Objective To study the relationship between the expression of p16 protein and the biological behavour of thyroid tumor. Methods The expression of p16 protein was examined in fifty cases of thyroid tumor by immunohistochemical staining technique(SABC method). The relationship between p16 protein expression and tumor size, pathological type, differention grading and clinical stage of thyroid tumors were analysed. Results In thyroid adenoma, p16 protein expression was detected in 13 out of 15 cases(86.7%); in thyroid carcinoma p16 protein was expressed only in 16 out of 35 cases(45.7%), the difference was significant(P

Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.

AIM:To improve the standard of quality control of Yiqi Zhixue Granule(Radix Codonopsis,Radix Astragali,Rhizoma Atractylodis macrocephalae,etc.) METHODS: In the prescription,both Radix codonopsis and Radix Astragali were identified by HPLC.Folium mahoniae was distinguished by TLC.Rhizoma Bletillae was authenticated by microscope.Morever,RP-HPLC method was adopted to determine the astragaloside content in the productions,using a column of C_(18),the column temperature was at 35(?C),acetonitrile-water(32∶68) as a mobile phase,he flow rate was 1.0 mL/min,and ELSD as detection in which the tube temperature was set at 102(?C) and the air flow was at 2.8 mL/min,the impactor was chosen off. RESULTS: The established identification methods were simple and proper,reflecting a good specificity,while the determination method was accurate and reliable,which showed the recovery of 97.95%,RSD=2.21%(n=9),the intermediate precision of RSD=2.67%(n=3),the robustness of RSD=3.67%(n=3) between columns,and the LOQ of 0.539 2 ?g,RSD=2.92%(n=5). CONCLUSION: The improved standard of quality control of Yiqi Zhixue Granule is not only practical but capable of effectively controlling the quality of the medicine as well.

Invasive micropapillary carcinoma of breast is associated with poor prognosis and high incidence of lymph node metastasis.Tumor cells are observed as micro-papillary with light microscope.And high expression of epithelial membrane antigen(EMA) examined by immunohistochemistry is a reliable way of diagnosing this carcinoma accurately and timely.Compared with the previous diagnostic criteria,the new criteria pays more attention to the diagnosis of invasive micropapillary carcinoma of the breast.For this reason,it has important significance for treatment and prognosis of patients with invasive micropapillary carcinoma of the breast.

BACKGROUND: "Toxic impaired brain retinervus" is the important mechanism of acute cerebrovascular diseases, mainly presented by cerebral microvascular impairments.OBJECTIVE: To establish cell model of various degrees ischemia-reperfusion injury and expos to qingkailing medication, in order to observe the protective role of qingkailing injection on human umbilical venous endothelium ECV304 (huveECV304)DESIGN: Randomized controlled study based on cultured cells.SETTING: Department of Combination of Traditional Chinese Medicine and Western Medicine, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology.MATERIALS: This study was carried out at Center Laboratory of Dongzhimeu Traditional Chinese Medicine College, Beijing from February to April 2002. huveECV304 were randomly divided into 7 groups, namely normal group (NG), ischemic-ypoxia group (IR group), ischemia 6 hours reperfusion 12 hours control group (I-6-hour-R-12-hour group), ischemia 24 hours reperfusion 12 hours control group (I-24-hour-R-12-hour group),ishcmeia 24 hours reperfuiosn 12 hours low dosage qingkailing group (I24-hour-R-12-hour-LDQ group), ischemia 24 hours reperfusion 12 hours middle dosage qingkailing group (I-24-hour-R-12-hour-MDQ group), ischemia 24 hours reperfusion 12 hours high dosage qingkailing group (I-24hour-R-12-hour-HDQ group) and ischemia 6 hours reperfusion 12 hours middle dosage qingkailing group (I-6-hour-R-12-hour-MDQ group). The qingkailing injection mainly consists of cholaic acid, hyodeoxycholic acid,baikal skullcap root, cape jasmine fruit and so on.were collected, and primary culture medium were removed in I-6-hour-R-12hour group and I-24-hour-R-12-hour group and added with non-glucose Earle's solution, the cells in I-6-hour-R-12-hour group were cultured in ischemic incubator in 37 ℃ for 6 hours, for 24 hours in I-24-hour-R-12-hour group and then reperfusion-cultured with Earle's medium replaced by nonLDQ group, I-24-hour-R-12-hour-MDQ group and I-24-hour-R-12-hour-HDQ group, IR injured cells were exposed to qingkailing in dosage of 5, 10,by using MTT colorimetric assay, lactate dehydrogenase leaking rate was determined by using lactate dehydrogenase reagent kit, cell apoptosis rate was detected with FCM and statistically analyzed with Cellquest software.apoptosis rate.were found gathering closely, well adhering to the wall and completely stretching, cell surface was smooth and more cleavage cells were observed,cell grew over all the bottom displaying typical "slabstone signs"; but no obvious cell morphological changes were observed in control group and Ⅰ24-hour-R-12-hour LDQ group; while in I-24-hour-R-12-hour control group, cell were found scarcely distributed and mostly drop off, a great deal of tumid round injured cells and broken cell pieces were observed,adhering cells were shrunk obviously with pseudopod stretching out, less cleavage cells were observed; In I-24-hour-R-12-hour MDQ group, cells were found well adhering to the wall, the surface was smooth without cell: Comparing to I-24-hour-R-12-hour control group, the survival rate were markedly increased in I-24-hour-R-12-hour LDQ group, I-24-hour-R12-hour MDQ group [(42.5±2.8), (72.9±8.0), (80.3±12.7)%, P ＜ 0.01], but obviously descended to (10.1 ±0.4)% (P ＜ 0.01) in I-24-hour-R-12-hour ing rate of ECV304 cell: Comparing to I-24-hour-R-12-hour control group,it was found markedly increased in I-24-hour-R-12-hour LDQ group and Ⅰ24-hour-R-12-hour MDQ group [(6.6±1.4), (2.4±1.4), (2.4±0.5)%, P ＜ 0.01], but significantly ascended to (16.1±2.2)% in I-24-hour-R-12-hour rate of ECV304 cell: Comparing to I-6-hour-R-12-hour control group, it was obviously increased in comparing to I-6-hour-R-12-hour MDQ group [(2.2±0.5), (1.5±0.4)%, P ＜ 0.05]; comparing to normal group, it was found significantly increased in I-24-hour-R-12-hour control group and I-24-hourR-12-hour MDQ group [(0.9±0.2), (5.5±2.5), (9.9±4.3)%, P ＜ 0.01]; Moreover the increment in I-24-hour-R-12-hour control group was significantly higher than I-24-hour-R-12-hour MDQ group (P ＜ 0.01).ECV304 with various degree IR injury, but which displaying tow opposite cells due to I-6-hour-R-12-hour exposure was mainly presented by lowered cell apoptosis rate; But severer injury of ECV304 cells due to I-24-hour-R12-hour exposure was presented by increased cell apoptosis rate and lowon and cell membrane would not break in apoptostic cells and no internal content effused, therefore the surrounding cells would not be affected,which increasing the survival rate and displaying protective role for most cells.

