAER),fasting blood glucose ( FBG ),postprandial blood glucose (PBG) and glycosylated hemoglobin(HbA1c) were detected and compared between two groups before and after treat ment.ResultsUAER after treatment was significantly reduced in both 2 groups(t =8.074,12.89,all P＜0.01),and the decrement in the experiment group was more obvious than that in the control group with statistical significance ( t =3.965,P ＜ 0.05).ConclusionCalcium dobesilate combined with irbesartan has good effects in the treatment of early DN.

The signification and status quo of value engineering are introduced. Its application and significance in clinical medical engineering especially in the quality-control of medical instrument are also discussed.

Objective To investigate and confirm the value of minimal invasion of separation between specific subcutancous layers of rats.Methods Thirty-two male SD rats were randomized into four groups,group 1:normal incision plus separation beneath superficial fascia; group 2:normal incision plus subdermal separation; group 3:normal incision plus extended separation beneath superficial fascia ; group 4:extended incision plus separation beneath superficial fascia.Blood sampling was taken preopeatively,2 h,12 h,24 h and 48 h postoperatively.The increase of serum IL-6 and neutrophil elastase levels were compared between different groups.Results Compared with each other,the increase of serum IL-6 and NE levels in group 1 vs 3 and group 2 vs 4 was not different significantly (P value was 0.074,0.096 and 0.747,0.897,respectively).However,the increase of serum levels in group 1 vs 2,1 vs 4,3 vs 2 and 3 vs 4 was different significantly (P ＜ 0.01).Conclusion Operation of separating beneath superficial fascia plus small skin incision in rats conduces to relatively minimal invasion.

AIM: To study the effect and mechanism of chlorophyllin (CHL) inhibiting HT29 cells. METHODS: IC 50 value and growth curve of HT29 cells were detected with MTT method. Apoptosis was detected with Wright-Giemsa staining, FCM and DNA electrophoresis. Telomerase was detected by PCR-ELISA, and protein and mRNA expression of COX-2 gene were detected through RT-PCR and Western blot. RESULTS: CHL inhibited the growth of HT29 in a dose-dependent manner. CHL blocked HT29 cells in G 1 phase but did not induce apoptosis. Different concentration of CHL inhibits the expression of telomerase and COX-2 in HT29 cells. CONCLUSION: CHL inhibits the growth of HT29 cells by inhibiting the expression of telomerase and COX-2 and blocking cells in G 1 phase. [