Objective To investigate the effect of radiation on the mitochondrion in adenocarcinoma A549 cells.Methods After A549 cells were irradiated with 0,0.5,3 or 8 Gy of 60Co γrays,mitochondrion membrane potential of A549 cells was detected by JC-1 probe,and ATP activity was measured by ATP kit in a chemiluminescence apparatus.The mitochondria DNA copy numbers was detected by real-time PCR assay.Results At 24 h after radiation,the mitochondrion membrane potential of A549 cells in all the irradiated groups changed significantly (F =243.44,P ＜ 0.05),among which 0.5 or 3 Gy of radiation resulted in a significant increase of mitochondrion membrane potential of A549 cells (t =-10.12,-5.59,P ＜ 0.05).However,the mitochondrion membrane potential of A549 cells exposed to 8 Gy of radiation decreased significantly 24 h after radiation (t =15.22,P ＜ 0.05).The mitochondrion membrane potential of A549 cells in all radiation groups returned to the normal level 48 h after radiation (F =10.36,P ＜ 0.05).24 h after radiation,the level of ATP in A549 cells significantly changed respectively(F =97.08,P ＜ 0.05),similar to the mitochondrion membrane potential.The ATP level in 0.5 and 3 Gy groups increased significantly (t =1.66,7.27,P ＜ 0.05),and the level of ATP in 8 Gy group decreased significantly (t =-8.24,P ＜ 0.05).Furthermore,48 h after both 0.5 and 3 Gy of radiation,the ATP content in A549 cells was still higher than that in untreated A549 cells (t =4.60,8.53,P ＜0.05).The mitochondria DNA copy numbers in A549 cells increased significantly in all the radiation groups (F =118.00,P ＜ 0.05).Compared with untreated A549 cells the mitochondria DNA copy numbers in A549 cells increased at 0.5 Gy by 12 times(t =0.02,P ＜0.05),and increased at 3 and 8 Gy by 7 and 10 times,respectively (t =9.68,15.10,P ＜ 0.05).Conclusions High dose of radiation resulted in the decrease of mitochondrion membrane potential of A549 cells,which subsequently affected the production of ATP.However,radiation with moderate and lower dose could lead to the compensatory increase of mitochondrion membrane potential of A549 cells,which promoted the production of ATP.The mitochondria DNA copy numbers compensatory would increase after A549 cells were exposed to radiation within 8 Gy.

Objective To investigate the association between air pollution and the daily number of outpatient visits for dermatitis in Beijing city, and to evaluate the sensitivity to air pollution in populations of different gender and age. Methods Time-series data on daily outpatient visits for dermatitis between April 2012 and April 2014 were collected from Air Force General Hospital of PLA. The daily average concentrations of sulfur dioxide (SO2), nitrogen dioxide(NO2), and particulate matters(PM2.5, PM10)were obtained from Beijing Municipal Environmental Monitoring Center, and routine meteorological data (including daily mean temperature, relative humidity and wind speed)were collected from the China Meteorological Data Sharing Service System. A generalized additive model was used to analyze the association between daily average concentrations of air pollutants and the number of daily outpatient visits for dermatitis. Results In single-pollutant models, an increase of 10 μg/m3 in daily concentrations of PM2.5, PM10, SO2, and NO2 was associated with the number of outpatient visits for dermatitis with the relative risk being 1.003 1 (95% CI, 1.001 6 - 1.004 5), 1.0025 (95% CI, 1.001 4 - 1.003 7), 1.0057 (95% CI, 1.001 5 - 1.009 9)and 1.009 7 (95% CI, 1.005 6 - 1.013 8)respectively. Similarly, multipollutant models showed that air pollutant concentrations were significantly associated with the daily number of outpatient visits for dermatitis. Distributed lag models showed that the effects of PM2.5, PM10 and NO2 on daily outpatient visits for dermatitis were mainly observed on the day of exposure, while the effect of SO2 increased along with the increment of lag days. Stratification analysis showed that people aged less than 60 years and females were relatively more sensitive to particulate matters (PM2.5, PM10), while NO2 affected all groups of people at the same degree, and SO2 mainly affected people aged 35 - 60 years. Conclusion Air pollutants PM2.5, PM10, SO2 and NO2 may all increase the risk of dermatitis in Beijing area.

