After a description of the differences between formative evaluation and summative evaluation, the appli-cation of formative evaluation in literature retrieval course evaluation in medical colleges and universities was elabo-rated by analyzing the current situation of literature retrieval course and its evaluation in medical colleges and uni-versities with Xinjiang Medical University as an example, which showed that the principles should be followed when formative evaluation is applied in literature retrieval course evaluation.The problems related with the application of formative evaluation in literature retrieval course evaluation were pointed out.

Objective To explore the relationships between serum insulin-like growth factor 1 (IGF-1) level and children with tourette syndrome, and the significance of IGF-1 level for the diagnosis of tourette syndrome.Methods Enzyme-linked immunosorbent assay (ELISA) was adopted to detect the serum level of IGF-1 and S100B of 53 children patients with tourette syndrome and 53 children with physical examination.The receiver operating characteristic (ROC) curves were used to determine the cut-off value and assess the sensitivity,specificity and diagnostic efficiency.Results The concentration of IGF-1 was significantly lower in the children with tourette syndrome group than in normal group ((41.51±4.23) μg/L vs.(69.04±5.26) μg/ L,t=29.693,P＜0.01),and the concentration of S100B was significantly higher in the children with tourette syndrome group than in normal group((129.48±9.54) ng/L vs.(81.37±8.42) ng/L,t =27.526,P＜0.01) .When 59.28 and 93.35 ng/mL ware used as cut-off value, the sensitivity, specificity and area under curve for IGF-1 in diagnosis of children with tourette syndrome were 89.6％, 96.4％ and 0.931, respectively.The sensitivity,specificity and area under curve for S100B in diagnosis of children with tourette syndrome were 94.1％, 91.3％ and 0.969, respectively.And the diagnostic efficiency of IGF-1 was similar to that of S100B.Conclusion IGF-1 may play an important role in the development of tourette syndrome.Detection of IGF-1 in the serum is helpful in the diagnosis of children with tourette syndrome.

Objective To evaluate the effect of therapeutic communication on perioperative anxiety of cancer patients by using the Meta-analysis.Methods The randomized controlled studies of therapeutic communication for perioperative anxiety of cancer patients were gathered from Pubmed,Embase,Cochrane Library,China Biology Medicine Disc (CBM),China Scientific Journal Database by VIP (CSJD),China National Knowledge Infrastructure (CNKI) and Wangfang Data,while other relative researches were also searched by hand.According to inclusion and exclusion criteria articles were evaluated by two reviewers independently.The studies were assessed according to the Juni assessment.RevMan 5.2 was used for statistical analysis.Results Seven studies with 506 patients were included,of which therapeutic intervention group contained 256 patients,the control group included 250 patients.Meta-analysis showed that compared with the control group,therapeutic communication system could reduce perioperative anxiety of cancer patients [MD=-8.00,95％CI (-8.91--7.10),P ＜0.01],improve positive perioperative coping of patients [MD=5.50,95％CI(4.05-6.95),P ＜ 0.01],reduce negative perioperative coping of patients [MD=-7.62,95％CI (-9.13--6.11),P ＜ 0.01],increase the satisfaction degree of patients [MD=0.55,95％CI (0.33-0.77),P ＜0.01],the differenccs were statistically significant.Conclusions Therapeutic communication can contribute to relieve perioperative anxiety of cancer patients,optimize their coping styles and improve the satisfaction degree of patients with nursing work.

Recent evidence has shown that neurogenesis occurs throughout adulthood, and neural stem cells reside in the adult central nervous system (CNS) in mammals. Experimental stroke in adult mammals increases neurogenesis from neural stem cells or progenitor cells located in the dentate subgranular zone and the subventricular zone lining the lateral ventricle. New neurons can migrate to the areas of damage regions and express morphological markers characteristic of died neurons. These findings bring hope for self-repair after brain injury. The author of this paper reviewed the adult neurogenesis and its regulation in vivo, and described evidence for stroke-induced neurogenesis and neuronal replacement in the adult, and discussed the future research directions about neurogenesis after stroke and other brain injuries.

Objective To observe the effect of Ginkgo on IL-4,IL-5 in serum of asthmatic mice. Methods 40 mouse were randomly divided into three groups(control group, sensitization group, and therapeutic group). The mouse asthmatic models were established by sensitizing with aerosolized ovalbumin(OVA). IL-4,IL-5 in serum in 16 sensitization mouse and 8 control mouse were measured by ELISA, respectively. We injected 0.15ml Ginkgo solution in the abdominal cavity of 16 therapeutic mouse before the episode of asthma. After that, they were challenged with aerosolized OVA and IL-4,IL-5 in serum were measured by ELISA. Results The levels of IL-4,IL-5 in serum in control group were (12.965?2.450)pg/ml, (13.398?4.460)pg/ml, respectively, (16.889?4.835)pg/ml, (19.471?7.207)pg/ml in sensitization, (13.695?4.068)pg/ml, (14.774?5.749)pg/ml in therapeutic group. In the sensitization group, the levels of IL-4,IL-5 in serum were higher than those of the control group( P

