Animals ; Cerebral Cortex ; cytology ; physiology ; Delayed Rectifier Potassium Channels ; physiology ; Neurons ; physiology ; Patch-Clamp Techniques ; Rats ; Rats, Sprague-Dawley

Medical ethics has its own characteristics on curriculum setup,teaching goals,the structure of teaching staff in America,France and China,It is beneficial for our feform in education in medical ethics to learn and refer to the experiencs of the success abroad.

The rational design of teaching contents of physical pharmacy plays an important role in the class teaching.Some practices of designing teaching contents are expounded in detail,including the combination with teaching cases,the integration of the teaching contents,the combination with the research results of the physical pharmacy,and the combination with the pharmaceutical experiments of traditional Chinese medicine.The innovation of this research is the combination of theory and practice and the infiltration between teaching and scientific research.It also puts forward some measures such as continually accumulating and improving the related material,and constructing a rich,shared source material storehouse,etc..

Objective: To investigate the influencing factors for hemodynamics in lower limbs and provide information for prevention of deep venous thrombosis (DVT) in lower limbs after stroke. Methods: According to the degree of paralysis in limbs, the stroke patients were divided into muscle strength ≤3 grade group (n = 53) and > 3 grade group (n = 94), and the maximum venous outflow (MVO) in lower limbs was detected by pulse volume recorder. The relationship was analysed between MVO and age, blood pressure, muscle strength, cholesterol, glycerinate, plasma fibrinogen and level of plasma von Willerbrand factor (vWF). Results: The reduction of MVO is correlated with the degree of paralysis of lower limbs. There were negative correlation between MVO and the level of plasma vWF (r = -0.2571, P = 0.02). Conclusion: The level of plasma vWF and muscle strength has some effects on MVO in lower limbs during the acute stage after stroke.

Objective To predict the HLA A *0201 restricted CTL epitopes (nonamers) in SARS coronavirus (SARS CoV) E protein. Methods The CTL epitopes of E protein were predicted by using supermotif method combined with quantitative matrix method. Results Thirteen HLA A *0201 restricted CTL epitope candidates were predicted in SARS CoV E protein. Conclusion The prediction of the CTL epitopes in SARS CoV E protein will benefit the identification of CTL epitopes by experiment. Those results are of importance for immune recognition and vaccine design for SARS CoV.

Objective To detect DNA lesions induced by complex pollutants existing in environment using the technique of randomized terminal linker,dependent PCR (RDPCR). Methods SD rats were treated with organic concentrate by injection i.p., DNA were extracted from rat's liver, kidney, lung and leucocytes, then RDPCR was used to detect DNA damage of exon 7 of p53 gene. Results Organic concentrate of drinking water could make rat's genomic DNA broken. Hybridization bands were found in liver and kidney tissues after RDPCR, none in blood and lung tissues. Conclusion Organic concentrate of drinking water can cause the DNA lesions of exon 7 of p53 gene and the major target organs are liver and kidney. The technique of RDPCR can be used to detect DNA damage induced by complex environmental pollutants.

Blood Vessels ; injuries ; Humans ; Iatrogenic Disease ; prevention & control ; Vascular Diseases ; etiology ; prevention & control

An online questionnaire to 103 professionals of traditional Chinese medicine (TCM) was conducted to acquire their recognition and understanding of international communicative competence. The results showed that foreign language communicative competence and solid medical knowledge are considered the most important qualities while the importance of global vision, strategic thinking, cross-cultural awareness/sensitivity and communicative strategy is comparatively underestimated. It was proposed that domestic TCM universities and colleges strive to improve the soft skills for TCM professionals and cultivate more interdisciplinary TCM talents armed with professional expertise, foreign language communicative competence as well as international and multicultural awareness.

The specific aspects of big data management practice for the Million Veteran Program (MVP) in USA were summarized by investigating it with network survey method and literature survey method, including large scale collection of data, structure of data management organizations, platform for data integration, privacy and security of data.The enlightenments of big data management practice for the MVP in USA on big data management of precision medicine in China were analyzed from 5 aspects.

Objective:To make clear which drugs can cause drug eruption.Methods:Clinical analysis was made based on the patients data regarding the types of the drup eruption and the severity of liver impairment.Results:There are four types of drugs which can cause drug eruption:antibiotics (55.3%),antipyretic analgesic(26.6%)，Chinese medicine (9.6%) and biological preparations (8.5%).The drug eruptions usually manifested as unticaria,measles-like eruption,multiform erythma and stable eruption.Conclusion:The main allergic drugs are antibiotics and antipyretic analgesic.However,attention should also be payed to the Chinese medicine and biological preparations.