Objective In order to sum up the cervical manifestation of the Hodgkin's disease.Methods A retrospective review was made on 46 cases of the Hodgkin's disease involving the neck between 1999 and 2002.Specially the clinical presentations,staging for the Hodgkin's disease involving the neck was sought.Results The Hodgkin's disease occurs in any ages,especially the younger and the old.The diameter of lymph node of neck is usually under 5 cm,the biggest reaches 16 cm.Lymph node of neck in the side region of neck is most common,and the anterior region of neck takes the second place.The nod sclerosis type and the mix cell type are most common in the Hodgkin's disease.Conclusion We must learn the law of the Hodgkin's disease involving the neck,if only we do this,we will detect the disease early and treat early.

Simulating physiological neuronal and hormonal conditions during digestive and interdigestive periods, the study identified the changes of the motility of biliary system including bile duct and sphincter of Oddi (SO) before and after cholecystojejunostomy. Thirty-five rabbits were divided into five groups randomly. The experimental groups received the venous injection of CCK 10 ng/kg, erythromycin 10 mg/kg, atropine 3 micrograms/kg and L-NAME 10 mg/kg respectively. Each rabbit underwent manometry through introducing a three-lumen catheter via the papilla retrogradely, using the low-compliance papillary infusion system. Then the gallbladder and the upper segment of the jejunum was anastomosed and the manometric procedures repeated after one week. SO basal pressure was increased, contraction amplitude decreased, contraction time shortened after cholecystojejunostomy. L-NAME, CCK and erythromycin could all excite SO. L-NAME could increase basal pressure and contraction amplitude, CCK increase basal pressure contraction amplitude and frequency, and erythromycin increase contraction amplitude, respectively. But comparing with that before cholecystojejunostomy, the increasing extent was decreased. The tensional and spontaneous contractions of the SO were under the control of the neural and hormonal mechanism. The anastomosis of gallbladder and jejunum and the drainage of bile made the tensional contraction stronger, but the spontaneous contraction weakened after the operation due to the decreases of the sensitivity of SO to hormonal factors. The clinical symptoms may not be relieved when the patients with SO dysfunction accepted cholecystojejunostomy.
Cholecystectomy/*adverse effects ; Common Bile Duct/*physiopathology ; Gallbladder Emptying/*physiology ; Jejunostomy/*adverse effects ; Manometry/methods ; Muscle Contraction ; Postoperative Period ; Random Allocation ; Sphincter of Oddi/*physiopathology

In order to study the effects of biliary high pressure on splanchnic major nerve and the its significance during sever type acute cholangitis(ACST), using a self-made catheter with a blind cyst at the biliary tract, an observation was completed on biliary compression of 150mmHg (20kPa) in big ear white rabbits, nervous impulse frequercy of splanchnie major nerve and blood pressure was measured at the same time. The results showed that a significant decrease in blood pressure(P0.05), the authors believe that activity of splanchnic major nerve is related to the decrease of blood pressure in earlier stage of ACST.

In order to study the effect of acute biliary hypertension on cardiovascu-lar activity, an experiment has been done on Japanese big ear white rabbits, using a self-made catheter with an inflatable rubber bag fixed to its anterior end. It was insertedinto the extrahepatic biliary tract via the duodenum, biliary high pressure of 8-20 kPawas created and maintained for 2 h by inflating the bag with water. A significant decreaseof cardiac output (CO) and fall of the arterial blood pressure were found during theexperiment (P0.05). These results indicated that the increase ofthe biliary pressure without infection could also make CO decrease, so it was a direct rea-son for falling of blood pressure, it was also an important factor causing the decline ofblood pressure in the ealier stage of severe acute cholangitis (ACST).

Objective To study the influence of siRNA targeting survivin on chemotherapy sensitivity of HCC cells.Methods siRNA eukaryotic expression vector was generated.After the vector stablely transfected into HepG2 cells,the effects of chemotherapy drugs on HCC cells were observed.Results The recombinant plasmid Psilence(+)-survivin was successfully constructed.Survivin mRNA expression inhibition ratio reached 73% detected by RT-PCR.MTT methods detected that siRNA treated HCC cells were affected by MCC,the survival rate of HepG2,HepG2/Silence(-) cells was inhibited only at 48 h(0.505?0.015) compared to control groups untreated with MCC(0.824?0.322)(P

