Objective To study the relationship between the expression of p16 protein and the biological behavour of thyroid tumor. Methods The expression of p16 protein was examined in fifty cases of thyroid tumor by immunohistochemical staining technique(SABC method). The relationship between p16 protein expression and tumor size, pathological type, differention grading and clinical stage of thyroid tumors were analysed. Results In thyroid adenoma, p16 protein expression was detected in 13 out of 15 cases(86.7%); in thyroid carcinoma p16 protein was expressed only in 16 out of 35 cases(45.7%), the difference was significant(P

AIM:To improve the standard of quality control of Yiqi Zhixue Granule(Radix Codonopsis,Radix Astragali,Rhizoma Atractylodis macrocephalae,etc.) METHODS: In the prescription,both Radix codonopsis and Radix Astragali were identified by HPLC.Folium mahoniae was distinguished by TLC.Rhizoma Bletillae was authenticated by microscope.Morever,RP-HPLC method was adopted to determine the astragaloside content in the productions,using a column of C_(18),the column temperature was at 35(?C),acetonitrile-water(32∶68) as a mobile phase,he flow rate was 1.0 mL/min,and ELSD as detection in which the tube temperature was set at 102(?C) and the air flow was at 2.8 mL/min,the impactor was chosen off. RESULTS: The established identification methods were simple and proper,reflecting a good specificity,while the determination method was accurate and reliable,which showed the recovery of 97.95%,RSD=2.21%(n=9),the intermediate precision of RSD=2.67%(n=3),the robustness of RSD=3.67%(n=3) between columns,and the LOQ of 0.539 2 ?g,RSD=2.92%(n=5). CONCLUSION: The improved standard of quality control of Yiqi Zhixue Granule is not only practical but capable of effectively controlling the quality of the medicine as well.

Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.

Antibodies, Antineutrophil Cytoplasmic ; analysis ; immunology ; Antibodies, Antinuclear ; analysis ; immunology ; Antigens, Nuclear ; immunology ; Arthritis, Juvenile ; immunology ; Autoantibodies ; analysis ; immunology ; Humans ; Monitoring, Physiologic ; methods ; Nucleosomes ; immunology ; Phospholipids ; immunology ; Rheumatic Diseases ; immunology ; physiopathology

Specific fusion genes of leukemia are currently the main biomakers for minimal residual disease (MRD)monitoring. Recent studys have found that the WT1, PRAME, ERG genes may be the potential acute leukemia related molecules, and can be used to monitor acute myeloid leukemia's MRD, directing clinical stratified therapy and estimating prognosis. This article reviews the pathogenesis, incidence of these genes and the relationship to the clinical prognosis.

Invasive micropapillary carcinoma of breast is associated with poor prognosis and high incidence of lymph node metastasis.Tumor cells are observed as micro-papillary with light microscope.And high expression of epithelial membrane antigen(EMA) examined by immunohistochemistry is a reliable way of diagnosing this carcinoma accurately and timely.Compared with the previous diagnostic criteria,the new criteria pays more attention to the diagnosis of invasive micropapillary carcinoma of the breast.For this reason,it has important significance for treatment and prognosis of patients with invasive micropapillary carcinoma of the breast.

