The urokinase type plasminogen activator (u-PA) antigen level in tumorand adjacent tissues was evaluated in 35 cases of gastrointestinal carcinomas and lungcancer with the u-PA ELISA sandwich method developed in our laboratory. The concentrations of u-PA antigen in 5. 0ml of extracts, each of them made from0. 5 gram tumor tissue, were 118. 4?121. 1, 138. 8?115. 8, 103. 3?56. 7, 85. 8?54. 5 and197. 4?86. 3 ?g/L for the cases of gastric (n=10), esophagus (n=8), colon (n=6),rectum (n=6) carcinoma and lung cancer (n=5), respectively. It showed that the u-PAantigen levels in all the 5 kinds of tumor tissue were significantly higher than that oftheir adjacent tissues (P

Objective To evaluate the possible association between depression and cellular immunologic status in patients with condyloma acuminatum (CA). Method Depression was assessed with the Self-rating Depression Scale (SDS) and the activity of natural killer (NK) cells, the interleukin-2 (IL-2) produced by peripheral blood mononuclear cells (PBMC) were measured in 60 patients with CA. Results The SDS scores in patients with CA were significantly higher than those in the controls (P 3 months) were significantly higher than those in patients with short duration (≤3 months) (P

Objective To study the relationship between exon gene polymorphism of pulmonary surfactant pro-tein B(SP -B)and the susceptibility and severity,prognosis of respiratory distress syndrome (RDS).Methods To detect the gene sequence of SP -B exon by adopting the gene sequencing technology,and samples were 80 prematures of very low birth weight in Southern Han Chinese,who were divided into 2 groups,the RDS and the non RDS,and the difference of genotype in SP -B exon in 2 groups was compared.Results There was no significant difference between 2 pretem groups in the aspects of the gestational age,sex,birth weight and delivery mode etc(all P >0.05).Fifty -nine prematures of very low birth weight had exons heterogenesis,and there were 2 types of mutations,V1 :Exon2:c.[5A >C]+[5A >C]or c.[5A >C]+[=];V2:Exon5:c.[428C >T]+[428C >T]or c.[428C >T]+[=].There were 20 cases of type V1 ,1 8 cases of type V2,3 cases of type V1 +V2 in 45 cases of RDS,and there were 1 2 cases of type V1 ,9 cases of type V2,no case of type V1 +V2 in the non RDS group.Comparing the incidence of V1 and V2 in 2 groups,there were all significant differences(χ2 =3.73,5.02;all P <0.05),and the OR values of 2 polymorphisms to RDS were 3.33 and 4.00,but there was no significant difference in mutation cases and non mutation cases in RDS group when comparing severity and prognostic(χ2 =0.07,P >0.05).Conclusion Gene polymorphism of SP -B exon are risk factors for premature of very low birth weight in southern Han Chinese in RDS.

By means of cell hybridization in situ with digoxigenin labeled probe, we detected the expression of C-myc protooncogene in peripheral blood mononuclear cells from 31 SLE patients and 10 healthy persons. The results showed that the level of C-myc mRNA in SLE patients was significantly higher than that in normal controls, and the increased level correlated with disease activity. There was also a positive correlation between C-myc mRNA and serum levels of ANA, anti-dsDNA, IgG, C3. Our study indicates that C-myc gene is important for the pathogenesis of SLE.

ObjectiveTo explore the control factor of pulmonary microvascular endothelial cells in pulmonary hemorrhage with the RhoA-Rock pathway inhibitors.MethodsHuman pulmonary rnicrovascular endothelial cells were conventionally cultured,and were divided into four groups:control group,inhibitor group,hypoxia group and hypoxia group with inhibitor.As different fluorescein lsothiocyanate-phalloidin and filamentous actin (F-actin) in cytoplasm combined,it issued red fluorescence.We observed the dynamic changes of F-actin by laser scanning confocal microscope in hypoxia human pulmonary microvascular endothelial cells and recorded the value of fluorescence.ResultsThe mean fluorescence intensity of F-actin of hypoxia group in 1 h,12h and 24 h was (64.3 ±5.5)％,(60.3±4.2)％,and (47.8 ±4.6)％ as compared with the control group;the ratio of hypoxia group with inhibitor was (66.2 ±3.2)％,(67.1 ±6.2)％,and (72.5 ± 6.1 ) ％ as compared with the control group.The mean fluorescence intensity of F-actin decreased obviously after 1 h hypoxia treated to cells,decreasing to (64.3 ± 5.5 ) ％ of the control group (P ＜0.05 ) ;to 24 h,decreasing to (47.8 ±4.6) ％ of the control group(P ＜0.05).The mean fluorescence intensity of F-actin decreased to (66.2 ± 3.2) ％ of the control group after 1 h hypoxia treated in inhibitor group,which was more than 1 h in the hypoxic group.F-actin decreased obviously to (72.5 ± 6.1 ) ％ of the control group after 24 h hypoxia treated in inhibitor group.There was significant difference comparing with the hypoxia group after 24 h hypoxia(P ＜0.05).The mean fluorescence intensity of F-actin of inhibitor group without anoxic was invariant comparing with the control group(P ＞0.05).Cortex-like structure disappeared and the stress fibers arranged disorderly after hypoxia.Actin depolymedzated and broke gradually with the extension of hypoxia time.If to be hypoxic after pretreated with RhoA-Rock pathway inhibitor,cortex-like structure by the composition of pednuclear F-actin reappeared,distribution and arrangement of stress fibers in the cytoplasm tended to rule.ConclusionThe RhoA-Rock pathway mediates the damage on F-actin of pulmonary microvascular endothelial cells after hypoxia.Interfering with the RhoA-Rock pathway inhibitors can provide a new direction for the treatment of neonatal pulmonary hemorrhage.

