Objective To investigate the rehabilitative effect of simulated family intervention on hospitalized depression patients.Methods One hundred depression patients in hospital were evenly randomized into the experiment group and control group:Both groups were treated with traditional antidepressant therapy and beside,the experiment group were taken care of by family members by way of simulated family intervention and the control group by isolated nursing intervention.The two groups were compared in therapeutic effect by survey using HAMD before intervention and 3 months after that.Result After 3 months of treatment,the score by HAMD in the experiment group was significantly lower than that in the control group(P<0.05).Conclusion The simulated family intervention by family members in hospital may be effective in improving the rehabilitation of depression patients and good for their resumption to the communities.

Objective To investigate the effect of exogenous murine interleukin-18 (IL-18) on early mouse collagen Ⅱ -induced arthritis (CIA). Methods Mice were injected intraperitoneallg IL-18 (0.2 μg/d) combination with IL-10 (0.1 μg/d), IL-4 (0.1 μg/d) and IL-12 (0.1 μg/d) daily for five days before the onset of CIA. The arthritis response was monitored visually by macroscopic scoring. Reverse transcription -polymerase chain reaction (RT-PCR) was employed to determine the mRNA expression of cytokine in patella with adjacent synovium in CIA mouse. Histology of knee was examined to assess the occurrence of cartilage destruction and bone erosion. Wilcoxon rank test was selected. Results IL-18/IL-4 treatment could slightly suppress the macroscopic score of arthritis, but a more pronounced amelioration was found in mice treated with the combination of IL-18 and IL-10 during early treatment. On 38 days after immunizatian macroscopic score in treated group (0.12±0.20) was significantly improves than in the control group (0.29±0.19, P<0.05). This resulted in both the suppression of macroscopic signs of inflammation and the reduction of cellular infiltrates in the synovial tissue, which provided the protection against cartilage destruction. Moreover, the expression of Thl cytokines [IL-18 (0.22±0.06), IL-12 (0.14±0.05)] and inflammatory cytokine [IL-6 (0.22±0.11)] was greatly inhibited both in the synovial tissue and in the articular cartilage in the treatment groups compared with those in the control groups (P<0.05). However, the mRNA levels of Th2 cytokines [IL-10 (6.35±0.12), IL-4 (3.57±0.13)] were up-regulated after IL-18/IL-10 treatment (P<0.05). Moreover, IL-18R (0.40±0.15) levels were down-regulated compared with those in the control group (P<0.05). T-bet mRNA levels were decreased in IL-18/IL-10 compared with the control group, and GATA-3 mRNA (5.71±0.11) levels were significantly higher than those in the control group (P<0.05). Conclusion Low dose IL-18/IL-10 treatment can inhibit Th1 cytokines expression and induce Th2 cytokines expression, which may be mediated not only by inhibiting Th1 responses through IL-18/IL-18R mechanism, but also by inducing anti-inflammatory mediators such as IL-10 and IL-4 through a GATA-3-dependent mechanism.

Objective To investigate the effect of exogenous murine interlukin-18 (mIL-18) on early and established murine coUagen-induced arthritis (CIA). Methods Mice were injected intraperitoneally with IL-18 (0.2 μg/mouse) daily for 5 days before or after the onset of CIA. The response was monitored visually by macroscopic scoring. Reverse transcription-polymerase chain reaction (RT-PCR) was employed to determine the mRNA expression of cytokines in patella with adjacent synovium in CIA mouse. Histolpgy of knee synovium was used to assess the occurrence of cartilage destruction and bone erosions. Results IL-18 alone had no effect on macroscopic score, occurrence of arthritis, advancement of histology on early stage of CIA. Moreover, expression of Th 1 cytokines and Th2 cytokines in the synovial tissue and" articular cartilage remained unchanged compared with the control group, however, a pronounced progression of histology was found in mice treated with IL-18 in estabhshed CIA. Forty-three days after immunization, the macroscopic score in the treated group (0.33±0.11 ) was significantly improved than in the control group (0.25±0.09) (P<0.05). Moreover, the mRNA levels of IL-10 and IL-18BP both in the synovial tissue and in the articular cartilage in the treated groups decreased significantly than those in the control groups (P<0.05). Conclusion Low dose mIL-18 alone has no effect on early stage CIA. But pronounced exacerbation is found in mice treated with IL-18 on established arthritis, which supports that IL-18 initiates this effect by inhibiting IL-10 and IL-18 BP.

