BACKGROUND: Impoverished undergraduate is a new and special group appearing under the market economy. Strengthening depth and extent is necessary to probe into and study it.OBJECTIVE: To investigate the social support of impoverished undergraduates and probe into its effect on mental health of impoverished undergraduates.DESIGN: Cross-section survey, clustering stratified sampling SETTING: College of Education of Jiangxi Normal University PARTICIPANTS: Totally 445 impoverished undergraduates of Jiangxi Normal University, Nanchang University, Jiangxi Medical College, East China Jiaotong University and so on of nanchang city were recruited in October 2002. All the participants were volunteers.METHODS: Social Support Rating Scale (SSRS) was used to evaluate the social support of impoverished undergraduates. The scale is composed of 10 items including objective support, subjective support, and utilization degree of support. Symptom Checklist 90 (SCL-90) was used to evaluate the mental health status of impoverished undergraduates. SCL-90 has 90 tests, using 1 to 5 degree to score( from not present to severe), including somatization, obsessive compulsive, interpersonal sensitivity, depression,anxiety, hostility, phobic anxiety, paranoid ideation, psychoticism (9 factors). During the process of investigation, the testee was tested singly in 2 hours without interference and influence of others. The questionnaires were taken back and the authenticity and validity of the questionnaires was verified.RESULTS: All the 500 questionnaires handed out were taken back. Totally 445 pieces of effective questionnaires were involved in the analysis with the effective rate of 89%. ① There was significant difference in objective support and utilization degree of the support between the impoverished undergraduate of either gender (P ＜ 0.01 or P ＜ 0.05); ②There were significant differences in subjective support and utilization degree of support between only-child and non-only-child (P ＜ 0.01 or P ＜ 0.05);③ There was significant difference in utilization degree of the support between impoverished undergraduates from city and impoverished undergraduates from country(P ＜ 0.01 or P ＜ 0.05 );④Obvious correlation existed between mental health and social support of the impoverished undergraduates;⑤Through regression analysis, it showed that social support had significant effect on the mental health of impoverished undergraduate (F= 12.770,P=0.000).CONCLUSION: Significant difference existed between different genders,only-child or non-only-child, impoverished undergraduates coming from urban area or rural area. Social support has significant effect on the mental health of impoverished undergraduates.

Influenza A virus is an enveloped virus that belongs to the Orthomyxoviridae family. It has 8 negative RNA segments that encode 16 viral proteins. The viral polymerase consists of 3 proteins (PB 1, PB2 and PA) which plays an important role in the transcription and replication of the influenza A virus. Polymerase basic protein 1 (PB 1) is a critical member of viral polymerase complex. In order to further study the function of PB1, we need to prepare the PB1 antibody with good quality. Therefore, we amplified PB1 conserved region (nt1648-2265) by PCR and cloned it into pET-30a vector, and transformed into Escherichia coli BL2 1. The expression of His tagged PB 1 protein was induced by IPTG, and His-PB 1 proteins were purified by Ni-NTA resin. For preparation of PB 1 protein antiserum, rabbits were immunized with His-PB 1 fusion protein 3 times. Then the titer of PB 1 polyclonal antibody was measured by indirect ELISA. The antibody was purified by membrane affinity purification and subjected to immunoblotting analysis. Data showed that PB1 antibody can recognize PB 1 protein from WSN virus infected or pCMV FLAG-PB 1 transfected cells. Meanwhile, PB 1 antibody can also recognize specifically other subtype strains of influenza A virus such as H9N2 and H3N2. PB 1 polyclonal antibody we generated will be a useful tool to study the biological function of PB1.
Animals ; Antibodies, Viral ; biosynthesis ; Cloning, Molecular ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli ; metabolism ; Genetic Vectors ; Influenza A Virus, H3N2 Subtype ; Influenza A Virus, H9N2 Subtype ; Plasmids ; Rabbits ; Viral Proteins ; immunology

Objective To explore the significance of Survivin and PTEN in gastric cancer development and correlation between Survivin and PTEN.Methods Immunohistochemical methods were applied to detect the expres.sion of Survivin and PTEN in 105 pathological specimens of gastric cancer, in comparison to that in 98 negative gastric mucosa tissues.Results The positive rate of Survivin expression was 82.9% (87/105) in tissue specimens of gastric cancer and 6.1% (6/98) in tissue specimens of non-gastric cancer;The difference between them was statistically sig nificant (P <0.01).The positive rates of PTEN expression were 34.3% (36/105) in tissue specimens of gastric canc-er and 99.0% (97/98) in tissue specimens of non-gastric cancer;The difference between them was statistically signifi-cant ( P <0.01).The expression of Survivin and PTEN in tissues of gastric cancer had nothing to do with the gender of patients ( P > 0.05 ) , but was correlated with the degree of tumor cell differentiation, depth of invasion, lymph node me-tastasis, distant organ metastasis and TNM staging(P <0.05).Analysis by Spearman showed that Survivin was nega-tively correlated with PTEN( r = -0.23 ,P < 0.05 ).Conclusion The incidence of gastric cancer was related to the abnormally high level of Survivin expression and absence of PTEN expression;The expression of Survivin and PTEN in tissues of gastric cancer was unrelated with the gender of patients, but related to the degree of tumor cell differentia-tion, depth of invasion,lymph node metastasis,distant organ metastasis and TNM staging.The expression of Survivin was negatively correlated with the expression of PTEN.