Etiological and serological testing, including virus genome, virus antigen, anti-virus antibodies, virus culture and identification, were the standard laboratory tests to confirm or exclude Ebola virus infections.Moreover, other laboratory tests, such as blood routine tests, liver and kidney function tests, serum electrolyte tests, and coagulation function tests, were very important to the clinical diagnosis and treatment of Ebola virus disease ( EVD) patients.Based on working experiences and practices in the China Ebola Treatment Center and available publications, opinions about the detailed clinical significance and associated key point of biosafty prevention and protection protocols of above described laboratory tests for the clinical diagnosis and treatment of EVD were provided.

Objective To investigate the changes of thyroid hormones in pregnant women of different gestation by analyzing the test results from pregnant women .Methods Authors collected serum from 1 764 pregnant women and 121 healthy women(not pregnant ,control group) .And the levels of thyroid stimulating hormone(TSH) ,free triiodothyronine(FT3 ) and free thyroxine (FT4 ) in serum were tested by chemiluminescence microparticle immunization analysis(CMIA) method .Results The level of TSH (1 .06 mIU/L) in early-gestation women was lower than that in control group(P<0 .05) ,but increased in mid-gestation and late-gestation ,there was significant difference among early-gestation ,mid-gestation and late-gestation women(P<0 .05) .The levels of FT3 and FT4 in early-gestation women were higher than those in control group(P<0 .05) ,but decreased during mid-gestation and late-gestation ,there was significant difference among early-gestation ,mid-gestation and late-gestation women(P<0 .05) .Conclusion Thyroid hormones of pregnant women change in different gestation periods ,different reference values have to be adapted to evaluate thyroid gland function .

Objective:Due to the inherent characteristics of medical ultrasound images, the study of B-type ultrasonic medical image filtering technique, you can make the image quality greatly improved.Methods: through tools such as Matlab, for b-mode ultrasonic image Adaptive mean filter, median filter, median filter, wavelet transform, anisotropic diffusion equation filtering;Results: compares the common b-mode ultrasonic filtering algorithms and research.Conclusion:all the filtering method for image signal is enhanced to a certain extent, suppression for noise, too. Mean square error reflects filtering image noise, while attention to detail, keep, for medical ultrasonic images in the hope that is in between the two to achieve an optimal balance.

In this paper , the author analyzed the network media with three main characteristics of negative re-ports about doctor -patient dispute , from four aspects explains the network media in building a harmonious doctor-patient relationship in ethics responsibility: public opinion guide , social responsibility , self-discipline to bear the ethical responsibility and abide by relevant laws and regulations , accept the heteronomy , points out that the net-work media should strengthen self -discipline and self -reflection are scientific and objective , guide the netizens rational appeal , to assume the social ethical responsibility of building a harmonious doctor -patient relationship .

〔Abstract〕 By retrieving the literatures from the official website of ONC , Tongfang Knowledge Network and the Internet , the paper conducts a survey of the conformance test and certification of health information standards in America from certification subject , certifica-tion object, test method and process, certification standard, certification mode and process, etc.It aims to provide a reference for the conformance test of health information standard in China .