Objective:EAE model of male and female Wistar rats was established respectively.The sex difference was evaluated.Methods:40 wistar rats were devided into male and female group (n=20).EAE model was established respectively in the two groups using Guinea pig spinal cord homogenate without PBS perfusion.30 days later,the spinal cord were taken and embedded in wax,and paraffin sections of the two groups were examined by histopathology.The differences in onset time,incidence rate,course of disease and neurologic score between the two groups were evaluated.Results:The onset time of female group was 13.67?3.50 days,its incidence rate was 60%.The onset time of male Wistar rat was 12.18?1.55 days,and its incidence rate was 85%.The feature of diseases course in female rat was relapse after recovery,but that in male rat was transient.The average clinical score of female group was 2.20?1.96,and that of male group was 3.46?1.61.Conclusion:Compared with female Wistar rat,the male had higher incidence rate and its diseases course was transient.The results of our study provided a useful foundation for further studies on the pathogenesis and the sex differences of multiple sclerosis.

Objective To detect the immunoregulation and clinical effect ofYupingfeng capsule combined with Seretide on patients with cough variant asthma (CVA).Methods CVA Patients were randomly divided into the Seretide control group (n=54) andYupingfeng capsule combined with Seretide group (n=54). Seretide group received inhaled Seretide. Combined traditional Chinese medicine group received Seretide and Yuping Feng capsule. Two groups were treated for 12 weeks. The IL-17, IL-10 and IL-6 expression was detected by ELISA analysis. The clinic effect rate and adverse events were compared.Results After treatment, compared with the Seretide group, the expression of IL-17 (18.72 ± 4.26 ng/mlvs. 26.17 ± 5.58 ng/ml;t=2.462,P<0.05) and IL-6 (21.58 ± 4.12 ng/mlvs. 30.66 ± 6.27 ng/ml;t=2.523,P<0.05) were significantly lower in combined traditional Chinese medicine group than that in Seretide group; and IL-10 (15.56 ± 2.74 ng/mlvs. 12.25 ± 2.81 ng/ml;t=2.244, P<0.05) was significantly higher in combined traditional Chinese medicine group. The daytime (1.12 ± 0.26 vs.1.42 ± 0.33,t=2.283) and night time cough score (1.24 ± 0.28vs. 1.52 ± 0.37,t=2.291) in combined traditional Chinese medicine group was significantly lower than that in Seretide group (P<0.05). The clinic effect rate (92.6%vs. 77.8%,χ2=2.438) in combined traditional Chinese medicine group was significantly higher than that in Seretide group (P=0.037).ConclusionYupingfengcapsule combined with Seretide can decrease IL-17 expression and increase IL-10 expression to inhibit inflammatory reaction in CVA patients, and showed significantly higher clinical effect rates.

Two γ-glutamyl-cysteine peptides (γ-GCPs ) , ( SC2 RC7 )-γ-L-glutamyl-S-allyl-L-cysteine ( 1 ) and ( SC2 RC7 )-γ-L-glutamyl-S-propyl-L-cysteine ( 2 ) have been isolated from fresh garlic scales using ion-exchange chromatography and pre-HPLC. Their molecular structures were identified by HPLC-MS, CD, 1 H NMR, 13 C NMR, specific rotation and confirmed by the corresponding standard compounds. The influence of exogenously adding 1 and 2 on the bioavailability of iron and zinc from food legume was examined with soybean and mung bean, in the level of 0. 01 mmol/5 g of legume respectively. The enhancing effect of the two γ-GCPs of compound 1 and 2 on bioaccessibility of iron was generally evidenced in the case of soybean ( from 1 . 88% to 6 . 73% and 4 . 42%) and mung bean ( from 2 . 52% to 12 . 04% and 9 . 38%) . The two γ-GCPs similarly enhanced the bioaccessibility of zinc from the food legume, in soybean ranging from 13. 37% to 23. 95% and 20. 58%, and in mung bean from 15. 98% to 28. 44% and 27. 05%. Thus, both compounds 1 and 2 obviously had a promoting influence on the bioavailability of iron and zinc from food legumes. These findings are of practical value in a food-based strategy to enhance the bioavailability of trace minerals for human health.

Objective To establish the optimum syndrome classification method by using the technology of modern TCM diagnosis and artificial intelligence analysis method for menopausal syndrome differentiation of TCM. Methods Diagnostic information of menopausal syndrome patients was collected and syndromes were classified according to TCM syndrome differentiation standard. Three kinds of common data mining classification algorithm, Bayesian network, K-nearest neighbors and support vector machine, were used for analysis on information data of the four methods of diagnosis of menopausal syndrome.Results The time, classification accuracy, coverage rate and margin curve of establishing TCM syndrome model by the three kinds of algorithm methods under the circumstances of same training and data. The influence of the number of training samples of 3 kinds of algorithm methods was analyzed, and the model established by the three kinds of algorithms was evaluated.Conclusion Bayesian network algorithm is better than the other two methods in the menopausal syndrome classification effect.

Objective To investigate the effects of the laparoscopic surgery and open surgery on the postoperative intestinal function recovery time in elderly patients with colorectal cancer(CRC).Methods 66 elderly patients with CRC and treated in the 2nd People's Hospital of Kunming from January 2021to July 2022 were chosen and divided into the control group(n = 33)and the study group(n = 33)according to different operation ways.And the postoperative bowel function recovery time,radical,complications and surgical index were compared between the two groups.Results The recovery time of intestinal function in the study group was significantly shorter than that in the control group(P<0.05),but there was no significant difference in radical indicators between the two groups(P>0.05).The incidence of complications in the study group was lower than that in the control group,and the difference was statistically significant(P<0.05).The operation time of the study group was longer than that of the control group and there was the statistically significant difference(P<0.05),while the bleeding was less than that of the control group and there was the statistically significant difference(P<0.05).Conclusion Compared with the open surgery,laparoscopic surgery can more effectively shorten the recovery time of postoperative intestinal function,reduce the incidence of complications and intraoperative blood loss in elderly patients with CRC.Although there is no significant difference between two kinds of operation mode in terms of radical,it should be considered when it is applied in the specific operation time and other factors.