Objective Funded papers on pituitary adenoma were bibliometrie analyzed to provide fund information on pituitary adenoma for the researchers.Methods Funded papers on pituitary adenoma from 1994 to 2007 were searched by strategy using pituitary adenoma and fund as key words in Chinese Medical Current Contents.Evaluation of papers on quantity of papers,fund type,level and proportion,the first atuhor,journal,distrier and institute by bibliometries method.Results In 1629 papers collected,there are 110 funded papers,which are comprised of 70 papers supported by National funds(49 supported by National Natural Science Foundation),40 funded by provincial funds.In these papers,there are 65 on basic research(8 on neuropeptide),45 on clinical research(9 on surgery) and 11 are dual funds supported.Of these papers,the first author,journal published on,work institute and distiet,in proper order to Zhang Xiang(7 papers),Chinese Journal of Neurosurgical Disease Research(13 papers),the fourth military university(18 papers) and Shanxi(16 papers).Conclusions The analysis summarizes 49 subjects of National Natural Science Foundation,11 subjects supported by dual funds,core authors and core journals of these papers,which are of great help for researchers' research works.

Objective To investigate the efficacy of pantoprazole and omeprazole in the prevention of postoperative stress ulcer.MethodsThe patients were divided into control group (pantoprazole) and observation group (omeprazole) according to the random number table method from December 2015 to December 2016 in 200 cases of abdominal surgery.The incidence and adverse effects of stress ulcer were measured and the cost and effect were calculated.ResultsIn the observation group, the incidence of stress ulcer was 4.0% (4/100) in the observation group compared with 5.0% (5/100) in the control group(χ2=0.11, P=0.73).Compared with the control group, Both groups were treated with liver and kidney function and blood routine examination, no obvious abnormalities, and no significant adverse reactions during treatment.ConclusionAbdominal surgery patients with pantoprazole and omeprazole can effectively prevent postoperative stress ulcers, the cost of no significant difference, no significant adverse reactions.

This article aims to study the present condition and disadvantages of current obstetrics and gynecology teaching and discuss the urgent needs and methods of evidence-based medicine (EBM) introduction to it.

Cholesteatoma ; diagnosis ; pathology ; Humans ; Petrous Bone ; pathology

Objective To study the expression of interleukin-17 (IL-17) in diabetic rat aorta and the effect of intervention with resveratrol,meanwhile,to explore the potential mechanisms of IL-17 induced diabetic vascular diseases and the protective role played by resveratrol in the epigenetic field.Methods The experiment was carried out in 4 groups:normal control group(NC),normal interventional group(NB),diabetic group(DM),and diabetic interventional group(DB),NB and DB groups were intervened with resveratrol.Immunohistochemistry was used to observe the histological localization of IL-17 and to measure the thickness of rat abdominal aorta.Western blotting,real-time PCR,and methylation-specific PCR were used respectively to compare the expression of IL-17 protein and mRNA,as well as DNA methylation in 4 groups.Results IL-17 mainly expressed in arterial intima of diabetic rats,the abdominal aorta in DM group was obviously thicker than that in NC and DB groups(P＜0.05).IL-17 protein and mRNA expressions in DM group were significantly higher than NC group(P＜0.05),and were reduced in NB and DB groups compared with NC and DM groups respectively.While DNA methylation levels of IL-17 in DM group were significantly lower than NC group(P＜0.01),however,the levels in NB and DB groups were elevated accordingly as compared with corresponding groups.Conclusions The increased levels of IL-17 in aorta of diabetic rats suggests that IL-17 is involved in the process of inflammatory responses to diabetic macrovascular diseases,while resveratrol could inhibit the expression,it may play a role in protecting aorta,and the regulation of IL-17 gene promoter DNA methylation levels may be the potential mechanism underlying these two phenomena.

ObjectTo study the effect of polydatin (PD) and asymmetric dimethylarginine (ADMA) on the endothelial function in aorta vascular strips of healthy rabbits and the interaction between PD and ADMA. MethodsDose-response curves of phenylephrine (PE) on the aortic strips with or without ADMA and/or PD. E max and Kd from PE were obtained and compared. ResultsNormal aortic strips could not respond to ADMA. Pretreatment with either PD or ADMA had no effect on the contractive response of aortic strips to PE. But PD could significantly weaken the contractive response of aortic strips pretreated with ADMA caused by PE in a dose-dependent manner, increase Kd and decrease E max of PE. ConclusionEither ADMA or PD alone does not influence the normal aortic strip contractive functions, the PE affinity to ?-receptor, and E max. But PD could noncompetitively antagonize the contractive reaction of aortic strips to PE in the presence of ADMA.

OBJECTIVETo investigate the effects of different concentrations of Gubishu containing serum on the proliferation of rabbit articular chondrocytes cultured in vitro.

METHODSArticular chondrocytes were obtained from the cartilage of 1-month rabbit and cultured in vitro. They were randomly divided into 8 groups,blank and Gubishu groups in different concentrations (5%, 10%,15%, 20%), MTT assay method was adopted to observe the influence of Gubishu containing serum with different concentrations to the proliferation of chondrocytes after incubated 1, 3, 5, 7 and 9 days.

RESULTSThe proliferation of chondrocytes was dependent on the concentration in Gubishu groups. At same time point,there was significant value between every groups, 20% concentration was greatest (P<0.05); There was significant differences between 5%, 10% and 20% concentration of the blank groups at same time point (P<0.05), and was not between 15% and 20% concentration at the 1, 3, 5 and 7 days (P>0.05), 20% concentration of the blank group was greatest. 20% concentrations of Gubishu containing serum was significantly greater than 20% concentrations of blank group at the 1, 3, 5 and 7 days (P<0.05).

