Protein phosphatase 2A(PP2A)is a major Ser/Thr phosphatase involved in sevelral cellular signal transduction pathways.The reversible phosphorylation of proteins is accomplished by opposing activities of kinases and phosphatases.PP2A controls the specific dephosphorylation of thousands of phosphoprotein substrates and thus regulates physiological and biochemical processes of organism.The changes of the physiological activities of PP2A closely relate to apoptosis and regeneration of injured intestinal epithelial cell,and PP2A may be a regulatory factor in balancing the apoptosis and regeneration of intestinal epithehal cell.

Objective To investigate prolactin(PRL)levels in patient with hyperprolactinemia in different period of time. Methods Used electrochemical luminescence method,Roche E170 automatic immune analyzer determination of detect 124 cases female patients of hyperprolactinemia (PRL>880 uIU/ml)at 7:30AM,10:00AM and 4:00PM respectivelye of plas-ma PRL level,and in accordance with the polyethylene glycol (PEG)6000 precipitate PRL after A PRL recovery rate of into macroprolactin (MPRL)group (A PRL recovery rate of≤40%),single PRL group (A PRL recovery rate of> 60%),sus-picious PRL group (40%

Objective To explore the value of the multi-phasic spiral CT scan in the diagnosis of gallbladder carcinoma.Methods The characteristic CT findings were reviewed in 42 cases with gallbladder carcinoma proven pathologically.Results The gall bladder carcinoma had certain enhancing characteristics: high density in hepatic artery period,same or high density in porch vein period,and no clear fadeaway in delaying period.In other words,the gall bladder carcinoma was higher enhancement than normal gallbladder wall and liver,and had long-term enhancement.Conclusion The multi-phasic spiral CT scan plays an important role in diagnosis and differential diagnosis of gallbladder carcinoma.

Background Gene transfection is an effective therapeutic avenue to target many kinds of eye diseases.Non-viral vectors with high transfection efficiency,long-term expression,low toxicity and high expression levels are pivotal in gene therapy of corneal disease.Objective This study was to evaluate and compare the safety and efficiency between EntransterTM and liposome vectors for transfer of CD25 siRNA in rat cornea.Methods Eighty male SPF SD rats were randomly divided into EntransterTM-CD25 siRNA group,liposome-CD25 siRNA group,simple CD25 siRNA group and normal saline solution (NSS) group with the right eye as experimental eyes.Corneal epithelia of the rats were completely removed after ocular surficial anesthesia,and 50 μl EntransterTM-CD25 siRNA,liposome-CD25 siRNA,CD25 siRNA solution and NSS were topical administered in the eyes respectively.Ocular response and green fluorescence number on the corneas were examined under the slit lamp assisted microscope 12 hours,24 hours,3 days and 7 days after use of the drugs.The rats were sacrificed and the corneas were obtained,and corneal histopathological examination was performed by using hematoxylin eosin stain.The gene transferred efficiency in the corneas was evaluated by fluorescence technology,and the safety of EntransterTM and liposome carriers was assessed using TUNEL stain.The expression and location of CD11b in the corneas were detected by immunofluorescence technology.The use and care of the experimental animals complied with Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Committee.Results The quantity and intensity of fluorescence staining in the corneas were significantly increased in the EntransterTMCD25 siRNA group in comparison with the liposome-CD25 siRNA group,and the corneal fluorescence appeared earlier in the simple CD25 siRNA group,but it disappeared in 24 hours after transfection.Corneal histopathological examination revealed that the corneal edema and inflammatory cell infiltration in corneal epithelium after gene transfection were more dominant in the liposome-CD25 siRNA group than those in the EntransterTM-CD25 siRNA group,simple CD25 siRNA group and NSS group,and no abnormality was seen in the stroma and endothelium.The number of inflammatory cells was more in the liposome-CD25 siRNA group than that in the EntransterTM-CD25 siRNA group,simple CD25 siRNA group and NSS group (all at P =0.00).The number of apoptosis cells was significantly more in the liposome-CD25 siRNA group than that in the EntransterTM-CD25 siRNA group,simple CD25 siRNA group and NSS group in 12 hours and 3 days after transfection (all at P =0.00).Immunofluorescence assay showed the expression of CD11b primarily located in the corneal epithelial and stromal layers.The expression of CD11b was gradually enhanced over time in the liposome-CD25 siRNA group and peaked in 24 hours after transfection.However,the expression was absent in the EntransterTM-CD25 siRNA group,simple CD25 siRNA group and NSS group.Conclusions EntransterTM nanometer material-mediated transfection of CD25 siRNA in corneas of normal SD rats appears to have high transfection efficiency,low toxicity and slight irritating response to corneas,and EntransterTM vector is currently available for the gene therapy of corneal disease.