OBJECTIVE:To investigate inhibitory effects of matrine on the proliferation of human bladder cancer BIU-87 cells and its mechanism. METHODS:The cell viability was detected by MTT assay and inhibitory rate of cell proliferation was calculat-ed after human bladder cancer BIU-87 cells were treated with 0(negative control),0.5,1.0,2.0 and 4.0 mg/ml matrine for 24,48 and 72 h,respectively. After treated with 0 (negative control),0.5,1.0,2.0 and 4.0 mg/ml matrine for 48 h,the cell cycle and apoptotic rate were detected by flow cytometry;the expression of Survivin,Caspase-3 and Caspase-7 protein were detected by Western blot assay. RESULTS:Compared with negative control,the proliferation of BIU-87 cells were significantly inhibited after incubated with 1.0-4.0 mg/ml matrine for 24,48 and 72 h(P<0.05 or P<0.01),and inhibitory rate of cell proliferation increased in concentration and time-dependant manner;after treated for 48 h,the percentage of G0/G1 phase cells and apoptotic rate in-creased,while the percentage of cells at S phase and G2/M phase were decreased (P<0.05 or P<0.01);the expression of Cas-pase-3 and Caspase-7 protein increased,while the expression of survivin protein decreased after incubated with 0.5-4.0 mg/ml ma-trine for 48 h. CONCLUSIONS:Matrine can inhibit the proliferation of human bladder cancer BIU-87 cells,block the cell cycle and induce apoptosis;its mechanism may be related to the expression regulation of Survivin,Caspase-3 and Caspase-7.

Objective:To explore the significance of combined fibrinogen concentration and neutrophil-lymphocyte ratio as a prognostic marker of colorectal cancer.Methods:A retrospective analysis of the hospital in June 2010-June 2011 of received and accepted the surgical treatment of colorectal cancer patients,respectively analysis the relationship NLR and FIB with the pathological characteristics of colorectal cancer,neutrophils and lymphocytes ratio (NLR) and the concentration of fibrinogen (FIB) were combined to form a index (FIB-NLR).250 patients with colorectal cancer recording the scores were divided into three groups,patients NLR acuity 2.95 and FIB≥348 mg/dl as FIB-NLR 2 groups,NLR acuity 2.95 and FIB < 348 mg/dl or NLR < 2.95 and FIB acuity 348 mg/dl for 1 group,NLR < 2.95 and FIB < 348 mg/dl of 0 group,and analyzed three groups with invasion depth,clinical staging and lymph node metastasis of colorectal cancer,nerve invasion,distant metastasis and histological grades.And the three groups respectively for survival analysis,and carried on the comparison to the survival rates of three groups.Results:Moderate and advanced and with lymph node metastasis of colorectal cancer patients was significantly higher than the earlier stage and no lymph node metastasis in patients with NLR,statistically significant difference (P<0.001);tumor infiltration depth deeper,nerve invasion and distant metastasis of the NLR value was significantly higher in patients with infiltrating depth shallow,without nerve invasion and distant metastasis in patients with NLR values,the difference was statistically significant (P=0.006,P=0.002,P=0.007).Moderate and advanced stage,lymph node metastasis and distant metastasis of colorectal cancer patients with the FIB values were significantly higher than the early and without lymph node metastasis and distant metastasis of colorectal cancer patients FIB values,the difference was statistically significant (P<0.001),the deeper the infiltration depth and colorectal cancer patients with neural infiltrates FIB values significantly higher than the infiltration and shallow depth,of FIB values for patients with nerve infiltration,the difference was statistically significant (P=0.015,P=0.012).NLR and FIB in tumor histologic stage,age,gender,tumor location,had no significant association (P>0.05). The patients with later clinical stage of colorectal cancer,the deeper the infiltration depth,lymph node metastasis,distant metastasis had a high FIB-NLR score than infiltration and shallow depth,without lymph node metastasis and distant metastasis,no nerve infiltration patients was high,the difference was statistically significant (P<0.001).Survival analysis found that the higher the score of patients the 5-year survival rate was lower,the difference had statistical significance (P=0.001).Conclusion:Neutrophil-lymphocytes ratio (NLR) and fibrinogen (FIB) joint form a index FIB-NLR may be a potential progression effective index which estimate the progression of colorectal cancer.