BACKGROUND: "Toxic impaired brain retinervus" is the important mechanism of acute cerebrovascular diseases, mainly presented by cerebral microvascular impairments.OBJECTIVE: To establish cell model of various degrees ischemia-reperfusion injury and expos to qingkailing medication, in order to observe the protective role of qingkailing injection on human umbilical venous endothelium ECV304 (huveECV304)DESIGN: Randomized controlled study based on cultured cells.SETTING: Department of Combination of Traditional Chinese Medicine and Western Medicine, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology.MATERIALS: This study was carried out at Center Laboratory of Dongzhimeu Traditional Chinese Medicine College, Beijing from February to April 2002. huveECV304 were randomly divided into 7 groups, namely normal group (NG), ischemic-ypoxia group (IR group), ischemia 6 hours reperfusion 12 hours control group (I-6-hour-R-12-hour group), ischemia 24 hours reperfusion 12 hours control group (I-24-hour-R-12-hour group),ishcmeia 24 hours reperfuiosn 12 hours low dosage qingkailing group (I24-hour-R-12-hour-LDQ group), ischemia 24 hours reperfusion 12 hours middle dosage qingkailing group (I-24-hour-R-12-hour-MDQ group), ischemia 24 hours reperfusion 12 hours high dosage qingkailing group (I-24hour-R-12-hour-HDQ group) and ischemia 6 hours reperfusion 12 hours middle dosage qingkailing group (I-6-hour-R-12-hour-MDQ group). The qingkailing injection mainly consists of cholaic acid, hyodeoxycholic acid,baikal skullcap root, cape jasmine fruit and so on.were collected, and primary culture medium were removed in I-6-hour-R-12hour group and I-24-hour-R-12-hour group and added with non-glucose Earle's solution, the cells in I-6-hour-R-12-hour group were cultured in ischemic incubator in 37 ℃ for 6 hours, for 24 hours in I-24-hour-R-12-hour group and then reperfusion-cultured with Earle's medium replaced by nonLDQ group, I-24-hour-R-12-hour-MDQ group and I-24-hour-R-12-hour-HDQ group, IR injured cells were exposed to qingkailing in dosage of 5, 10,by using MTT colorimetric assay, lactate dehydrogenase leaking rate was determined by using lactate dehydrogenase reagent kit, cell apoptosis rate was detected with FCM and statistically analyzed with Cellquest software.apoptosis rate.were found gathering closely, well adhering to the wall and completely stretching, cell surface was smooth and more cleavage cells were observed,cell grew over all the bottom displaying typical "slabstone signs"; but no obvious cell morphological changes were observed in control group and Ⅰ24-hour-R-12-hour LDQ group; while in I-24-hour-R-12-hour control group, cell were found scarcely distributed and mostly drop off, a great deal of tumid round injured cells and broken cell pieces were observed,adhering cells were shrunk obviously with pseudopod stretching out, less cleavage cells were observed; In I-24-hour-R-12-hour MDQ group, cells were found well adhering to the wall, the surface was smooth without cell: Comparing to I-24-hour-R-12-hour control group, the survival rate were markedly increased in I-24-hour-R-12-hour LDQ group, I-24-hour-R12-hour MDQ group [(42.5±2.8), (72.9±8.0), (80.3±12.7)%, P ＜ 0.01], but obviously descended to (10.1 ±0.4)% (P ＜ 0.01) in I-24-hour-R-12-hour ing rate of ECV304 cell: Comparing to I-24-hour-R-12-hour control group,it was found markedly increased in I-24-hour-R-12-hour LDQ group and Ⅰ24-hour-R-12-hour MDQ group [(6.6±1.4), (2.4±1.4), (2.4±0.5)%, P ＜ 0.01], but significantly ascended to (16.1±2.2)% in I-24-hour-R-12-hour rate of ECV304 cell: Comparing to I-6-hour-R-12-hour control group, it was obviously increased in comparing to I-6-hour-R-12-hour MDQ group [(2.2±0.5), (1.5±0.4)%, P ＜ 0.05]; comparing to normal group, it was found significantly increased in I-24-hour-R-12-hour control group and I-24-hourR-12-hour MDQ group [(0.9±0.2), (5.5±2.5), (9.9±4.3)%, P ＜ 0.01]; Moreover the increment in I-24-hour-R-12-hour control group was significantly higher than I-24-hour-R-12-hour MDQ group (P ＜ 0.01).ECV304 with various degree IR injury, but which displaying tow opposite cells due to I-6-hour-R-12-hour exposure was mainly presented by lowered cell apoptosis rate; But severer injury of ECV304 cells due to I-24-hour-R12-hour exposure was presented by increased cell apoptosis rate and lowon and cell membrane would not break in apoptostic cells and no internal content effused, therefore the surrounding cells would not be affected,which increasing the survival rate and displaying protective role for most cells.

Objective: To investigate the effect of different therapies on 159 children with anorexia caused by non-disease factors. Methods: Chinese traditional medicine therapy (CTMT) and psychotherapy and the combination of CTMT and psychotherapy were applied to three groups of anorexia children respectively. Results: The cure rate of the CTMT group was 27.5%, the psychotherapy group was 31.8%, and the combination group was 89.3%. Conclusion: The combination of psychotherapy and Chinese traditional medicine was much more effective than psychotherapy or Chinese traditional medicine alone in the treatment of anorexia in children.

Objective: To investigate the understanding of other' s mind in children with autism in Beijing.Method: The instrument of this study was experimental test story. Results: Children with autism could understand other person' s physical and social desire, and predict behavior of others in accordance with desires. They basically unerstood other' s positive and negative emotions. However, they had a special difficult in understanding other' s cognitive mental states such as false belief. Concluslon: It indicated that the development of understanding other' s mental state is much more gradual and complex. Understandding other' s false belief is not all or nothing. The difference in understanding other's false belief between children with autism is not absolute, but relative.

There are numerous complex mechanisms contributing to the evolution of septic shock, and early diagnosis and intervention will improve the prognosis of patient with septic shock. Recently plasma concentrations of some bio-markers like pro-ADM,pro-ANP,pro-ET1 and copeptin are found increasing in patient with septic shock.The plasma concentrations of these bio-markers are positively related with the severity of infection.These bio-markers have long halflife and are easily assayed. They may be the new bio-markers for the early diagnosis of septic shock.