Objectives: To evaluate the effects of glutamine on the nutritional metabolism and permeability of intestinal mucosa in MODS patients.Methods: The randomized and controlled study was designed.Twenty MODS patients were randomly divided into control group and treatment group.The concentration of serum albumin,transferrin and blood sugar,L(lactulose) and M(mannitol) ratio in urine,nitrogen balance,the related complication were compared and obserwed.Results: In treatment group,the concentration of serum albumin,prealbumin and transferrin were higher than that of control group on the same time and showed significant difference(P

Objective: To construct and characterize GFP-mfgl 2 fusion protein expression plasmid (pEGFP-mfgl 2) and provide a direct and simplified methodology for primary assessment of the effect of mfgl 2 siRNA on the mfgl 2 gene expression. Methods: mfgl 2 cDNA was amplified from the mfgl 2 cDNA library pBluescript-m166 (pm166) of mouse genomic P1 plasmid and recloned into pEGFP-N2 upstream of GFP gene. The pEGFP-mfgl 2 was analyzed by restriction endonucleases BamH I and Hind III to ensure the orientation and the sequence. This fusion plasmid was then transfected into CHO cells and the fusion protein expression was observed by fluorescent microscope. Rusults: A 1.3 kb long cDNA was obtained. Restriction endonucleases and sequencing assays showed the correct orientation and sequence. After 24-48 hours transfection in CHO cells, the expression of pEGFP-mfgl 2 can be visualized through fluorescent microscope. Conclusion: pEGFP mfgl 2 has been constructed successfully. The recombinant vector can express GFP-mfgl 2 fusion protein. It provides a direct and simplified methodology for primary assessment of the effect of mfgl 2 siRNA on the mfgl 2 gene expression.

Objective To investigate the influence of inspired oxygen fraction (FiO2) on the ratio of PaO2/FiO2(P/F) during the implementation of lung protective ventilation strategy in patients with acute respiratory distress syndrome(ARDS) in order to unravel its clinical significance. Method This was a prospective study of 16 selected patients with ARDS treated with mechanical ventilation ( MV ) to get ratio of P/F in range of 100 to 200 by PEEP≥5 cmH2O and high inspired oxygen. After lung recruitment maneuvers by BiPAP with high pressure (PH) of 40 cmH2O for40 s, the MV was maintained the basic requirement for stabilizing the patients for 30 minutes. A series of FiO2 were set at fractions of 0.5,0.6,0.7,0.8,0.9 and 1in random sequence, and the changes of respiratory mechanics, blood gas and hemodynamics under the different concentrations of FiO2 were analyzed by using SPSS version 13.0 software. Results ( 1 ) The ratio of P/F increased as FiO2 increased, and it's significant as FiO2 increased to 0.7 or above. As the fractions of FiO2 were set at 0.5 and 1. O, the ratios of P/F changed in 24.70% ± 23.36% respectively. ( 2 ) Of them,6 patients ( 37.5% ) treated with FiO2 set at 0.5 had the ratio of P/F ＜ 200, and the fraction of FiO2 was increased to 1.0, the P/F ＞ 200. (3) FiO2 and Qs/Qt were negatively correlated ( r = - 0.390, P = O. 027 ),the higher inspired oxygen fraction, the lower shunt. When the fractions of FiO2 were set at 0.5 and 1.0 ,there was a positive correlation between △Qs/Qt and △P/F( r = 0.82, P = 0.005 ). Conclusions The inspired oxygen fraction affects the ratio of P/F, which may be resulted from shunt and it may influence the diagnosis of ARDS.

Objective To investigate the plasma expression of plasma prekallikrein(KLKB1)in latent autoimmune diabetes in a‐dults(LADA),type1diabetes(T1DM),type2diabetes(T2DM)andhealthypeople,anditsrelationshipwithLADAincombination with other indicators .Methods Among the four groups ,KLKB1 ,glycosylated hemoglobin(HbA1c) ,fasting blood glucose(FPG) ,2 h postprandial plasma glucose(2 h PG) ,Fasting c‐peptide(FCP) ,2 h postprandial C peptide(2 h CP) ,and glutamic acid decarboxy‐lase antibody(GADA)were detected respectively .And the detection results were analyzed by statistics .Results By comparison , there were statistically significant difference between LADA group and other groups on FPG(except for T2DM group) ,2 h PG , HbA1c ,FCP and 2 h CP(P< 0 .05) .And except for T1DM group ,there was statistically significant difference between LADA group and other groups on GADA ,too(P<0 .05) .The plasma expression of KLKB1 in LADA was significantly higher than those in T2DM group and NC group(P<0 .05) .The levels of KLKB1 was related with FCP、HbA1c、FPG、2 h PG(P<0 .05) ,and it was not related with age ,course ,and 2 h CP(P>0 .05) .Receiver operating characteristic (ROC) showed that only using KLKB1 to di‐agnose LADA had its limitation .Conclusion KLKB1 could be used as a clinical indicator to predict the onset of LADA to a certain degree .We could screen for LADA by using KLKB1 and other indicators in people at high risk .

