1.A research progress of psychological problems and influential factors of senile dementia caregivers
Chinese Journal of Practical Nursing 2017;33(5):392-395
With the decline in cognitive ability, loss of ability to live, the demand of care for the Alzheimer's patients gradually increased. Caregivers tended to bear a greater physical and mental burden. In this study, we found that the caregivers of senile dementia were prone to psychological problems such as depression and anxiety, which may be related to the caregiver's age and caregiving time, and the positive intervention can relieve the psychological problems of the caregivers. This article aims to provide a theoretical basis for nursing workers to explore more effective nursing measures, so as to improve the quality of life of dementia patients and caregivers.
2.Study of fatigue and related factors among children with acute lymphoblastic leukemia receiving chemotherapy in hospital
Xiuqing BU ; Liming YOU ; Ke LIU ; Qimeng YE
Chinese Journal of Practical Nursing 2015;31(13):973-976
Objective To describe fatigue among children with acute lymphoblastic leukemia (ALL)receiving chemotherapy in hospital and explore its related factors.Methods A total of 100 hospitalized children with ALL receiving chemotherapy and their main caregivers were surveyed on demographic information,treatment data,Wong-Baker facial scale and the PedsQLTMMFS Multidimensional Fatigue Scale.Results The incidence of fatigue was 99% (99/100).The total score of fatigue scale was (56.71±17.32) on average.Multiple linear regression modeling showed that the factors related with fatigue included the time of dexamethasone use,childen's sleep quality,hemoglobin content,pain score,the time for the present chemotherapy,age and main caregivers' self-rating health status (P< 0.05 or 0.01).Conclusions Fatigue is serious among children with ALL receiving chemotherapy and is related with demographic factors,diseases,treatment condition and health status of the caregivers.
3.The application of Chinese version of PedsQLTM Multidimensional Fatigue Scale in children with leukemia
Xiuqing BU ; Qimeng YE ; Ke LIU ; Liming YOU
Chinese Journal of Practical Nursing 2015;31(5):323-326
Objective To explore the application of Chinese version of PedsQLTM Multidimensional Fatigue Scale(PedsQLTM MFS) in children with leukemia.Methods 141 children with leukemia and their main caretakers (one parent) were surveyed with the Chinese version of PedsQLTMMFS.Results The Cronbach α was 0.836-0.949 and 0.761-0.930 for parent-proxy scale and children's self-report scale respectively.The correlation coefficients between each item and its subscale were higher than those between each item and other subscales.The scores of parent-proxy scale and children's self-report scale were positively correlated with the correlation coefficient as 0.373-0.738,P<0.01.Conclusions The Chinese version of PedsQLTMMFS has good reliability and validity.It is a reliable scale in measuring fatigue in children with leukemia.
4.Practice and reflection of curriculum integration of basic medicine
Lie QIU ; Qimeng LIU ; Bing GUO ; Qiong HUANG ; Jiaying LIU ; Hong YANG
Chinese Journal of Medical Education Research 2006;0(08):-
According to medicine high special educational reform’s mentality,we have extracted the knowledge points closely related to basic medical curriculum and the nursing spe-cialty,integrated the basic medical curriculum as "the basis of medical morphology"and"basic medical functions"and have it implemented in the nursing specialty in the three-year program.By doing so,we have gained some experience and provide practical basis for further integration of basic medical courses.
5.Study on Chemical Constituents from Petroleum Ether Fraction ofLiparis nervosa
Liang LIU ; Qimeng YIN ; Jia LI ; Dan TONG ; Wanjing ZHANG ; Ziqi LIU ; Zhang CHEN ; Yang HE ; Xinyu SHEN
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(9):1917-1920
This article was aimed to study the chemical constituents of the petroleum ether fraction of Liparis nervosa.Chemicalcompoundswereisolatedandpurifiedthroughvariouschromatographytechniques.The accurate structures of chemical compounds were confirmed with spectral data and literatures. The results showed that7chemicalconstituentswereisolatedfromthepetroleumetherfractionofLiparis nervosa,whichwere moscatin (1), batatasin Ⅲ (2), bergapten (3), isoimpinellin (4), xanthotoxin (5), imperatorin (6) and β-sitosterol (7). It was concluded that chemical constituents 3-7 were isolated from this genus for the first time. And chemical constituents 1-7 were isolated from this plant for the first time.