[Objective]To explore the results of the treatment of the ischemic necrosis of femoral head using titanium alloy cage in animal models.[Method]The ischemic necrosis of femur head was made with the liquid nitrogen in 15 hybrid adult dogs.The titanium alloy cage of threaded central empty cylinder with multi-hole periphery was driven into the full depth of the center decompression canal to the subchondral bone.The dogs were divided into 3 groups,5 dogs in each groups were sacrificed respectively at 3,6,12 weeks after operation.Radiograph and histologic examination were used to evaluate the results.[Result]No collapse of femoral head was observed postoperation.The position of the cages was located wall in X-ray films.Under microscope,the cancellous bone of necrotic femoral head rebuilted up gradually.The cancellous bone grew into cage.After 12-week creeping substitution,the cancellous bone filled up the hollow and the peripheral holes of the cages.[Conclusion]The titanium alloy cage can provide structural support to the subchondral bone and prevent collapse.The cancellous bone grows into the cages through the peripheral holes.The necrotic femoral heads are restoration.The titanium alloy cage could be recommended for the treatment of the ischemic necrosis of femoral head.

In this article,we analyzed E-leaning's characteristics and trend of development,and then we put forward an opinion that in such situation students should have a capacity of making use of network to have autonomous study without the limit of time and space,and at the same time teachers also should use the information technology to train students' capacity of evaluating and processing information,in order to be able to conduct the self-management learning through the network.

Objective To explore the effect of Vitamin K1(Vit K1), fresh frozen plasma (plasma) andcryoprecipitate on prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen original(Fbg), thrombin time (TT) of newborns with different gestational ages. Methods The serum of 1,134 newbornsfrom The Third Affiliated Hospital of Guangzhou Medical University was collected from February 2009 to September2012. All newborns had been divided into four groups (according to the gestational age of 28-31+6 weeks, 32-33+6weeks, 34-36+6 weeks and gestational age≥37 weeks).The effect of various interventions (Vit K1, Vit K1+plasmaand Vit K1+cryoprecipitate) on PT, APTT, Fbg, and TT had been recorded. Results (1)The PT and APTT ofeach group with the interventions of Vit k1 were significantly improved (P < 0.05). (2)The PT, APTT, Fbg and TTof each group with the interventions of Vit k1 combined with plasma were significantly improved (P < 0.05). (3)ThePT, APTT and Fbg of each group with the interventions of Vit k1 combined with cryoprecipitate were significantlyimproved (P < 0.05). (4)With Vit k1 combined with plasma, PT and APTT were mostly improved and Fbg wasimproved mostly with Vit k1 combined with cryoprecipitate. Conclusion Vitamin K1, fresh frozen plasma andcryoprecipitate can effectively improvedin the coagulation index of newborns with different gestational ages.

Objective:To find a feasible method to stimulate tumor-draining lymph node(TDLN) cells in clinic.Methods:CTL activity of TDLN cells induced by different stimulus (IL-2 group, IL-2+autologous tumor antigen group, IL-2+GM-CSF+IL-4+autologous tumor antigen group) was measured by the method of maximal LDH enzyme release. The mechanisms were explored by observation in morphology and detection of the CD83 positive rate of TDLN cells.Results:The level of growth of TDLN cells induced by (IL-2+GM-CSF+IL-4+autologous tumor antigen) was significantly higher than TDLN cells induced by IL-2 and (IL-2+autologous tumor antigen)(P

BACKGROUND: Due to the trouble of body image and self-esteem, more and more women hope to reduce their body mass.OBJECTIVE: To assay the characters and correlation between body image and self-esteem in women who reduce weight positively.DESIGN: Transect investigation.SETTING: Department of Dermatology of Xiangya Hospital and Xiangya Second Hospital of Central South University. PARTICIPANTS: Totally 100 patients for weight loss was collected in Department of Acupuncture and Moxibustion of Xiangya Hospital Affiliated to Central South University from November 2002 to September 2003 and they were included in experimental group. Severe physical disorders or other chronic physical disorders were excluded, such as cardiac vascular disease and diabetes and various metal disturbances were excluded too. In addition,the patients did not taken psychoactive drugs recently. Those were in over-body-mass group if body mass index(BMI) ≥ 24 and those were in normal body mass group if BMI＜24. In the control, there were 100 healthy women participating in the study in volunteer at the same time. The exclusive standards for them were same as the experimental group and they did not taken psychoactive drugs recently and had done nothing for weight loss in 5 years.METHODS: After instructed, the receptors filled up the following questionnaire: a self-rating scale of body image(SRSBI) and the self-esteem scale (SES). SRSBI is the subjective assessment on the beauty and strength of the body individually. SES is the assessment on holistic perception of self-value and self-acceptance.MAIN OUTCOME MEASURES: Results of SRSBI and SES in weight loss women.RESULTS: According to intention, general results of SRSBI: The score in experimental group(15.14±9.99) was higher than that in the control (7.89±6.18) (t= 6.15, P＜0.001) . The score was(15.65±10.36) in normal body mass group and was(13.81 ±10.03) in over-body-mass group. By the comparison between both, no difference presented(t=0.91, P ＞0.05) . Results of SES: the score in experimental group (27.01±4.19)was lower than that in the control(28.23±3.65) (t= 2.16, P＜0.05).The score was(27.31±4.50) in normal body mass group and was (26.86±3.82) in over-body-mass group. By the comparison between both,no difference presented (t=0.54, P＞0.05) . It was indicated in linear correlation analysis on SRSBI and SES in experimental group that the correlation coefficient between SRSBI and SES was-0. 088(P＞0.05).CONCLUSION: The people who seek for weight loss actively present inferior subjective perception of body image and low level of self-esteem, and there is no correlation between both of them and neither of them is relevant t.o obesity.