Distribution of 〔14C〕 bimolane in mice was studied by means of whole body autoradiography. At 30min, and lh, 3h, 9 h, 24h after iv, the mice were briefly anesthetized with ether and frozen by immersing indry ice/acetone. The mice were processed for whole body auto-radiography by continual sections with LKB-2250 PMV big whole body automatic-manual frozen cutter. The exeriments showed that 〔24C〕 bimolane distributed rapidly into the various tissues after iv administrtion. The highest radioactivity was found in lung, kidney, gastrointestinal tract, then the tumor, skin etc, the lowest radioactivity was found in brain.

Resistance to adriamycin was developed in an orignally adriamycin- sensitive strain of Ehrlich ascites carcinoma (EAC) in vivo by treament with the adriamycin during 50 weekly passages of the tumor. The results showed that the growth of the resistant line was slower than that of the EAC, total ascites cell volume,total cell number and mean single-cell volume were significantly smaller of the resistant line than those of original sensitive line. Also, itwas found the homogerously staining region and double minute chromosome in the cells of resistant line. The adriamycin-resistant tumor produced cross resistance to vincristine.mitomycin C,and actinomycin D,while section resistance to VP-16. There was no change in the sensitivity of tumor to oridonin.

Objective: To evaluate the application of frontal flap in reconstructing the defect of nose, the clinical data of 12 cases were reported. Methods: Twelve patients with nasal skin cancers undertaking radical dissection of tumor accompanied frontal flap reconstructing one stage were reviewed retrospectively.Results: The overall following\|up rate was 100%, 10 of these patients curled slightly after reconstructing the nose, but influence of the nasal outline was little. Two cases were recurrent locally, one occurred 24 months after operation, the other occurred 38 months. Both of them received radiotherapy. Malformation arised after radiotherapy. The total successful rate of reconstruct nasal outlook was 83.33%, the 3\|year locally recurrent rate was 8.33%,5\|year survival rate ws 100%, 5\|year survival rate without tumor was 83.33%.Conclusion:The blood supply of frontal flap is abundance;healing up soon after reconstruction; the manipulation of surgery is simple and safe. It is worth applying this operation generally in clinical practice.

AIM: To investigate the antitumor and immunological activities of the polysaccharides obtained from rabdosia rubescens (RRP). METHODS: In vitro and in vivo tumor inhibitory experiments as well as MTT method were used. RESULTS: With increasing concentration, the RRP exerted direct cytotoxicity effect in vitro on the cell growth of EAC mice,and its inhibitory rate in vivo against sarcoma 180 reached 35% ( P

Objective To study fragile histidine triad gene(FHIT) gene mRNA expression in 60 samples of papillary thyroid carcinoma(PTC) and matched adjacent non-concerous tissues (NCE),and to explore the role of FHIT gene in the development in PTC.Methods Reverse transcription polymerase chain reaction(RT-PCR) was used to detect FHIT gene mRNA expression in 60 samples of PTC and matched NCE.Results In PTC tissues,the positive rate of FHIT gene mRNA expression was 45.0％ (27/60),lower than that in NCE tissues (100.0％,60/60).FHIT gene mRNA expression was correlated with pathology stage,TNM stage and lymph node metastasis (P ＜ 0.05).Conclusion FHIT loss and transcript abnormality might play important roles in proliferation and metastasis of PTC,and might be a useful marker for evaluating the biological behavior of PTC.

