Atherosclerosis ; complications ; Fatty Liver ; complications ; Humans ; Non-alcoholic Fatty Liver Disease

Coronary Disease ; Humans ; Smoking

ObjectiveTo evaluate the effects of selective cyclooxygenase-2 inhibitor nimesulide lone and combined with cisplatin on tumor growth,and Ki67 and Caspase-3 expression in lung cancer xenografts in nude mice. Methods The mice were randomly divided into 4 groups:the control group,the nimesulide group,the cisplatin group and the nimesulide combined with cisplatin group. A549 cells were injected into BALB/c nude mice subcutaneously.On the 21st day after treatment tumor tissues were collected,and the xenografts growth were observed. The expression of Ki67 and Caspase-3 were detected by immunohistochemical method.ResultsNimesulide combined with cisplatin could significantly inhibited the xenografts growth compared with nimesulide or cisplatin.The tumor inhibition rate was 44.33％ in the nimesulide group,53.61％ in the cisplatin group and 80.41％ in the nimesulide combined with cisplatin group (P ＜ 0.05).Immunohistochemical analysis showed that the expression rates of Caspase-3 was significantly increased in the nimesulide combined with cisplatin group (67.43 ± 23.57 ) ％,the cisplatin group (48.40 ± 20.37 ) ％,and the nimesulide group (38.65 ± 15.37)％,compared with the control group (27.63 ± 13.03)％ (P ＜ 0.05).The expression rate of Caspase-3 was significantly increased in the nimesulide combined with cisplatin group compared with the nimesulide group or the cisplatin group (P ＜ 0.05 ).The expression rate of Ki67 was significantly decreased in the nimesulide group combined with cisplatin group (24.34 ± 15.90)％,the cisplatin group (40.85 ± 22.47)％ and the nimesulide group (53.33 ± 19.67)％ compared with the control group ( 80.43 ± 16.88 ) ％ ( P ＜ 0.05 ).The expression of Ki67 was significantly decreased in the nimesulide combined with cisplatin group compared with the nimesulide group or the cisplatin group ( P ＜ 0.05 ).Conclusion Nimesulide can inhibit human lung cancer A549 cells xenografts in nude mice growth,Nimesulide enhanced the inhibitory effects of cisplatin.The mechanism may be related to inhibition of tumor cell Ki-67 expression,increased expression of Caspase-3,inhibition of tumor cell proliferation,and inducing tumor cell apoptosis.

Objective To study the role of bacterial collagenase in wound healing with observing capillary neovascularization, epidermis regeneration and collagen fiber reparation in vivo. Method A total of 28 New Zealand rabbits were made into models of superficial soft tissue trauma and divided into two groups. In the experimental group, the right limbs of rabbits were traumatized intentionally in the same length and depth, and their wounds were treated with bacterial collagenase liquor 133 MIU/mL, and the wounds were sutured. Similarly, in the control group, the left limbs of the rabbits were modeled in the same way and their wounds were managed with physiological saline instead and closed. Their wounds were observed continually 1, 2, 3, 5, 7, 10 and 14 days after traumatized. Data were analyzed statistically by using One-way ANOVA, LSD-test, and Wilcoxon rank test. Results There were no significant differences in capillary neovascularization and epidermis regeneration between two groups ( F = 0.12, P = 0.740 and F = 0.95, P = 0.33, respectively), but significant difference in collagen fiber reparation was found between two groups (F = 6.63, P = 0.01). Conclusions In the progress of wound healing, bacterial collagenase can improve the collagen fiber reparation without effects on the new capillary vasculogenesis and epidermis regeneration. The bacterial collagenase does not play a positive role in wound healing.

The teaching material library of nutrition and food hygiene was established based on the concept of integrable ware.This paper discussed on the methods and the experiences.The establishing principle must be clear,knowledge points should be separated into parts accurately,courseware and other computerized teaching materials should be made into full use and convenient and efficient retrieval method must be established.In the future,teaching material library and platform construction should be improved and study of educational technology should be strengthened to improve teaching quality and adapt to modern teaching requirements.

OBJECTIVE:To investigate the characteristics of adverse drug reactions(ADR)of the hospitals(Level 2 and above) of Wuxi in order to promote rational use of drugs in the clinic.METHODS:2 473 cases of ADR collected from Jan.2005 to Dec.2006 in 11 hospitals(Level 2 and above)of Wuxi were retrospectively studied.RESULTS:Most patients who had ADR were old patients over 60 years and teenagers(≤10years).Most ADR were caused by anti-infection drugs(60.9%),followed by the preparations of Chinese materia medica(13.7%).The intravenous medication was the main route of medication resulting in serious adverse reactions;Most of the ADR cases were known and mild, 74 cases were severe and scarce(3.0%).CONCLU-SION:More attention should be paid to monitoring clinical ADR and the quality of ADR reports needs to be improved.

OBJECTIVE:To evaluate the cost-effectiveness of domestic versus imported acarbose tablets in the treatment of type 2 diabetic mellitus.METHODS:Based on the published medication data for type 2 diabetic mellitus,patients with type 2 diabetic mellitus were assigned to receive domestic acarbose tablets(Group A) or imported acarbose tablets(Group B).The cost-effectiveness of the two groups were compared applying the theory in pharmacoeconomics.RESULTS:Domestic and imported acarbose showed the same effectiveness for type 2 diabetic mellitus,while the costs in the two groups were significantly different.CONCLUSION:As compared with imported acarbose,the domestic acarbose has higher cost-effectiveness ratio.

Personnel resource is very important for the development of emergency department and emergency medicine.In recent years a series of researches for the standardized training of emergency residents have been progressed in the Emergency Depart- ment of West China Hospital.It goes through three phases.The idea of the training changes from“general physician”to“emer- gency specialist”.And the training is designed,practised and assessed with standardization.

From the third day after Wenchuan Earthquake,the Chinese government and army dispatched 90 helicopters per day to transport the wounded to Chengdu City (about 100 km away from earthquake-hit area). From 14 May to 21 May 2008, West China Hospital of Sichuan University received totally 760 wounded mainly from Wenchuan, Yingxiu, Maoxian, Beichuan, Lixian, Mianzu. Transfering patients by helicopter is characterized by rapid,flexible and non-restriction from terrain conditions.Compared with transfer on the land,injury induced during the transfer is reduced. However, the height of helicopter cabin is low,so it's difficult for doctors to carry out related treatment works. The instability during the flight also brought a lot of difficulties for the medical care.The rapid change of speed during take-off and landing caused a series of physiological changes on patients, such as thoracic pressure. Wenchuan is located in the mountains,and this also brought difficulties for the flight. The potentially savable victims,whose lives wouldn't be threatened with bad wound and wouldn't die in a short time,should be given the priority of transfer by helicopter. During the trip of transfer,the life-threatening occurrences, such as asphyxia, persistent convulsion and shock, were well prevented. At the same time, airway management, monitoring of vital signs and necessary consolation were important. After arrival at airport, the medical staff waiting at airport should watch and evaluate the patency of airway and vital signs carefully. If the airway obstruction, vital signs unstable and some other critical events are identified, the first aid at airport is essential. When patients are stable,they can be transported to the nearest hospital where the emergency treatment is available. Besides observation of patients' condition, registration of the name, age, address and the phone number of casualties should be done in the ambulance. Stick a label of the general information and the preliminary diagnosis of the patient on bare intact skin. The selection of casualties before transfer and the first aid at the airport are very important for successful short distance trip by helicopter.