Background The visual evoked potential (VEP) is an important functional index to assess visual cortex plasticity.Monocular form deprivation(MD) of rat is a classic model to study visual cortex plasticity change.Utilizing VEP technique record the shift of ocular dominance in adult rats model is of an important significance for explore the treating opportunities of amblyopia.Objective The present study was to observe the pattern VEP changes in different ages of Long-Evens rats and the adult rats after binocular form deprivation.Methods Thirty-six healthy SPF Long-Evens rats were divided into PW3,PW4,PW5,PW6 groups according to the postnatal weeks,and PW7 rats included 36 SPF Long-Evens rats.The left eye lids of the rats were sutured in PW3,PW6 and PW7 rats for 3,5,7 days respectively,and PVEP in both left and right eye were recorded to assess the rat age that visual cortex plasticity ended.Bilateral PVEP were recorded in PW7 rats,and the bilateral eyelids were sutured to establish the bilateral form deprivation models.The right eyes were opened in 7,10,14 days,and the left eyes were opened in the next 3,5,7 days respectively and the PVEPs were recorded again to find the shift of ocular dominance and whether binocular form deprivation induce the visual cortex plasticity in adult rats.Results The P100amplitudes of the left eyes were gradually declined and those of the right eyes were raised from 3 to 7 days after MD of the left eyes in rats of PW3 group in comparison with before MD ( P＜0.01 ).In PW6 groups,no significant changes in P100amplitudes of both the right and left eyes were found in the third day after MD,but significant raise in the right eyes and lowing in the left eyes were seen in the 5 and 7 days after MD in comparison with before MD(P＜0.01 ).No any P100changes in both eyes were found in 3-7 days after MD compared with before MD in PW7 rats (P＞0.05).The C/I ratio (contralateral VEP amplitude to ipsilateral VEP amplitude in occluded eyes) in the rats of PW3,PW4 and PW5 groups were 1.07±0.15,1.16±0.16 and 1.14±0.15 respectively in 3 days after MD,showing the significant lowing in comparison with before MD (2.69±0.45,2.58±0.4,2.62±0.32) (P＜0.01),but those of PW6 and PW7 were unchanged (2.80±0.48 vs 2.90±0.46,2.59 ±0.36 vs 2.90±0.46,P＞0.05 ),indicating the absence of ocular dominance plasticity in the adult rats.In rats of PW7 with binocular form deprivation for 14 days,a significant decrease was observed in the C/I ratio in rats with next MD for 3 days,demonstrating that the visual cortex plasticity was reactivated after 14 days of binocular form deprivation in PW7 rats( 1.33±0.18 vs 2.70±0.45,P＜0.01 ).Conclusions PVEP can be recorded in Long-Evens rats.It is a major index for identifying the shift of ocular dominance in the Long-Evens rats.Binocular form deprivation can reflect the visual cortex plasticity in the adult rats.

After the significance to asses the value of information resources in hospital library was elaborated, the methods to assess the value of information resources in hospital library were constructed by contingent valuation and investment return analysis in combination with the practical service in hospital library according to the analysis of the economic value assessment in domestic and foreign public libraries and academic libraries.

Objective To investigate the influence of knocking down Ezrin expression in combination with HSP70-induced immune killing on the apoptosis and proliferation of mouse osteosarcoma cells.Methods Human osteosarcoma cell line MG63 was cultured,the HSP70 and Ezrin-shRNA DNA fragments were cloned into the expression vector pGFP-V-RS containing CMV and pU6 promoters,and constructed the expression vector pGFP-V-RS-shRNA and pGFP-V-RS-shRNA-HSP70.The vectors were transfected into MG63 cell line,respectively.The status of transfected MG63 cells was observed by fluorescent microscope.The expressions of Ezrin and HSP70 were examined by real time RT-PCR and Western blot.Flow cytometry 、MTS test was used to detect the changes of cell apoptosis and proliferation,and changes of the expression of apoptosis and cell cycle related proteins was detected by Western blot.The specific cytotoxic T lymphocytes (CTLs) were induced by HSP70,and its killing effect on target MG63 tumor cells was analyzed by MTT assay.Results The specific vector simultaneously knocking down Ezrin and overexpressing HSP70 was constructed for the first time in China and confirmed by fluorescence microscope and gene/protein expression analysis.Compared to Ezrin knock-down alone,simultaneous HSP70 overexpression partially recovered the promoted cellular apoptosis and proliferation suppression by Ezrin knock-down,however,when compared to the normal control,the apoptosis rate of LM 8 cells was still significantly increased from 11.01％±0.22％ to 24.28％±0.50％,while the proliferation rate decreased from 395.14％±2.24％ to 310％± 2.83％.In addition,Western detected that Ezrin-shRNA could promote the expression of Bax.However,the expression of Ezrin-shRNA could reduce the Bcl-2 and Cyclin D1.Ezrin-shRNA/HSP70 also has the same effect.MTT assays revealed that the CTL cytotoxic effect on target MG63 tumor cells at all concentrations were significantly higher in CT+IL-2+HSP70 group compared with that in CT+ IL-2 group,with a cytotoxicity as high as 56.33％± 1.95％.Conclusion Simultaneous knocking down Ezrin and overexpressing HSP70 promotes the apoptosis and inhibits the proliferation of osteosarcoma cell.And the HSP70 can induce CTL which enhances the killing effect on tumor cell.And the HSP70 can induce CTL which enhance the killing effect on tumor cell.