Objective To observe the real-time and short-term anti-hypertension efficacy of ear apex bloodletting plus acupoint thread embedding. Method Eighty-three eligible hypertension patients were randomized into group A of 28 cases, group B of 29 cases and group C of 26 cases. Group A was intervened by bloodletting at ear apex, group B was by acupoint thread embedding, and group C was by bloodletting at ear apex plus acupoint thread embedding. The blood pressure was recorded 5 min before intervention, as well as 5 min, 15 min, 30 min, and 60 min following intervention, and the real-time anti-hypertension efficacy was compared. By analyzing the blood pressure before treatment and after 1-month treatment, the short-term anti-hypertension efficacy was compared. Result In group A and group C, the systolic and diastolic pressures at 15 min, 30 min, and 60 min after treatment were significantly different from that at 5 min before treatment (P<0.05). The total effective rate was 60.7% in group A, 51.7%in group B, and 80.8% in group C. The therapeutic efficacy of group C was significantly different from that of group A and B (P<0.05). The total effective rates for grade 1 and grade 2 hypertension were respectively 84.6%and 40.4%in group A, 76.9%and 31.3% in group B, and 100.0% and 64.3% in group C. In the three groups, the therapeutic efficacy for grade 1 hypertension was markedly different from that for grade 2 hypertension (P<0.05). Conclusion Bloodletting at ear apex can produce a significant anti-hypertension effect, and bloodletting at ear apex plus acupoint thread embedding is an effective method in treating hypertension.

OBJECTIVE:To investigate the internal quality of 31 batches of Compound Glycyrrhiza oral solutions from different manufacturers.METHODS:The contents of Morphine and Guaifenesin in Compound Glycyrrhiza oral solutions were determined by HPLC,and the pH value of the solution was detected as well.RESULTS:Of the total 31 batches of samples,13 was unqualified in Morphine content,1 was unqualified in Guaifenesin content,and 1 was unqualified in pH value.CONCLUSION:The manufacturers should strictly obey the standards recorded in China Pharmacopoeia in respect of quality control to ensure the safety and efficacy of clinical medication.

BACKGROUND:Matrix metal oproteinases and their inhibitors are proteolytic enzymes contaning Zn+, and involved in extracel ular matrix degradation and tissue remodeling of a variety of tissues. OBJECTIVE:To observe the effects of matrix metal oproteinases in the proliferation of MC3T3-E1 cel s induced by lipopolysaccharide. METHODS:MC3T3-E1 cel line was divided into four groups randomly:control group, low-dose lipopolysaccharide group (1μmol/L), moderate-dose lipopolysaccharide group (10μmol/L), and high-dose lipopolysaccharide group (100μmol/L). The proliferation rate in each group was analyzed. Matrix metal oproteinase 2, 3, 9 and matrix metal oproteinase inhibitor 1 and 2 expressions were detected. RESULTS AND CONCLUSION:The proliferation rate was increased greatly after medication of lipopolysaccharide in time-dependent and concentration-dependent manners. Moreover, the expressions of matrix metal oproteinases and their inhibitors were apparently enhanced, and showed significant differences. Results indicate that the enhanced expressions of matrix metal oproteinases participated in the proliferation of MC3T3-E1 cel s induced by lipopolysaccharide.

The adhesion of cells to each other and to the proteins of the extracellular matrix provides a stable environment for cell growth, differentiation and migration. This is a prerequisite for the normal function of the cardiovascular system. Thus the abnormal adhesion function may play a key role in the pathogenesis and development of cardiovascular diseases. To date, there are six main groups of adhesion molecule, and integrins family are the largest and most broadly distributed of the families of cellular adhesion receptors. They have an important role in several aspects of cardiovascular diseases and that its regulated inhibition leads to a reduction in incidence and mortality due to these disorders. This review focuses upon the structure、mechanism and their roles in cardiovascular diseases which maybe facilitate the development of novel therapies.

