1.Effects of Compound Uncaria Hypotensive Tablets on Expressions of MCP-1 and MMP-9 of Vascular Remodeling in Spontaneously Hypertensive Rats
Jin FENG ; Wen ZHANG ; Jun QING ; Huimin LIU ; Yuansheng TAN
Chinese Journal of Information on Traditional Chinese Medicine 2016;23(10):51-55
Objective To observe the effects of Compound Uncaria Hypotensive Tablets on expressions of MCP-1 and MMP-9 of vascular remodeling in spontaneously hypertensive rats (SHR); To discuss it possible mechanism of action. Methods Totally 24 12-week old male SHR were randomly divided into SHR model group, Compound Uncaria Hypotensive Tablets group, and positive medicine group, with 8 rats in each group. Another 8 WKY rats were set as normal control group. Medication groups were given relevant medicine for gavage for successive 6 weeks. Noninvasive tail cuff method was used to observe blood pressure; morphological changes in thoracic aorta and renal artery were observed by HE staining; immunohistochemistry was used to detect the protein expressions of MCP-1 and MMP-9 in thoracic aortic wall. Results Protein expressions of MCP-1 and MMP-9 in thoracic aortic wall of SHR model group were significantly higher than those of normal control group (P<0.01); Compared with the SHR model group, protein expressions of MCP-1 and MMP-9 in thoracic aortic wall decreased significantly in the medication groups (P<0.05, P<0.01); Compared the Compound Uncaria Hypotensive Tablets group and positive medicine group, there was no obvious difference in protein expressions of MCP-1 and MMP-9 in thoracic aortic wall. Conclusion Compound Uncaria Hypotensive Tablets can reduce the blood pressure of SHR, reduce inflammation reaction, and regulate vascular remodeling, which mechanism may be related to down-regulation of expressions of MCP-1 and MMP-9 in SHR aortic endothelial cells.
2.The experimental research of R-Salbutamol on inhibiting the contraction of isolated tracheal smooth muscle in guinea-pig
Yun SUN ; Wen TAN ; Huazhu ZHOU ; Qing LIU
Chinese Pharmacological Bulletin 1987;0(02):-
Aim To evaluate the effects of R-Salbutamol(R-Sal)on the contraction of isolated tracheal strips and lung parenchyma strips in guinea-pig,induced by Histamine(His).Methods Tracheal strips and lung parenchyma strips of guinea-pig in vitro were prepared,and the dilatory effect on shrinkage reaction of isolating specimens induced by His were measured before or after the administration of R-Sal in doses of 10-8,10-7,10-6 mol?L-1,with fix-doses pharmacology methods.The inhibitory effect was compared with that of Sal(10-6 mol?L-1).Results His could induce the contraction of isolated strips in guinea-pig in a dose-dependent manner,and R-Sal could significantly inhibit this shrinkage of lung parenchyma induced by His.in a dose-dependent manner.R-Sal was much more efficient than Sal(P
3.Research advances of photosensitizer in photodynamic therapy of glioblastoma
Hong-cheng ZHAO ; Yue-qing WANG ; Qing-yun LI ; Hao DENG ; Xiao TAN ; Xiao-wen LIU
Acta Pharmaceutica Sinica 2022;57(6):1750-1757
Glioblastoma is a malignant tumor in central nervous system, which has strong invasion, poor prognosis and short survival time. At present, the main treatment strategy of glioblastoma is surgical excision, supplemented by radiotherapy and chemotherapy. However, due to incomplete resection and high recurrence rate, it is urgent to find novel therapeutic method for glioblastoma. Photodynamic therapy, as a promising non-surgical treatment, provides a new strategy for postoperative adjuvant therapy of glioblastoma. This review summarizes the mechanism and clinical application of photodynamic therapy mediated by various photosensitizers in glioblastoma, in order to provide help for the treatment of glioblastoma.
4.Identification of prototype foamy virus Bel1 nuclear localization signal and its corresponding importins.
