Purpose: To analyze the treatment results and detect the prognostic factors for primary gastrointestinal non-Hodgkin'slymphoma (PG-NHL) treated by various treatments. Materials and Methods: 54 PG-NHL patients (19 stage Ⅰ,23 stage Ⅱ, 6 stage Ⅲ and 6 stage Ⅳ patients) were treated from 1983 through 1992. After exploratory,45 patients had gastrotomy and 7, laparotomy. 43 patients were treated by postoperative radiotherapy or chemotherapy or both. Results: The 5-year survival rates ofⅠ～Ⅳ stage were: 94.7%?13.5% ,79.1%?12%, 80%?25.3% and 16.7%? 15.2%. The prognostic factors in PG-NHL were clinical pathologic stages, the volume of the primary tumor and single or multiple foci. Conclusion: Adequate initial surgery combined with postoperation adjunctive R in the upper abdomen for early stage Ⅰ and total abdomen for late stage Ⅰ and Ⅱ, which could be combination C+R following the incomplite resection or unresectable lesions , the tumor dose were 40～45Gy/5～6 wks and the CHOP- BLM program 4～ 6 cyles are benefit for PG-NHL.

Objective:To explore the effect of ChangTong oral liquid(CTOL) on cell cycle of cultured fibroblasts from normal peritoneum(NF) and adhesion(AF).Methods:15 SD male rats were randomly divided into 3 groups:normal serum group,2 CTOL groups(medium and high doses).After administration of medication at the different dosages for 7 days,serums were obtained from the abdominal arteries;the prepared tissues were cultured until outgrowth of fibroblasts.In our study,the passage 3-8 cells were used in order to maintain comparability;The fibroblasts in desired condition were plated in 10% FBS,then divided into three groups:rat serum normal group,CTOL groups(medium and high dose).Cells were cultured respectively for 12,24 and 48 h,and analyzed by flow cytometer.Results:Compared with serum normal group of NF,CTOL medium dosage could increase the proportion of NF in the G1 stage at 12,24 and 48h,but only CTOL high dosage had significance(P

The clinical application of hybrid surgical technique for treatment of cerebrovascular diseases has gone through a course of more than 10 years. From intraoperative angiography in the treatment of intracranial aneurysms and cerebral arteriovenous malformations at the begining to various applications for more complicated cases at present, hybrid operation has become a new world in the treatment of cerebrovascular diseases. At the same time we notice that the clinical application of hybrid surgery is lack of large-scale multicenter clinical control studies, and there are still different opinions on the indication, technical process,quality control,team building and so on. As a new technique and clinical hotspot,hybrid operation for cerebrovascular diseases has a good prospect,and need to be further standardized.

The relationship between intestinal microbiota and functional gastrointestinal disorders (FGIDs) has become one of the hottest topics. The latest Rome criteria states that FGIDs have been more than just gastrointestinal disorders, and that abnormal brain-gut interaction may persist throughout the onset of irritable bowel syndrome (IBS). Intestinal dysbiosis is considered to be an important initiating factor leading to disorder of brain-gut interaction, constituting the microbiota-gut-brain axis, which has become a new research hotspot. Especially in the study of IBS, the intestinal dysbiosis has been confirmed to be related to IBS immune abnormalities and clinical symptoms. Using probiotics to regulate intestinal flora of IBS has achieved good efficacy, and other intestinal flora intervention strategies are being explored.

BACKGROUNDOuabain is a mammalian adrenocortical hormone that is involved in the pathogenesis of hypertension by inhibiting Na-K ATPase activity. It also participates in a variety of kinase-mediated signaling pathways associated with Na-K ATPase. Previous studies have shown that ouabain can cause cardiac remodeling independent of elevated blood pressure and that proliferating cell nuclear antigen (PCNA) plays a coordinating role for numerous proteins involved in multiple processes associated with DNA synthesis. Therefore, we hypothesized that ouabain might play a role in the cerebral cortex through signaling pathways independent of hypertension. And PCNA might be involved in this process.

METHODSMale Sprague-Dawley rats were treated with ouabain or with 0.9% nitric sodium as the control group. Systolic blood pressure was recorded weekly. After four weeks of treatment, morphological changes in the cerebral cortex were analyzed using light and transmission electron microscopy. The expression of PCNA in the cerebral cortex was evaluated by immunohistochemistry, real time quantitative PCR, and Western blotting.

RESULTSAfter 4-week treatment, there was no significant difference in systolic blood pressure compared with the control group, but both structural deterioration and up-regulated expression of PCNA in the brain was induced by ouabain treatment.

CONCLUSIONSThese results suggest that ouabain induces alterations in the brain structure, and this effect is independent of blood pressure. PCNA might be involved in the repair process of ouabain-induced brain damage.

Animals ; Blood Pressure ; drug effects ; Hypertension ; drug therapy ; metabolism ; Immunohistochemistry ; Male ; Ouabain ; therapeutic use ; Proliferating Cell Nuclear Antigen ; metabolism ; Rats ; Rats, Sprague-Dawley ; Real-Time Polymerase Chain Reaction ; Sodium-Potassium-Exchanging ATPase ; metabolism

Candida tropicalis uses alkanes and fatty acids to produce long chain dicarboxylic acids. However, the yield can be affected by β-oxidation in peroxisomes. Pxa1p was a membrane protein of Saccharomyces cerevisiae peroxisomes. Pxa1p and Pxa2p form a dimer that is involved in transporting of long chain fatty acids into peroxisomes, but the similar transporting system of Candida tropicalis has not yet been reported. In this study, a ctpxa1 gene deletion strain named C. tropicalis 1798-pxa1 was constructed by homologous single exchange method using PCR fragment. The expression of ctpxa1 gene in C. tropicalis 1798, C. tropicalis 1798-pxa1 was detected by semi-quantitative RT-PCR, and the ratio of gray value was 2.03, implying that the expression of ctpxa1 in C. tropicalis 1798-pxa1 was weakened. After 144 h fermentation, the dodecanedioic acid production of C. tropicalis 1798-pxa1 was increased 94.3% than the former strain, the maximum yield was 10.3 g/L.