Chromatography, Gas ; methods ; Humans ; Tetrachloroethylene ; blood

Objective: To observe the effect of andrographolide on the expression of TNF-α and IL-12 in activated macrophages. Methods: The peritoneal macrophages were harvested from mice intraperitoneally injected with thioglycollate. The macrophages were pretreated with andrographolide and then stimulated with lipopolysaccharide. RT-PCR was used to examine the expression of TNF-α, IL-12a, and IL-12b in the macrophages, and ELISA was used to measure the protein levels of TNF-α and IL-12 in the supernatant. Results: Andrographolide inhibited the mRNA levels of TNF-α, IL-12a, and IL-12b, and the protein levels of TNF-α and IL-12 in activated macrophages, and the inhibition increased with the increase of andrographolide concentration. Andrographolide at 12 μg/ml (P<0.05) and 25 μg/ml (P<0.01) significantly inhibited TNF-α mRNA level in macrophages; andrographolide at 1 μg/ml (P<0.05) and 12 μg/ml (P< 0.01) significantly inhibited TNF-α protein level (P<0.01). Andrographolide at 1 μg/ml significantly inhibited IL-12b mRNA level and at 12 μg/ml significantly inhibited IL-12a mRNA level (P<0.01); and andrographolide at 1 μg/ml also significantly inhibited IL-12 protein level (P<0.01). Conclusion: Andrographolide can inhibit both the expression of TNF-α and IL-12 in activated macrophages.

Objective To investigate the change of disease constitution at different stages of a long voyage on the sea in a frigate. Methods The participants included the crew members in a frigate of PLA navy force. The disease types of the crew members were recorded at different stages of a long voyage on the sea in the frigate, including the whole voyage, early stage of voyage, middle stage of voyage and the late stage of voyage. Then, the disease constitution ratios at different stages was calculated, compared and sorted. Results It was found that the disease constituent ratios during the whole course, from high to low, was: respiratory infection,acute gastroenteritis,lumbar muscle degeneration,insomnia,kinetosis,dermatitis,oral ulcer,conjunctivitis,trauma,disease of cardiovascular system,paronychia and urinary calculi. The ranking during the early stage, from high to low, was: kinetosis, respiratory infection,trauma,acute gastroenteritis,lumbar muscle degeneration,disease of cardiovascular system,insomnia and dermatitis; the ranking during the middle stage was respiratory infection,acute gastroenteritis,lumbar muscle degeneration,insomnia,dermatitis,oral ulcer,trauma,conjunctivitis, urinary calculi and paronychia; and that during the late stage was: respiratory infection,lumbar muscle degeneration,insomnia,dermatitis,oral ulcer,acute gastroenteritis,conjunctivitis,kinetosis,disease of cardiovascular system,trauma,urinary calculi and paronychia. Conclusion Both the disease type and the disease constitution of the crew members change during different stages of a long voyage on the sea in a frigate.

Barium ; urine ; Spectrophotometry, Atomic ; methods

The study of subjcts with T2DM (n=120) and NGT (n=106) in using the screening of PGC-1? gene for SNPs by PCR-SSCP and DNA sequencing showed that four SNPs(Thr394Thr、Gly482Ser、Thr528Thr、Thr612Met) in the PGC-1? gene were found in 50 Han Chinese type 2 diabetic patients.A Gly482Ser polymorphism of PGC-1? gene was associated with type 2 diabetes.[

Objective: To investigate whether high mobility group box chromosomal protein 1 (HMGB1) can activate macrophages to secrete IL-18. Methods: Mouse peritoneal macrophages were cultured with HMGB1 for indicated period, then the mRNA and protein expression of IL-18 and IL-18 receptors were examined by real-time PCR, ELISA, and flow cytometric analysis. Toll-like receptor (TLR) 2 and 4 agonists (1 μg/ml Pam3Cys or 100 ng/ml LPS) were used as parallel controls. Results: Two hours after culture with HMGB1(100 ng/ml), IL-18 mRNA expression was greatly increased. The expression of IL-18 protein was also greatly increased 24 h after culture with HMGB1, and the expression of IL-18R was slightly up-regulated. Pam3Cys (1 μg/ml) and LPS(100 ng/ml) could promote the expression of IL-18 at both mRNA and protein levels in the macrophages, and could up-regulate IL-18 receptors. Conclusion: HMGB1 can directly activate macrophages to secrete IL-18 and to express IL-18 receptor, suggesting that IL-18 may mediate the proinflammatory activity of HMGB1.