Objective To investigate the calcification of thyroid nodules detected by ultrasound and its relation with thyroid carcinoma.Methods Four thousand one hundred and eighty-six cases of thyroid disease were surgically treated during a period of five years.Retrospective analysis was made to compare preoperative color Doppler ultrasound and postoperative pathology.Results The incidence of calcification,non-microcalcification,and microcalcification were significantly higher in thyroid carcinoma than that in benign diseases(P＜0.05).For predicting thyroid cancer,microcalcification was more significant than non-microcalcification(P＜0.01).There was a significant difierence in the microcalcification between the different age groups(2.4%for patients of 45 and older,16.8%for patients younger than 45;P＜0.05):There was no difference in the calcification between male and female groups(P＞0.05);There was a significant difierence in malignant calcified nodules between difierent nodule groups(70.7%for patients of solitat'y.49.1%for patients of muhiple:P＜0.05).Conclusion Microcalcifications in the thyroid is related to thyroid cancer.especially to micro-papillary carcinoma.

OBJECTIVE To investigate the risk factors of infection after incisional wound in colon cancer patients for better prevention and treatment. METHODS To retrospectively analyze the colon cancer patients in whom the incision infection happened from Jan 2002 to Apr 2008. RESULTS The incidence of postoperative wound infections of the patients was 2.7%. In univariate analysis,the difference whether infections were happened in patients with diabetes was significant(P

Objective To investigate the protective effect of edaravone on ischemia reperfusion injury(IRI) in rat liver.Methods Sixty rats were randomly divided into experimental group and controll groups(30 in each) after establishing animal liver IRI model with partial reperfusion injury under normal temperature.Just after initiation of reperfusion and 1h later,edaravone was administered in the experiment group,and the same volume of normal saline was administered in the control group.The lipid peroxidation(LPO) hepatic enzymes and the level of TNF-? mRNA and E-selectin mRNA in plasma were measured at 0,2,4 h after initiation of reperfusion.We also serially quantified hepatic expression of mRNAs for TNF-? and E-selectin with RT-PCR.Results In the experiment group,hepatic LPO and hepatic enzyme were significantly less than that in the saline group(P

Objective To explore the changes of plasma catecholamines(CA)[including epinephrine(E) and norepinephrine(NE)] and atrial natriuretic peptide(ANP) in children with varying degrees of left ventricular ejection function(LVEF) in chronic heart failure(CHF),and establish natriuretic hormone factors as one of the diagnostic criteria of CHF.Methods Thirty-five patients with CHF and 35 children in good health were enrolled,and the concentration of plasma NE,E,ANP and the LVEF were determined.Results 1.The levels of plasma NE,E and ANP in 35 patients with CHF before treatment were significantly higher than those in control group(Pa

Biochemistry and molecular biology is one of the most important professional basic courses for students in medical universities.Constructing scientific teaching quality control system for biochemistry and molecular biology is essential for improving its teaching quality.This paper discussed on some principles needed to be followed in constructing the teaching quality control system for biochemistry and molecular biology in medical universities and elucidated the three leveled frame of this system including university,college and department.This paper also illuminated on the main contents,implementation,organization system,evaluation system and incentive measures of this teaching quality control system.

Emergency departments of hospitals are exposed to risks of patient complaints and disputes, and the emergency department of a stomatology hospital is no exception. Medical disputes at the emergency department of Peking University Stomatology Hospital, as registered at the department of medical affairs of the hospital in the past ten years, are analyzed with statistical processing. The results are used in studying characteristics of medical disputes frequently found in stomatology emergency cases, for references and recommendations on prevention and avoidance of such disputes in future.