Acupuncture Therapy ; Defecation ; Female ; Humans ; Ileus ; physiopathology ; therapy ; Middle Aged

Abstract?AIM: To discuss clinical efficacy and safety of 10g/L atropine with short covering for children with amblyopia.?METHODS: Eighty -eight children ( 88 eyes ) with amblyopia, staying in hospital from February 2011 to February 2014 for treatment, were divided into control group ( n =44 ) and observation group ( n =44 ) . The control group only given short covering therapy was observed.Observation group was given 10g/L atropine treatment besides covering.Clinical efficacy, treatment compliance, visual acuity, corrected spherical degree of amblyopia eye and adverse events were observed and compared.?RESULTS:1) After treatment, total effective rate of the observation group was 95% ( 42/44 ) , significantly higher than that of control group ( 80%, 35/44, P<0.05 ); 2 ) excellent compliance rate of the observation group was 95% ( 42/44 ) , significantly higher than that of control group (82%, 36/44, P<0.05);3) visual acuity of the two groups when the disease was first diagnosed was not significantly different (P>0.05), but increased number of lines of vision and corrected spherical degree of amblyopia eye in the observation group were significantly higher (P<0.05);4) in the observation group total rate of adverse events was 9% ( 4/44 ) , significantly lower than that in the control group (23%, 10/44, P<0.05).?CONCLUSION: The combined therapy, 1% atropine with short covering, is effective and safe for amblyopia in children.

Key trial activities include: development of the trial protocol;development of standard operating procedures;development of support systems and tools;generation and approval of trial information documents;selection of trial sites and the selection of properly qualified,trained,and experienced investigators and study personnel;ethics committee review and approval of the protocol;review and approval by applicable regulatory authorities;enrollment of subjects into the study: recruitment,eligibility,and informed consent;the investigational product(s): quality,handling,and accounting;trial data acquisition: conducting the trial;trial data acquisition: conducting the trial; safety management and reporting;monitoring the trial;managing trial data;quality assurance of the trial performance and data;reporting the trial.

Objective To compare the safety of two antibody inductors, namely lymphocytes scavenger and IL-2 receptor blocking agent, in living kidney transplantation. Methods The data of 191 patients, who received living kidney transplant in our hospital from Feb. 2007 to Jul. 2012, were retrospectively analyzed, and grouped according to the inductors they received as: a) lymphocytes scavenger group (n=56), with rabbit antithymocyte immunoglobulin (rATG, 4 cases) and porcine antihuman T-lymphocyte immunoglobulin (pATG, 52 cases) served as the inductor; b) IL-2 receptor blocking agent group (n=54), with basiliximab (40 cases) and daclizumab (14 cases) served as the inductor; and c) control group (n=8l). The incidence of rejection and infection, and the survival rate of patient/allograft within one year were then compared among the three groups. Results Within one year after the transplantation, the incidence of acute rejection in lymphocytes scavenger group, IL-2 receptor blocking agent group and control group was 12.5%, 11.1% and 28.4%, respectively. There was a significant difference between the two inductor groups and control group (P=0.003), but no significant difference was found between the two inductor groups (P>0.05). The incidence of delayed graft function (DGF) in the three groups was 8.9%, 7.4% and 13.6%, respectively, with no statistical significance (P>0.05). Also there was no significant difference among the three groups in the incidence of infection and the survival rate of patient/allograft within one year after transplantation (P>0.05). Conclusion Both inductors may significantly reduce the incidence of acute rejection within one year without increasing the incidence of infection and other adverse events, nor affect the postoperative patient/graft survival, so they are both safe and effective.

