Objective To evaluate the effect of limb lengthening on the perineurial barrier and vascular permeability of peripheral nerve. Methods Thirty-two rabbits with limb lengthening of 10％ , 20％ , 30％ and 40％ were used in this study; 8 animals were allocated in each group. Sixteen were employed for the electron microscopic(EM) observation and the others for the investigation of perineurial barrier and vascular permeability of the tibial nerves. Results Even if the elongation reached 40％ of the original length of the tibia, both the perineurium and inner membrane of capillary was still intact, the perineurial barrier and vascular permeability maintained normal; however, EM examination revealed increase of a large number of vacuoles and the increase of the number of mitochondria in the cell of the perineurial membrane. Conclusion It is the gradual limb lengthing of

The pathogenesis of migraine has not been clarified yet.The cnrrent dominate theories on the pathogenesis of migraine included:vasogenic theory,neurogenic theory,biochemical factors,genetic factors,and infection factors.Study progress migraine in recent years was summarized in the paper.

Objectives:To investigate the relationship between coronoid process hyperplasia(CPH)and temporomandibular joint ankylosis(TMJA) and to analyze its pathologic mechanism and clinical meaning.Methods:28 patients with TMJA from 1984 to 2004 were retrospectively studied.The length of coronoid process and the mouth-opening degree were measured on their depicting map of panoramic radiographs.106 subjects with normal TMJ were enrolled as the controls.The relationship between CPH and case history of TMJA was statistically analysed.Results:Coronoid process in TMJA subjects was longer than that in the controls(P

Objective To establish the measurement for evaluating the efficiency of isolating and harvesting marrow mesenchymal stem cells (MSCs) and observe the main biological characteristic of MSCs in vitro during the passage cultivation. Methods ①The marrow nucleated cells in young rabbit marrow were isolated by the gradient centrifugation and cultivated to isolate and harvest MSCs. The efficiency of isolating and harvesting MSCs was measured by the number of the first passage MSCs from every 10~(6) of the marrow nucleated cells. ②The changes of MSCs morphology, growth and proliferation, synthesis of collagen typeⅠand activity of alkaline phosphatase (ALP) were observed during the passage cultivation. Results ① After cultivated at 2.5?10~(5)/cm~(2) marrow nucleated cells for 9-10 d, the first passage MSCs were harvested. The number of the first passage MSCs had a remarkable statistical positive correlation with the number of the marrow nucleated cells from one rabbit(r=0.932, P

OBJECTIVE:To observe clinical efficacy and safety of thymosin in adjunctive treatment of chronic eczema,and to investigate its mechanism from the change of peripheral T lymphocyte subsets. METHODS:160 patients with chronic eczema were randomly divided into observation group and control group,with 80 cases in each group. Both group received conventional therapy as mizolastine,Desonide cream. Observation group additionally received Thymosin enteric-coated tablet 20 mg,po,tid;control group was given placebo the appearance of which was same with Thymosin enteric-coated tablet. Treatment course lasted for 4 weeks. The change of T lymphocyte subsets,clinical efficacy and the occurrence of ADR were compared between 2 groups. Re-currence rate of both groups were followed up for 1 year. RESULTS:The effective rate of observation group(91.25%)was signifi-cantly higher than that of control group(77.50%),with statistical significance(P<0.05). After treatment,CD4+ and CD4+/ CD8+ of 2 groups were significantly increased,while CD8+ was decreased significantly;CD4+ and CD4+/ CD8+ of observation group were significantly higher than those of control group,with statistical significance(P<0.05),but there was no statistical significance in CD8+ between 2 groups(P>0.05). There was no statistical significance in the incidence of ADR between 2 groups(P>0.05). Re-currence rate of observation group(31.51%)was significantly lower than that of control group(64.52%)during follow-up period, with statistical significance (P<0.05). CONCLUSIONS:In summary,thymosin can improve clinical efficacy and reduce recur-rence rate by regulating T lymphocyte subsets with good safety.

Budd-Chiari syndrome (BCS) is a rare disease. Because of lack of clear characteristic symptoms in the early stage, many patients are misdiagnosed or never diagnosed. Early diagnosis and prompt treatment play a key role in improving survival rates and quality of life for BCS patients. With the progress in imaging techniques, the radiological examination is playing more and more important role in the diagnosis and assessment of BCS. Ultrasound, computed tomography, magnetic resonance imaging and digital subtraction angiography are all helpful in evaluating the obstruction of hepatic vein and inferior vena cava. Thus, these imaging tests should be used rationally according to their features. On the other hand, the interventional therapy is the preferred method for the treatment of BCS. This paper intends to get a deeper understanding of BCS for clinicians, which is essential for patients to be diagnosed in time.