Objective To investigate the therapeutic effect of microencapsulated xenogenic hepatocytes transplantation on rat fulminant hepatic failure(FHF).Methods Isolated guinea pig hepatocytes were microencapsulated with alginate-chitosan-polyethyleneglycol(ACP).The microencapsulated hepatocytes(MCHC) were implanted into the peritoneal cavity of Wister rats 24 hrs before the FHF model,which was surgically induced by 90% hepatectomy.After 24hrs of the FHF,the serum ALT,total bitirubin(TB),blood suger(BG) and PT were determined,and the microscopic appearance,function and nember of the implanted microencapsulated hepatocytes were observed,as well as the survival time and life quality of the FHF rats. Results Compared with the control group,the rats in the MCHC implantation group showed milder symptoms of central nervous system and less serious ascites.The ALT,BS, TB,and PT in the MCHC implantation group were all improved significantly (P

Objectives:To determine if enteral nutrition can protect the gut barrier in patients with damaged hepatic function and draw the clinical significance from it. Methods:76 patients whose hepatic function was in Child B or C grade were randomly assigned in enteral nutrition group(EN,30 cases),total parenteral nutrition group(TPN,26 cases) and control group(CON,20 cases).They were given with different nutritional support. Nutritional parameter and hepatic function indexes were massured at the day before operation,5th day and 10th day after the operation respectively.The concentrations of lactulose(L) and Mannitl(M) were detected in the urines with pulsed electrochemical detection(HPLC PED) and the L/M ratio was calculated. Results:No significant differences of hepatic function changes were observed between EN group and TPN group.It was the earlist that EN group reached the positive nitrogon equilibrium among the 3 groups and the loss of body weight after operation was the lowest in EN group There wes no change in L/M ratio in EN group, but the change was significant in TPN group( P

In order to evaluate the value of angiographic diagnosis and interventional therapy in the patients with massive hemobilia, 10 patients with massive hemobilia were analyzed retrospectively from Sep. 1990 to Sep. 1999. All of them underwent emergency celiac and selective right or left hepatic artery angiography. The patients were diagnosed rapidly and treated successfully by embolization of the hepatic artery branch proximal to t he bleeding point, and hemorrhage was stopped immediately. Two out of 10 patients were embolized two times for bleeding again. All patients were alive and followed up for 3 months to 5 years. As a safe, reliable and mininally invasion method, selective hepatic artery angiography and embolization was proved to be an important diagnostic and therapeutic means for massive hemobilia, which had obvious advantages of minimally invasive reatment in unstable patient over surgery and should be the first choice for such patients.

Objective To assess the safety and efficacy of an olive oil-based lipid emulsion for parenteral nutrition in patients after hepatectomy.Methods Thirty-one postoperative patients with elective hepateetomy were randomized to receive isonitrogenous,isocalorie parenteral nutrition over 6 days after liver lobectomy(48-72hours)with either olive oil-based lipid emulsion(study group,n=15)or standard soybean oil emulsion(control group,n=16).The liver function and plasma protoins were assessed using peripheral venous blood collected before surgery,one day after surgery,and 7 days after surgery.The safety profiles of emulsion supports and postoperative rehabilitation were also assessed.Results The preoperative serum levels of total bilirubin,direct bilirubin,alanine amiotransferase,aspartate aminotransferase,alkaline phosphatase,total protein,albumin,and prealbumin were comparable between the two groups(all P>0.05).Although the Postoperative safety profile and liver function were not significantly different between two groups(all P>0.05),plasma total proteins,albumin,and prealbumin returned to the normal levels significantly faster in the study group than in control group[(57.57±9.84)g/L vs.(47.76±6.53)g/L,P=0.000;(31.29±3.11)g/L vs.(26.34±4.87)g/L,P=0.000;(0.188±0.059)g/L vs.(0.103±0.037)g/L,P=0.000]on the 7th Postoperative day,and the Postoperative hospital stay was also significantly shorter in the study group[(13.1±1.2)d vs.(15.2±1.1)d,P=0.041].The incidence of postoperative complications in study group and control group was 26.7%and 31.3%.respectively.Conclusions Treatment with the new olive oil-based lipid emulsion is weU tolerated in hepatectomy patients.It can speed up plasma proteins recovery and may shorten postoperative hospital stay,although it does not remarkably decrease the incidence of postoperative complications.

Objective To study the results using T-tube and retroperitoneal space drainage to manage perforating injury of the distal common bile duct(PIDC) during common bile duct(CBD) exploration.Methods We retrospectively analyzed the clinical data of 12 patients who were diagnosed to suffer from PIDC during CBD exploration from 2010 to 2012.Result All these 12 patients who received T-tube and retroperitoneal space drainage,gastrointestinal decompression,nutritional support and antibiotics recovered uneventfully.Conclusion Given that the CBD was unobstructed,T-tube and retroperitoneal space drainage was an good treatment for patients with PIDC.