Qing-Lin MA ; Miao YU ; Di LUO ; Juan TAN ; Wen-Tao QIAO
Chinese Journal of Virology 2014;30(4):346-352
Bel1, a transactivator of prototype foamy virus (PFV), plays pivotal roles in the replication of PFV. Previous studies have shown that Bel1 bears a nuclear localization signal (NLS), but its amino acid sequence remains unclear and the corresponding importins have not been identified. In this report, we inserted various fragments of Bel1 into an EGFP-GST fusion protein and investigated their subcellular localization by fluorescence microscopy. We found that the 215PRQKRPR221 fragment could direct nuclear localization, which accords with the consensus sequence K(K/R)X(K/R) of monopartite NLS. Point mutation experiments revealed that K218, R219, and R221 are essential for the nuclear localization of Bel1. The results of the GST-pulldown showed that the Bel1 fragment with residues 215-223, which bears the NLS, interacts with KPNA1, KPNA6, and KPNA7. This result suggests that KPNA1, KPNA6, and KPNA7 maybe involved in Bel1 nuclear translocation.
Cell Line
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Cell Nucleus
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genetics
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metabolism
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virology
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Humans
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Nuclear Localization Signals
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genetics
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metabolism
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Protein Binding
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Protein Transport
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Retroviridae Infections
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genetics
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metabolism
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virology
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Retroviridae Proteins
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chemistry
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genetics
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metabolism
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Spumavirus
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chemistry
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genetics
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physiology
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Trans-Activators
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chemistry
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genetics
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metabolism
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alpha Karyopherins
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genetics
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metabolism
5.New dicyclopeptides from Dianthus chinensis.
Jing HAN ; Zhe WANG ; Yu-Qing ZHENG ; Guang-Zhi ZENG ; Wen-Jun HE ; Ning-Hua TAN
Acta Pharmaceutica Sinica 2014;49(5):656-660
One new dicyclopeptide cyclo-(L-N-methyl Glu-L-N-methyl Glu) (1), together with one new natural dicyclopeptide cyclo-(L-methyl Glu ester-L-methyl Glu ester) (2), and two known dicyclopeptides cyclo-(L-methyl Glu ester-L-Glu) (3), and cyclo-(L-Glu-L-Glu) (4), were isolated from the aerial parts of Dianthus chinensis L. Their structures were determined by spectroscopic analyses and chemical methods.
Dianthus
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chemistry
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Plant Components, Aerial
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chemistry
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Plants, Medicinal
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chemistry
6.Effect of Montelukast on Inflammatory Factors in Children with Asthma
qing-ling, XIE ; wei, JIAO ; zhi-hong, WEN ; ying, TAN ; qiong-yan, HU
Journal of Applied Clinical Pediatrics 1994;0(04):-
Objective To investigate the effect of leukotriene receptor antagonist montelukast on inflammatory factors in children with asthma.Methods Eighty children with moderate asthma who aged 6-14 years old were randomly assigned to 3 treatment groups:5 mg once daily montelukast,or inhaled 100 ?g twice daily budesonide and 5 mg montelukast with inhaled budesonide 100 ?g per day.Each dose group received medicine for 12 weeks.Before starting therapy and 12 months later,clinical effects were observed,and pulmonary function was measured in patients simultaneously;concentrations of serum and sputum eosinophil cationic protein(ECP), IL-5 and TNF-? were measured respectively;also the peripheral blood eosinophil (Eos)was counted.Results Following treatment,clinical evaluation was improved and there were significant increases in pulmonary function in asthmatic children.Compared with control group,the levels of serum ECP,IL-5,TNF-? and blood Eos counting increased significantly in asthmatic children.The blood Eos counting was significantly correlated with ECP concentration in serum of children with asthma(P
7.The associations between idiosyncratic adverse drug reactions and HLA alleles and their underlying mechanism.
Qing WANG ; Hu MEI ; Yalan ZHANG ; Xianchao PAN ; Wen TAN ; Li CHAO
Acta Pharmaceutica Sinica 2013;48(6):799-808
With the advent of Twenty-First century, more and more genome-wide association studies (GWAS) showed that idiosyncratic adverse drug reactions (ADRs) were closely related with human leukocyte antigen (HLA) alleles, such as the associations of abacavir-HLA-B*5701, allopurinol-HLA-B*5801, and carbamazepine-HLA-B*1502, etc. To explore the mechanisms of these idiosyncratic drug reactions, hapten hypothesis, danger signal hypothesis, pharmacological interaction (P-I) concept and autoimmune mechanism are proposed. In this paper, recent GWAS studies on the HLA-mediated adverse drug reactions and underlying mechanism are reviewed in detail.
