Objective To explore correlation between gene polymorphism of TNF-alpha and thyroid associated ophtalmopa thy (TAO) in Zhuang ethnic population.Methods DNA and serum samples were collected in patients with TAO in Han/Zhuang population.ELISA was applied to detect level of TNF-alpha in serum and PCR was applied to detect polymorphism of TNF-alpha gene 863C_A.Results The levels of TNF-alpha in patients with TAO increased significantly compared with control group (P ＜ 0.05),and more significantly in Zhuang population compared with Han population (P ＜ 0.05) ;gene frequency of CA was significantly higher in patients with TAO in Zhuang population than that in Han population (P ＜ 0.05),while gene frequency of AA was significantly lower (x2 =0.770,2.145,all P ＜ 0.05).Conclusion Serum level of TNF-alpha increases during occurrence of TAO,and the differences of expression level between different nationalities are due to gene polymorphism of TNF-alpha.

OBJECTIVE:To investigate the status quo and problems of the construction of pharmacy staff team from Hubei Wuling mountainous area,and to provide reference for the formulation of construction plan of pharmacy staff team. METHODS:Using questionnaire survey and telephone interviews,the pharmacy staff of 22 hospitals at second level or above from Hubei Wul-ing mountainous area during 2011-2014 were investigated in terms of number,age,educational background,professional title,etc. RESULTS & CONCLUSIONS:The shortage of pharmacy staff is seious in 22 hospitals at second level or above from this areas (open 10 606 beds,should be staffed 956 pharmacy personnel,actual number of pharmacy personnel is 502);the age structure is unreasonable,and only 220 pharmacy staff aged under 35 year-old;highly educated pharmacy staff accounted for low proportion, and bachelor degree or above took up 29.5%;pharmacy staff with professional title took up low proportion,and pharmacy staff with high,middle and junior professional titles accounted for 1:5:4. The situation of pharmacy staff in the region can be improved through following measures,including striving for policy recommendations,continuously enriching the quantity of pharmacy staff, integrating scientifically and building reasonable talent tem gradually,introducing talents,optimizing education structure of pharma-cy staff,strengthening the training,improving operation level of pharmacy staff,etc.

The structure, pharmaceutical functions and extraction and isolation methods of chlorgenic acid were reviewed to provide references for the research of chlorogenic acids and the production of the medicines chlorogenic acids.

Objective: To establish the determination of imperatorin and isoimperatorin in Tongketing Capsule (Radix Angelicae Dahuricae, Pheretima, etc.) by HPLC. Methods: The determination was carried out by HPLC. The mobile phase was acetonitrile water (60∶40) and UV detection wavelength was set at 245nm . The flow rate was 1.0mL?min -1 . The column temperature was 40℃ . Results: There were the good linear relationship between the absorption area value and the concentration in the range of 65?g～325?g for imperatorin and in the range of 50?g～250?g for isoimperatorin, respectively. The average recoveries were 98.3% ( RSD = 1.73% ) and 98.7% ( RSD = 1.65% ), respectively. Conclusion: The method is simple, accurate and can be used to determine imperatorin and isoimperatorin in Tongketing Capsule.

Enterovirus 71 (EV71) is one of the primary causative agents of hand, foot and mouth disease in children and closely associated with severe neurological complications and even deaths.EV71 outbreaks have occurred throughout the Asia-Pacific region since 1990s, posing global public health threat;however, no specific therapeutic strategy exists for severe EV71 infection.Several inactivated vaccine products have entered or finished the clinical trial stage, and some novel vaccine candidates, including live attenuated, subunit, and virus-like particle, show great potential for further develop-ment.This review summarizes the present situation and progress in the development of EV71 vaccines.

