Purpose: To analyze the treatment results and detect the prognostic factors for primary gastrointestinal non-Hodgkin'slymphoma (PG-NHL) treated by various treatments. Materials and Methods: 54 PG-NHL patients (19 stage Ⅰ,23 stage Ⅱ, 6 stage Ⅲ and 6 stage Ⅳ patients) were treated from 1983 through 1992. After exploratory,45 patients had gastrotomy and 7, laparotomy. 43 patients were treated by postoperative radiotherapy or chemotherapy or both. Results: The 5-year survival rates ofⅠ～Ⅳ stage were: 94.7%?13.5% ,79.1%?12%, 80%?25.3% and 16.7%? 15.2%. The prognostic factors in PG-NHL were clinical pathologic stages, the volume of the primary tumor and single or multiple foci. Conclusion: Adequate initial surgery combined with postoperation adjunctive R in the upper abdomen for early stage Ⅰ and total abdomen for late stage Ⅰ and Ⅱ, which could be combination C+R following the incomplite resection or unresectable lesions , the tumor dose were 40～45Gy/5～6 wks and the CHOP- BLM program 4～ 6 cyles are benefit for PG-NHL.

Objective:To explore the effect of ChangTong oral liquid(CTOL) on cell cycle of cultured fibroblasts from normal peritoneum(NF) and adhesion(AF).Methods:15 SD male rats were randomly divided into 3 groups:normal serum group,2 CTOL groups(medium and high doses).After administration of medication at the different dosages for 7 days,serums were obtained from the abdominal arteries;the prepared tissues were cultured until outgrowth of fibroblasts.In our study,the passage 3-8 cells were used in order to maintain comparability;The fibroblasts in desired condition were plated in 10% FBS,then divided into three groups:rat serum normal group,CTOL groups(medium and high dose).Cells were cultured respectively for 12,24 and 48 h,and analyzed by flow cytometer.Results:Compared with serum normal group of NF,CTOL medium dosage could increase the proportion of NF in the G1 stage at 12,24 and 48h,but only CTOL high dosage had significance(P

The relationship between intestinal microbiota and functional gastrointestinal disorders (FGIDs) has become one of the hottest topics. The latest Rome criteria states that FGIDs have been more than just gastrointestinal disorders, and that abnormal brain-gut interaction may persist throughout the onset of irritable bowel syndrome (IBS). Intestinal dysbiosis is considered to be an important initiating factor leading to disorder of brain-gut interaction, constituting the microbiota-gut-brain axis, which has become a new research hotspot. Especially in the study of IBS, the intestinal dysbiosis has been confirmed to be related to IBS immune abnormalities and clinical symptoms. Using probiotics to regulate intestinal flora of IBS has achieved good efficacy, and other intestinal flora intervention strategies are being explored.

The clinical application of hybrid surgical technique for treatment of cerebrovascular diseases has gone through a course of more than 10 years. From intraoperative angiography in the treatment of intracranial aneurysms and cerebral arteriovenous malformations at the begining to various applications for more complicated cases at present, hybrid operation has become a new world in the treatment of cerebrovascular diseases. At the same time we notice that the clinical application of hybrid surgery is lack of large-scale multicenter clinical control studies, and there are still different opinions on the indication, technical process,quality control,team building and so on. As a new technique and clinical hotspot,hybrid operation for cerebrovascular diseases has a good prospect,and need to be further standardized.

BACKGROUNDOuabain is a mammalian adrenocortical hormone that is involved in the pathogenesis of hypertension by inhibiting Na-K ATPase activity. It also participates in a variety of kinase-mediated signaling pathways associated with Na-K ATPase. Previous studies have shown that ouabain can cause cardiac remodeling independent of elevated blood pressure and that proliferating cell nuclear antigen (PCNA) plays a coordinating role for numerous proteins involved in multiple processes associated with DNA synthesis. Therefore, we hypothesized that ouabain might play a role in the cerebral cortex through signaling pathways independent of hypertension. And PCNA might be involved in this process.

METHODSMale Sprague-Dawley rats were treated with ouabain or with 0.9% nitric sodium as the control group. Systolic blood pressure was recorded weekly. After four weeks of treatment, morphological changes in the cerebral cortex were analyzed using light and transmission electron microscopy. The expression of PCNA in the cerebral cortex was evaluated by immunohistochemistry, real time quantitative PCR, and Western blotting.

RESULTSAfter 4-week treatment, there was no significant difference in systolic blood pressure compared with the control group, but both structural deterioration and up-regulated expression of PCNA in the brain was induced by ouabain treatment.

CONCLUSIONSThese results suggest that ouabain induces alterations in the brain structure, and this effect is independent of blood pressure. PCNA might be involved in the repair process of ouabain-induced brain damage.

