1.Effect of Angiotensin II Receptor Inhibitor on Proliferation and Apoptosis of a Human Endometrial Cancer Cell Line Ishikawa Induced by Estrogen
Journal of China Medical University 2010;(9):733-736
Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.
2.The standard of quality control of Yiqi Zhixue Granule
Chinese Traditional Patent Medicine 1992;0(03):-
AIM:To improve the standard of quality control of Yiqi Zhixue Granule(Radix Codonopsis,Radix Astragali,Rhizoma Atractylodis macrocephalae,etc.) METHODS: In the prescription,both Radix codonopsis and Radix Astragali were identified by HPLC.Folium mahoniae was distinguished by TLC.Rhizoma Bletillae was authenticated by microscope.Morever,RP-HPLC method was adopted to determine the astragaloside content in the productions,using a column of C_(18),the column temperature was at 35(?C),acetonitrile-water(32∶68) as a mobile phase,he flow rate was 1.0 mL/min,and ELSD as detection in which the tube temperature was set at 102(?C) and the air flow was at 2.8 mL/min,the impactor was chosen off. RESULTS: The established identification methods were simple and proper,reflecting a good specificity,while the determination method was accurate and reliable,which showed the recovery of 97.95%,RSD=2.21%(n=9),the intermediate precision of RSD=2.67%(n=3),the robustness of RSD=3.67%(n=3) between columns,and the LOQ of 0.539 2 ?g,RSD=2.92%(n=5). CONCLUSION: The improved standard of quality control of Yiqi Zhixue Granule is not only practical but capable of effectively controlling the quality of the medicine as well.
3.Clinicopathological study on the expression of p16 protein in thyroid tumor
Qing DING ; Xiufu QING ;
Chinese Journal of General Surgery 1997;0(06):-
Objective To study the relationship between the expression of p16 protein and the biological behavour of thyroid tumor. Methods The expression of p16 protein was examined in fifty cases of thyroid tumor by immunohistochemical staining technique(SABC method). The relationship between p16 protein expression and tumor size, pathological type, differention grading and clinical stage of thyroid tumors were analysed. Results In thyroid adenoma, p16 protein expression was detected in 13 out of 15 cases(86.7%); in thyroid carcinoma p16 protein was expressed only in 16 out of 35 cases(45.7%), the difference was significant(P
4.Study on present situation and safety of TCM injection
International Journal of Traditional Chinese Medicine 2012;34(3):227-228
Boosted in the rapid action,Traditional Chinese medical injection (TCMI) played an important role in the treatment of severe cases.In recent years,the safety of TCMI caused extensive concern of the domestic pharmaceutical indnstry.The clinical application of TCMI was analyzed in this paper to expound the existing security problem and to make reasonable suggestions for the evaluation.
5.Influence of systematic nursing on cancer related fatigue and life quality of patients with breast cancer during perioperative period
Chinese Journal of Practical Nursing 2012;28(28):27-29
Objective To discuss the influence of systematic nursing on cancer related fatigue and life quality of patients with breast cancer during perioperative period.Methods 197 patients in our hospital with breast cancer were randomly divided into the experimental group (99 cases)and the control group (98 cases)from November 2010 to December 2011.The experimental group was given systematic nursing method and the control group received traditional nursing.After treatment,the influence of nursing intervention on cancer related fatigue and QOL were compared between two groups.Results The score of QOL was(92.1±1.6)after nursing in the experimental group,higher than before systematic nursing and the control group.The severity of cancer related fatigue in the experimental group was apparently lower than before nursing intervention and that in the control group.There was significant difference.Conclusions The systematic nursing can reduce the level of perioperative cancer related fatigue and improve the life quality for the patients with breast cancer,so it can be widely used in clinic.
6.Advances on WT1, PRAME, ERG genes in acute myeloid leukemia
International Journal of Pediatrics 2011;38(4):390-392
Specific fusion genes of leukemia are currently the main biomakers for minimal residual disease (MRD)monitoring. Recent studys have found that the WT1, PRAME, ERG genes may be the potential acute leukemia related molecules, and can be used to monitor acute myeloid leukemia's MRD, directing clinical stratified therapy and estimating prognosis. This article reviews the pathogenesis, incidence of these genes and the relationship to the clinical prognosis.
7.Active cytomegalovirus infection before renal transplantation and it's affection on the patients
Journal of Chongqing Medical University 2007;0(09):-
Objective:To discover the matter of active Cytomegalovirus (CMV) infection before renal transplantation and its affection on the patients after transplantation. Methods:the numbers of pp65 positive cells in peripheral blood in 32 normal persons who were used as control and 156 uremia patients before renal transplantation were determined between June 1, 2006 and November 30, 2008. The variations of number of pp65 positive cells in peripheral blood in patients after renal transplantation were investgated. Results: In control group the number of pp65 positive cells in peripheral blood was 0/5?104 WBC. Before renal transplantation there was 12.2 percent of uremia patients whose pp65 positive cell counting was more than 0 cell /5?104 WBC.In this kind of patient the case number of serious active CMV infection(pp65 positive cell counting was more than 100 cells /5?104 WBC) and CMV disease after renal transplantation was more than others.The difference was significant. Conclusions: The findings suggest that active CMV infection before renal transplantation is an important sake that causes serious active CMV infection and even CMV disease after renal transplantation.
8.Analysis of 53 retinoblastoma cases in children
Journal of Chongqing Medical University 2003;0(05):-
Objective:To study the clinical characteristics of retinoblastoma in children.Methods:To analyze the age,sexuality,symptom,the accompaniment of symptom and the diagnostic feature of 53 retinoblastoma cases.Results:The average diagnostic age was two years and three months.There was no obvious sex difference.The principal first symptom of 24 cases was leukocoria,11 cases was strabismus,8 cases was big cornea or muddy cornea,6 cases was exophthalmos,1 case was nystagmus,2 cases was anterior chamber bleeding,and 1 case with health examination.It was significantly higher in single eye(85%) than in both eyes(15%).Conclusion:More and more people recognize the importance of eye health care,and the average diagnostic age becomes younger.The principal first symptom remains "galeamaurosis".The clinical characteristics of retinoblastoma in children are diversified?concealed?puzzling and easy to make missed diagnosis.
9.Image filtering technology in the application of B type ultrasonic diagnosis instrument
China Medical Equipment 2014;(11):36-38
Objective:Due to the inherent characteristics of medical ultrasound images, the study of B-type ultrasonic medical image filtering technique, you can make the image quality greatly improved.Methods: through tools such as Matlab, for b-mode ultrasonic image Adaptive mean filter, median filter, median filter, wavelet transform, anisotropic diffusion equation filtering;Results: compares the common b-mode ultrasonic filtering algorithms and research.Conclusion:all the filtering method for image signal is enhanced to a certain extent, suppression for noise, too. Mean square error reflects filtering image noise, while attention to detail, keep, for medical ultrasonic images in the hope that is in between the two to achieve an optimal balance.
10.Research on Theory of Mind in Autism
Chinese Mental Health Journal 2001;15(1):60-62
Objective: To investigate the understanding of other' s mind in children with autism in Beijing.Method: The instrument of this study was experimental test story. Results: Children with autism could understand other person' s physical and social desire, and predict behavior of others in accordance with desires. They basically unerstood other' s positive and negative emotions. However, they had a special difficult in understanding other' s cognitive mental states such as false belief. Concluslon: It indicated that the development of understanding other' s mental state is much more gradual and complex. Understandding other' s false belief is not all or nothing. The difference in understanding other's false belief between children with autism is not absolute, but relative.