8.Effects of continuous inhaled corticosteroid of biochemical indexes of bone metabolism and bone mineral density in children with asthma
qing-ling, XIE ; zhi-hong, WEN ; ying, HUANG ; you-hua, CHEN ; gui-fang, TAN ; li-hua, SU ; li-ping, NONG ; qiong-yan, HU ; ying, TAN
Journal of Applied Clinical Pediatrics 1992;0(06):-
Objective To observe the influence of long-term treatment with inhaled corticosteroid on biochemical bone indexes and bone mineral density (BMD) in children with asthma. Methods The design was a randomized, paralleled group study with 3 low dose regiments of 100, 200,300 micrograms of budesonide per day in 45 children with asthma aged 5-8 years old for 12 months. Before inhaled corticosteroid therapy and 6th,12th month,clinical effects were observed and lung function(FEV1) was measured; concentration of serum osteocalcin(OST),insulin-like growth factor-1(IGF-1),bony alkaline phosphatase (BALP) and urinary deoxypyridinolin: creatinine (DPD/Cr) were measured; BMD was examined by dual energy X-ray absorptiometry. Results Clinical evaluation was improved and there was significant increase in FEV1 of asthmatic children. The amount of serum OST was slightly higher,yet no significant compared with that of normal control group. There was significant increase of serum BALP in asthmatic children after treatment; there was significant increase in serum IGF-1 of patients group after treatment compared with in normal children at the same age group; there was significant decrease in urinary DPD/Cr after treatment.There was no significant decrease in BMD before and after treatment at the hip (neck of femur , trochanter of femur ,Ward′s triangle),the lumber area of the spine (L2-4) and forearm (ultradis, distal). Conclusion Long-term treatment with low does corticosteroid dose not restrictedly affect bone metabolism and BMD in children with asthma.
9.Effects of salvianolic acid B preconditioning endothelial progenitor cells on expressions of myocardial genes in bone mesenchymal stem cells at the early cell differentiation stage of rats.
Jun-Zhen TAN ; Qing-Wen LI ; Ya-Yun NAN
Chinese Journal of Integrated Traditional and Western Medicine 2009;29(6):529-532
OBJECTIVETo investigate the effects of Salvianolic acid B preconditioned endothelial progenitor cells (EPCs) on the Nkx2.5 and GATA-4 gene expressions at the early stage of cell differentiation of bone mesenchymal stem cells (BMSc) transplanted into infarcted myocardium, in order to find out the best synergism for co-transplantation of the two kinds of cells.
METHODSBMSc and EPCs of rats were isolated and cultured, and rats were modeled into acute myocardial infarction (AMI) by left coronary artery ligation. Then the EPCs preconditioned with different concentrations of Salvianolic acid B and BMSc or DMEM medium were implanted into heart ischemia area. Expressions of Nkx2.5 and GATA-4 mRNA expressions in myocardium were detected by Real-time RT-PCR 4 weeks later.
RESULTSCompared with those in the non-implanted model rats' myocardium, the gene expression of Nkx2.5 and GATA-4 mRNA were significantly higher in all the transplantation receptive groups, comparisons between the implanted groups showed that the highest value of expressions (2. 654 +/- 0.606 of Nkx2.5 and 1.573 +/- 0.372 of GATA-4) displayed in the group contained more EPCs, for 8-fold to BMSc in volume.
CONCLUSIONBMSc can differentiate into cardiac muscle like cells, and condition of their differentiation is related with the degree of the internal environment improved.
Animals ; Benzofurans ; pharmacology ; Cells, Cultured ; Endothelial Cells ; cytology ; drug effects ; transplantation ; Gene Expression ; drug effects ; Male ; Mesenchymal Stem Cell Transplantation ; Myocardial Infarction ; metabolism ; therapy ; Myocardium ; metabolism ; Random Allocation ; Rats ; Rats, Wistar ; Stem Cell Transplantation ; Stem Cells ; cytology ; drug effects
10.Emodin inhibits dietary induced atherosclerosis by antioxidation and regulation of the sphingomyelin pathway in rabbits.
Zi-qing HEI ; He-qing HUANG ; Hong-mei TAN ; Pei-qing LIU ; Ling-zhi ZHAO ; Shao-rui CHEN ; Wen-ge HUANG ; Feng-ying CHEN ; Fen-fen GUO
Chinese Medical Journal 2006;119(10):868-870
Animals
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Antioxidants
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pharmacology
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Apoptosis
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drug effects
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Atherosclerosis
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prevention & control
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Ceramides
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analysis
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Dietary Fats
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administration & dosage
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Emodin
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pharmacology
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Lipids
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blood
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Male
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Rabbits
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Signal Transduction
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Sphingomyelin Phosphodiesterase
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metabolism
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Sphingomyelins
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metabolism