The function of the retina is sensitive to oxygen tension, and any changes in oxygen perfusion associated with retinal vessel diseases, such as diabetic retinopathy could affect inner retinal oxygen responses (ΔPO_2). However, the application of currently available techniques in measurements of the retinal oxygenation is limited. Recently, with the development of MRI, it is possible to detect ΔPO_2 noninvasively, and the effects are satisfactory. The application of MR based measurements of ΔPO_2 following the shift from breathing room air were reviewed in this article.

Objective To compare the effect of different sources extracted scutellaria (Hebei, Shanxi and Dalian) on the death-protection and inhibition of FM1 infected mice. Method As animal model, FM1 infected mice was treated an hour after infection. Indexes including mortality, average survival time, lung index were observed to evaluate the effect of different sources extracted scutellaria on anti-influenza virus. Result Different sources extracted scutellaria reduced mortality (P

Objective To evaluate the clinical value of serum procalcitonin (PCT) in the diagnosis and differentiation of bacterial infections in the critically ill patients. Methods A total of 53 critical patients were divided into bacterial infection group and non-bacterial infection group. Serum PCT was measured by immunoluminometric assay. Results PCT of bacterial infection group was significantly higher than that in non-bacterial infection group (?~2=0.05, P

0.05).No significant difference on mortality was found between infection disease and non-infection disease.The mortality of patients with platelet count less than or equal to 50?109/L was higher than those with platelet count greater than 50?109/L(P

Objective To realize the hygienic state of drinking water in the rural areas in Yunnan Province,the scientific evidences would be provided for drawing the policies about improving the drinking water quality in the rural areas.Methods From August to October in 2006,30 counties were randomly chosen among the monitoring counties,in each county at least 10 sampling points were chosen randomly.The questionnaires were employed and the water samples were collected,the quality of drinking water was tested with the related standard methods.Results 8.94 million people were involved in the survey,48.60% of them used surface water as the source of water.52.18% of people were serviced by the central water supply.The eligible rate in 301 water samples in this survey was 36.88%,those of the samples of surface and underground water were 37.60% and 36.36% respectively.The eligible rate of samples from central water supply was 41.85%,higher than that from non-central water supply(P

Objective To investigate the gastric mucosal characteristics of Helicobacter pylori(Hp)-associated gastritis under high-resolution magnifying endoscopy. Methods Fujinon EG 485 ZH 850k CCD high-resolution electronic magnifying endoscopy was performed. Fifty patients were inspected by regular endoscopy with high-resolution magnifying function. Particular inspection was given on the collecting venules, the pit patterns of the gastric antrum and gastric body. Four pieces of biopsy specimen were taken, 1 each in antrum and body for RUT and the other two for pathologic examination as well as W-S stain. Both of the W-S stain and RUT being positive were confirmed as Hp infection. The Hp-positive patients received the triple therapy. Endoscopy with biopsies was repeated 4 weeks after the course of eradication treatment ended. The results were assessed by SPSS 8. 0 statistical package. Results Twenty-six patients were Hp-positive and 24 patients Hp-negative. In comparing with the results of W-S stain and RUT , the high-resolution magnifying endoscopy features and regular endoscopy findings had different outcomes. The sensitivity was 69. 23% Vs 34. 62% ; the accuracy 78.00% Vs 58. 00% respectively; the positive likelihood ratio and the negative likelihood ratio were 5. 54 Vs 2. 08 and 0. 35 Vs 0. 78 respectively. The Kappa value in high-resolution magnifying endoscopy was 0.563 whereas in regular endoscopy was 0. 176. Conclusions The advantage of high-resolution magnifying endoscopy relies on its ability to observe the fine gastric mucosal patterns. The gastric mucosal features of Hp-associated gastritis can be described as followings: collecting venules are irregular or disappeared; the gastric pits are enlarged or reddened, the sulciolar are deepened. Compared with regular endoscopy, the high-resolution magnifying endoscopy is better in sensitivity, accuracy and likelihood ratio. A high degree of interobserver agreement was obtained with the Kappa value. The pathologic findings get better soon after the eradication therapy, while the endoscopic changes are not prominent.

Objective To investigate the correlation between platelet-related parameters and the severity as well as prognosis of septic patients.Methods A total of 91 patients with sepsis were included in this study, and the platelet-related parameters were detected in all patients within 24 hours and 72 hours after admission to hospital, respectively.Clinical information of each patient was recorded including age, gender and underlying diseases, APACHE Ⅱ score at admission and the incidence of the consequent multiple organ dysfunction syndrome (MODS) and 28-day mortality.The differences in platelet-related parameters between non-severe sepsis group and severe sepsis group were compared, the correlation between plateletrelated parameters and the prognosis was studied by using rank method and the reliability of platelet-related parameters to predict the prognosis was estimated by using receiver operating characteristic curve (ROC).Results The differences in results of platelet-related parameters between non-severe sepsis group and severe sepsis group within 24 hours and 72 hours after admission were as follows : platelet count : (166.34 ± 58.27) ×109L-1vs.(198.57±65.82) ×109L-1, P=0.02and (138.85 ± 53.31) ×109L-1vs.(173.79 ± 67.48) × 109 L-1, P =0.00;the platelet distribution width (PDW) : (13.84 ± 2.46) ％ vs.(12.73±1.72)％, P=0.01 and (16.07 ±2.87)％ vs.(13.86 ±2.31)％, P=0.00;mean platelet volume (MPV) : (10.17 ±1.82) fl (femto-liter) vs.(9.32 ±1.34) fl, P=0.01 and (11.49 ± 1.53)fl vs.(10.37 ± 1.24) fl, P =0.00, respectively.The value of the PDW showed positive correlation with the prognosis (dead) (r=0.51, P＜0.05), and the MPV did likewise (r=0.53, P＜0.05, while the platelet count negatively correlated (r =-0.61, P ＜ 0.05), These characteristics were more obvious at 72 hours after admission (rPDW =0.68, rPv =0.67, rPLT =-0.71, P ＜0.01).The areas under the ROC curve 72 hours after admission as follows: PLT count0.95, PDW0.93 and MPV0.93, respectively, which were higher than those of PLT count (0.88), PDW (0.82) and MPV (0.83) within 24 hours after admission.The sensitivity and specificity of platelet count 72 hours after admission predicting the prognosis of death were 91.24％ and 80.35％, respectively.Conclusions The changes of platelet-related parameters in septic patients are related to the severity of the disease, and platelet count at 72 hours after admission may play an important role in prognosis of disease.

