BACKGROUND:Anterior decompression, bone graft fusion, plate and screw fixation are commonly used in clinic for the treatment of thoracolumbar burst or comminuted fractures. The posterior surgery including decompression laminectomy and pedicle screw internal fixation.
OBJECTIVE:To explore the biomechanical changes of spine after thoracolumbar fracture and dislocation treated with pedicle screw internal fixation.
METHODS:Forty-six patients with thoracolumbar fracture and dislocation were treated with pedicle screw internal fixation, and then the recovery of spinal cord injury and fracture reduction were observed after internal fixation, immediately after internal fixation and 1-year fol ow-up.
RESULTS AND CONCLUSION:Al the 46 cases were fol owed-up. Frankel function classification assessment was used to evaluate the spinal cord injury grade. There was no significant difference in the percentage of A grade between periods (P>0.05), and there were significant differences in the percentage of B-E grades when compared between immediately postoperative period, 1-year fol ow-up period and preoperative period (P<0.05), but there was no significant difference when compared between immediately postoperative period and 1-year-fol ow period (P>0.05). The anterior height percentage, posterior height percentage and Cobb angle indicators were used to assess the fracture reduction, and the results showed there was no significant difference in posterior height between periods (P>0.05), and there was significant difference in Cobb angle when compared between immediately postoperative period, 1-year fol ow-up period and preoperative period (P<0.05), but there was no significant difference between immediately postoperative period and 1-year fol ow-up period (P>0.05). The results indicate that pedicle screw internal fixation system for the treatment of thoracolumbar spinal fracture and dislocation is conducive to the fracture reduction and functional recovery.

Majority of humans requiring radioprotection in medical center are healthy. The most important thing is consummate radioprotection and facilities for environmental protection, and scientific arrangement of staffs and rooms as well.

Positive ratio of chest X-ray is analysed to explore whether it is reasonable chest X-ray in annual physical examination.

Objective:To study the influences of astragulas(黄芪) on cerebroischemiareperf usion injury in gerbils.Methods:The cerebral tissue delayed neuron death(DND) models of gerbil were established by means of ligating bilateral carotid arteries for 15 minutes then reperfusing for 48 hours and divided into shamoperation(SO) group,ischemiareperfusion(IR) group and astragulas injection (AI，黄芪注射液) group.The activities of Na ＋K＋ATPase and nitric oxide synthase(NOS) and the changes in contents of nitric oxide(NO) and excitat ory amino acid(EAA) in cerebral tissue were compared in different groups.Results:(1)In comparison with SO group the activities of Na＋K＋ATPase in IR and AI group were obviously decreased furthermore the decreases in IR group were mo re significant than those in AI group(all P<0.01).(2) The changes in co nten ts of lactic acid(LD) in cerebral tissue in all the groups had not statistically significance.(3)In comparison with SO group the activitis of NOS and the conten ts of NO in IR and AI group were reduced but the reductions in IR group were muc h more significant (P<0.05).(4)The contents of glutamic acid(Glu) in IR and AI group were higher than those in SO group and the increases in IR group w .05),meanwhile they in IR group were higher than those in AI group but the di fference was not significant.(6)Among all the groups the contents of γam inobutyric a cid (γGABA) and glycine(Gly) had not significant differences (all P>0.05).Conclusions:The mechanisms of astragulas on anticerebral ischemiareperfusion inj ury may be related to its preventing the reductions of activities of Na＋K ＋ATPase,NO S,and the decreases in synthetic volume of NO as well as its heightening the con tents of EAA after ischemia of brain tissue.

Objective: To investigate the feasibility and operative effect of the one-stage microsurgery for clipping of bilateral posterior communicating artery aneurysms (BpcoAA). Methods: The clinical data of 28 patients with BpcoAA were analyzed retrospectively. All patients underwent craniotomy and microsurgical clipping of BPcoAA via unilateral pterional approach. The patients were followed up for 6 months to 3 years after microsurgery. The head 3 D-CTA of the patients and their general conditions were reexamined. The Glasgow outcome scale (COS) scores were used to assess the prognosis. Results: Circled digit oneGood preoperative 3D-CTA showed 28 cases with 56 aneurysms, the aneurysms were clipped completely in 24 cases, the contralateral aneurysms were not clipped completely in 3 cases, the aneurysm was not clipped completely on the approach side in 1 case, and none of the patients died. The aneurysms ruptured in 8 cases on the approach sides during the microsurgery. Circled digit twoAfter microsurgery, 7 cases had hydrocephalus, and 5 had vasospasm on the approach sides and 3 on the contralateral sides, 3 suffered pulmonary infection, and 2 had oculomotor nerve injury on the contralateral sides. Circled digit threeThe mean follow-up time of the patients was 1.7 years. The GOS scores: 5 points in 10 cases, 4 points in 7 cases, 3 points in 9 cases, and 2 points in 2 cases. There were no aneurysm recurrence and new aneurysm formation. The patients whose aneurysms were not clipped completely had no recurrence of bleeding. Conclusion: According to 3D-CTA examination, using unilateral pterional approach, aiming at the location of the optic chiasm and the contralateral aneurysm pointing, the one-stage microsurgery for clipping of BpcoAA is safe and feasible.