Objective To investigate the adaptation of metabolism of arthrodial cartilage for limb lengthening. Methods Thirty New Zealand rabbits were randomized into two groups, limb lengthening group (group A) and delay observation group (group B). Every group was subdivided into three sub-groups according to distraction degree: 10%, 20% and 40%. Upper-tibia metaphysis lengthening was adopted at 1 mm/d rate of distraction and iconography changes of knee joint were observed with X-ray, the morphologic changes of chondrocyte and cartilago articularis on tibial flat were observed with light and electric microscope. The changes of cartilage surface were observed with scanning microscope. Results 1) There was no obvious iconography change of knee at every time phase in each group. 2) Pale of cartilage on tibial flat were just noted in group A40 and B40. 3) The arrangement of chondrocyte was disorder in A40, some degenerating and generating chondrocytes were found in the same group. The number of chondrocytes increased, and the volume was expanded in group B20 or B40, the degenerating chondrocytes were not found in the same group. 4) Low-grade expansion of endoplasmic reticulum and swelling of chondrosome were found in part of chondrocyte in group B40 contrasting with the other groups. The numbers of endoplasmic reticulum and matrix alveolus increased in chondrocyte. The same changes were observed in group A. 5)The cartilage surface lost its smoothness during distraction, and this kind of changes were more severe when distraction increased. Conclusion The articular cartilage showed certain adaption to stimulation of distraction during low degree distractions. To greater distraction degrees some abnormality in metabolism of arthrodial cartilage may occure.

Objective To study the feasibility of polylactic acid(PLA) as carrier of bone morphogenetic protein(BMP), and observe its results of inducing osteogenesis. Methods A 12 mm bone defect experimental model in the left ulna of rabbits was made, the animals were divided into three groups at random, bovine BMP 10 mg with PLA carrier was implanted into the defect area in the experimental group, bovine BMP 10 mg with bovine cancellous bone matrix carrier was implanted in the control group, and nothing was implanted in the blank group. The osteogenesis of the bone in the defect area was observed by regular roentgenography, the histological changes of the bone defect tissue in 4th, 8th, 12th week after operation were studied and the new bone formation was measured by image analysis. Results The results revealed that the bone defect in the experimental group had excellent repair than that seen in the control and blank group, both bone union and the period of bone mature were earlier than that seen in the control and blank groups. There was fibration in the blank group at 12th week. Conclusion The result indicated that PLA is a material able to be used as a carrier of BMP in inducing osteogenesis, it has a better effect of inducing osteogenesis than xenogenous cancellous bone matrix. Compared with other carriers, PLA has the following advantages: 1)It releases BMP gradually during degradation, so the BMP concentration in bone defect can be maintained effectively. 2)It is a material with some rigidity, so is able to exert flexible fixation of the fractures. 3)It can be shaped to adjust itself for the individual fractures. 4)It has a good histocompatibility and an adjustable property. However, the relationship between the molecule weight, aperture of PLA and the best inducing osteogenesis need a further study.

In order to investingate the stability of the half-ring sulcated external fixator,it was used to fix the bone after artificial fracture of the tibia shaft and tibia lengthening with proximal metaphyseal osteotomy were performed on 20 and 22 goats respectively.After operation,the animals were allowed to roam about freely,walking or jumping.In the fracture group,radigraphy of the tibia shaft,strength determination of the limb,and histological study of the fractured site were performed at the end of the 6th week.And in the lengthening group,x-ray examination and histological study were done after the 10th week.All the findings indicate that the external fixator designed in our department is well qualified to meet the requirement for the fixation of fractrued bones and the lengthened bones as well.

Transverse fracture was inflicted to the tibia of goats and specially designed external fixator was used for fixation to observe the relationship of different intensity of compres-sive force with fracture-healing.It was found in vitro test that the external fixator possessed less effect of stress protection.X-ray examination revealed that the stronger the compressive force was applied,the later the external callus would appear and the less amount of external callus would form.In the 6th week after fracture,the fracture line became oblierated in cases with mild compression while a transparent line in the external callus could be seen on x-ray films in those cases with heavy compression.Though there was no significant difference of the parameters of torsionmetry among different groups,the strength of the healed fracture with mild compression was nearer to the strength of a normal bone than that with heavy compression.Histo-logical examination revealed that all the bone unions belonged to secondary bone healing and bone callus became less matured along with the increase of compressive force.