BACKGROUND: 6-minute walking test (6MWT) is a submaximal test, characterizing by easily processing and low cost, thus it is widely used in the clinic. However, how to change walking distance into maximal motor capacity needs to be further studied.OBJECTIVE: To study the correlation of 6-minute walking distance (D), work (W), peak oxygen uptake (VO_2 peak) and maximal oxygen uptake (VO_2 max) detected by Bruce.DESIGN, TIME AND SETTING: This study was performed at tshe Department of Rehabilitation Medicine, Zhongda Hospital affiliated to Southeast University from March to May 2009.PARTICIPANTS: A total of 25 healthy volunteers were enrolled, including 14 males and 11 females, with a mean age of (22.0±2.3) years.METHODS: All 25 subjects were tested with cardiopulmonary exercise testing (CPET) according to Bruce, VO_2 max and anaerobic threshold (AT) was detected respectively, then 6MWT" was adopted to detect 6-minute walking distance (D), work (W) and maximal oxygen uptake (VO_2 max) of subjects. Data of pulmonary gas exchange in breathing was detected by wireless remote sensor K4b~2 to obtain VO_2 max and VO_2 peak.MAIN OUTCOME MEASURES: ① Regularity of cardiac rate, oxygen uptake and respiratory frequency varied with time passing; ② Walking distance, work, oxygen uptake, cardiac rate and respiratory frequency were compared before and after testing; ③ Maximal oxygen uptake and anaerobic threshold with CPET were compared to peal oxygen uptake with 6MWT; ④ Correlation of distance, work between maximal oxygen uptake and peal oxygen uptake.RESULTS: There was no significant difference between AT from CPET and VO2 peak from 6MWT (P > 0.05). 6-minute walking distance was not correlated with either VO_2 peak or VO_2 max. However, 6-minute walking work was linearly correlated with both VO2 peak (r=0.779 7, P < 0.001) and VO_2 max (r=0.894 1, P < 0.001).CONCLUSION: 6-minute walking test is an exercise test at level of anaerobic threshold. 6-minute walking work can reflect both submaximal exercise capacity and maximal exercise capacity.

By reviewing its experimental studies as well as clinical investigations,experiences and proven cases,this paper presented the main directions and methods in the study of Guo-Min (GM) decoction.It proved that GM decoction had identified anti-inflammatory and anti-allergic effects.Its effect may be through blocking H1 receptors,and lowering IgE in serum of type I allergic reaction to achieve.It showed that the clinical observation on multi-center,large sample randomized double-blinded controlled trials combined with compatibility laws of Chinese herbs and prescriptions by chemomics will be the future study direction of GM decoction.It played significant role in the prevention and treatment of allergic diseases.

AIM: To investigate the role of SMADs singal pathway in rat myocardial hypertrophy. METHODS: The rat model of myocardial hypertrophy was produced by constriction of the abdominal aorta. The wet left vertricular/body weight ratio (LVMI) was measured. The expression of TGF-beta l and Smad 2, 3, 7 mRNA were assessed by RT-PCR. RESULTS: The LVMI and the expression of TGF-beta l and Smad 2, 3, 7 mRNA in cardiomyothy were increased in 3 day after the operation and continued at last 4 weeks. The peak expression of TGF-beta l and Smad 2, 3, 7 mRNA was in 2 weeks after operation. The expression of Smad 7 was increased in 3 days after operation, but the peak was in 1 week after operation, then decreased. CONCLUSION: The signal protein Smad 2, 3, 7 are involved in the progress of rat myocardial hypertrophy produced by constriction of abdominal aorta. [

Objective To investigate morbidity and analyze high risk factors of nosocomial infection in patients who underwent orthotopic liver transplantation (OLT) in our ICU, in order to draft possible preventive strategies. Methods The clinical data of 56 OLT patients from Apr 2002 to Dec 2004 were analyzed. Results Significantly high APACHE Ⅱ scores and total plasma bilirubin, low platelet count were found in patients with nosocomial infection. The number of patients who had received an additional operation and broad-spectrum antibiotics before OLT were greater in nosocomial infection (NI) group than in none-nosocomial infection (NNI) group. Notably, the longer the broad-spectrum antibiotics used before OLT, the higher the morbidity rate of nosocomial infection. No significant difference was found in age, Child-Pugh grade and plasma albumin level between the two groups. In addition, APACHE Ⅱ scores and total bilirubin level were significantly higher in patients acquired enterococcal infection than in those infected with other pathogenic microorganisms. Conclusions High APACHE Ⅱ score, high total bilirubin level, low platelet count and length of use of broad-spectrum antibiotics before OLT were closely associated with nosocomial infection. As an important indicator, change in total bilirubin level not only represented the liver function ,but also was one of the high risk factors to nosocomial infection in patients undergoing OLT.

Objective To evaluate the effect of catheterization on the serum prostate specific antigen (PSA) in acute urinary retention (AUR) patients due to benign prostate hyperplasia (BPH). Methods The data of 35 patients with AUR due to BPH were analyzed, and the changes in serum PSA values were compared before and after catheterization. The effect of AUR on serum PSA in BPH patients was discussed. Results It has been found that serum PSA declined from 9.93?8.85ng/ml to 6.46?5.75ng/ml after catheterization for the patients of AUR with BPH, and the values showed a significant difference (P0.05) in the patients in whom the serum PSA ≤4ng/ml, while a significant difference existed in both the group of patients in whom the serum PSA was 4~10ng/ml (from 5.95?1.43ng/ml to 4.09?2.00ng/ml, P10ng/ml (from 18.93?8.49ng/ml to 11.49?6.37ng/ml, P4ng/ml.