CONCLUSION20% concentrations of Gubishu containing serum can significantly increase the proliferation of chondrocytes, and bring the logarithmic growth period forward to the 3 day.

Animals ; Cartilage, Articular ; cytology ; drug effects ; Cell Proliferation ; drug effects ; Cells, Cultured ; Chondrocytes ; drug effects ; physiology ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; pharmacology ; Female ; Male ; Rabbits ; Serum

OBJECTIVE: To establish a hepatocyte immunotoxicity model for screening of liver protective medications. METHODS: Cytotoxicity was induced by coincubating BCG-pretreated rat hepatocytes in vivo and with 10 mg/L LPS in vitro. Biphenyldimethylesterate (DDB), malotilate(MLT), silybin(SB) and glycyrrhizin (GRZ) were coincubated along with LPS to prevent the hepatocyte injury and verify the applicability and reliability of the model. AST, LDH and nitric oxide (NO) were measured in both the serum and supernatant. The liver and spleen index were calculated and the liver histopathologic changes were examined microscopically. RESULTS: Supernatant AST, LDH and NO in the BCG combined LPS group were increased in comparison with the control group (P<0.01). This increase was attenuated by the addition of DDB, MLT, SB and GRZ (P<0.05). The serum AST, NO and liver and spleen index were also increased significantly compared with the control group (P<0.01). Microscopic exam revealed serious histopathologic changes in the BCG combined LPS group. Hepatoxicity with associated liver enzyme elevation but histopathologic changes were attenuated by DDB, MLT, SB and GRZ. CONCLUSION: BCG combined LPS-induced hepatocyte immunotoxicity in an in vitro rat model may be a useful technique to assess the effectiveness of liver protective medications.

Objective To express the receptor binding domain (RBD) protein of the Middle East respiratory syndrome coronavirus (MERS-CoV) and to characterize the antigenicity of the purified recombi-nant protein. Methods The codon-optimized gene encoding the RBD protein of MERS-CoV was synthesized and then cloned into the pET30a ( +) vector to construct the recombinant expression plasmid. The trans-formed E. coli BL21 (DE3) strains carrying expression plasmid were induced by IPTG under different condi-tions. The expressed products were purified by using nickel affinity chromatography and further analyzed by SDS-PAGE and Western blot assay. Indirect ELISA was performed to analyze the antigenicity and specificity of RBD proteins expressed in prokaryotic expression systems in human serological test. Results The recom-binant RBD proteins were mainly expressed as conclusion body in an optimal induction condition of 37℃ and 0. 5 mmol/ L IPTG for 4 h. The high purified recombinant RBD proteins were obtained through denaturation and renaturation with a relative molecular mass of about 29×103 . Results of the Western blot assay showed that the recombinant RBD proteins could have specific reaction with the serum samples collected form mice with MERS-CoV infection. Indirect ELISA revealed that the RBD proteins expressed in the prokaryotic ex-pression system showed better sensitivity and specificity in the detection of antibodies against MERS-CoV in human serum samples. Conclusion This study reported the prokaryotic expression and purification of RBD protein of MERS-CoV for the first time, which might pave the way for further investigation on immunological detection of MERS-CoV and development of vaccines against MERS-CoV infection.

Objective The aim of the study was to investigate the expression of P-glycoprotein (P-gp)and nuclear factor κB (NF-κB) in the cerebral cortex of rat with chronic fluorosis,and to reveal the mechanisms of damaged nervous system resulted from the toxicity of fluoride.Methods Sixty SD rats were randomly divided into three groups.The rats in each group were given drinking water containing different levels of fluoride:control group less than 0.5 mg/L,small amount of fluoride exposure group 10.0 mg/L and large amount of fluoride exposure group 50.0 mg/L.The animals were examined at the sixth month after initiating the experiment.Protein levels of P-gp and NF-κB in brain tissues were detected by immunocytochemistry and Western blotting,and the P-gp protein and mRNA level by quantitative real time PCR method.Results As compared to the control group(28.21 ±6.13),the numbers of positive staining cells by P-gp antibody in the cortex of rat brains were significantly increased in both the small and the large amount of fluoride exposure groups[(48.46 ± 8.00),(53.72 ± 9.15),respectively,all P ＜ 0.05] ; the protein levels in the control group(100.00 ± 3.86)％ detected by Western blotting were significantly increased in the cortex of rat brains treated with fluoride in both the small and the large amount of fluoride exposure groups[(189.47 ± 3.14)％,(191.36 ± 11.09)％,respectively,all P ＜ 0.05].The significantly increased expression of NF-κB at the protein level was observed in the cortex of rat brains of the small and the large amount of fluoride exposure groups[(365.97 ± 6.04)％ and (417.15 ± 10.89)％,respectively] as compared with the control group[(100.00 ± 10.07)％,all P ＜ 0.05].The mRMA level of P-gp in the cortex of rat brains of the small and the large amount of fluoride exposure groups(2396 ± 427,3479 ± 371,respectively) were higher than that of the control group(260 ± 106,all P ＜ 0.05).Conclusion The increased expressions of P-gp and NF-κB in the cortex of rat brains are induced by chronic fluorosis,which might be connected with the mechanism of brain damages.