Objective To study ST-segment deviation on predicting culprit artery or culprit lesion in acute myocardial infarction(AM/).Methods The study population included patients with AMI who subsequently underwent coronary angiography during hospitalization.Culprit artery and culprit parts were evaluated,and ST-segment characteristics were analyzed.Results A higher ST-segment elevation in leadⅢthan in leadⅡand ST-segment depression＞1 mm in lead aVL or lead I were highly sensitive(75%)and specific(100%)markers for right coronary artery-related AMI.ST-segment depression＞1 mm in leadⅡ,Ⅲand aVF was lowly sensitive(39%)and highly specific (93%)marker for the left anterior descending artery-related AMI.Conclusion The culprit artery or culprit lesion coutd be predicted by using electrocardiogram at admission.

Objective To study clinically the feasibility of early diagnosis of cardiac allograft vascularopathy (CAV) and chronic rejection. Methods A 13 year old female patient with dilated cardiomyopathy received orthotopic heart transplantation for advanced heart failure, and subsequent immunosuppressive therapy including cyclosporine, prednisone and mofetil, and a monthly close follow up. Coronary angiography and left ventricular endomyocardial biopsy (EMB) was performed 9 months after the operation. Results The clinical and follow up data of the case showed that cardiac or systemic nonspecific symptoms such as exertional chest discomfort, palpitation, fatigue or fever of unknown reasons were the first and ignorable clinical symptoms, and found disappeared after dosage addition of cyclinsporine, which indicated a early clinical manifestations of rejection or vasculopathy. While persistent sinus tachycardia on electrocardiogram, decreased left ventricular ejection fraction (from 64?% ～68?% down to 47?%～50?%), enlarged right atrial (from 32～ 41?mm up to 44?mm in diameter), thickened intraventricular septal ( 13?mm ), repetitive tricuspid regurgitation on echocardiography, increased white blood cells without infection were observed respectively. Coronary angiography showed a typical (type B) vasculopathy with diffuse concentric stenosis of mid and distal left anterior descending artery and its small braches, the distal diagonal, circumflex, and right coronary artery were also involved to some extent. Furthermore, early mild chronic rejection of grade I A was proved by pathologic study. Conclusion Close clinical observation and follow up, serial echocardiography and electrocardiogram, reaction to enhanced anti rejection, and other related laboratory methods could be analyzed and colligated for early noninvasive diagnosis of cardiac allograft vasculopathy and chronic rejection, while EMB and coronary angiography are still the precise diagnostic ways.

AIM To study whether nitric oxide preconditioning induce early protection of neonatal rat cardiomyocytes. METHODS Cultured neonatal rat cardiomyocytes were divided into 6 groups. ①Normal group;②NO group: SNAP(500 ?mol?L -1 ) was added to cell medium 1 h before lethal hypoxia/reoxygenation (HR) injury (6 h hypoxia and 3 h reoxygenation); ③HP group: Cells were cultured for 60min hypoxia followed by 30 min reoxygenation to form hypoxia preconditioning before lethal HR injury;④ L NAME+HP group: Nitric oxide synthase antagonist L NAME was added during HP stage;⑤ L Arg group: L arginine was added to cell medium 1h before lethal HR injury;⑥ L NAME+ L Arg group: Both L NAME and L Arg were added to cell medium 1 h before lethal HR injury;⑦H/R group: Cells underwent lethal HR injury without any treatment. Cardiomyocytes injury was detected by lactate dehydrogenase(LDH) activity and cell viability. RESULTS Nitric oxide preconditioning can protect cardiomyocytes by reducing LDH activity and improving cell viability ( P

BACKGROUND:With the social and economic development and aging, coronary heart disease has become the primary cause of death endangering human life and health, while cardiovascular intervention (especial y stenting) in the treatment of cardiovascular disease is at a growing status. OBJECTIVE:To describe the development, research progress and latest clinical application of heart interventional materials as wel as advantages and disadvantages of biodegradable stents, and meanwhile to prospect for its future development and improvement. METHODS:A computer-based online search was conducted in PubMed for English language publications from January 1999 to April 2014 using the key words of“stent, scaffold, bioabsorbable, bioresorbable, biodegradable, biocompatibility, material properties”in English. RESULTS AND CONCLUSION:The new ful y biodegradable stents are considered as another major progress in the cardiac intervention, which brings a new gospel for patients with coronary heart disease. In the initial stage of implantation, biodegradable stents exhibit the same mechanical support as bare metal stents;after implantation, anti-proliferative drugs are released to prevent thrombosis and restenosis, and the stent is completely degraded within a specified period, which significantly reduces the late-stage and very late-stage thrombosis and the risk of in-stent restenosis. However, long-term safety and efficacy of biodegradable stents need further studies. Innovation of stent design and materials science is the key to overcome the current shortcomings of biodegradable stents.

