COX-2 is the inducible form of cyclooxygenase,which is overexpressed in non-small cell lung cancer,especially in the adenocarcinoma,and is involved in the production of lung cancer drug resistance, reducing the sensitivities of tumor cells to chemotherapy drugs. COX-2 inhibitors have the effects of antitumor and prevention of tumor formation. Therefore,in order to reverse the drug resistance of tumor cells,improve the sur-vival rate and the prognosis of patients with tumor,the application of COX-2 inhibitors as adjuvant chemotherapy and combined with chemotherapy drugs has become a new direction in the treatment of lung cancer.

Objective To investigate the effects of muscle relaxants on motor evoked potentials (MEPs) monitoring during intracranial surgery. Methods 62 patients with neurological tumor were divided into 2 groups: muscle relaxant group (n=21) and non-muscle relaxant group (n=41). The incidence of successful MEPs monitoring was investigated. Results The incidence of successful MEPs monitoring was 76.2% in the muscle relaxant group and 41.5% in the non-muscle relaxant group (P<0.05). Conclusion Muscle relaxants can affect the MEP monitoring, which would not be administered as possible during anesthesia for intracranial surgery in functional area.

Objective: To observe polyethylenimine-mediated BMP-7 gene transfection in promoting fracture healing in elderly rats. Methods: Male SD rats (20-month-old) were randomly divided into 3 groups: group A received BMP-7 gene therapy mediated by polyehtylenimine, group B was treated with normal saline and group C was treated with BMP-7 plasmid without polyethylenimine. Right femoral shaft fracture model was established in all rats. Animals in group A received transdermal injection of pcDNA3. 1-BMP-7/PET; those in group B and C received the same volume of normal saline and pcDNA3. 1-BMP-7, respectively. X-ray photography, histological observation (H-E staining), immunohistochemical staining of collagen type I, biomechanical and bone mineral density test were employed to assess the healing of fracture 2, 4, and 8 weeks after treatment. Results: The results of X ray and H-E staining showed no fracture healing in the 3 groups during the 8th week after fracture; however, the growth of coloboma in group A was better than that of the other 2 groups, with partial bone union between the fracture ends. Staining of collagen type I showed deeper, wider staining in group A compared with the other 2 group. Anti-bending intensity and bone mineral density tests showed that the parameters in group A were better than those of the other 2 groups (P<0.05 or 0.01). Conclusion: Polyethylenimine-mediated hBMP-7 gene transfer can effectively promote fracture healing in elderly rats.

People- oriented is the purpose of modem medicine pattern.To realize the purpose,the medical worker must improve humanity accomplishment all round,morals - oriented,and serve for common people with humanity character of the nobleness and perfect medical treatment technology.

Objective To explore the changes of different echocardiographic indices in patients receiving catheter ablation for atrial fibrillation with different outcomes. Methods 75 patients received catheter ablation for atrial fibrillation during the period of January 2012 to February 2014. Before and 6 monthes after ablation , all the patients were examined left atrial volume index (LAVi), left atrium pressure (LAP), and left atrial ejection force (LAF) with echocardiography. Then after 6 months, according to the outcomes of the procedure, we divided the patients into two groups and compared the changes of these three parameters. Results In sinus group, LAVi decreased while LAF increased after treatment [LAVi: (29.3 ± 1.9) vs. (41.2 ± 9.3), LAF:(1.31 ± 0.61) (kg·cm)/s2 vs. (0.83 ± 0.22) (kg·cm)/s2, P < 0.05]; But LAP did not change [(55.7 ± 23.3)mmHg vs. (58.9 ± 18.6)mmHg, P > 0.05]; while in recurrent group, there were no changes in LAVi, LAP, and LAF after treatment (P > 0.05). Conclusions Radiofrequency catheter ablation is safe and effective in treatment of atrial fibrillation, resulting in significant improvements in left atrial volume and cardiac function.

2 in B27 positive groups and X = l. 58

Objective To evaluate the application of duct endoscopy in the diagnosis of breast diseases characterized by nipple discharge.Methods One hundred and sixty-nine consecutive cases from October 2003 to September 2004 who had duct endoscopy for nipple discharge were reviewed and followed up.Results Of 169 cases,79 ductitis,62 intraductal papilloma,9 intraductal carcinoma in situ,19 without breast diseases were diagnosed under duct endoscopy.During following up,the diagnostic accuracy of intraductal papilloma and intraductal carcinoma in situ with duct endoscopy was 89.8%(44/49) and 55.6%(5/9),respectively.There was only one patient who had breast pain and mild skin redness which was cured with oral antibiotics for 3 days.Conclusion Duct endoscopy is an only technique to explore the etiology for duct discharge under vision.It is direct,safe and effective,with a high accuracy.

Objective To establish a method for determination of 7 preservatives, benzyl alechol, sorbic acid, benzoic acid, methyl-paraben, ethyl-paraben, propyl-paraben, butyl-paraben, in cosmetics in one time by gas chromatography. Methods The cosmetic sample was dissolved by methanol, extracted by ultrasonic and separated by packed column of DB1, the optimized experimental condition was explored also. Results The concentrations of 7 preservatives showed a positive correlation with the peak heights at the following ranges, 100-500 ?g/ml for benzyl alechol, 200-600 ?g/ml for sorbic acid, 25-500 ?g/ml for benzoic acid, methyl-paraben, ethyl-paraben, propyl-paraben and butyl-paraben, the correlative coefficients were 0.998 3-0.999 9. The detection limits were 5.5, 57.1, 29.6, 6.2, 5.5, 6.2, 6.2 ng/ml respectively. The recovery rates were 91.6%-102.7%. RSDs were 2.2%-10.2%. Conclusion The present method can perfectly separate the seven preservatives in cosmetics mentioned above with some advantages, such as simple, accurate, sensitive and quick.

AIM To explore the effects of trip-tolide on endothelin-1 and its gene expression in lung of asthmatic guinea-pigs. METHODS The contents of ET-1 in lung of asthmatic guinea-pigs were measured with radioimmunoassay, and the expressions of ET-1, ETA, ETB and ECEmRNA with reverse transcription DNA polymerase chain reaction (RT-PCR). RESULTS 0 All of the indexes were increased after guinea-pigs were induced asthma. At the 4th hour after first induction of asthma, ET-1 reached the top, but at the 2rd hour, ET-1mRNA, ETAmRNA and ECEmRNA were markedly higher than control group (P

AIM: To explore the variation of blood biochemistry and arterial blood gas of patients with systemic inflammatory response syndrome (SIRS) in the early time after trauma and improve the diagnosis and first aid. METHODS: Eighty-eight patients with trauma from August 2003 to February 2004 were divided into two groups by their AIS-ISS90 score. The data of temperature, pulse, respiratory rate, white blood cell counts, Hb, blood glucose and arterial blood gas (PaO 2, PaCO 2, HCO 3 -, AG) were collected and compared with each group by statistic methods. RESULTS: Of the 88 patients, 49 underwent SIRS, 12 in light trauma group (ISS≥16) and 37 in severe trauma group (ISS45 mmHg or 0 05). 13 patients had MODS in severe trauma group and 2 died while none had MODS or died in light trauma group. CONCLUSION: Application of AIS-ISS90 and SIRS-related blood biochemistry and arterial blood gas is beneficial for the diagnosis and treatment for patients in the early time after trauma. [