AIM: To explore curative effect of different drugs in treatment of senile wet macular degeneration.METHODS: We selected 98 patients 98 eyes with senile wet macular degeneration from July 2014 to January 2016 in our hospital as the research subjects.They were divided into control group and research group as the administration sequence, 49 patients in each group.Research group was treated with ranibizumab.The control group was treated with Conbercept.Both once per month and for 3mo.RESULTS: Uncorrected visual acuity, central macular retinal thickness and area of choroidal neovascularization (CNV) leakage before treatment of the two groups were not statistically different (P>0.05).At 1, 3 and 6mo after treatment, the uncorrected visual acuity was improved significantly, the central macular retinal thickness decreased significantly, and the area of CNV leakage decreased significantly (P<0.05).The differences on uncorrected visual acuity at 1mo after treatment, central macular retinal thickness and area of CNV were statistically significant (P<0.05), while those indexes at 3 and 6mo after treatment was not significant (P>0.05).In the follow up period, there was no severe complications in the two groups, such as persistent high intraocular pressure, retinal detachment or tear, endophthalmitis, or other systemic complications.There were subconjunctival hemorrhage in 10 eyes in research group, 8 eyes in control group, all of which recovered within 15d after treatment.Transient elevated intraocular pressure occurred in 7 eyes in research group, in 9 eyes in control group.The complication rates of the two groups were not significant (P>0.05).CONCLUSION: In the clinical treatment of senile patients with wet macular degeneration, treatment effect of Conbercept is not obvious at the early stage, but the effect is equivalent later and more economical.

Objective To observe the expression of triggering receptor-1 on myeloid cells (TREM-1) of mice with acute lung injury (ALI) in oder to find out its regularity and significance in inflammatory response of or-ganisms. Method Thirty BALB/C mice were randomly(random number) divided into normal control group (n =6) and ALl group (n = 24). The models of ALI were made with intraperitonal injection of lipopolysaccharide (LPS) in dose of 10 mg/kg. Specimens from peripheral blood and lung tissue were collected 6 h, 12 h, 24 h and 48 h after LPS injected. The fluorescent real-time quantitative reverse transcriptiun-polymerase chain (RT-PCR) was used to detect TREM-1 mRNA, and ELISA was employed for detection of TREM-1 protein and TNF-α protein, and HE staining was made doe the pathological Smith lung score under light microscope. Analysis of variance was used for comparison of TREM-1 mRNA, TNF-α and Smith lung injury score between two groups. Spearman corre-lation analysis was made to find out the relationship among these three variables. Results The expressions of TREM-1 mRNA in lung tissue of ALI mice 6 h, 12 h, 24 h, and 48 hours after injection of LPS were 6.61±0.08,34.71±0.83, 61.85±14.05 and 56.46±8.89, respectively which were higher than that in control group (1.00±0.00, P = 0.017, 0.009, 0.002 and 0.003, respectively). The expressions of TREM-1 mRNA in blood were 14.01±3.24, 47.07±0.98, 8.18±0.43 and 8.06±0.05, respectively which were higher than that in normal control group (1.00±0.00, P = 0.010, 0.004, 0.011 and 0.011, respectively). The expression of TREM-1 rnRNA in tissue began to increase 6 hours after modeling and reached its peak 24 hours later, and expres-sion of TREM-1 mRNA in blood reached its peak after 12 hours. The levels of TREM-1 protein in lung tissue of ALl mice 6 h,12 h,24 h and 48 hours after LPS injected were 997.8±114.62, 1579.70±45.92, 1123.9±108.2 and 429.8±89.96 pg/mL, respectively which were higher than that of mice in control group (279.22±4.62 pg/mL, P = 0.024, 0.007, 0.011 and 0.04, respectively). The level of TREM- 1 protein reached the peak 12 hours after LPS injected, but it had no significant correlation with the expression of TREM-1 mRNA (P =0.14). The levels of TNF-α protein in lung tissue of ALI mice 6 h, 12 h, 24 h and 48 hours after LPS injection were 313.16±39.50, 491.91±96.65, 388.48±29.84 and 282.5±52.76 pg/mL, respectively which were sig-nificantly higher than that of mice in control group (256.6±28.31 pg,/mL, P = 0.037, 0.019, 0.032 and 0.043, respectively). The TNF-α concentration was positively correlated with TREM-1 levels in lung tissue and with Smith pathological score (r = 0.795, P = 0.001: r = 0.499, P = 0.034), but not with the expression of TREM-1 mRNA (P = 0.176). Conclusions The expression of TREM-1 mRNA in lung tissue of mice with ALI is elevated, and the expression of TREM-1 mRNA is related to the level of TNF-α and the severity of the ALI in in-flammatory responses in lung. The expressions of TREM- 1 gene are not consistent with the levels of TREM- 1 pro-tein, suggesting another new functional proteins involved in immune regulation.