6.Relationship between SG13S32 locus polymorphisms of 5-lipoxygenase-activating gene and ischemic stroke in Xinjiang Uygur
Meng LIU ; Jianhua MA ; Xiaoning ZHANG ; Qimeng SUN
Chinese Journal of Neuromedicine 2014;13(12):1239-1242
Objective To investigate the relationship of the 5-lipoxygenase-activating (ALOX5AP) gene polymorphisms with ischemic stroke in Uygur Xinjiang.Methods One hundred and ninety-seven Uygur patients with ischemic stroke and 200 Uygur healthy controls in Xinjiang were collected in our study from October 2011 to October 2012.The SG13S32 locus polymorphisms of A LOX5A P gene were determined by using PCR-restriction fragment length polymorphism technique.The case-control analysis was used to analyze the genotypes distributions and allele frequencies.Results There were statistical differences in the distributions of AC genotypes of SG13S32 locus in ALOX5AP gene between patient group and control group (P<0.05) and AC genotypes of SG13S32 locus in ALOX5AP gene significantly increased the risk of ischemic stroke (OR=5.27,95%CI:2.75-11.73).The distributions of all genotypes showed no statistical differences between male and female in the patient group (P>0.05).The distributions of all genotypes showed no statistical differences between patients of different TOAST ratio (P>0.05).Conclusion The ALOX5A P gene SG13S32 locus polymorphisms are associated with risk of ischemic stroke in Xinjiang Uygur population; risk of ischemic stroke does not associate with gender and TOAST ratio.
7.Study on Preparation and in vitro Release Property of Diosmin Gel
Mingyu CUI ; Yuanyuan LIU ; Qimeng HU ; Feng GUAN ; Yingli MA
China Pharmacy 2019;30(20):2778-2782
OBJECTIVE: To optimize the formulation of Diosmin gel and to investigate its in vitro release property. METHODS: Diosmin gel were prepared by using Carbomer 940 as matrix. Using accumulative release rate as index, with the amount of Carbomer 940, ethanol, acetone and pH as factors, L9(34) orthogonal test was conducted. The formulation of Diosmin gel was optimized and validated. Using Diosmin ointment as reference, dialysis bag diffusion method was used to investigate in vitro release property of Diosmin gel prepared by optimal formulation. RESULTS: The optimal formulation of Diosmin gel included Carbomer 940 1.5 g, ethanol 15 mL, glycerol 8 g, pH 6. The gel prepared with optimal formulation was sticky brown-yellow semi solid, and had good coating and spreading properties. The average accumulative release rate (2 h) was (12.67±0.12)%. Results of drug release test showed that Diosmin gel released rapidly within 12 h, then gradually slowed down. The accumulative release rates were (71.93±0.42)% (12 h) and (80.47±0.54)% (24 h), drug release of which were in line with Higuchi equation. Diosmin ointment was released slowly. The accumulative release rates were (41.74±0.18)% (12 h) and (62.63±0.59)% (24 h). Drug release of it were in line with first-order equation. CONCLUSIONS: The formulation of Diosmin gel is optimized successfully. Prepared Diosmin gel has good drug release property.
8.Expression, purification, characterization and application of α-amino acid ester acyltransferase from recombinant Escherichia coli.
Pengfei LIU ; Qimeng LU ; Xueqin HU ; Xuewen HOU ; Hongbin ZHANG
Chinese Journal of Biotechnology 2018;34(7):1169-1177
α-Amino acid ester acyltransferase (Aet) catalyzes the L-alanyl-L-glutamine forming reaction from L-alaine methylester hydrochloride and L-glutamine. In this study, the recombinant Escherichia coli saet-QC01 was used to express the α-amino acid acyltransferase, and its expression conditions were optimized. The recombinant protein was separated and purified by Ni-NTA affinity chromatography, and its enzymatic properties and catalytic applications were studied. The induction conditions suitable for enzyme production optimized were as follows: The temperature was 20 ℃, the induction stage (OD₆₀₀=2.0-2.5), IPTG concentration was 0.6 mmol/L, induction time was 12 h. The optimal reaction conditions of α-amino acid acyltransferase were 27 ℃, pH 8.5, it was most stable between pH 7.0 and 8.0 and relatively stable in an acidic environment, and low concentration of Co²⁺ or EDTA could promote the enzyme activity. Under optimal reaction conditions, 600 mmol/L of L-alaine methylester hydrochloride and 480 mmol/L of L-glutamine, the yield of L-alanyl-L-glutamine reached 78.2 g/L and productivity of 1.955 g/L/min, the conversion rate reached 75.0%. α-Amino acid ester acyltransferase has excellent acid-basei resistance, high catalytic efficiency. These characteristics suggest its application prospects in the industrial production.