With the increasing number of food safety problems caused by the abusing and misusing of plant hormones, it will be increasingly important to strengthen the trace detection of plant hormones in foods. In this study, benzyl methacrylate_methyl methacrylate_based amphiphilic polymer micelles were prepared, and a method for trace determination of five plant hormones was established by using these micelles. This method was rapid, sensitive and reproducible. Through optimization, the best experimental conditions were obtained as follows: 2 g/L polymer micelles, 50 mmol/L NaOH_H3BO3 buffer (pH 9. 2), 15 kV running voltage. Based on this novel method, naphthalene acetic acid in the solution of rooting powder was determined.

Objective To study influencing factors for giving up chemotherapy during initial treatment in elderly patients with multiple myeloma.Methods A total of 156 elderly patients diagnosed as multiple myeloma from 2000-2010 were retrospectively analyzed.And 123 young patients with multiple myeloma were selected as control group.The causes for giving up the chemotherapy and its related factors were recorded and analyzed.Results The rate of giving up the initial chemotherapy was higher in the elderly than in young patients [58.3％ (91/156) vs.35.0％ (43/123),P＜0.05].51.7％ of the elderly patients did not accept chemotherapy after diagnosis.The main factors for giving up the initial chemotherapy in elderly patients were critically ill,economic difficult and the low tolerance.However,the economic difficulty was the first cause in the young group.Conclusions The rate of giving up the chemotherapy in the elderly patients is high.Old age,severe conditions,economic difficulty,and low tolerance are the independent factors.

Objective To compare the efficacy of flurbiprofen axetil combined with fentanyl administered using different modes for postoperative analgesia.Methods This was a prospective,multicenter,randomized,double-blind,control,parallel-group study.ASA Ⅰ or Ⅱ patients,aged 14-91 yr,weighing 35-95 kg,scheduled for orthopedic,thoracic or hepatobiliary surgery under general anesthesia from January 2010 to October 2010,were randomly divided into A,B and C groups.The three groups received patient-controlled intravenous analgesia (PCIA) after surgery.In group A,flurbiprofen axetil 100 mg was injected immediately after the end of surgery and then PCIA was performed with fentanyl 1.0 mg in 100 ml of normal saline.In group B,PCIA was performed with flurbiprofen axetil 200 mg and fentanyl 0.6 mg in 100 ml of normal saline.In group C,flurbiprofen axetil 100 mg was injected immediately after the end of surgery and then PCIA was performed with flurbiprofen axetil 200 mg and fentanyl 0.6 mg in 100 ml of normal saline.The PCA pump was set up with a 2 ml bolus dose,a 10 min lockout interval and background infusion at a rate of 2 ml/h.VAS scores at rest and during activity and sedation score were recorded at the end of surgery and 4,8 and 24 h after surgery.The effective analgesia,excessive sedation,nausea and vomiting,dizziness,somnolence and respiratory depression were recorded within 24 h after surgery.Samples from the PCIA bump were taken to do microbe culture experiment at 24 and 48 h after surgery.Results Two thousand five hundred and ninety-six cases completed this trial (875 cases in group A,946 cases in group B and 775 cases in group C).Compared with group A,VAS scores at rest and during activity at the end of surgery and 4,8 and 24 h after surgery and sedation score were significantly decreased in group B,VAS scores at rest and during activity were significantly decreased at the end of surgery and 4 and 8 h after surgery and sedation scores were significantly increased at 4 and 8 h after surgery in group C,the rate of effective analgesia was increased in groups B and C,the incidence of excessive sedation was decreased in group B,while increased in group C,the incidence of postoperative nausea and vomiting was significantly decreased in groups B and C,and the incidence of postoperative dizziness was significantly decreased in group C (P ＜ 0.05).Compared with group B,no significant change was found in the VAS scores at rest and during activity,rate of effective analgesia,and incidences of nausea and vomiting,and somnolence (P ＞ 0.05),sedation scores were significantly increased at the end of operation and 4 and 8 h after surgery,the incidence of excessive sedation was increased,and the incidence of postoperative dizziness was decreased in group C (P ＜ 0.05).Neither bacterium nor fungus was found in the PCIA pump samples.Conclusion PCIA with flurbiprofen axetil 200 mg and fentanyl 0.6 mg (background infusion at a rate of 2 ml/h,2 ml bolus dose,10 min lockout interval) provides better efficacy and the occurrence of sides effects is low for the patients undergoing moderate or major operations.