Objective To construct a]entiviral expression vector carrying CCDC67 and to obtain CCDC67 efficient and stable expression in mammalian cells.Methods The DNA fragment of CCDC67 coding sequence was amplified by PCR with designed primer from the cDNA library including CCDC67 gene,and then subcloned into GV208 vector with in-fusion technique to generate the lentiviral expression vector,GV208-CCDC67.The positive clones were screened by PCR and the correct CCDC67 was confirmed by sequencing.Recombinant lentiviruses were produced in 293T cells after the cotransfection of GV208-CCDC67,and packaging plasmids of pHelper 1.0 and pHelper 2.0 Green fluorescent protein (GFP)expression in 293T cells was observed to evaluate gene delivery efficiency.CCDC67 protein expression in 293T cells was detected by Western blot.Resuits The lentiviral expression vector carrying CCDC67 was successfully constructed and CCDC67 protein expression was detected in 293T cells.Conclusion The recombinant lentivirus GV208-GFP-CCDC67 is successfully constructed and it lays a foundation for further molecular function study of CCDC67.

Objective To investigate the relationship between thyroid and breast multiple primary carci-nomas.Methods A total of 24 cases of multiple primary tumors of thyroid and breast admitted from Jan .2011 to Jun.2013 in First Affiliated Hospital of Zhengzhou University were retrospectively analyzed .Results The 24 samples were confirmed as the tumor of thyroid and breast by ultrasonography .The patients underwent operation and adjuvant therapy after fine-needle aspiration biopsy , mammography and other relevant examinations .All the malignancies of thyroid and breast were confirmed by pathology .Among the 24 cases, 19 patients were invasive ductal carcinomas , 2 patients were diagnosed as intraductal carcinomas , 1 patient was confirmed as intraductal papillary carcinoma , 1 case was invasive lobular carcinoma with invasive ductal carcinoma , and 1 case was inva-sive ductal carcinoma with intraductal carcinoma .All the thyroid malignancies were papillary thyroid carcinomas . The results of immunohistochemistry indicated that , among the 24 patients of thyroid and breast multiple primary carcinomas , 11 cases were ER (+) in both thyroid cancers and breast cancers , moreover , 8 cases were ER (-) in both thyroid cancers and breast cancers .The difference between the expression of ER in the tumors of thyroid and breast had no statistical significance (P>0.05).Conclusions Ultrasonography is a simple and effective im-aging method that can discover the multiple primary tumors of thyroid and breast .For patients of thyroid cancer , breast cancer should be excluded routinely .The thyroid should also be examined simultaneously in breast cancer patients.Once the possibility of one gland tumor is discovered , we should pay more attention to the other gland . Particularly, the other gland should be examined carefully for patients with ER (+).

Objective:To explore the relationship between work connectivity behavior after-hours, self-regulatory fatigue and work engagement of nurses, so as to provide reference for developing strategies to improve nurses′work engagement.Methods:This was a cross-sectional survey study. A total of 661 nurses from 10 hospitals in Shandong Province were selected as subjects by convenience sampling method from June to August 2022. The Work Connectivity Behavior After-hours Scale, Self-Regulatory Fatigue Scale and Work Engagement Scale were used to measure the work connectivity behavior after-hours, self-regulatory fatigue level and work engagement level. A structural equation model was constructed to evaluate the mediating effect of self-regulatory fatigue on work connectivity behavior after-hours and work engagement.Results:The scores of work connectivity behavior after-hours, self-regulatory fatigue and work engagement of nurses were (37.66 ± 7.05), (42.98 ± 10.55) and (34.29 ± 6.58) points, respectively. The total effect of work connectivity behavior after hours on work engagement was -0.336 6. The work connectivity behavior after-hours was positively correlated with self-regulatory fatigue ( r = 0.423, P<0.01) and work engagement was negatively correlated with work connectivity behavior after-hours and self-regulatory fatigue ( r = -0.361, -0.479, both P<0.01). Self-regulatory fatigue had a partial mediating effect on work connectivity behavior after-hours and work engagement, accounting for 46.61% of the total effect. Conclusions:Self-regulatory fatigue has a mediating effect on the relationship between nurses′ work connectivity behavior after-hours and work engagement. Nursing managers should reduce the degree of self-regulatory fatigue of nurses by preventing their work connectivity behavior after-hours, so as to improve the work involvement level of nurses.