9.Xenopus GLP-1-based glycopeptides as dual glucagon-like peptide 1 receptor/glucagon receptor agonists with improved in vivo stability for treating diabetes and obesity.
Qiang LI ; Qimeng YANG ; Jing HAN ; Xiaohan LIU ; Junjie FU ; Jian YIN
Chinese Journal of Natural Medicines (English Ed.) 2022;20(11):863-872
Peptide dual agonists toward both glucagon-like peptide 1 receptor (GLP-1R) and glucagon receptor (GCGR) are emerging as novel therapeutics for the treatment of type 2 diabetes mellitus (T2DM) patients with obesity. Our previous work identified a Xenopus GLP-1-based dual GLP-1R/GCGR agonist termed xGLP/GCG-13, which showed decent hypoglycemic and body weight lowering activity. However, the clinical utility of xGLP/GCG-13 is limited due to its short in vivo half-life. Inspired by the fact that O-GlcNAcylation of intracellular proteins leads to increased stability of secreted proteins, we rationally designed a panel of O-GlcNAcylated xGLP/GCG-13 analogs as potential long-acting GLP-1R/ GCGR dual agonists. One of the synthesized glycopeptides 1f was found to be equipotent to xGLP/GCG-13 in cell-based receptor activation assays. As expected, O-GlcNAcylation effectively improved the stability of xGLP/GCG-13 in vivo. Importantly, chronic administration of 1f potently induced body weight loss and hypoglycemic effects, improved glucose tolerance, and normalized lipid metabolism and adiposity in both db/db and diet induced obesity (DIO) mice models. These results supported the hypothesis that glycosylation is a useful strategy for improving the in vivo stability of GLP-1-based peptides and promoted the development of dual GLP-1R/GCGR agonists as antidiabetic/antiobesity drugs.
Mice
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Animals
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Glucagon-Like Peptide 1/metabolism*
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Receptors, Glucagon/therapeutic use*
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Xenopus laevis/metabolism*
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Diabetes Mellitus, Type 2/drug therapy*
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Glycopeptides/therapeutic use*
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Obesity/drug therapy*
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Hypoglycemic Agents/pharmacology*
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Peptides/pharmacology*
10.Discussion on Material Basis of Different Parts of Mulberry with Different Effects Based on Molecular Connectivity Index
Yuefeng WU ; Zhifei ZHU ; Jinling LIU ; Qimeng FAN ; Youzhi LIU ; Jin ZHOU ; Fuyuan HE
Chinese Journal of Experimental Traditional Medical Formulae 2022;28(13):152-160
ObjectiveTo explore the material basis for the difference in the efficacy of different parts of mulberry based on molecular connectivity index (MCI). MethodBy referring to the relevant literature at home and abroad and traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) database, the chemical composition database of mulberry-source medicinal materials was established. Venn analysis was carried out on the components among mulberry-source medicinal materials. The components in the database were divided into 10 categories, and the composition information was analyzed. According to MCI value, all components of mulberry-source medicinal materials were divided into different groups. The angle cosine method was used to calculate the MCI similarity. The average MCI values of the common component group from 0-8 orders and CI of mulberry-source medicinal materials were calculated. ResultThe components with high similarity such as (+)-cycloolivil, 1′-methoxy-2′-hydroxydihydromollugin, kuwanon, morusin and 1-deoxynojirimycin were selected as potential pharmacodynamic components. Mulberry-source medicinal materials could be divided into five component groups. The similarity between component groups and total components was 0.760-0.999, and the similarity between component groups was 0.248-0.999. In Mori Ramulus, Mori Folium, Mori Cortex and Mori Fructus, the average MCI values of their flavonoids from 0-8 orders were 4.57, 4.59, 6.41, 4.24, respectively. The average MCI values of alkaloids from 0-8 orders were 2.65, 4.55, 2.58, 2.78, respectively. The average CI values from 0-8 orders were 5.51, 5.49, 5.44 and 2.88, respectively. ConclusionIt is preliminarily concluded that there are differences in the flavonoids and pathways of hypoglycemic effects between Mori Cortex and the other three mulberry-source medicinal materials. The MCI values of alkaloids from 0-8 orders in Mori Folium and Mori Fructus were higher, but their inhibitory activity of α-glucosidase were lower than those of Mori Ramulus and Mori Cortex. The structural characteristics of the total components of Mori Fructus represented by CI were quite different from the other three mulberry-source medicinal materials.