Objective To investigate the effects of electroacupuncture (EA) pretreatment on the expression of phosphorylated signal transducer and activator of transcription 3 (pSTAT3) in cortical neurons in a rat model of focal cerebral ischemia/reperfusion (I/R) injury.Methods Forty-five adult male Sprague-Dawley rats,weighing 280-320 g,were randomly divided into 3 groups (n =15 each):sham operation group,I/R group and EA pretreatment group.Focal cerebral ischemia was induced by middle cerebral artery occlusion for 120 min,followed by 24 h of reperfusion.EA of Baihui acupoint lasting 30 min was performed and then the model of focal cerebral I/R was established 24 h later in EA group.Neurological function was assessed and scored at 24 h of reperfusion.The rats were then sacrificed and brains removed for detection of the cerebral infarct volume and expression of pSTAT3 (Ser727) in cortical neurons in ischemic penumbra by immunofluorescence and Western blot.Results Compared with sham operation group,the neurological function score was decreased,the infarct volume was increased,and the expression of pSTAT3 (Ser727) was up-regulated in groups I/R and EA (P ＜ 0.05).Compared with I/R group,neurological function score was increased,the infarct volume was decreased,and the expression of pSTAT3 (Ser727) was up-regulated in group EA (P ＜ 0.05).Conclusion EA pretreatment reduces focal cerebral I/R injury through up-regulating pSTAT3 expression in cortical neurons in rats.

AIM:To study the mechanism of foam floatation in the processing of Indigo Raturalis,and provide theory to support the industrialization of Indigo Raturalis processing by foam fioatation technique. METHODS: To study the critical problems of the processing of Indigo Raturalis by foam floatation technique: the change of particle size distribution after the processing of Indigo Raturalis;The surface activity of frothing materials in Indigo Raturalis;the distinction of the foam to absorb the Indigo particles and other particles(CaCO_3);the absorbability of the Indigo particles to absorb different surface active agents. RESULTS: 1.The Indigo particles were easily absorbed by the foam,but others(CaCO_3) were not;2.There were some frothing materials with good frothing capability in the alkaline system of Indigo Raturalis,3 In the alkaline system,the Indigo particles could be absorbed by the foam of strong acid surface active agents. CONCLUSION: In the alkaline system of Indigo Raturalis,with itself surface active agents,it is effective to process Indigo Raturalis by foam floatation technique.

Objective To study the process of foam flotation in separation of Indigo Naturalis in a continuous mode and to optimize the operational conditions.Methods Taking content and rate of recovery rate of indigo as index to investigate the single factors,such as the height of collecting region,the height of froth layer,flushing water rate,delivery rate,air flow rate,and aerating velocity,to study the effect of continuous foam floatation on Indigo Naturalis,and to optimize the process conditions finally.Results The flotation performance is good when the height of collecting region is 1.5 m,the height of froth layer is 30 cm,the delivery rate is 0.1 cm/s,the flushing water rate is 0.01 cm/s,the aerating velocity is 1.5 cm/s.The recovery rate of indigo is more than 75% and indgo content is over 5.0%.Conclusion Foam flotation technique is stable and can be used to the separation of Indigo Naturalis in a continuous mode.And this study is the foundation of semi-works production of Indigo Naturalis.

AIM:To optimize the flocculation-clarification process of Danshen aqueous extract.METHODS:Based on the single factor experiment,the turbidity of the index components danshensu,protecatechnic aldehyde concentration in the Danshen aqueous extract after flocculation were choose among the kinds and amount of flocculant and flocculation temperature.RESULTS:The turbidity of the chitosan-treated supernatant was lower than that of the ZTC1+1Ⅱ-treated ones,and this treatment can mitigate the membrane fouling better.CONCLUSION:Chitosan is superior to ZTC1+1Ⅱ as the flocculant of the Danshen aqueous extract.