Objective To investigate the relation of resistant index and function of transposited ovaries following radical hysterectomy in young women with early stage of cervical cancer.Methods A total of 22 young women(aged from 26 to 40 years old)with early stage of cervical cancer(FIGO Ⅰ a or Ⅱ a)were studied from Aug.1997 to Sep.2004 in the Affiliated Hospital of Inner Mongolia Medical Collage.Ovarian transposition was performed at the time of radical hysterectomy and pelvic lymphadenectomy in 12 cases who received postoperative radiotherapy;the rest 10 cases did not receive postoperative radiotherapy.Ovarian function was evaluated by serum FSH,LH,E_2 and B-ultrasonography in all of the cases.Results There was a statistically significant difference between the radiotherapy group and the non-radiotherapy group in serum E_2,FSH,LH levels and RI at the 24th month postoperatively.RI had a negative correlation with E_2 and positive correlation with FSH.Conclusion Ovarian transplantation can preserve the endocrine function of ovary for young women with cervical cancer.RI has a negative correlation with E_2 and positive correlation with FSH.RI is the important index of supervising ovarian function.Postoperative radiotherapy can influence RI and ovarian function.

Approaching an advantage in TCM and Chinese herbs, comparing the difference between them in many ways. TCM has macroscopic advantage and microcosmic disadvantage. Point out that only the theory of TCM be respected, the advantages of TCM can be kept.

Objective To determine the levels of radiation-induced expressions of HIF-1α and Survivin in three human lymphoma cell lines with or without HIF-1a specific antagonist or HIF-1a siRNA transfection and to explore the possible mechanism of cell radioresistance.Methods Namalwa,Ramos,Raji cells were irradiated with X-rays with or without the combination of echinomycin (EC),a HIF-1α specific inhibitor,or transfection of HIF-1α siRNA.Then the expression levels of HIF-1α and Survivin proteins and mRNAs were determined by Western blot and real-time PCR,respectively.Results After irradiation,the expression level of HIF-1α protein was up-regulated at 10-20 h followed by up-regulation of survivin protein at 24 h (t =7.53-31.31,P ＜ 0.01 ) in three lymphoma cell lines.Radiation-induced up-expression of survivin protein was significantly reduced by either HIF-1α inhibition or EC treatment (t =7.21-32.81,P ＜0.01 ).However,radiation failed to induce up-erpression of survivin at both mRNA and protein levels after transfection with HIF-1α siRNA.Conclusion HIF-1α-Survivin pathway is activated in human lymphoma cells after radiation,which might be involved in the cell radioresistance.

Animals ; Humans ; Learning ; drug effects ; Memory ; drug effects ; Metals ; toxicity ; Nervous System ; drug effects

Adult ; Female ; Humans ; Phenotype ; Polycystic Ovary Syndrome ; diagnosis ; epidemiology ; ethnology ; genetics

Precocious puberty in children begins with the increased pulsatile secretion of the gonadotrophin-releasing hormone(GnRH)from hypothalamic.Recently,KISS1-GPR54 Was found to be a key factor to regulate the secretion of GnRH and the onset of puberty.Kisspeptin interacts with its receptor GPR54.which expressed on the hypothalamic GnRH neurons.and affects GnRH pulsatile release and the onset of puberty.GPR54 gene mutation causes the incidence of GnRH-dependent precocious puberty.

Objective To observe air disinfection efficacy of ultraviolet ray,ozone disinfector,and circulating air disinfector in outpatient blood collection room.Methods Air in outpatient blood collection room was disinfected by ultraviolet ray,ozone disinfector,and circulating air disinfector,air specimen was taken before and after air disinfection,as well as after 1,2,3,and 4 hour working condition of staff,efficacy of 3 disinfection methods were compared.Results Under static condition,there was no significant difference in average colony forming unit(CFU) among 3 disinfection methods(P =0.317),the average CFU in air which disinfected by ultraviolet ray and ozone disinfector increased rapidly after the entry of personnel,the number of air colony did not meet the requirements of category Ⅲ environmental sanitation standard after staff worked for 2 hours;circulating air disinfector could continuously disinfect after the entry of personnel,the average CFU met the requirement of category Ⅲ environmental sanitation standard after staff worked for 1,2,3,and 4 hours;there were significant difference in the CFU among different disinfection methods(F =211.00,P＜0.001),there were significant difference in CFU at different sampling time(F =272.95,P＜ 0.001).Conclusion Circulating air disinfector can meet the requirements of category Ⅲ environmental sanitation standard during the working condition of staff,and is suitable for air disinfection in outpatient blood collection room.

0.05).Conclusion:The root caries among aged people in Chongqing is prevalent.The prevalence of caries is high but the treatment rate is lower.The preventive measure of oral disease should be enhanced.

Objective:To observe the effects of prolonged transient acidosis on cardiac hemodynamics in aged rat heart after reperfusion injury.Methods:60 isolated SD rats hearts(≥18 months)perfused by Langendorff were randomly divided into 6 groups(n=10):(1)normal contro1(C)group;(2)reperfusion injury(I/R)group;(3)ischemic postconditioning(IPO)group;(4)acidosis(H+)group;(5)postconditioning+alkalotic(IPO+OH-)group;(6)alkalotic(OH-)group.The direct effects of prolonged transient acidosis on myocardiac contractility were observed by a langendorff apparatus through the hemodynamics parameters including heart rate(HR),left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),and ?dp/dtmax.Results:The LVEDP were increased and HR,LVSP,?dp/dtmax were decreased in I/R group,IPO group,H+ group,IPO+OH-group and OH-group compared with those in C group(P

Objective:To evaluate the ability of the cooperative protection of the Strep to coccus pneumoniae multivalent DNA vaccine containing lytA gene(N-acetylmuramoyl-L-alanine amidase,LytA)and pspA'gene(N fragment of pneumococcal surface protein A,PspA)against intraperitoneal Streptococcus pneumoniae D39 infection.Methods The genomic DNA of the standard Streptococcus pneumoniae strain D39 was isolated.LytA gene fragment was amplified by polymerase chain reaction(PCR)and cloned into the eukaryotic expression vector pcDNA3.1(+),which was transfected into BHK-21 cells using Lipofectamine TM2000,and then the expression of LytA protein was detected with Western-blot.Furthermore,three formats of recombinant plasmid DNA vaccines(pcDNA3.1-LytA+pcDNA3.1-PspA',pcDNA3.1-PspA',pcDNA3.1-LytA)were used to inject intramuscularly BALB/c mice,with pcDNA3.1(+)plasmid and PBS as controls.The levels of antibodies against PspA'and LytA proteins were checked.Live times of intraperitoneal infection mice by Streptococcus pneumoniae D39 in BALB/c models were analysis.Results:The three DNA vaccines all producted high levels serum special IgG antibodies.The anti-LytA antibodies level elicited by the DNA vaccine pcDNA3.1-LytA + pcDNA3.1-PspA'was higher than the DNA vaccine pcDNA3.1-LytA(P0.05).The result of the effect on intraperitoneal infection by Streptococcus pneumoniae D39 in BALB/c mice models showed the median live times in pcDNA3.1-LytA + pcDNA3.1-PspA'group was longer than pcDNA3.1-LytA group and the control groups(P0.05).Conclusion:The multivalent DNA vaccine of Streptococcus pneumoniae containing LytA gene and pspA gene had no the higher performance protection than the DNA vaccine only containing pspA gene against Streptococcus pneumoniae.It is should be further evaluated that LytA gene is concluded as the one of advantages candidate antigens of the Streptococcus pneumoniae multivalent DNA vaccine.