Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.

Objective To study the relationship between the expression of p16 protein and the biological behavour of thyroid tumor. Methods The expression of p16 protein was examined in fifty cases of thyroid tumor by immunohistochemical staining technique(SABC method). The relationship between p16 protein expression and tumor size, pathological type, differention grading and clinical stage of thyroid tumors were analysed. Results In thyroid adenoma, p16 protein expression was detected in 13 out of 15 cases(86.7%); in thyroid carcinoma p16 protein was expressed only in 16 out of 35 cases(45.7%), the difference was significant(P

AIM:To improve the standard of quality control of Yiqi Zhixue Granule(Radix Codonopsis,Radix Astragali,Rhizoma Atractylodis macrocephalae,etc.) METHODS: In the prescription,both Radix codonopsis and Radix Astragali were identified by HPLC.Folium mahoniae was distinguished by TLC.Rhizoma Bletillae was authenticated by microscope.Morever,RP-HPLC method was adopted to determine the astragaloside content in the productions,using a column of C_(18),the column temperature was at 35(?C),acetonitrile-water(32∶68) as a mobile phase,he flow rate was 1.0 mL/min,and ELSD as detection in which the tube temperature was set at 102(?C) and the air flow was at 2.8 mL/min,the impactor was chosen off. RESULTS: The established identification methods were simple and proper,reflecting a good specificity,while the determination method was accurate and reliable,which showed the recovery of 97.95%,RSD=2.21%(n=9),the intermediate precision of RSD=2.67%(n=3),the robustness of RSD=3.67%(n=3) between columns,and the LOQ of 0.539 2 ?g,RSD=2.92%(n=5). CONCLUSION: The improved standard of quality control of Yiqi Zhixue Granule is not only practical but capable of effectively controlling the quality of the medicine as well.

Antibodies, Antineutrophil Cytoplasmic ; analysis ; immunology ; Antibodies, Antinuclear ; analysis ; immunology ; Antigens, Nuclear ; immunology ; Arthritis, Juvenile ; immunology ; Autoantibodies ; analysis ; immunology ; Humans ; Monitoring, Physiologic ; methods ; Nucleosomes ; immunology ; Phospholipids ; immunology ; Rheumatic Diseases ; immunology ; physiopathology

The clinical manifestations of infectious retinal diseases are complicated,especially these result from serious infectious diseases such as acquired immune deficiency syndrome (AIDS),tuberculosis and syphilis infections.It is an important issue to differentiate infectious retinal disease from non-infectious intraocular inflammation in the clinic.It is,therefore,highly desirable to follow a proper steps to reach the correct diagnosis.Complete history review and comprehensive ocular examination remains the first step in diagnosing infectious retinal diseases.Although an array of laboratory and serological tests are available to assist in the diagnosis,some situations may require a diagnostic therapy or a tissue biopsy.Identification of the pathogen and histopathologic examination of the ocular specimen remain to be the gold standard of diagnosis.Initiation a specific and appropriate antimicrobial therapy needs multidisciplinary collaborations including ophthalmologists and infectious specialists.Updated knowledge of general medicine and management of infectious diseases,interdisciplinary collaborations and optimization of treatment processes will improve the diagnosis and treatment of retinal infectious diseases in the future.

Objective: To investigate the effect of different therapies on 159 children with anorexia caused by non-disease factors. Methods: Chinese traditional medicine therapy (CTMT) and psychotherapy and the combination of CTMT and psychotherapy were applied to three groups of anorexia children respectively. Results: The cure rate of the CTMT group was 27.5%, the psychotherapy group was 31.8%, and the combination group was 89.3%. Conclusion: The combination of psychotherapy and Chinese traditional medicine was much more effective than psychotherapy or Chinese traditional medicine alone in the treatment of anorexia in children.

Objective:Due to the inherent characteristics of medical ultrasound images, the study of B-type ultrasonic medical image filtering technique, you can make the image quality greatly improved.Methods: through tools such as Matlab, for b-mode ultrasonic image Adaptive mean filter, median filter, median filter, wavelet transform, anisotropic diffusion equation filtering;Results: compares the common b-mode ultrasonic filtering algorithms and research.Conclusion:all the filtering method for image signal is enhanced to a certain extent, suppression for noise, too. Mean square error reflects filtering image noise, while attention to detail, keep, for medical ultrasonic images in the hope that is in between the two to achieve an optimal balance.

