Objective To investigate the changes of thyroid hormones in pregnant women of different gestation by analyzing the test results from pregnant women .Methods Authors collected serum from 1 764 pregnant women and 121 healthy women(not pregnant ,control group) .And the levels of thyroid stimulating hormone(TSH) ,free triiodothyronine(FT3 ) and free thyroxine (FT4 ) in serum were tested by chemiluminescence microparticle immunization analysis(CMIA) method .Results The level of TSH (1 .06 mIU/L) in early-gestation women was lower than that in control group(P<0 .05) ,but increased in mid-gestation and late-gestation ,there was significant difference among early-gestation ,mid-gestation and late-gestation women(P<0 .05) .The levels of FT3 and FT4 in early-gestation women were higher than those in control group(P<0 .05) ,but decreased during mid-gestation and late-gestation ,there was significant difference among early-gestation ,mid-gestation and late-gestation women(P<0 .05) .Conclusion Thyroid hormones of pregnant women change in different gestation periods ,different reference values have to be adapted to evaluate thyroid gland function .

From 2003 to 2004,83 patients(166 sides) with nasal septum deviation were treated in our hospital,including 38 receiving submucous resection of nasal septum(SRNS) and 45 receiving submucous resection of nasal septum plus unilateral or bilateral partial inferior turbinectomy(SRNS+U/B PIT).All patients were tested by Eccovision acoustic rhinometry before and 6 months after operation to assess the treatment effect.It was found that total nasal cavity was bigger in the patients receiving SRNS+U/B PIT than in those receiving SRNS alone(P

Triple-negative breast cancer (TNBC) is a heterogeneous disease. Recently, the development of a gene expression profile fa-cilitated the re-classification of TNBC into six new subtypes, which show varied sensitivities to different therapies. In the era of preci-sion medicine, precision therapy may be directed at various potentially actionable molecular mutations in different subtypes of TNBC.

With the development of the next generation sequencing technology, considerable attention has been paid to the utility of circulating tumor DNA (ctDNA) detection in breast cancer. There are many clinical trials showed the ctDNA detection is a potential biomarker for the diagnosis, management and prognosis of breast cancer. ctDNA detection can provide a more accurate diagnosis for patients to guide clinical treatment in precision medicine era.

OBJECTIVE One of the objective of the study was to analyze the reliability and validity of the Chinese version of Glasgow children's benefit inventory (GCBI). The other was to explore whether the Chinese version of GCBI could be used as a appropriate evaluation method on children's quality of life (QOL) after tonsillectomy and adenoidectomy (T&A). METHODS Firstly, seventy-three eligible children and their proxies were interviewed with this survey scale, the survey scale was assessed by split-half reliability, internal consistency, content validity and construct validity. Second, the Chinese version of GCBI used to evaluate the changes of these 73 children's QOL at 12 to 18 months after operation. RESULTS First, the reliability coefficient and the construct validity of the Chinese version of GCBI was satisfactory. Second, almost all children's QOL changed better after operation, it improved the most in physical aspects. CONCLUSION The Chinese version of GCBI had good reliability and validity, and is suitable for assessing QOL of children after T&A. It can help clinician evaluate therapeutic effect in pediatric otolaryngology.

Objective:To study effect of transcatheter occlusion and minimally invasive surgical occlusion in treating secundum atrial septal defect. Methods:Patients with secundum atrial septal defect were collected and randomly divided into observation group given transcatheter occlusion and control group received minimally invasive surgical occlusion. Then operation related index and and heart function index were observed. Results: there were no differences in operation related index between two groups(x2=1.039, P>0.05);operation success had negative correlation with age and positive correlation with LEVF (t=7.832, P<0.05) and no correlation with sex(t=0.892, P>0.05);in patients older than 45, the successful case, LEVF and LVEDD of observation group were significantly higher than control group(t=5.372, t=5.382;P<0.05). Conclusion:transcatheter occlusion and minimally invasive surgical occlusion can achieve ideal therapeutic efficacy;transcatheter occlusion has positive clinical value in patients over 45 years old for its advantages of small operation wound.