Objective To investigate the association between concentrations of environmental air pollutants and the daily number of outpatient visits for urticaria in Beijing. Methods Time series analysis in combination with distributed lag non?linear model(DLNM)and Spearman′s rank correlation test was used to analyze the association between PM2.5 concentrations and the daily number of outpatient visits for urticaria. All effect estimates were expressed as a risk ratio(RR)for the daily number of outpatient visits with an interquartile range(IQR)increase of 82.8 μg/m3 in PM2.5. Results From January 1st 2013 to June 30th 2014, the daily average PM2.5 concentration, temperature, relative humidity and number of visits for urticaria were 89.3 μg/m3, 12.7 ℃, 51.9% and 27.3 respectively. Spearman′s rank correlation test showed that the daily average PM2.5 concentration and number of visits for urticaria were correlated with daily mean temperature(rs =-0.21, 0.36, respectively, P < 0.05)and relative humidity(rs = 0.49, 0.31, respectively, P < 0.05), but no correlation was observed between daily average PM2.5 concentrations and daily number of visits for urticaria(rs=0.01, P>0.05). There was no significant correlation between daily number of outpatient visits for urticaria and daily PM2.5 concentration lagged by 0-14 days(RR=1, 95%CI:0.89, 1.13). After potential confounding factors were adjusted, an 82.8μg/m3 increase in daily PM2.5 concentration at lag 0-20 days was significantly associated with a 22%(95%CI:3%, 46%)increase in daily number of outpatient visits for urticaria, suggesting that the lagged effect of PM2.5 was obviously increased. Conclusion The association between PM2.5 concentrations and the number of visits for urticaria is easily affected by meteorological factors and biological confounding factors, and relative humidity and mean temperature are both positively correlated with the number of visits for urticaria.

Diabetic macular edema is the leading cause of central vision loss and even blindness in diabetic retinopathy.Compared to FFA,OCT can obtain the high-resolution 3D image quickly,easily to reflect the details of the tissue and realize the quantitative measurement.As a novel technology,OCT angiography (OCTA) can display microvascular structure from different layers of retina and choroid,having its advantage of quantifying the vessel density and the lesion area.By detecting fundus morphology,quantifying and quantitating the retinal vessels and vessel density,the combination of OCT and OCTA could play a guiding role in diagnosis,classification,treatment and prognosis of diabetic macular edema.

Objective:To evaluate the safety and efficacy of endovascular interventional treatment of the intracranial vertebrobasilar trunk large aneurysms (VBTLAs) compared with conservative treatment.Methods:This is a prospective multi-center cohort study. From October 2012 to October 2018, a total of 69 patients with intracranial vertebrobasilar trunk large aneurysm (diameter>10 mm) from Henan Province People's Hospital and People's Liberation Army Rocket Medical Center were included in this study. Patients themselves chose either endovascular interventional therapy (interventional group) or conservative treatment (conservative group) after discussion with their doctors. The χ 2 test was used to compare the incidence of deaths, stroke, and all other serious adverse events including other site bleeding, myocardial infarction and others between the two groups. Results:A total of 69 patients were enrolled, of whom 51 patients were enrolled in interventional group, 18 patients underwent endovascular reconstructive therapy, 11 patients underwent deconstructive therapy, and 4 patients underwent conjunction interventional treatments. Eighteen patients were enrolled in conservative group, of whom 11 cases received simple risk factor control, 7 cases received antiplatelet and risk factors control. The proportions of hypertensive patients 94.4% (17/18) and giant aneurysms 50.0% (9/18) in the conservative group were higher than those in the surgery group 64.7% (33/51, χ 2=4.500, P=0.034), 19.6% (10/51, χ 2=4.730, P= 0.030).The incidence of all serious adverse events associated with protocol was 15.7% (8/51) in the interventional group and 44.4% (8/18) in the conservative group [risk ratio (RR) =0.353, 95% confidence intervals (CI): 0.156-0.801], and the difference was significant (χ 2=4.668, P=0.031). The incidence of fatal events associated with protocol was 2.0% (1/51) in the interventional group and 38.9% (7/18) in the conservative group (RR=0.050, 95%CI: 0.007-0.382), and the difference was significant (χ 2=14.281, P<0.001). The incidence of hemorrhage events associated with protocol was 2.0% (1/51) in the interventional group and 22.2% (4/18) in the conservative group (RR=0.088, 95%CI: 0.011-0.738), and the difference was significant (χ 2 =5.391, P=0.020). Follow-up imaging showed that the occlusion rate of aneurysms in 44 patients in the interventional group was 56.8% (25/44) after a median follow-up of 6 months. Imaging follow-up was obtained in 9 patients, whose occlusion rate of aneurysms was 0 and the median follow-up time was 12 months, in the conservative group. The difference was significant(χ 2 =7.534, P=0.006). Conclusion:Compared with conservative treatment, endovascular intervention of the intracranial VBTLAs has lower incidences of serious adverse events and death events.