Objective To evaluate the clinical efficacy in the treatment of unilateral micrognath ia by using EAM medical resin and hydroxyapatite (EH) composite material combined with the CAD/ CAM technique.Methods From July 2011 to October 2015,12 cases of unilateral micrognathia caused by different reasons were treated,based on the representative traits and requirements,refering to the unsymmetric counter part by right of CAD/CAM technique.By the 3-dimensional design and reconstruction,we reformed the EH composite material into purposed shape,and insert it in the operative area.Results All the materials were closely suitable to the mandible surfaces.1 case failed because the wound was torn apart;the other 11 patients recovered more than 6 months and were satisfied with the external appearance.Conclusions The EH composite materials combined with CAD/ CAM techniques could be a potential characterized remedy for the unilateral micrognathia.

Objective:To evaluate the effectiveness of digital orthognathic surgery in the surgical treatment of patients with hemifacial microsomia (HFM) and patients’ satisfaction.Methods:The clinical data of HFM patients admitted to the Department of Orthognathic & Cleft Lip and Palate Plastic Surgery, Hospital of Stomatology, Wuhan University, from January 2017 to May 2020 were retrospectively analyzed. The digital orthognathic surgery was used to design surgical protocols before surgery. The intermediate wafer and terminal wafer by three-dimensional printing were applied to determine the position of maxillary and mandible bone blocks. The distance change between landmarks in maxillary and chin and the reference planes was measured. The skull CT and face photographs were taken 5 days after surgery to compare the distance between the actual position and the designed position. Data were expressed as the Mean±SD and analyzed by the pairing t-test with P<0.05 considered statistically. The patients’ satisfaction was investigated by interval scale method on day 7 and 6 months after operation. Results:There were 9 HFM patients in this study, included 5 men and 4 women, and the average age was 25.8±3.8 years old. 6 patients were affected on the left side and 3 patients was affected on the right side. All the operations were processed successfully. The wafers were in good position that the maxillary and mandible blocks were moved precisely according to the digital design. There were no accidental fractures of the jaw during the operation. The post-operative photographs and CT showed the stomatognathic system recovered well without serious postoperative complications. The errors between postoperative situation and preoperative designed situation in maxillary and chin were no statistically difference ( P>0.05). The maximum movement error of the maxillary bone block was the mark point of the first molar on the left, with an average error of (0.92 ± 0.34) mm. The patients’ satisfaction scores were averaged 83.2±2.7 points on day 7 after surgery, while the score dropped to 73.8±2.5 points after 6 months. Conclusions:The digital orthognathic surgery technology can satisfy the accuracy requirement of the surgical plan design for HFM patients in correcting the deflect of dental middle line, occlusal plane and chin point. High postoperative satisfaction can be achieved, which may decrease slightly 6 months after operation.

Objective:To evaluate the effectiveness of digital orthognathic surgery in the surgical treatment of patients with hemifacial microsomia (HFM) and patients’ satisfaction.Methods:The clinical data of HFM patients admitted to the Department of Orthognathic & Cleft Lip and Palate Plastic Surgery, Hospital of Stomatology, Wuhan University, from January 2017 to May 2020 were retrospectively analyzed. The digital orthognathic surgery was used to design surgical protocols before surgery. The intermediate wafer and terminal wafer by three-dimensional printing were applied to determine the position of maxillary and mandible bone blocks. The distance change between landmarks in maxillary and chin and the reference planes was measured. The skull CT and face photographs were taken 5 days after surgery to compare the distance between the actual position and the designed position. Data were expressed as the Mean±SD and analyzed by the pairing t-test with P<0.05 considered statistically. The patients’ satisfaction was investigated by interval scale method on day 7 and 6 months after operation. Results:There were 9 HFM patients in this study, included 5 men and 4 women, and the average age was 25.8±3.8 years old. 6 patients were affected on the left side and 3 patients was affected on the right side. All the operations were processed successfully. The wafers were in good position that the maxillary and mandible blocks were moved precisely according to the digital design. There were no accidental fractures of the jaw during the operation. The post-operative photographs and CT showed the stomatognathic system recovered well without serious postoperative complications. The errors between postoperative situation and preoperative designed situation in maxillary and chin were no statistically difference ( P>0.05). The maximum movement error of the maxillary bone block was the mark point of the first molar on the left, with an average error of (0.92 ± 0.34) mm. The patients’ satisfaction scores were averaged 83.2±2.7 points on day 7 after surgery, while the score dropped to 73.8±2.5 points after 6 months. Conclusions:The digital orthognathic surgery technology can satisfy the accuracy requirement of the surgical plan design for HFM patients in correcting the deflect of dental middle line, occlusal plane and chin point. High postoperative satisfaction can be achieved, which may decrease slightly 6 months after operation.