Objectives Research into effect on PBL model in prosthodontics education.Methods The PBL model was performed in education of a part of prosthodontics,and education effect was evaluated by table analysis.Results PBL model is proved to be better than the multimedia education model in the training of students'self-study ability,learning initiative,problem-analyzing and problem-solving competence,and the team spirit etc.Conclution Comparaed with the multimedia education model,PBL model is of significance in some respects.

Objective To investigate the expression of HPV-DNA and p16,Ki-67 proteins in intraepithelial lesion of cervix (CIN) so as to provide the aluable evidence for the early clinical diagnosis and genetic therapy of the CIN.Methods Hybrid capture2 was used to detect HPV DNA in 35 cases of CIN Ⅰ,44 cases of CIN Ⅱ,and 20 cases of CIN Ⅲ.Immunohistochemical MaxVision was used to research the expression of p16 and Ki-67 protein in CIN Ⅰ,CIN Ⅱ and CIN Ⅲ.The results were analyzed with clinicopathological factors.Results Positive expression rates of HPV-DNA were 31.4 ％ (11/35),63.6 ％ (28/44),85.0 ％ (17/20) in CIN Ⅰ,CIN Ⅱ and CIN Ⅲ,respectively.The expression rates of p16 in the three groups were 65.7 ％ (23/35),97.7 ％ (43/44),100.0 ％ (20/20),there were statistically significant difference (x2 =21.30,P =0.00).The expression rates of Ki-67 in the three groups were 77.1％ (27/35),79.5 ％ (35/44),95.0 ％ (19/20),there were no difference (x2 =3.00,P =0.22).Conclusions HPV infection is closely related to the occurrence and development of CIN.p16,Ki-67 can be used as objective marks in diagnosis of CIN.

Objective To investigate the relative factors affecting long-term prognosis of visual acuity in diabetic patients after implantation of artificial lens. Methods A total of 86 eyes of 86 diabetic patients with implantation of artificial lens were enrolled in this study. The gender, age, core rigidity, course of disease, preoperative levels of blood sugar and HbA1c, insulin administration, anterior chamber inflammation at the first day after operation, hypertension, and hyperlipemia were recorded. Multi-factor relation analysis of visual acuity was performed at the first month and the third year after operation, respectively. Results The postoperative visual acuity at the first week after operation was related to the preoperative level of blood sugar and HbA1c, core rigidity, anterior chamber inflammation at the first day after operation and types of operation(P

Objective To explore the new preservation method of cornea by evaluating the structure and function of rabbit's endothelia on the condition of whole-eye preservation with aqueous removed and recipient's serum tamponaded. Methods Forty New Zealand big white rabbits (80 eyes) were random divided into two groups, 40 eyes in group A (control group) and 40 eyes in group B (experimental group). The vitality of endothelial cell on the condition of tow corneal preservation methods which were the moist chamber preservation (group A) and the whole-eye preservation with aqueous removed and recipient's serum tamponaded (group B) was compared. At the 2nd day, the 5th day, the 7th day, the 10th day, the vitality of endothelial cell was appraised through ultra-microstructure by scanning electron microscope and the trpan biuealizarin red stain. The corneal thickness was measured, and corneal endothelial cells density was calculated, and cell size was observed by image analysis system. Result In group B, the corneas remained transparent for 7 days , and the rate of vitality was 90% for7 days and that was over 80% for 10 days. In group A, the corneas remained transparent for 2 days, and the endothelial cell losing and dying were found after 5 days. Cell vitality, cell density and cell size had no statistical difference between Group B for 7 days and group A for 2 days. (all P＞0. 05). In group A, at the 5th day, the 7th day ,the 10th day, the average corneal thickness were (0.64 ± 0.04) mm, (0. 79 ± 0. 03) mm , (1.06 ± 0. 03) mm. In the group B, at the 5th day, the 7th day ,the l0th day, the average corneal thickness were (0. 55 ±0.03)mm, (0.65 ±0. 02) mm , (0. 85 ± 0. 05) mm. The average corneal thickness had significant difference between group A and group B (all P ＜ 0. 05). Conclusion Recipient serum had the function with supporting the structure and function of rabbit's corneal endothelial cell, and it could prolong the storage time with the moist chamberstorage at the same time.

1.0 mmol/L).The clinical side effects after medication circa were compared between two groups.Results There was no significant difference in myelosuppression,liver functional lesion,gastrointestinal tract reaction and infection in 2 groups.But following the increase of MTX blood drug level,the incidence rate of skin mucosa contamination,electrocardiographic abnormality,the cardiac creatase abnormity and nervous system symptoms significantly increased.Conclusions In the course of child ALL treatment with HD-MTX+CF,the side effects are common and individual difference is obvious.Specific treatment on individuals is suggested.J Appl Clin Pediatr,2006,21(3):170-171