AIM: To study on the expansion of CD~(4+) T lymphocytes isolated from rats with colitis induced by 2,4,6-trinitrobenzene sulphonic acid (TNBS). METHODS: The numeration and protein expression of CD~(4+) T lymphocytes had been performed by immunohistological analysis, flow cytometry and Western-blot. RESULTS: The numeration of CD4+ T cells and the protein expression of CD~(4+) T lymphocytes increased significantly in rats with colitis induced by 2,4,6-trinitrobenzene sulphonic acid. CONCLUSION: Expansion of CD~(4+) T lymphocytes in TNBS-induced colitis provides us a simple and reducible animal model for research of the new target of therapeutic drug.

OBJECTIVE:To study the analgesic and anti-inflammatory actions of essential oil extracted from Radix Angelica Sinensis METHODS:The pharmacodynamic effects were obsersed in five experimental models of inflammation and pain,including:(1)mouse auricular edema induced by xylen;(2)increased vascular permeability induced by acetic acid in mice;(3)paw edema caused by albumen in rats;(4)granuloma caused by implanted cotton ball in rats;(5) writhe reaction induced by acetic acid in mice RESULTS:Essential oil from Angelica Sinensis could distinctively inhibit the inflammatory edema caused by various inflammatory agents and reduce the times of writhe induced by acetic acid in mice CONCLUSION:Essential oil from Angelica Sinensis has analgesic effect and could not only inhibit acute inflammation but also chronic ones in animals

OBJECTIVE:To estabilish a HPLC-MS method for determining enalapril in human plasma METHODS:Alprozalam was added into plasma sample as an internal standard,then supernate of the sample was extracted through solid-phase extration column,washed with methanol and detected by HPLC-MS method:column,ODS C18;mobile phase,methanol-0 01% formic acid(45∶55);flow rate,0 8ml/min;capillary voltage,3 81kV;cone voltage,39 0V The selected ion was determined by EST RESULTS:The calibration curve was linear within the range of 2 5～400ng/ml r=0 9 996,the recovery was 102 2%,RSDs of intra -day and inter-day were 4 0% and 5 4%,respectively CONCLUSION:The method is accurate and sensitive with no endogenous interference It can be applied to studying the pharmacokinetics and bioavailability of enalapril tablets in humans

Objective To explore the diagnostic significance of anti-cyclic citrullinated peptide antibody(anti-CCP)and the role it plays in the articular erosion in patients with rheumatoid arthritis(RA).Methods The diagnostic significance of anti-CCP?AKA and RF were evaluated respectively.Seventy-five RA patients were devided into group1(limited radiographic damage group)and group2(severe radiographic damage group).The distribution of anti-CCP,AKA and RF in patients of the two groups were investigated.A univariate analysis was used to determine whether anti-CCP?AKA?RF?ANA?ESR?CRP or cutaneous nodules plays a role on articular erosion in RA.To determine which has the best predictive value for severe radiographic da-mage,all variables were entered into a logistic regression model.Results The sensitivity and specificity of an-ti-CCP?AKA and RF were49%,94%;50%,93%;79%,67%respectively.When any two markers were combined,the specificity would be raised.The positive rate of these three markers were much higher in group2than that in group1.Anti-CCP had the highest OR(6.71)for articular erosion in RA.Logistic regression analysis showed a strong correlation between anti-CCP,AKA,CRP or cutaneous nodules and less favourable disease outcomes.Cut.aneous nodules had the strongest correlation with severe radiographic damage.Conclusion Anti-CCP is a satisfactory marker for RA.Diagnostic accuracy appears to be raised when anti-CCP combined with AKA and RF.Anti-CCP has a strong correlation with severe radiographic damage.To investigate multiple risk factors of articular erosion will be helpful to pridict the outcome of RA.

AIM: To evaluate the protection of Mn~(2+) on injury of human kidney tubular epithelial cell line induced by gentamicin, and investigate its principals. METHODS: Gentamicin was used to injury human kidney tubular epithelial cell line (HK-2) to produce model of kidney injury. MTT method was used to measure effect of Mn~(2+) on proliferation of cells after they were injured by gentamicin. The spectrophotometry method was used to observe the change of LDH,NAG and SOD effected by Mn~(2+). Ultrastructure was examined by electron microscopy. RESULTS: Although HK-2 cells were injured, Mn~(2+) promoted cells proliferation, decreased LDH activity and NAG content, increased SOD activity, and alleviated dilation of the mitochondria and crushing of lysosomes. CONCLUSION: Mn~(2+) can protect human kidney tubular epithelial cell line from injury induced by gentamicin. It may be in relation to inhibiting the oxidative injury, lightening dilation of the mitochondria, protecting the integrity of lysosomes and decrease the leakage of enzyme.