Objective To recognize normal sinus rhythm (NSR), ventricular tachycardia (VT) and ventricular fibrillation (VF) from each other accurately and promptly. Methods A nonlinear descriptor based on multi-scale analysis, Hurst index, as a feature to quantify the nonlinear dynamics behavior of the ECG signal, was quoted in this paper. Results The nonlinear technique, Hurst index was examined and evaluated with ECG signals extracted from MIT-BIH Arrhythmia Database, MIT-BIH Malignant Ventricular Ectopy Database, and CU Ventricular Tachyarrhythmia Database under a specific moving-window length. The experiment showed good performance of this nonlinear descriptor. When the window length was 5 s long, the recognition accuracy for each of NSR, VT and VF was 100%. Besides, the computing speed was much faster than the speed obtained with a traditional non-linear technique, the complexity measure algorithm. Conclusion The Hurst index has a strong potential for life-threatening ventricular arrhythmia recognition in clinical applications.

Objective To observe the effect of Yiqi Huoxue TCM combined with L-OHP+CF+5-FU regimen in treating advanced colorectal cancer patients. Methods 68 advanced colorectal cancer patients were randomly assigned to treatment group (38 patients, treated by Yiqi Huoxue TCM combined with L-OHP+CF+5-FU) and control group (30 patients, treated with L-OHP+CF+5-FU regimen). The therapeutic effects of two groups were observed. Result Clinical beneficial rate (CR+PR+SD) of treatment group and control group were 78.9% and 53.3% respectively (P

Objective:To explore antitumor mechanism of a recombinant vaccinia virus expressing human carcinomembryonic antigen(rV CEA).Methods:C57bl/6 mice were immunized three times with rV CEA.Six weeks later,the splenocytes from rV CEA immunized donors were adoptively tranfered in sublethally irradiated CEA + HePa bearing recipients,meanwhile the antiumor effect of the donor splencytes in virtro was detected.Results:Splenocytes from rV CEA immunized donors expressed strong antiumor activity in CEA positive tumorbearing recipients,whereas W VV immunized and nonimmune donor cell did not.The antiumor activity was abolished when immune T cells were depleted before splencytes transfer.Conclusion:It was demonstrated that antiumor acivity induced by rV CEA was essentially mediated by immune T cells which were activated by CEA specific immune response.Adoptive immunotherapy strategies may have implications for both the study of rV CEA and the development of potential clinical applications for tumor immunotherapy.

Objective: To study the antifungal activity of triazole alcohols by introduction of cyclopropyl as side chain. Methods: Nine title compounds were synthesized and characterized by 1 HNMR, MS spectra and element analysis. Eight fungi were used for in vitro anti-fungal test. Results: All of the title compounds showed antifungal activities of different extents, especially to the deep infection ones,and they had a MIC value < 0.125 μg/ml against Candida albicans,showing an anti-fungal activity 4 times higher than that of fluconzole and similar to that of totraconazaole. Conclusion: 1-(1H-1,2,4-triazole-1-yl)-2-(2, 4-difluoro-phenyl)-3-[N-cyclopropyl-N-(3,4-dichlorobenzyl) amino]-2-propanols (6f) has a better activity ratio and is worth further studying.

Objective To observe effect of Sanshen granule preparation combined with MVP regimen in treating middle-late stage non-small cell lung cancer patients. Methods 152 middle-late stage non-small cell lung cancer patients were randomly assigned to treatment group (79 patients) and control group (73 patients). Results Clinical beneficial rate (CR+PR+SD≥6 Months) were 82.3% and 64.4% respectively (P

Objective To observe the expression of resistin mRNA and protein in adipose tissues of obese rats,and to explore the correlations between resistin and obesity,insulin resistance.Methods Thirty SD rats were divided into control group(n=15) and high-fat diet group(n=15).The rats in control group recieved common forage.The obese and insulin resistance models were induced with high-fat diet in SD rats.The plasma insulin level was determined by double antibody radioinimunity analysis,and automatic biochemistry analyzer in plement was used to detect the plasma free fatty acid.After 11 weeks,glucose tolerance test was carried out to determine blood glucose levels at intervals(0,30,60,90,120 min).Resistin mRNA from fat pads tissue was extracted by RT-PCR,and then its protein was detected by Western blot.The data were analyzed statistically by SPSS 11.5 software.Results After 11 weeks,the rats′ weight in high-fat diet group increased obviously than that of control group,as well as fasting blood glucose,free fat acid homeostasis model assessment of insulin resistance(HOMAIR) and the glucose tolerance in high-fat diet group reduced greatly.The resistin mRNA and protein in white adipose tissues were significantly higher in the obese mice than those of control group(Pa