Objective:To investigate the alterations in soft tissue morphology and thickness in the mid-face region of patients with cleft lip and palate (UCLP) secondary to maxillofacial deformity following Le Fort I osteotomy.Methods:A total of 22 patients (16 males and 6 females aged from 17 to 28 years with an average of 20 years) diagnosed with cleft lip and palate secondary to maxillofacial deformity were collected from the Wuhan University Hospital of Stomatology from July 2012 to August 2020. All patients underwent Le Fort I osteotomy. CBCT scans were obtained at T0 (3 days before surgery), T1 (7 days after surgery), and T2 (1 year after surgery). The Dolphin11.95 software and 3D Slicer software were utilized to measure and analyze the soft tissue near the mid-face osteotomy line. Differences in soft tissue thickness before and after surgery were compared.Results:Before and after the operation, the soft tissue thickness at P3, P5, P6, and P8 on the affected side was thicker than that on the healthy side, and the difference was statistically significant, with a P-value of <0.05. At P5, P6, P7, P8, and P9 below the osteotomy line at T2-T0, the degree of postoperative thinning on the affected side was more apparent than that on the healthy side, and there was statistical significance at P6 ( P<0.05). The postoperative soft tissue asymmetry in the Ck region was improved compared with the preoperative one. The preoperative average protruding of the affected side was 0.63 compared with the healthy side, and the postoperative value was 0.17. The preoperative and postoperative Mann-Whitney U tests showed significantly statistical difference. Conclusions:After Le Fort I osteotomy, the facial asymmetry of patients with unilateral cleft lip and palate secondary to maxillofacial deformity is improved. However, there is still a difference in the soft tissue thickness between the healthy side and the affected side, and the change in soft tissue thickness on the affected side is more significant than that on the healthy side.

Osteoclasts are multinucleated cells deriving from the monocyte/macrophage haematopoietic lineage.They contain large amount of tartrate resistant acid phosphatase and cathepsin K and play an important role in resorption of mineralized tissues such as bone and dentine.The resorption capabilities by osteoclasts are thought to be associated with several oral diseases such as periodontitis, periapical periodontitis, peri-implantitis and osteoporosis.Osteoclast size is one of the key evaluating parameters of osteoclast resorption activities.Findings of osteoclast size regulation research may provide a novel breakthrough for the treatment of bone resorption disorder diseases.This article summarized and reviewed the previous relevant experiments and studies of osteoclast size regulation and its mechanism.

Osteoblasts are cells that are in charge of bone formation and play an important role in bone remodeling. Cytoskeleton is widely found in eukaryotic cells, and not only plays an important role in maintaining the order of cell morphology and internal structure, but also may be involved in mechanotransduction, the regulation of cell differentiation, proliferation, apoptosis, migration and the expression of related genes. The studies on the function of the cytoskeleton in osteoblasts may provide new ideas for dental fields in such aspects as guided bone regeneration, orthodontic tooth movement, distraction osteogenesis and postoperative bone healing. This article reviewed the research progresses about cytoskeleton in osteoblasts.

Objective: To study the effect of Jak2-STAT3 pathway on cell proliferation, migration, mineralization and bone defect healing via simulating Jak2-STAT3 pathway inhibitor AG490 to bone marrow mesenchymal stem cells (BMSC) and bone defect mice models. Methods: The effect of AG490 on BMSC proliferation was measured by MTT (methyl thiazolyl tetrazolium) assay. Regulation of AG490 on BMSC migration was tested by scratch assay and transwell assay. The BMSC migration related gene, matrix metalloproteinase (MMP)-7, MMP-9 and CXC subfamily receptor 4 (CXCR4), regulated by AG490 was studied by real-time PCR. Western blotting was adopted to analyze the regulation of Jak2-STAT3 phosphorylation through the simulation of AG490. The alizarin red staining and alkaline phosphatase (ALP) activity assay were performed to measure the effect of AG490 on BMSC mineralization and osteogenic differentiation. Mice femur bone defect models were built to analyzed the effect of AG490 on bone remodeling. Results: AG490 significantly suppressed the migration rate of BMSC at 1 d and 2 d in the experiment group [(12.42±7.50) %, (41.8±2.6)%] compared with the control group [(55.5±9.9)%, (86.9±8.7)%] in scratch assay (P=0.000, P=0.000), the number of migrated BMSC in the experiment group (22.8± 5.9) was significantly suppressed compared with the control group (58.3±6.6) in Transwell assay (P=0.000). The expression of MMP-7, MMP-9 and CXCR4 were significantly downregulated in experiment group [(0.5± 0.1), (0.1±0.1) and (0.35±0.07)] compared with the control group [(1.1±0.1), (1.06±0.33), (1.08±0.13)] (P= 0.0003, P=0.000 and P=0.000). Also, the phosphorylation of Jak2-STAT3 was downregulated by AG490 in western blotting. After BMSCs were osteogenic induced for 14 days, the formation of mineralized nodule and the ALP activity of BMSC is significantly suppressed in experiment group (8.0±2.1) compared with the control group (35.7 ± 1.8) (P=0.0005). AG490 suppressed the bone defects healing,the expression level of phosphorylated Jak2 and phosphorylated STAT3, and the number of alkaline phosphatase positive cell at defect area in vivo is lower in experiment group than the control group. AG490 suppressed the relatively bone density at the defect area significantly (P=0.0004) at 5^th week after the surgery. Conclusions AG490 could suppress proliferation, migration and mineralization to of BMSCs regulate bone defect healing via inhibiting Jak2-STAT3 pathway.