Food allergy is a anaphylactic disease which is very common among children and its morbidity is rising in many countries.Food allergy could cause various diseases among different systems of the body,and it could also have a direct impact on patients' quality of life,even on life threatening.This article mainly focuses on the diagnosis technology of anaphylactic disease among children and the research progress of its treating methods.It will briefly introduce the risk factors and protective measures of anaphylactic disease among children,and also provide abundant theoretical foundation for clinicians to make diagnoses and give treatment correctly as well as to guide children's diet.

Objective To explore the effect of four-color personality analysis (FPA) in teaching of neurology nursing. Methods Fifty-five student nurses of 3-year courses were assigned into the control group from June 2011 to May 2012 , and another 55 students from June 2012 to May 2013 into the experiment group. For the teaching of neurology nursing, the traditional teaching methodology was used in the control group and FPA teaching methodology in the experiment group. The two groups were compared in terms of their internship coping capacity and critical thinking ability before and after the nursing internship. The evaluation from them over teaching effectiveness was analyzed. Results The experiment group gained significantly higher scores on ability in solving problems, asking for help and rationalization than the control group did (P<0.05), and significantly lower scores on self-reproach (P<0.05) at the end of the internship. The experiment group gained significantly higher scores on critical thinking ability, confidence and knowledge learning than the controls did (P<0.05). The student nurses in the experiment group all believed that the four-color personality analysis helped them understand themselves, better their personality, make them a adapt to the clinical settings better, and enhance their ability in communication. Conclusion FPA applied in the teaching of neurology nursing can effectively improve coping capacity and critical thinking ability of student nurses, help them better their personality and be more adaptive to different clinical environment and tasks, and ultimately improve the teaching quality.

Objective To observe the effect of valsartan on brian ultrastructure, Klotho gene and micro-inflammatory factor [intercellular adhesion molecule-1(ICAM-1) and vascular cell adhesion molecule-l(VCAM-1)] expression in spontaneously hypertensive rat models. Methods Ten male spontaneously hypertensive rats of 22 weeks age were selected and randomly divided into a hypertension group and a valsartan intervention group, while another 5 Wistar-kyoto rats were set as a normal contrast group. The brain ultrastructure of the 2 groups was observed by electron microscope. The expression of micro-inflammatory factor (ICAM-1 and VCAM-1)and Klotho gene was detected with RT-PCR, immunohistochemistry, and Western blot, respectively. Results The cerebral neuron damage of spontaneously hypertensive rats whose ultrastructure showed cell-pyknosis, chromatin margination and typical apoptotic body formation were alleviated after the intervention of valsartan. RT-PCR showed that the gene expression of Klotho increased while ICAM-1 and VCAM-1 decreased after valsartan intervention. Immunohistochemistry and Western blot also showed that the protein expression of Klotho increased, while ICAM-1 and VCAM-1 decreased after valsartan intervention. ConclusionValsartan can improve the brain ultrastructure of spontaneously hypertensive rats by increasing the expression of Klotho.

Objective: To explore the effect of application of standardized communication mode in doctor -nurse communication. Methods:Using stratified cluster sampling method, 96 nurses and 49 doctors from 10 nursing units were invited to participate in this study. The standardized communication mode training was carried out for nurses, which was implemented by communication guide and electronic medical interaction system. The doctors′satisfaction to nurses′job was evaluated before and after the implementation, and the nursing staffs′attitude toward standardized communication mode was investigated. Results:After 1 year, doctors′satisfaction to nurses′job was higher than that of before, the difference was statistically significant. Nursing staffs′acceptance toward standardized communication mode presented a high level. Conclusions:The standardized communication mode is beneficial to improve the doctor′s satisfaction to the nurses′work, especially in understanding of the disease condition, profes-sional knowledge and cooperation with the doctors. This communication mode is worthy popularizing in the clinical situation.

Objective To explore the changes of the Klotho protein expression in blood serum of hypertensive patients with brain harm,so as to investigate the incidence mechanism of brain harm caused by hypertension and the associated risk factors.Methods The research objects were recruited from the hypertensive in-patients and healthy individuals from health examination during September 2007 to March 2008 in our hospital.There were 56 hypertensive patients without syndrome,104 hypertensive patients with brain harm,and 30 normal control individuals,They all were marched in sex and age.Klotho protein concentration of blood serum,cholesterin,creatinine,blood glucose and blood pressure of these 3 groups were tested.And correlation analysis of the above factors and the patients' ages and hypertension medical records was performed.Results The Klotho protein concentration of blood serum in the normal control group was obviously higher than that of the hypertensive patients without syndrome and those with brain harm(P

Many studies have been conducted at home and abroad about carotid artery stenting to treat carotid artery stenosis. However, it is limited by the complications such as perioperative ischemia. Carotid artery stenting with cerebral protection devices can omit the procedure to block blood flow and can know the treatment condition through angiography. The results of comprehensive data show that carotid artery stenting is an efficient treatment technique, and can reduce the biocompatible responses of the host. In addition, carotid artery stenting with cerebral protection devices is a safe and effective therapy for high-riskcarotid artery atherosclerotic stenosis in the elderly.