OBJECTIVE To study the isolation status and antimicrobial resistance of nonfermenting Gram-negative bacilli collected from intensive care unit(ICU) of our hospital so as to instruct the rational clinical application of antibiotics.METHODS The antimicrobial resistance of nonfermenting Gram-negative bacilli isolates collected from patients in ICU from Jan 2003 to Dec 2007 was analyzed.Antimicrobial susceptibility of clinical isolates were tested by Kirby-Bauer method.RESULTS Total 384 nonfermenting Gram-negative bacilli isolates were collected in 5 years.The most common species were Acinetobacter baumannii(219),Pseudomonas aeruginosa(117) and Stenotrophomonas maltophilla(36).The antimicrobial resistance rate of nonfermenting Gram-negative bacterial to most antibiotics were much higher.The antimicrobial resistance rate of Acinetobacter spp to imipenem,cefoperazone/sulbactam and piperacillin/tazobactam was 3.7%,28.3% and 42.9%.But the resistance rate of Acinetobacter spp to imipenem was increased in recent 2 years(58.0%).The antimicrobial resistance rate of P.aeruginosa to cefoperazone/sulbactam was the lowest.That of imipenem-resistant P.aeruginosa to cefoperazone/sulbactam was 34.0%.S.maltophilla was relatively susceptible to ceftazidime,cefoperazone/sulbactam and piperacillin/tazobactam.CONCLUSIONS Nonfermenters Gram-negative bacilli are the important pathogens in ICU.Surveillance of their prevalence and drug resistance may provide evidences for rational antibiotic choices.

Objective To investigate the occurrence of thyroid disorders in septic patients and the relationship between thyroid hormone level and the change of left ventricular function.Methods A total of 63 septic patients,43 male and 20 female,aged 50 to 88 years with mean 63.3 ± 18.5,were included in the present study.The thyroid hormone levels and C-reactive protein (CRP) levels were detected in all patients on the next day of admission.Clinical information of each patient was recorded including demographics and co-existed disease,APACHE Ⅱ score at admission and the incidence of shock and subsequent multiple organ dysfunction syndrome (MODS).At 24 hours,the second week and the third week after admission,a serial trans-thoracic echocardiography were performed in all patients to observe changes of left ventricular end diastolic dimension,left ventricular eject fraction and E/A (the ratio between maximal velocity of blood flow at mitral valve orifice in the early diastolic phase and maximal velocity of blood flow of atrium in systolic phase).Another 38 patients with ordinary infections,21 male and 17 female,with mean age 58.6 ± 16.8 (48-84 years) were selected as controls for comparison.The difference in thyroid function between sepsis group and control group was analyzed.The levels of serum thyroid hormone associated with the incidence of shock and MODS were studied among different types of the left ventricular structure and function in sepsis group.Results The levels of serum free tri-iodothyronine (FT3) and free tetra-iodothyronine (FT4) in sepsis group were significantly lower than those in control group (P ＜ 0.05),but there was no difference in level of thyroid stimulating hormone (TSH) between these two groups.The patients with sepsis had three types of the left ventricular dysfunction:diastolic dysfunction without the enlargement of left ventricular (type Ⅰ) in 31 patients,the enlargement of left ventricular with diastolic dysfunction (type Ⅱ) in 18 patients and the enlargement of left ventricular with both systolic and diastolic dysfunction (type Ⅲ) in 14 patients.Compared with the other two types,the type Ⅲ patients had significantly lower levels of FT3 and FT4 and higher incidences of shock and MODS (P ＜ 0.05).No significant difference in TSH level was found among the three types.There were no significantly differences in FT3 and FT4 between type Ⅰ and type Ⅱ,but the higher incidences of shock and MODS were found in type Ⅰ (P ＞ 0.05).Conclusions Most septic patients have thyroid function disorder.Serum thyroid hormone levels in early stage of sepsis have certain role in predicting changes in left ventricular structure and function in the late stage of disease.

Fascia,the initial response site for mechanical stimulation in manipulations,is also the target of the effect of manipulations.As the essence of manipulation is"force",how mechanical stimuli are transduced into neuroelectric and biochemical signals in the fascia and how physical and chemical signals of the fascia initiate the mechanical stimulation effect are the common key questions in the study of the principle of manipulation.The physical changes in the fascial connective tissue caused by the manipulation,such as the deformation and displacement of the fascial tissue,can act on the nerve end receptors in the fascial layer and generate neural electrical signals;they can also activate the mechanoreceptors on the fascial cell membrane and convert mechanical signals into chemical signals via mechanosensitive ion channel transduction,triggering a physicochemical coupling response in the fascial microenvironment and producing mechanical stimulation through neuro-endocrine-immune system pathways.The"mechanical force of manipulation"in the fascia is transmitted through the meridian to facilitate the body's perception and transmission of mechanical stimulation signals,indicating that the fascia is the"sensor"of coupled response to the physicochemical information of mechanical stimulation of manipulation.