Cardia ; Gastric Fundus ; Humans ; Stomach Neoplasms ; surgery

To introduce the experiences of nursing risk management in Children's Cancer Center in Hongkong from aspects of organization structure and responsibility,clinical application of the risk management measures so to give reference to the hospital's nursing risk management in our country.

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is one of the most important pathogen and can cause infection ranging from superficial skin infections to life-threatening disease. The prevalence of CA-MRSA is rising in children in these years. Therefore, the review will cover the research progress in the CA-MRSA prevalence, risk factors, clinical manifestation, treatment and preventions in children. The risk factors and disease patterns and antimicrobial resistant rates in different parts of China in the recent decade will be also discussed.

Objective To explore the clinical nursing of hip developmental dislocation in infants with Ferguson operation.Method The nursing strategy was performed including observation of the surgical incision,skin care,care of cast and instructions to their parents. Result No pressure sores occurred in all the 34 cases of hip developmental dislocation.1 case had delayed allergy reaction because of use of contrast media during operation,1 cast syndrome,and 19 edema of perineum of different degrees including 6 severe edemata,which were cured using wet compress with Adlerika.Conclusion It is of great importance for the reduced incurrence of operation complications and promising operative effect to observe the conditions and treat timely problems in postoperative nursing.

OBJECTIVE:To investigate the effect of Saccharomyces boulardii adjuvant with aluminium phosphate on symp-toms and signs disappearance time,immune function and inflammatory factors in children with acute diarrhea. METHODS:Totally 110 children with acute diarrhea were chosen from our hospital during Mar. 2013-Mar. 2016,and then randomly divided into con-trol group and observation group according to random number table,with 55 cases in each group. Control group was given Alumini-um phosphate gel 2.5 g,po,qd,on the basis of conventional treatment. Observation group was additionally given S. boulardii cap-sules 0.25 g,po,bid,on the basis of control group. Both groups were treated for 3 d. Clinical efficacies,symptoms and signs(di-arrhea,abdominal pain,vomiting,abnormal stool routine)disappearance time as well as stool frequency,stool character scores, immune function indexes(CD3+,CD4+,CD8+,CD4+/CD8+),inflammatory factors(IL-6,IL-10,TNF-α)before and after treat-ment,the occurrence of ADR were compared between 2 groups. RESULTS:The total response rate of observation group (90.91%)was significantly higher than that of control group(70.91%);the symptoms and signs disappearance time was signifi-cantly shorter than control group,with statistical significance(P<0.05). Before treatment,there was no statistical significance in stool frequency,stool character scores,immune function indexes or inflammatory factor levels between 2 groups(P>0.05). After treatment,stool frequency,stool character scores and inflammatory factor levels of 2 groups were significantly lower than before, and the observation group was significantly lower than the control group;the levels of CD3+,CD4+,CD4+/CD8+ were significantly higher than before treatment and control group,and the level of CD8 + was significantly lower than before treatment and control group,with statistical significance(P<0.05). There was no significant difference in immune function indexes of control group be-fore and after treatment(P>0.05). Total incidence of dry mouth and constipation was 5.45% in control group and 9.09% in obser-vation group,without statistical significance(P>0.05). CONCLUSIONS:S. boulardii adjuvant with aluminium phosphate in the treatment of pediatric acute diarrhea can efficiently improve symptoms and signs,shorten disease duration,improve immune func-tion and reduce inflammatory reaction with good safety.

Objective To investigate the regulative effect of the extract of Radix Ranunculi Ternati,Radix Sophorae Flavescenti,Prunella vulgaris L.and Stellera chamaejasme L.on cellular immunity induced by multiple drugs resistant bacillus tuberculosis(MDR-TB) from pneumoconiosis patients complicated with tuberculosis in rats.Methods MDR-TB model in rats was induced by MDR-TB.Normal control group were feed by standard feed,model group were irrigated by normal saline,and the other groups were respectively feed by the extract of Chinese herbal medicines.The content of IFN-γ,IL-4,IL-10 and IL-12 were examined by ELISA.RT-PCR was used to detect the mRNA levels of them.Results The content of IFN-γof the four extracts of Chinese herbal medicines were (2.01 ±0.73 ),( 1.92±0.56),( 1.98 ±0.67 ) and (1.94±0.59) pg/ml,IL-4 were (6.01±1.46),(6.12±1.35),(6.47±1.46) and (6.15±1.44) pg/ml,IL-10 were (12.09±3.07),( 12.45±4.01 ),( 12.13±3.43) and (12.54±3.78) pg/ml,IL-12 were (2.99±0.89),(2.75±0.84),(3.02±0.86) and (2.89±0.75) pg/ml.Compared to the model group,they resulted in significant in serum IFN-γ,IL-12,IL-4 and IL-10 (P＜0.05 or P＜0.01 ),the mRNA levels of them were significantly (P＜0.05 or P＜0.01 ).Conclusion The four extracts of Chinese herbal medicines can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription,accordingly provide the theory base of healing of pneumoconiosis patients complicated with tuberculosis with them.