Objective To investigate the effect of health education under Doctor-nurse-therapist Integrated Model on patients after lower limb amputation. Methods 70 patients after lower limb amputation were divided into experimental group (n=35) and control group (n=35). The experimental group received health education under the Doctor-nurse-therapist Integrated Model, while the control group received the routine health education. The success rate of health knowledge, satisfaction to nursing care, activities of daily living (ADL) and time stay in hospital were compared. Results The success rate of health knowledge, satisfaction to nursing care and score of ADL were more in the experimental group than the control group (P<0.05), while the time stay in hospital was less (P<0.01). Conclusion The Doctor-nurse-therapist Integrated Model can make the health education continuous and comprehensive, and it is more effective on patients with lower limb amputation.