Objective To determine the levels of radiation-induced expressions of HIF-1α and Survivin in three human lymphoma cell lines with or without HIF-1a specific antagonist or HIF-1a siRNA transfection and to explore the possible mechanism of cell radioresistance.Methods Namalwa,Ramos,Raji cells were irradiated with X-rays with or without the combination of echinomycin (EC),a HIF-1α specific inhibitor,or transfection of HIF-1α siRNA.Then the expression levels of HIF-1α and Survivin proteins and mRNAs were determined by Western blot and real-time PCR,respectively.Results After irradiation,the expression level of HIF-1α protein was up-regulated at 10-20 h followed by up-regulation of survivin protein at 24 h (t =7.53-31.31,P ＜ 0.01 ) in three lymphoma cell lines.Radiation-induced up-expression of survivin protein was significantly reduced by either HIF-1α inhibition or EC treatment (t =7.21-32.81,P ＜0.01 ).However,radiation failed to induce up-erpression of survivin at both mRNA and protein levels after transfection with HIF-1α siRNA.Conclusion HIF-1α-Survivin pathway is activated in human lymphoma cells after radiation,which might be involved in the cell radioresistance.

Objective To investigate the relation of resistant index and function of transposited ovaries following radical hysterectomy in young women with early stage of cervical cancer.Methods A total of 22 young women(aged from 26 to 40 years old)with early stage of cervical cancer(FIGO Ⅰ a or Ⅱ a)were studied from Aug.1997 to Sep.2004 in the Affiliated Hospital of Inner Mongolia Medical Collage.Ovarian transposition was performed at the time of radical hysterectomy and pelvic lymphadenectomy in 12 cases who received postoperative radiotherapy;the rest 10 cases did not receive postoperative radiotherapy.Ovarian function was evaluated by serum FSH,LH,E_2 and B-ultrasonography in all of the cases.Results There was a statistically significant difference between the radiotherapy group and the non-radiotherapy group in serum E_2,FSH,LH levels and RI at the 24th month postoperatively.RI had a negative correlation with E_2 and positive correlation with FSH.Conclusion Ovarian transplantation can preserve the endocrine function of ovary for young women with cervical cancer.RI has a negative correlation with E_2 and positive correlation with FSH.RI is the important index of supervising ovarian function.Postoperative radiotherapy can influence RI and ovarian function.

Approaching an advantage in TCM and Chinese herbs, comparing the difference between them in many ways. TCM has macroscopic advantage and microcosmic disadvantage. Point out that only the theory of TCM be respected, the advantages of TCM can be kept.

Anti-N-methyl-D-aspartate receptor encephalitis (Anti-NMDAR-encephalitis) in children is a type of autoimmune encephalitis associated with NMDA receptor.Its etiology and clinical manifestations are specific in children.This disease is associated with teratoma more in adult than in children.However,its etiology is more associated with infection and heredity in children.The pathogenesis is that the anti-NMDAR-antibody can combine with NMDAR of neurons selectively and reversibly.The clinical manifestations include psychiatric syndrome,language,motor,sleep and autonomic nervous disorders.Tumor resection and immtmotherapy are chief treatments.This disorder is often misdiagnosed as it is rarely reported in children.It is valuable to make early diagnosis and treatment.This paper reviews the mechanism,clinical features,diagnosis and treatment of antiNMDAR-encephalitis of children.

BACKGROUND:Lumbar paravertebral muscles play an important role for lumbar spinal stability. Increasing studies emphasize on the change of paravertebral muscles in patients with lumbar degenerative disease and therefore have important clinical significance for the rehabilitation training of paravertebral muscle in patients with lumbar degenerative disease. ＜br> OBJECTIVE:To review the research progress of the change of paravertebral muscle in patients with lumbar degenerative disease and to look into the future development prospects. ＜br> METHODS:A computer-based online search of PubMed and WANFANG DATA between 1986 and 2014 was performed with the key words“lumbar vertebra, paravertebral muscle, psoas major, multifidus muscle, cross sectional area, muscle atrophy, fatty infiltration, muscle biopsy, MRI, electromyography”in English and Chinese, respectively. The clinical and experimental studies addressing the changes of paravertebral muscle in patients with lumbar degenerative disease were included. According to the inclusion and exclusion criteria, 48 literatures were included into the final analysis, 8 Chinese and 40 English. ＜br> RESULTS AND CONCLUSION:The change of paravertebral muscle in patients with lumbar degenerative disease includes muscle atrophy and fatty infiltration. The changes of paravertebral muscles are often detected with B-ultrasound, CT, MRI, EMG and muscle biopsy. Due to the differences of sample size, age and study method, there are different results, even conflicting. So some limitations of sample size, age and study method are needed to obtain accurate results. In addition, further studies wil focus on the verification of which nerve specifical y dominants lumbar paravertebral muscles.