Objective To investigate the risk factors and clinical features of severe preeclampsia(SP) complicated with multiple organ dysfunction syndrome (Multiple Organ Dysfunction Syndrome,MODS) in order to provide reference for clinical practice.Methods Three hundred and ninety-one cases with severe preeclampsia were selected as our subjects,who hospitalized from February 2005 to February 2014 in our hospital.All subjects were divided into observation group (48 cases with MODS) and control group (343 cases without MODS).Clinical data were collected including age,gravidity,parity,gestational age,gestational age termination of pregnancy,uterine scar,prenatal care,medical history,treatment,mother child outcomes.Results There were significant differences between two groups in terms of weeks of termination of pregnancy,rate of uterine scar,rate of regular check-up of pregnancy,rate of chronic hypertension and renal disease.Logistic results showed that there were relationships between SP complicated with MO)S and the occurrence of termination of pregnancy with gestational age ＜28 weeks (OR =2.046,95％ CI:1.03 ～ 3.74,P =0.034),uterine scar (OR =2.401,95％ CI:1.16 ～ 4.09,P =0.008),non-regular prenatal care (OR =3.167,95％ CI:1.96 ～ 5.23,P =0.002),chronic hypertension (OR =3.348,95％ CI:2.23 ～ 5.78,P =0.001),renal disease (OR =3.064,95％ CI:1.77 ～ 5.18,P =0.038).The rate of underwent special treatment,preterm children,neonatal asphyxia,perinatal death,intracranial hemorrhage in observation group were 77.08％,79.17％,29.17％,20.83％,4.17％,higher than that in control group (23.62％,45.19％,13.70％,8.45％,1.17％ respectively; x2 =10.471,12.147,4.519,8.644,6.648,all P ＜ 0.05).The degree of organ injury score in gestational age ≥ 33 weeks and ＜ 33 weeks were (1.34 ± 0.28) points and (3.64 ± 0.69) points,and the difference was significant.The gestational week in gestational age ≥ 33 weeks and ＜33 weeks were (35.48 ±2.06) weeks and (29.87 ± 1.37) weeks,and the differencewas significant(t =3.917,P ＜0.05).The survival rates were 100％,77.78％,33.33％ when the numbers of damaged organs were 2,3 and ≥ 4.There was significant different between 2 and 3 damaged organs in terms of survival rate (x2 =3.964,P =0.024).Conclusion The risk factors of severe preeclampsia complicated with MODS include termination of pregnancy gestational age ＜ 28 weeks,uterine scar,non-regular prenatal care,chronic hypertension,renal disease.Prognosis of severe preeclampsia patients with MODs was poor than that without MODS and it relates with gestational age,the number of closely related organ damage.

Objective To put forward suggestions for individuals, hospitals and medical insurance institutions in protecting medical insurance information privacy by investigating the protection of individual medical insurance pri-vacy and cognition of information sharing. Methods The need of 200 insurants for protecting their medical insur-ance information privacy was investigated with questionnaires. Results The insurants were of certain awareness in protecting their medical insurance information privacy, and believed that hospitals and medical insurance institu-tions would take measures to protect their medical insurance information privacy. However, they would not like to share their medical insurance information because they were afraid of letting them out. The awareness in protecting medical insurance information privacy and sharing medical insurance information was different in the insurants en-gaged in different works with different levels of education. Conclusion The risk of letting medical insurance infor-mation out is existed in certain hospitals and medical insurance institutions due to the problems in the management of their medical insurance information system.

Objective To investigate the changes and clinical indications of interleukin-2 receptor, ferritin and fi-brinectin in the cerebrospinal fluid of central nervous system leukemia.Methods ELISA was used to detect the changes of-soluble interleukin-2 receptor, ferritin and fibrinectin in the cerebrospinal fluid of central nervous system leukemia group ( CNSL) and non-CNSL group.Results The concentrations of soluble interleukin-2 receptor, ferritin and fibronectin ince-rebrospinal fluid of the CNSL group were significantly higher than those in non -CNSL group and the control group ( p <0.05).Conclusion The soluble interleukin 2 receptor, ferritin and fibronectin in cerebrospinal fluid are indicative of cen-tral nervous system leukemia.The result suggests that combined detection is important in early diagnosis of central nervous system leukemia.