Animals ; Blood Pressure ; drug effects ; Hypertension ; drug therapy ; metabolism ; Immunohistochemistry ; Male ; Ouabain ; therapeutic use ; Proliferating Cell Nuclear Antigen ; metabolism ; Rats ; Rats, Sprague-Dawley ; Real-Time Polymerase Chain Reaction ; Sodium-Potassium-Exchanging ATPase ; metabolism

Candida tropicalis uses alkanes and fatty acids to produce long chain dicarboxylic acids. However, the yield can be affected by β-oxidation in peroxisomes. Pxa1p was a membrane protein of Saccharomyces cerevisiae peroxisomes. Pxa1p and Pxa2p form a dimer that is involved in transporting of long chain fatty acids into peroxisomes, but the similar transporting system of Candida tropicalis has not yet been reported. In this study, a ctpxa1 gene deletion strain named C. tropicalis 1798-pxa1 was constructed by homologous single exchange method using PCR fragment. The expression of ctpxa1 gene in C. tropicalis 1798, C. tropicalis 1798-pxa1 was detected by semi-quantitative RT-PCR, and the ratio of gray value was 2.03, implying that the expression of ctpxa1 in C. tropicalis 1798-pxa1 was weakened. After 144 h fermentation, the dodecanedioic acid production of C. tropicalis 1798-pxa1 was increased 94.3% than the former strain, the maximum yield was 10.3 g/L.

OBJECTIVE To explore the relationship between severe cardiotoxicity caused by oxaliplatin combined with capecitabine and genetic polymorphism， thereby providing references for safe clinical medication use. METHODS Clinical pharmacists conducted a correlation analysis on a case of severe cardiotoxicity in a rectal cancer patient at Qilu Hospital of Shandong University following first-time treatment with standard doses of oxaliplatin combined with capecitabine. Case reports of cardiotoxicity caused by oxaliplatin and capecitabine were retrieved from the Chinese and English databases such as CNKI and PubMed.Basic patient information， drug treatment plan， and cardiotoxic manifestations were extracted and summarized. Combined with the patient’s genetic polymorphism test results related to the metabolism and excretion of platinum-based and fluorouracil drugs， potential mechanisms and prevention strategies for cardiotoxicity induced by oxaliplatin and capecitabine were discussed. RESULTS The patient exhibited homozygous mutations in ABCB1 C3435T and G2677T/A， a heterozygous mutation in MTHFR A1298C， and a heterozygous mutation in GSTP1 A105G， indicating impaired metabolism and excretion of oxaliplatin and capecitabine. The pharmacists recommended discontinuing oxaliplatin and reducing capecitabine to 50% of the original dose for subsequent treatment. The physicians adopted this advice， and the patient experienced no further severe adverse reactions with stable disease progression. CONCLUSIONS Oxaliplatin and capecitabine may cause severe cardiotoxicity. Medical institutions with adequate resources should perform genetic polymorphism test related to drug metabolism and excretion in patients prescribed these agents. For patients with multiple gene mutations， close monitoring and appropriate dose reductions are recommended to ensure medication safety and efficacy.

The coverage rate of HPV vaccination in China is still very low,and the main reason may be related to people's confusion about the choice of vaccine types,the best age of vaccination,vaccination procedures and prices and other factors.This paper briefly describes the etiology of cervical cancer,the approval of HPV vaccine,the application status of HPV vaccine in foreign countries,and the current situation and challenges in the use of cervical cancer vaccine in China,and advocates that obstetrics and gynaecology experts/disease control experts actively promote the prevention and control of cervical cancer,so that young girls and their parents understand the knowledge related to cervical cancer and HPV vaccine,and young girls can be vaccinated with HPV vaccine as early as possible,so as to improve the coverage rate of HPV vaccination and eliminate cervical cancer.

With the popularization and development of lung CT scanning and technology of bronchoscopy in pediatrics,central airway obstruction has been diagnosed more and more.Central airway obstruction can affect airway ventilation and secretion discharge,such children are often hospitalized for repeated pneumonia,and sometimes need mechanical ventilation but may be difficult weaning from it. Malnutrition,hypoxic brain injury and backward growth and development are often associated. In the past,central airway obstruction has been mainly treated by surgery. However,with the development of minimally invasive technology of bronchoscopy in children,airway stent implantation has become an effective and alternative treatment recently. This article reviews the types of pediatric central airway obstruction,indications and contraindication of airway stent implantation,types and selection of stent,and the procedures of stent implantation.

Objective: To understand the status quo of quality of work life and turnover intention of nurses, and to explore the correlation between them, so as to provide scientific basis for formulating nursing intervention measures to improve quality of life and reduce turnover intention. Methods: In December 2018, Random sampling method was used to select 400 nurses from a third-class hospital as the subjects of investigation. The quality of life of nurses was assessed by the quality of work life scale. The turnover intention of nurses was assessed by the turnover intention scale. Results: Nurses' quality of work life was average 2.97±0.59 points, 2.78±0.60 points for job security, 2.61±0.66 points for work welfare, 3.11±0.70 points for job change, 3.34±0.71 points for self-growth, 2.97±0.63 points for environmental support, 2.51±0.80 points for promotion system, 3.41±0.68 points for interpersonal interaction and 3.01±0.72 points for decision-making. Nurses' turnover intention was average 2.46 points. The eight dimensions of quality of work life were negatively correlated with turnover intention I (the possibility of resigning from the present job) and II (the motivation to seek other jobs) (P<0.05) , but not with turnover intention III (the possibility of obtaining external work) (P>0.05) . Multivariate regression analysis showed that age, nurse-job seniority, annual income and self-growth, job change, environmental support, job security and job welfare were negatively correlated with turnover intention I and II (P<0.05) , while night shift frequency was positively correlated with turnover intention I and turnover intention II (P<0.05) . Conclusion The quality of nursing staff's work life is low, which is closely related to their turnover intention. Managers should take targeted measures in some aspects such as environ mental support and promotion system to improve the quality of nursing staff's work life and reduce their turnover intention.