The physiological and biochemical conditions of adults are relatively stable,while children are in the period of rapid growth and development with all kinds of physical and chemical indicators constantly changing,which brings great challenge to the pediatric clinical laboratories.In addition,children in different periods have different disease spectrum,among which many diseases are particular to children.This opens a new world for the pediatric laboratories.In this paper,a brief overview of problems,characteristics and future development of pediatric laboratory medicine is made.

Objective To develop a method to determine molybdenum in radix puerariae and the soil. Methods A method for determination of trace molybdenum in radix puerariae and the soil where it growth by single sweep polarography has been used. Results The linear range was 0.010-1.0 ?g/ml, r=0.999 0, the detection limit was 6.0?10-3 ?g/ml, the recovery rates were 98.0%～106.0%, RSD was 2.2%-4.8%. Conclusion This method is suitable for the determination of molybdenum in radix puerariae and the soil. The content of Mo in radix puerariae is related to that in the soil.

Objective: To investigate the protein expression of HPV16 and its early gene E6, E7 in human laryngeal squamous cell carcinoma tissues, and to analyze their relationship with the clinical stage and pathological classification of laryngeal squamous cell carcinoma. Methods: The expression of HPV16, HPV16 E6, and HPV16 E7 protein was detected in 147 specimens of different laryngeal lesions immunohistochemically. The specimens included 82 laryngeal carcinoma, 39 non-carcinoma tissues (including 27 specimens of vocal cord polyp and 12 specimens of normal laryngeal tissues taken from more than 1.0 cm adjacent to the carcinoma), and 26 precancerous lesions (leukoplakia) of the larynx. The relationship between the protein expression with the clinical stage and histopathological classification of laryngeal squamous cell carcinoma was analyzed. Results: The positive rates of HPV16, HPV16 E6, and HPV16 E7 protein in precancerous tissues (30.77%, 26. 92%, and 26. 92%, respectively) were significantly lower than those in laryngeal carcinoma lesions (45. 12%, 39. 02%, and 42. 68%, respectively; P<0. 05 or 0. 01), but were significantly higher than those in non-carcinoma tissues (23. 08%, 5. 13%, and 2.56%, respectively; P<0.05 or 0.01). In non-carcinoma tissues, the positive rate of HPV16 protein was significantly higher than that of E6 or E7 (P<0.05 or 0.01), while there was no difference between their positive rates in laryngeal carcinoma or precancerous lesions. We found that human laryngeal carcinoma tissues with different clinical stages and different pathological classifications also had different positive rate of HPV16 protein (P<0.05), but they had a similar positive rate of HPV16 E6 and E7. Conclusion: The expression of HPV16 E6, E7 proteins after the HPV16 infection might be one of the reasons for development of human laryngeal carcinoma. Inhibition of HPV16E7 expression by immunologic strategy may have a potential for treatment of laryngeal carcinoma.

From 2003 to 2004, 83 patients (166 sides) with nasal septum deviation were treated in our hospital, including 38 receiving submucous resection of nasal septum (SRNS) and 45 receiving submucous resection of nasal septum plus unilateral or bilateral partial inferior turbinectomy (SRNS+U/B PIT). All patients were tested by Eccovision acoustic rhinometry before and 6 months after operation to assess the treatment effect. It was found that total nasal cavity was bigger in the patients receiving SRNS+U/B PIT than in those receiving SRNS alone (P<0.05). It was also found that patients receiving SRNS had unsatisfactory and unstable outcomes. Therefore, it is suggested that patients suffering from deviation of nasal septum (caused by chronic hypertrophic rhinitis) should receive SRNS+U/B PIT. In order to standardize this surgical procedure, we named this operation as "nasal modification"; the operation indications and principles of it should be established and completed.