Magnetic resonance imaging (MRI),a commonly used imaging technique,has been extensively investigated in lung cancer diagnosis and staging.Many studies have demonstrated that MRI can be used for the differential diagnosis of benign and malignant pulmonary nodules and for the screening of lung cancer; it also has important value in TNM staging of non-small cell lung cancer (NSCLC),with equivalent sensitivity and specificity to 18F-fluorodeoxyglucose (18F-FDG) PET-CT,which suggests that MRI can be used as an alternative imaging modality in noninvasive diagnosis and staging of NSCLC.

Objective:To evaluate the etiology,elinic characteristics,diagnosis and treatments of spontaneous perforation of the bile duct in children. Methods:The clinical data of eight children with spontaneous perforation of the bile duct between 1998 -2006 were analyzed retrospectively. Results:5 of 8 cases were diagnosed bile duct perforativn before operation,all the patients recovered favorably after T-tube and pelvic drainage. 3 children complicated with choledochal cyst were cured afterⅡstage radical operation. Conclusions:the etiology of spontaneous perforation of the bile duct in children is unclear, panereaticobiliary confluence malfunction (PBCM) maybe the main reason. Peritoneocentesis is very important to the diagnosis, which is difficult in child preoperatively. The effective surgical treatments are T-tube and pelvic drainage. The children complicated with PBCM needⅡstage radical operation,and long term follow up are necessary for the children present with spontaneous perforation of the bile duct without PBCM.

Objective:To investigate the effect of ultrasound on the ultrastructure and expression of P-gp of Neuroblastoma cell in order to discuss the mechanisms of ultrasound affecting the chemotherapeutic sensitivity of SK-N-SH cell. Methods:The SK-N-SH cells were divided into the experimental group and the control group. In the experimental group,the cell suspensions were exposed to ultrasound irradiation. In the control group,the cell suspensions were exposed to sham irradiation . The changes of ultrastructure of tumor cells were observed by scanning electron microscope and the expression of P-gp in two groups were detected. Results:(1) In the pictures took by scanning electron microscope,we found that: the configuration of tumor cell changed and there were some holes of different diameters on the cell membrane and the numbers of microvillus reduced or disappeared after ultrasound irradiation.(2) The result of immunocytochemisty showed the expression rate of P-gp in SK-N-SH cells in the control group was 56.23%?9.86% vs 34.86%?6.19% in the experimental group (P

Objective To summarize the perioperative nursing experience in nursing children with formaral angulation deformity treated with sreminobicular crib external fixator. Method The clinical data of 15 children with formaral angulation deformity treated with sreminobicular crib external fixator were reviewed. Results All 15 children got recovered, with the femora extended by 4~9 cm. After operation, all of them were affected by knee flexion dysfunction and foot drop to varied extents, which were improved satisfactorily after systematic training. Conclusions The systematic and progressive training can prevent knee flexion dysfunction and foot drop. Careful observation and effective nursing play an important role in the treatment of formaral angulation deformity.

Meckel′s diverticulum is the most common congenital anomaly of the gastrointestinal tract. Most ca-ses are asymptomatic,while it can be symptomatic when complications happened,its complications often expressed as hematochezia,abdominal pain,vomit,fever,and so on. When signs or symptoms arise from a Meckel′s diverticulum, morbidity and mortality will increase. So make a definite diagnosis of the diverticulum play an important role in the treatment of Meckel′s diverticulum. In this essay,now illustrate the meaning of imaging examination about the diagnosis of Meckel′s diverticulum,wish to make a diagnosis as soon as possible.

Objective To study the ultrasonic characteristics of fetal cutaneous hemangioma and the association with perinatal outcomes.Methods Five fetuses with cutaneous hemangioma were detected by gray-scale and color Doppler ultrasound,compared with the result of pathology and hematology examinations after birth.Results Of the 5 cases diagnosed by ultrasound,3 had arteriovenous fistulas.In these 3 cases two fetuses developed high-output cardiac failure,and one developed cardiac insufficiency and thrombocytopenia.Finally one fetus was induced,one fetus died in the uterus and one neonate survived.The other two fetuses who had small tumors diagnosed by ultrasound did not develop any perinatal complications, and the neonates had favourable prognosis.Conclusions Massive hemangiomas are frequently associated with life-threatening complications such as high-output heart failure,thrombocytopenia and disseminated intravascular coagulopathy(Kasabach-Merritt syndrome).

Objective:To study the sensitivity and correlation between ANA,ENA and anti ds-DNA antibody in the patients with AID.Methods:Clinical data of patients with AID received treatment at our hospital from February 2013 to January 2014 was retro-spectively analyzed.The antibody expressions of ENA, ANA, anti ds-DNA antibody of the patients were detected.The autoantibody positive differences between the males and females were compared.The positive situation of ENA correspond to different fluorescent patterns of ANA and the correlation between the expression of ANA and the expressions of ENA,anti ds-DNA antibody were both dis-cussed.The relationship between the expression of ANA and the expression of ENA,dsDNA was also discussed.Results:A total of 180 patients were retrospective analyzed,including 67 males and 113 females.The positive rates of ANA,anti ds-DNA antibody in females were obviously higher than those in males.The rate of particle type ANA in the positive specimens of nRNP,ss-A was the highest.The rate of homogeneous type ANA of the positive specimens of Scl-70 was the highest.The granular pattern of the positive specimens of nRNP,ss-A had the highest rate in ANA.The homogeneous pattern of the positive specimens of Scl-70 had the highest rate in ANA.The positive rates of ENA, anti ds-DNA antibody in the positive specimens of ANA were obviously higher than those in the negative specimens of ANA.Conclusion:The positive rate of autoantibody in males was obviously lower than that in females.The particle type occupied higher rate in the karyotype of ANA.There was relationship between the positive detection rate of anti ds-DNA antibody,ENA and the positive rate of ANA.

