The terahertz laboratory medicine (THz-LabMed) is an intergrated cross-frontier field which involves multi-disciplinary including medicine,biology,biomedical engineering,physics,optics,computer science,information and materials.Using THz technologies for label-free detection and analysis of biomedical macromolecules,cell and tissues,the THz-LabMed is also the core components of terahertz biomedine (THz-BioMed) which focuses on the biomedical application of THz technologies.THz label-free detection is being paid more and more attention because of its unique advantages worldwide and becomes a hot spot for the application of THz wave technology and methods in life science.Application of THz technology in biomedicine involves many fields globally,including disease diagnosis,recognition of protein status,label-free DNA sequencing,mechanism for absorption differences of biological tissue to THz wave,and radiation influence on biological samples and biological process.THz-LabMed is the global synchronized research in THz-BioMed field in China.It is important to seize the opportunities to develop new disciplines of laboratory medicine.

Breast cancer is the most common disease in women worldwide, which not only threatened the women’s survival time, but also inlfuenced their quality of life as well. Within this challenge, it’s important to optimize the current multidisciplinary treatment stratagem for breast cancer. Radiotherapy is one of the most important treatment modality for patients with breast cancer, with the trend to shrink the irradiated volume and shorten the total fraction times in recent years. Intraoperative radiation therapy (IORT) as a fast and convenient procedure has the ability to deliver a high, single-fraction radiation dose to tumor beds with minimal exposure of surrounding tissues (lung, heart, etc.), which could be displaced or shielded right after the tumor removal during the surgical procedure. Right now, IORT has been either integrated as a boost technique in multimodal approaches using postoperative EBRT in the treatment of early breast cancer patients undergoing breast conservation surgery or used as a single dose accelerated partial breast irradiation technique for these patients. This review discussed the rationale of IORT, the beneifts and limitations of IORT, the indication and the clinical results of this procedure, including treatment related side-effects as well in order to provide the preliminary evidence based approach for early breast cancer patients.

Egr-1 is an important transcription factor, which regulates at least 30 kinds of gene expression. Egr-1 couples extracellular signals to long-term responses by altering expression of Egr-1 target genes. So egr-1 can directly or indirectly affect cell differentiation,apoptosis,immune response,injury and repair. This article reviewed the progress in Egr-1 and the lung disease.

Objective To construct mouse granulocyte-macrophage colony stimulating factor (mGM-CSF) gene eukaryotic expressing plasmid pcDNA3-GM-CSF, to transfect the recombinant into erythroleukemia cell line FBL-3, and identify their biological activity.Methods GM-CSF gene eukaryotic expressing plasmid was constructed by subclone and recombinant was transfected into FBL-3 cells by electroporation. After screening by G418 and cloning by limiting dilution,we obtained positive cell clones(FBL-3-GM-CSF). PCR and RT-PCR were used to identify the integration and stable expression of GM-SF gene in FBL-3-GM-CSF cells. The biological activity was confirmed by the hematopoietic progenitor cell proliferative assay and hematopoietic progenitor cell colony formation assay. Results Mouse GM-CSF cDNA was amplified from the prokaryotic expressing plasmid PET-30a(+)-GM-CSF by PCR firstly and BamH Ⅰ and EcoRⅠrestriction sites were introduced. The inserted fragment was cut by BamH Ⅰ and EcoR Ⅰ digestion and ligated into pcDNA3 vector. The pcDNA3-GM-CSF eukaryotic expressing plasmid was constructed. The recombinant was cleared with appropriate endoneucleases and sequenced. The findings showed that the orientation of the insert was correct, while no rearrangement or mutation was found. PCR and RT-PCR assay showed that GM-CSF gene had integrated into FBL-3-GM-CSF cells and stably expressed. The hematopoietic progenitor cell proliferative assay and hematopoietic progenitor cell colony formation assay demonstrated that the cultured supernatant of FBL-3-GM-CSF cells of expressing GM-CSF should obviously stimulate proliferation of murine marrow mononuclear cells, and could stimulate hematopoietic progenitor cell colony formation. The number of colony formation was 54.67?4.83. The rate of colony formation was 0.547 %.Conclusions GM-CSF gene eukaryotic expressing plasmid is constructed successfully. A cell clone, which can express stably GM-CSF gene and possess biological activity,is obtained. Our studies have founded the base for the preparation of GM-CSF gene-modified vaccine of tumor cell and the study of feasibility of immune therapy of leukemia.

Humans ; Infant, Newborn ; Infant, Newborn, Diseases ; Lupus Erythematosus, Systemic

Adult ; Aged ; Aged, 80 and over ; Anemia ; chemically induced ; Antineoplastic Combined Chemotherapy Protocols ; adverse effects ; therapeutic use ; Carboplatin ; administration & dosage ; Carcinoma, Non-Small-Cell Lung ; drug therapy ; pathology ; Deoxycytidine ; administration & dosage ; analogs & derivatives ; Female ; Follow-Up Studies ; Humans ; Leukopenia ; chemically induced ; Lung Neoplasms ; drug therapy ; pathology ; Male ; Middle Aged ; Neoplasm Staging ; Paclitaxel ; administration & dosage ; Remission Induction ; Survival Rate ; Thrombocytopenia ; chemically induced

OBJECTIVE:To improve the quality standard for Ganmao shangfengke tea. METHODS:TLC was used to identify the Saposhnikovia divaricata and Glycyrrhizae Radix et Rhizoma in the preparation. HPLC was used to determine the content of magnolol and honokiol:the column was Phenomenex Luna C18 with mobile phase of methanol-water(77:23,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 294 nm,column temperature was room temperature,and the injection volume 5 μl. RE-SULTS:The TLC spots of S. divaricata and Glycyrrhizae Radix et Rhizoma were clear and well separated. The linear range was 3.13-100.2 μg(r=0.9999) for magnolol and 3.05-97.6 μg(r=0.9999) for honokiol;RSDs of precision,stability and accuracy tests were lower than 2%;recoveries were 98.2%-99.5%(RSD=0.5%,n=6)and 98.6%-99.6%(RSD=0.4%,n=6),respective-ly. CONCLUSIONS:The improved standard can be used for the quality control of Ganmao shangfengke tea.

Adult ; Female ; Humans ; Male ; Medical Staff, Hospital ; statistics & numerical data ; Middle Aged ; Nursing Staff, Hospital ; statistics & numerical data ; Sports ; statistics & numerical data

Hydrocarbons, Brominated ; metabolism ; toxicity ; Occupational Exposure