The development of proteomic technique has sparked new focus on novel protein markers for many diseases including cancer. Urine is one of the most useful biofluids for routine testing to provide an ideal resource for the discovery of novel biomarkers, with the advantage over tissue biopsy samples due to the ease and the less invasive nature of urinary collection. This review summarizes urinary protein tumor markers found in prostate cancer, bladder cancer, lung cancer and breast cancer that have shown potential for urogenital and non-urogenital cancer, so as to provide reference on high sensitive and high specific urinary protein tumor markers for early prevention, early diagnosis and treatment of cancer.

A 56-year-old female patient of Han nationality presented with generalized erythema and vesicles for 6 days,as well as high fever for 2 days.Twenty days prior to hospitalization,the patient received surgical treatment combined with oral methazolamide and glucocorticoids for glaucoma.The patient had a history of allergy to sulfanilamides.On admission,the patient presented with generalized erythema,vesicles and occasional erosions with bilateral eyelid and oral involvement.Nikolsky's sign was positive.Wheezing sound was heard over the right lung.Genetic testing showed that HLA-B5901 allele was positive.The patient was diagnosed with methazolamide-induced toxic epidermal necrolysis (TEN) complicated by pneumonia,and managed with immunoglobulin (25 g/day,5 days),glucocorticoids (the largest dose equivalent to methylprednisolone 160 mg/day),fresh plasma,antibiotics,as well as other supporting and symptomatic treatments.The condition was controlled after 2 weeks,and the patient was cured and discharged from hospital after 25 days.The fact that the patient carried HLA-B5901 allele suggests that HLA-B5901 is strongly correlated with methazolamide-induced TEN or Stevens-Johnson syndrome in Chinese descendants or Han population,besides in Japanese and Korean descendants.

OBJECTIVE To construct a prokaryotic expression plasmid encoding HPV16E7-HSP70 fusion gene for further study on the immunity of HPV16E7- HSP70 fusion protein against laryngeal carcinoma. METHODS HPV16E7 was PCR-amplified,digested by NheI and SacI,and ligated into pET28a. HSP70 was cloned into pGEMTeasy,then recut from the vector by SalI and NotI and ligated into pET28a-HPV16E7. PCR amplification, restrict enzyme digestion, DNA sequencing, IPTG induction and Western Blot were used to identify the recombinant plasmid. RESULTS Double digestion and PCR amplification of the recombinant plas- mid have shown that the size of the inserted fragment is as expected. Sequence analysis has demonstrated that the inserted fragment encodes for the HPV16E7- HSP70 fusion gene. IPTG induction and Western Blot have shown that the fusion protein is expressed suc- cessfully in the prokaryotic expression plasmid. CONCLUSION The recombinant prokaryotic expression plasmid pET28a-HPV16E7-HSP70 has been con- structed successfully.

OBJECTIVE To study the expressions of heat shock protein 70 and human papillomavirus16E7 protein in human laryngeal squamous cell carcinoma, and their relationship in the genesis of human laryngeal squamous cell carcinoma. METHODS The expressions of HSP70 and HPV16E7 protein were detected by the immunohistochemical method in 78 specimens with laryngeal squamous cell carcinoma, 24 specimens with vocal cord polyps and 10 specimens of normal laryngeal tissues. RESULTS In human laryngeal squamous cell carcinoma, vocal cord polyps and normal laryngeal tissues, the positive expression rates of HSP70 were 69.2 % , 8.3 % and 0 % respectively, with those of HPV16E7 protein being 43.6 % 4.2% and 0 % respectively. There was a significant difference of the expression rate of HSP70 or HPV16E7 protein between the laryngeal squamous cell carcinoma and the vocal cord polyps(P