1.Extraction and purification of giant salamander skin mucous glycoprotein and study its anti-cancer activity of lung cancer
Weiliang XU ; Dejing CHEN ; Yu LIU ; Hong WEI ; Qing LIU
Chinese Journal of Biochemical Pharmaceutics 2015;(8):44-47
Objective To study the appearance of skin mucous glycoprotein in vitro on the activity of human lung cancer cells A549. Methods Used alkali extraction and DEAE-52 ion exchange chromatography and Sephadex G-100 gel chromatography purification salamander mucous glycoprotein; Salamander mucous glycoprotein inhibition of human lung cancer cells A549 was detected by MTT colorimetric method in vitro.ResuIts It showed that the total sugar content in the appearance of mucus was 4.23%, the relatively pure glycoprotein component, by SDS protein electrophoresis tests, it contained a single glycoprotein component, its molecular weight was about 30 kDa.With glycoprotein pure concentration increased from 1,10, 20,40μg/mL, the glycoprotein inhibition rate of A549 cells increased; when the glycoprotein concentration was 40 μg/mL, for 24 h action, the inhibition rate of A549 cells was up to 85.66 %, while the role of 48 h, the inhibition rate of A549 cells was up to 92.32%.Inhibition effect of mucus glycoprotein on A549 cell compared with positive control drug, had significant difference.ConcIusion Salamander mucus glycoproteins of human lung cancer cells has obvious inhibitory effect, can provide theoretical basis for the development of lung cancer drug resistance.
2.Inflammatory stress induces insulin resistance in CD36 knockout mice
Xian CHEN ; Qing LIU ; Han LEI ; Hong LIU
Journal of Third Military Medical University 2003;0(09):-
Objective To explore whether the inflammatory stress can increase insulin resistance in the CD36 knockout (KO) mice.Methods After the mice were fed a standard chow for 14 weeks,oral glucose tolerance test,insulin release tests,lipids metabolism,SAA,IL-6,TNF-? and hepatic mRNA and proteins expression of mTOR,S6K,IRS-1,pIRS-1,2 were measured.Results Compared with the wide-type,the CD36 KO mice un-inflamed exhibited insulin resistance,and the insulin resistance index was increased[(3.01?1.24) vs (0.81?0.12),P
4.Chromatography-efficacy relation study between HPLC fingerprints and allelopathic effect of Salvia miltiorrhiza aqueous solution on radish.
Min NIU ; Hong-Yan LIU ; Jia LI ; Yong-qing ZHANG
China Journal of Chinese Materia Medica 2015;40(5):885-888
To explore the effective components represented by fingerprint contributed to allelopathic effect of different Salvia miltiorrhiza aqueous concentration on seeds and seedlings of radish, grey relational analysis was used to establish the chromatography-efficacy relation. The results show that 15 peaks devote high allelopathic contribution to radish seeds and seedlings. The study will provide a new concept for allelochemicals screening and study.
Allelopathy
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Chromatography, High Pressure Liquid
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Pheromones
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analysis
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metabolism
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pharmacology
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Plant Extracts
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analysis
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metabolism
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pharmacology
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Raphanus
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drug effects
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growth & development
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Salvia miltiorrhiza
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chemistry
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metabolism
5.Case of up-rushing gas syndrome and chest apoplexy.
Hong-Wei JI ; Qing-Zhang FENG ; Fang-Ming LIU
Chinese Acupuncture & Moxibustion 2013;33(10):904-904
Acupuncture Therapy
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Chest Pain
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therapy
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Female
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Humans
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Middle Aged
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Stroke
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therapy
6.Intravascular lymphomatosis of the prostate gland.