OBJECTIVETo investigate the relationship between hepatocyte apoptosis and the level of inducible nitric oxide synthase (iNOS) in hepatic tissue in the patients with chronic hepatitis B(Ct-IB).

METHODSWe observed 37 cases with CHB and 10 normal controls. Transferase-mediated-UTP-biotin nick-end labling (TUNEL) technique was used to detect apoptosis cells and immunohistochemical staining were also performed to investigate the expression of inducible nitric oxide synthase (iNOS) in biopsy samples. The serum level of ALT, HBV DNA, grading of necroinflammatory activity and staging of fibrosis were also assessed.

RESULTSHepatocytes in all CHB liver tissues were positively stained by TUNEL in various degree. In contrast, control tissues did not show DNA fragmentation. A significant correlation was seen between apoptosis index (AI) and necroinflammatory grading (r = 0.404, P = 0.015) and serum iNOS level (r = 0.465, P = 0.004). It did not correlate with fibrosis stage and serum alanine aminotransferase level.

CONCLUSIONThe oxidative stress in patients with CHB may reflected the apoptosis of hepatocyte. Apoptosis involves in liver injury of CHB, but with no significant correlation to serum level of ALT.

Apoptosis ; physiology ; DNA Fragmentation ; DNA, Viral ; blood ; Hepatitis B virus ; Hepatitis B, Chronic ; pathology ; Hepatocytes ; pathology ; Humans ; In Situ Nick-End Labeling ; Liver ; pathology ; Nitric Oxide Synthase Type II ; blood ; Oxidative Stress

OBJECTIVETo observe the hypotensive effects of Qindan Capsule (QC) on spontaneous hypertensive rats (SHR) and its effect on the contents of endothelin (ET), calcitonin gene-related peptide (CGRP) and angiotensin-II (Ang-II ) in plasma and vascular tissues, and to investigate the possible mechanism of QC in lowering blood pressure.

METHODSForty SHRs were divided into 5 groups: the high dosage QC group [QCHD, 750 mg/(kgxd)], the low dosage QC group [QCLD, 150 mg/(kgxd) ], the Niuhuang Jiangya Pill group [NJP, 200 mg/(kgxd) ], the Captopril group [ 15 mg/(kg d) land the model group, 8 in each group. Meanwhile, a normal control group consisting of 8 Wistar-Kyoto (WKY) rats was set up also. All the rats were administered with medicine through gastrogavage. Systolic blood pressure (SBP), level of ET, CGRP and Ang-II in plasma and Ang-II in tissues of mesenteric artery were detected in all the rats after 12 weeks of treatment.

RESULTSThe level of SBP after treatment in the QCHD group was lower than that in the model group (P<0.01), but with no significant difference as compared with that in the Captopril group and the NJP group (P>0.05). After treatment, the plasma level of ET was lower and CGRP higher than those in the model group (both P<0.05), and also higher than those in the NJP and Captopril group (both P<0.05). As for the content of Ang-II , in mesenteric arterial tissues, it was lower in the QCHD group than that in the model group ( P<0.05), but in plasma, it showed no significant difference between the two groups (P>0.05).

CONCLUSIONQC has a satisfactory hypotensive action on SHR rats, and its mechanism may be associated with the regulation on plasma vasoactive peptide and regional renin-angiotensin system.

Angiotensin II ; blood ; Animals ; Blood Pressure ; drug effects ; Calcitonin Gene-Related Peptide ; blood ; Capsules ; Endothelins ; blood ; Hypertension ; blood ; drug therapy ; physiopathology ; Male ; Medicine, Chinese Traditional ; Mesenteric Arteries ; chemistry ; Rats ; Rats, Inbred SHR ; Rats, Inbred WKY

Objective: To explore the skin condition and histological changes after face lift based on extended subcutaneous and sub-SMAS undermining and multiple accurate plication of SMAS system. Methods: Eighteen patients that underwent face lift were enrolled according to the inclusion and exclusion criterion. Objective, subjective and histological evaluations were performed to study the skin changes. Results: Nine patients completed the study and skin condition improved significantly after face lift from the objective and subjective results. Histological evaluation revealed that epidermal thickness increased along with dermal collagen content. Collagen and elastic fibers were more condensed after surgery than before, suggesting the dermal regeneration after face lift. Conclusions Skin regeneration after face lift can help maintain the postoperative result. Extensive subcutaneous dissection along with removal of lax skin as much as possible are the key points of face lift.

OBJECTIVE: To treat OSAHS with radiofrequency ablation and evaluate the effect. METHOD: Thirty four patients with mild and median OSAHS were treated with functional endoscopic sinus surgery, UPPP and RFA. The follow up time was 1 year. PSG was used for diagnosis and evaluation of pre- and post-operation. RESULT: AHI of 34 patients reduced from (20.74 +/- 12.23)/h to (12.23 +/- 8.31)/h (P < 0.01). Effective rate was 85.29%. CONCLUSION RFA is an effective, safe and simple assistant therapy for OSAHS.
Adult ; Catheter Ablation ; methods ; Female ; Humans ; Male ; Middle Aged ; Sleep Apnea, Obstructive ; surgery ; Tongue ; Young Adult