1.The comprehensive observation of the curative effect of the female chronic pelvic pain
Lei XIE ; Xiaoyong YU ; Qihui YE
Chinese Journal of Primary Medicine and Pharmacy 2006;0(07):-
Objective To search the effective treatment for the female chronic pelvic pain.Methods The drugs of ?-receptor inhibitor,antianxietic,psychotherapy and proper movement were taken.It was six weeks for a course of treatment.Results Of the study group(48 cases),complete recovery were noted in 36 cases,remarkable improvement in 5 cases,remarkable inefficacy in 4 cases,the total curative rate was 91.66%,which was significantly better than the control group(60.42%).Conclusion It is an effective treatment for the female chronic pain.
2.Comparison of ureteroscopy pneumatic lithotripsy and extracorporeal shock wave lithotripsy in the treatment of distal ureteral calculi
Lei XIE ; Xiaoyong YU ; Wenjie LI ; Qihui YE ; Chaofan XIE ; Honghui XIA
Chinese Journal of Primary Medicine and Pharmacy 2006;0(06):-
Objective To evaluate the therapeutic effect and safety of ureteroscopy pneumatic lithotripsy(PL) and extracorporeal shock wave lithotripsy(ESWL) in the treatment of distal ureteral calculi.Methods 368 cases of distal ureteral calculi were divided into the PL treatment group(178 cases) and the ESWL treatment groups(190 cases).The clinical datas were compared between the two groups.Results PL treatment group 97.19% patients became stone free in 4 weeks,and in ESWL treatment group the stone free rate was 73.16%(P
3.The effect of a motor relearning programme combined acupuncture on muscle tension and motor function recovery after cerebral infarction
Xu ZHANG ; Qihui LI ; Yingying LI ; Chuanbang CHEN ; Junyan LU ; Beilei ZHU ; Ruixian ZHENG ; Chun LIAN ; Tianshen YE
Chinese Journal of Physical Medicine and Rehabilitation 2012;34(4):279-282
Objective To observe the effects of a motor relearning programme (MRP) combined with different early acupuncture interventions on muscle tension and motor function recovery after cerebral infarction.MethodsA total of 90 patients with cerebral infarction who met the inclusion criteria were divided into three groups at random:a YANGMING meridian acupuncture and MRP group ( group A),an anti-spasm acupuncture and MRP group ( group B),and an MRP group ( group C ).All of the patients in all three groups were treated with routine medication.The National Institute of Health stroke scale (NIHSS),the composite spasticity scale (CSS),Fugl-Meyer assessment (FMA),the Fugl-Meyer balance scale (FM-B) and the modified Barthel index (MBI) were used to measure performance before treatment and after 4 weeks of treatment.Another comparison was intra-group between before and after treatment. ResultsThere were significant differences in the assessment results in all of the groups after treatment compared with those before treatment.After treatment,group B was superior to group C only in terms of NIHSS scores.There was no significant NIHSS score difference between groups A and C.The FMA,CSS and MBI results revealed significant differences among all three groups,with the scores of group A consistently the highest.The average FMA score in group B was significantly higher than in group C but there was no statistically significant difference in FM-B scores among the three groups. ConclusionMRP therapy combined with early acupuncture intervention can improve motor function and muscular tension after cerebral infarction.Anti-spasm acupuncture can improve motor function and control muscular tension effectively at the same time,making it beneficial for MRP training.
4.Expression of the hemagglutinin and neuramidinase gene of influenza A virus H1N1 in Pichia methanolica.
Ye ZHANG ; Zaijiang YU ; Li XIN ; Yongkun CHEN ; Qihui TANG ; Yubao CHEN ; Qingxuan CHEN ; Yuelong SHU
Chinese Journal of Biotechnology 2010;26(8):1068-1073
On the basis of successful cloning the full length hemagglulinin (HA) and neuramidinase (NA) gene and sequence analysis of influenza virus H1N1, part of the gene was ligated into pMETA. Expression vectors pMETA/HA (52-1 557 bp) and pMETA/NA (121-1 263 bp) were constructed and expressed in pMAD16 induced by methanol. Recombinant protein was purified through Ni2+ affinity chromatography. Western blotting and ELISA were used to determine the antigenic activity of the recombinant protein. SDS-PAGE showed that the recombinant capsid gene could be overexpressed in Pichia methanolica. ELISA and Western blotting showed that the recombinant protein had antigenicity.
Cloning, Molecular
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Genetic Vectors
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genetics
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Hemagglutinins
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biosynthesis
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genetics
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Influenza A Virus, H1N1 Subtype
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genetics
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Neuraminidase
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biosynthesis
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genetics
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Pichia
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genetics
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metabolism
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Recombinant Proteins
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biosynthesis
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genetics
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immunology