Xiu-rong YIN ; Hong LIU ; Hui-qing CHEN
Chinese Journal of Pathology 2005;34(3):189-190
Aged
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Antigens, CD20
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metabolism
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Humans
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Leukocyte Common Antigens
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metabolism
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Lymphoma, B-Cell
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complications
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metabolism
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pathology
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surgery
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Male
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Prostate
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pathology
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Prostatectomy
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Prostatic Hyperplasia
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complications
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metabolism
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pathology
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surgery
7.A clinical analysis of dermatomyositis with interstitial lung disease in 20 patients
Hong LI ; Qing LIN ; Hongmei JIAO ; Xinmin LIU
Chinese Journal of Internal Medicine 2012;51(3):214-216
Objective To investigate the clinical features of the patients with dermatomyositis (DM)complicated with interstitial lung disease(ILD).Methods The clinical manifestations and the laboratory data of the 20 patients with DM complicated with ILD were retrospectively analyzed.Results Among 169 DM patients,20(11.8%)were founded with ILD and 4 had no complaint of respiratory system.The ratio of male to female was 1∶2.33.The level of serum creatine kinase(CK)was elevated in 11 cases.Anti-Jo-1 antibody was positive in 4 cases.Bilateral infiltrates including ground-glass attenuation,interlobular septal thickening and reticular opacities were found by chest high resolution computed tomography.The impairment of lung function was manifested as the decreased diffusion capacity for carbon monoxide of the lung(DLCO)and restricted ventilatory function abnormalities.Fifteen cases showed hypoxemia or respiratory failure by arterial blood gas analysis.All patients were treated with glucocorticoid and/or immunosuppressive agents.Totally,14 patients had improved,while 2 patients with no improvement and 4 patients died with a mortality of 20%.Conclusions DM complicated with ILD which is easily missed has a high morbidity and mortality.The understanding of DM complicated with ILD should be enhanced,thus early diagnosis and intervention could be performed for better prognosis.
8.Clinical pathological features of umbilical metastases of colorectal carcinoma
Duju QING ; Zhijun GONG ; Gang KONG ; Yongquan LIANG ; Hong LIU
Journal of Chinese Physician 2011;(z1):16-18
Objective To study the clinical pathological features, treatment and prognosis of umbilical metastases of colorectal carcinoma. Methods From January 2000 to September 2010, 10 umbilical metastases of colorectal carcinoma cases were admitted. The clinical features were reviewed. Results Four radical resection of colon cancer and resection of the umbilical plexus metastases cases with chemotherapeutic intraperitoneal perfusion lived 9,11,14 and 18 months respectively, 1 ileum-transverse colon anastomosis case with chemotherapeutic intraperitoneal perfusion lived six months, 2 patients with systemic widely transfer and umbilical transfer with pure venous chemotherapy lived 3, 3.5 months respectively, 3 colon intra-operative cases with venous chemotherapy lived 5 ,5. 5 and 7 months respectively. Conclusion Surgical resection of the primary focal and periumbilical metastases can prolong survival time with adjunctive therapy.
9.Contrast-enhanced ultrasound of pulmonary carcinoma:a preliminary study
Zhiyan LUO ; Xueming LIU ; Qing WEN ; Juanjuan CHEN ; Yurong HONG
Chinese Journal of Ultrasonography 2008;17(8):690-693
Objective To explore the enhancement patterns of pulmonary carcinomas by contrast-enhanced ultrasound(CEUS).Methods Thirty-eight patients with pulmonary carcinomas proven by pathology[28 with peripheral pulmonary carcinomas and 10 central pulmonary carcinoma with obstructive atelectasis(OA)]were examined by baseline ultrasound and contrast-enhanced ultrasound,then the arrival time(AT),time to peak(TTP)were analyzed with time-intensity curve analysis software and the dynamic enhancement pattern of each lesion was assessed.Results Twenty-four peripheral pulmonary carcinomas demonstrated delayed AT about 6-16 s after application of contrast medium,three lesions demonstrated early AT about 4-5 s and one lesion demonstrated absence of contrast enhancement.The lesions exhibited hyper-,hypo- and non-enhancement were 14,13 and 1,respectively.Seventeen lesions were heterogenous enhanced with non-enhanced necrosis areas and enhanced septa,while ten lesions homogeneous enhanced and one lesion no enhanced.Ten central pulmonary carcinoma with OA demonstrated a characteristic pattern:OA appeared a short AT(mean AT 4.8 s)until enhancement and strong contrast enhancement,while the central tumors appeared a delayed AT(mean AT 10.5 s)and faint enhancement.Conclusions CEUS can be useful in differentiation between solid and cystic pulmonary lesions,and detection of the latent lesions underlying the atelectasis.
10.Clear cell chondrosarcoma: report of a case.
Hong-qi SHI ; Qing-wei LIU ; Shan-xian LOU
Chinese Journal of Pathology 2007;36(7):498-499
Adult
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Arthroplasty, Replacement, Hip
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Bone Cysts, Aneurysmal
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pathology
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Bone Neoplasms
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metabolism
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pathology
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surgery
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Chondroblastoma
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pathology
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Chondrosarcoma
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Female
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Femur Head
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metabolism
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pathology
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surgery
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Follow-Up Studies
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Humans
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Osteosarcoma
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pathology
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S100 Proteins
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metabolism