[Objective] Via an empirical survey, this study is aimed to explore the imparting and mastering of Chinese cultural knowledge in China ’s present col ege English teaching, so as to offer some effective strategies for dealing with“Chinese culture aphasia”in current col ege English teaching in China. [Methods] Resorting to the research methodology of tests, questionnaires and interviews, it carries out an investigation of a number of col ege students and English teachers in Zhejiang Province into their mastering of Chinese cultural knowledge and its conveying in English as wel as the curriculum design of their col eges or departments. The relevant data derived from the research are analyzed qualitatively and quantitatively. [Results] The subjects al demonstrate the phenomenon of“Chinese culture aphasia”concerning both its comprehension and its English translation to varying degrees. Besides, cross-cultural commu-nication is twofold, so it is indispensible for learners to study simultaneously their native culture,which is a solid cornerstone and the crucial foundation for foreign language learning, but has been seriously deficient in col eges’curriculum design and students’textbooks; integrating Chinese as one of the com-pulsory courses and establishing corresponding examining and evaluating systems incorporating formative assessment and summative assessment, are sure to cope with“Chinese culture aphasia”in present col ege English teaching in China effectively. [Conclusion] The research and solution above are expected to shed light on teaching syl abus formulations and curriculum designs, enhance students ’humanistic quality and their communication capabilities in reality, and consequently popularize the excellent Chinese culture.

Procalcitonin (PCT)is a biomarker of early bacterial infection,and it can reflect severity of infection,and regarded as a prognostic factor.Community acquired pneumonia(CAP) is the main mortality and morbidity cause of children under 5 years old.The diagnosis of CAP mainly depends on clinical symptoms and chest radioactivity and regular blood test,and absent of specific indications,and mainly depends on clinicians experiences.The diagnosis and prognosis values in children CAP have been proved by many clinical trials.This paper reviews the clinical value of PCT on children CAP.

Hematopoietic stem cell transplantation ( HSCT) technology has been widely used to cure some high-risk hematologic diseases and malignancies, with the rapid development of hematology and transplantation immunology, especially human leukocyte antigen matching technology. It might play an important role in biological therapy and gene therapy.

Objective To explore the influence of Gui piperazine strange maleate on the high sensitive C-reactive protein(hs-CRP) and S100B in the serum of patients with acute cerebral infarction.Methods 90 patients with acute cerebral infarction were randomly divided into two groups,the control group(45 cases) and the treatment group (45 cases).The patients in the control group were treated through the conventional treatment,while the patients in the treatment group were treated plus Gui piperazine strange maleate.They were treated for fourteen days.The hs-CRP and S100B in the serum were detected before and after treatment.Results The total effective rates were 93.3％ in the treatment group and 84.4％ in the control group.There was a significant difference between two groups(x2 =4.0125,P ＜ 0.05).The hs-CRP and S100B were decreased after treatment in both groups (all P ＜ 0.05),and there was a significant difference between two groups after treatment (all P ＜ 0.05).Conclusion Gui piperazine strange maleate can improve acute cerebral infarction through mitigating inflammation.

Mandibular teeth and sustentacular tissues extracted from a healthy adult were treated using CT scanning to obtain two-dimensional image, and then a three-dimensional image was established using computer reconstruction technique. Simultaneously, a three-dimensional finite element model of rigid fixed bridge was established in abutments with mandibular 56 deletion. A fixing load of 100 N was vertically and horizontally loaded on the middle site of bridge, and the abutments were grouped according to the proportion of alveolar bone absorption of 5% sub-interval to analyze the effects of 0 50% alveolar bone absorption on stress distribution of rigid fixed bridge. When the alveolar bone absorption was zero or less than 10%, the stress of rigid fixed bridge was evenly distributed on abutments with the vertical load of 4 7 MPa and horizontal load of 6 9 MPa; when the alveolar bone absorption was greater than 10%, the stress of rigid fixed bridge was concentrated on the abutments with the vertical load of 9-16 MPa and horizontal load of 14-23 MPa. This suggested hthat alveolar bone absorption had great effect on stress distribution of rigid fixed bridge in abutments with mandibular 56 deletion, namely a little absorption would cause the changes of stress distribution.