Objective To investigate the effects of Gynostemma Pentaphyllum on serum markers of liver fibrosis in cholesterol-induced non-alcoholic fatty liver disease (NAFLD) in rabbits. Methods Forty adult male white rabbits were randomly divided into a normal control group, a model group, a treatment group and a simvastatin group, with 10 rabbits in each group. NAFLD was induced with a high-cholesterol diet. After modeling, the rabbits in the treatment group were intragastrically administrated with Gynostemma Pentaphyllum 5 mg/(kg?d), the simvastatin group with simvastatin 5 mg/(kg?d), and the model and normal control groups with the equal volume of distilled water for 9 weeks. The serum levels of total cholesterol (TC) and triglyceride (TG), the serum inflammatory maker C-reactive protein (CRP), the serum markers of liver fibrosis such as hyaluronic acid and laminin (LN), and the TG level in the liver tissue were detected. Results Compared with the normal control group, the serum levels of TC (60.50 ± 9.77 mg/L vs.1.30 ± 0.44 mg/L), TG (1.72 ± 0.61 mmol/L vs. 0.85 ± 0.39 mmol/L), CRP (256.79 ± 30.78 mg/L vs. 8.71 ± 1.41 mg/L), HA (798.8 ± 69.4 ng/ml vs. 121.2 ± 6.8 ng/ml),LN (964.8 ± 62.8 ng/ml vs. 142.4 ± 12.2 ng/ml) in the model group were increased significantly (all P<0.01). Compared with the model group, the serum levels of TC (36.44 ± 6.57 mmol/L vs. 60.50 ± 9.77 mmol/L), TG (1.31 ± 0.39 mmol/L vs. 1.72 ± 0.61 mmol/L), CRP (68.77 ± 10.78 mg/L vs. 256.79 ± 30.78 mg/L), HA (420.8 ± 60.2 ng/ml vs. 798.8 ± 69.4 ng/ml), LN (449.8 ± 56.6 ng/ml vs. 964.8 ± 62.8 ng/ml) and the TG level in the liver tissue (0.52 ± 0.10 mmol/L vs. 0.77 ± 0.08 mmol/L) in the treatment group were decreased significantly (all P<0.01). The TG level in the liver tissue in the treatment group was significantly lower than that in the simvastatin group (0.52 ± 0.10 mmol/L vs. 0.59 ± 0.09 mmol/L;P<0.05). There were no significant difference in the serum levels of TC, TG,CRP,HA and LN between the treatment group and the simvastatin group (all P>0.05). Conclusions Gynostemma Pentaphyllum may regulate lipid metabolism, alleviate inflammation, and decrease serum markers of liver fibrosis, and might protect against liver fibrosis in rabbits with NAFLD.

Accurate, fast and sensitive typing of pathogenic microorganisms is very important for tracing the source of dissemination, defining the route of transmission, understanding the relationship between strains and clinical symptoms, exploring the pathogenic mechanism,guiding clinical therapy, etc.In recent years, matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS) has played an increasingly important role in the microbial identification, drug resistance and virulence study because of its rapidity, accuracy, good repeatability, high throughput etc.The new microbial typing method-MALDI-TOF MS fingerprint typing appears afterwards.This typing method is characterized by high throughput, rapidity, easy standardization and low cost, and it has promising application prospect in the areas such as epidemiological analysis of pathogenic microorganism, hospital infection control and development of biological markers.

Objective: To study mouse Id3(mId3) protein expression in WEHI-231 B lymphoma cells after CD40 ligand(CD40L) treatment. Methods: The mId3 cDNA was amplified from total RNA of WEHI-231 cells by RT-PCR and introduced into the p3FLAG-CMV-10 vector.The 3FLAG-Id3 fusion gene was amplified from p3FLAG-Id3-CMV-10 vector and subcloned into retroviral vector pMX-CITE/IRES-EGFP.Recombinant retrovirus was produced by transient transfection of the Phoenix-ecotropic packaging cell line with pMX-3FLAG-Id3-EGFP vector using FuGene-6 transfection reagent.(WEHI)-231 cells were transduced with recombinant retrovirus.EGFP expression was monitored by flow cytometry.Two cell lines stably expressing 3FLAG-Id3 fusion protein were generated. Results: The proportion of EGFP-positive cells in the population transduced with pMX-3FLAG-EGFP remained constant up to 2 days after transduction,whereas the proportion in the population of pMX-3FLAG-Id3-EGFP-transduced cells decreased with time.After coculturing with NIH3T3 cells or medium alone,the proportion of EGFP-positive cells in pMX-3FLAG-Id3-EGFP-transduced WEHI-231 cells decreased with time,whereas after coculturing with CD40L-NIH3T3 cells,the proportion of EGFP-positive cells in the transduced cells remained constant up to 2 days,suggesting that CD40L could rescue WEHI-231 from Id3 overexpressioninduced growth arrest.After coculturing with CD40L/NIH3T3 or NIH3T3 cells,3FLAG-Id3 fusion protein stably expressing WEHI-231 cells were harvested,lysed,and immunoblotted by using anti-FLAG antibody.The results showed that treatment via CD40 ligation induced significant downregulation of Id3 expression in pMX3FLAG-Id3-EGFP tansduced WEHI 231 cells. Conclusion:CD40 ligation pvevents the Id3 induced cell growth avvest by the dousn-regulation of Id3 expression.