Objective To investigate the dynamic changes and clinical significanes of TNF?α,IL?1,IL?10 and HMGB?1 in patients with tuberculosis. Methods Fifty?nine patients with active tuberculosis were enrolled in this study. The patients received the standard chemotherapy and the curative effect of the patient was evaluat ed according to the outcome classification in our country. The peripheral blood serum from the patients during the treatment and 12 healthy volunteers were collected. The concentrations of serum TNF?α,IL?1,IL?10 and HMGB?1 were detected by ELISA tests. Results Significant decreases of the concentrations of serum TNF?α,IL?1 and HMGB?1 were shown in patients from the initial treatment,to retreatment and extra?pulmonary tuberculosis (P<0.01),and the serum IL?10 was increased significantly (P<0.01) along with the chemotherapy and improvement of the disease. At the beginning of the treatment ,the levels of 4 kinds of inflammatory factors had no significant differences between the completed and uncompleted treatment patients (P>0.05). At the end of the treatment , the levels of serum TNF?α,IL?1 and HMGB?1 of the uncompleted treatment group were significantly higher than the normal levels (P<0.01) ,but the level of IL?10 was significantly lower than the normal level (P<0.01). Conclusion The dynamic testing of the inflammatory cytokines contributes to evaluation and judgement of the curative effect and the condition of patients with the active tuberculosis.

Objective To explore effects of comfort nursing on anxiety, depression and comfort of surgical patients.Methods Comfort nursing was done to 128 surgical patients, including physical comfort, psychological comfort and social comfort and self-designed questionnaire was made to investigate the comfort of patients during operation. When the patients were hospitalized and after operation, patients’physical states were evaluated with self-rating depression scale(SDS)and self-rating anxiety scale(SAS).Results The score of patients’comfort was (6.5±0.2)and the SDS and SAS were lower than that of patients when hospitalized with statistical difference(P<0.05).Conclusion Comfort nursing can reduce anxiety, depression and increase comfort of surgical patients.

Aim To investigate the effect of cobra venom metalloproteinase atrase A on human endothelial cell.Methods The effects of atrase A on the growth of HMEC were measured by MTT,SRB and morphological observation methods,respectively.After exposure to atrase A,IL-8,ICAM-1,MCP-1 and E-selectin released by HMEC were detected.Caspase-8 and caspase-3/7 were detected by fluorescent luminescence method.After intravenous injected atrase A to rats,the concentrations of ICAM-1,IL-8 and TNF-? in serum were measured.Results The results showed that atrase A(400,40 mg?L-1) significantly inhibited the growth of HMEC in low cell density.The microscopic examination revealed that atrase A detached the adherent HMEC.After exposure to atrase A,IL-8,ICAM-1 and MCP-1 released by HMEC were increased.Atrase A induced HMEC to express caspase-3/7 and caspase-8.After the administration of atrase A to rats,the concentrations of ICAM-1,IL-8 and TNF-? in serum were increased.There was no difference between the low-dose group and control group in our experiments.Conclusion Cobra venom metalloproteinase atrase A inhibits the growth of HMEC,and induces HMEC releasing inflammation mediators and apoptosis.

AIM: To investigate the clinical efficacy of trimebutine maleate combined with Morita therapy in the treatment of patients with irritable bowel syndrome (IBS). METHODS: 108 patients were randomly assigned to two groups. Trimebutine maleate group (n=54) was given trimebutine maleate 200 mg, po, tid, combined with Mortia therapy. The patients were analyzed by self rating anxiety scale (SAS) and self rating depression scale (SDS) before and after the treatment. Control group (n=54) was only given trimebutine maleate 200 mg, po, tid. The course was 4 weeks. RESULTS: The total efficacy rates were 87.04 % and 72.22 % in trimebutine maleate group and in control group, respectively. The therapeutic effects in trimebutine maleate group were better than those in the control group (P