Objective To study the relationship between serum lactadherin (MFG-E8),sialic acid (SA) level and coronary heart disease (CHD) occurrence and development.Methods 166 patients in our hospital were selected from June 2014 to November 2015,and then according to the result of coronary angiography divide the subjects into control group (n=56,the result of coronary angiography completely normal) and CHD group,including stable angina 48 cases (StA subgroup),unstable angina 30 cases (UA subgroup) and acute myocardial infarction 32 cases (AMI subgroup).The serum level of MFG-E8 was detected by ELISA method,the serum level of SA,hs-CRP and lipid index were detected by biochemical analysis.Gensini score showed the severity of coronary stenosis.The correlation analysis between detecting parameters was carried out by Spearman method.Results Compared with the control group,the patients with CHD have a higher level of serum SA and a lower level of MFG-E8 (both P<0.05).The AMI subgroup has a lower level of serum MFG-E8 than StA and UA subgroups,and the AMI subgroup has a higher level of serum SA than StA and UA subgroups (all P<0.05).The correlation analysis showed that the serum SA has a positively correlation with the level of TG,hs-CRP,and Gensini score,and has a negatively correlated with the level of MFG-E8 (all P<0.05).The MFG-E8 has a negatively correlated with the level of TG,hs-CRP,and Gensini score (all P<0.05).Conclusion MFG-E8 and SA play an important role in the development of CHD,and combined detection of serum MFG-E8 and SA may be helpful in the clinical diagnosis and risk assessment of patients with CHD.

Objective To evaluate the safety and effect of extraperitoneal laparoscopic radical prostatectomy for high-risk prostate cancer. Methods From January 2012 to August 2015, 49 patients diagnosed high-risk prostate cancer underwent extraperitoneal laparoscopic radical prostatectomy with 'sandwich' urethra reconstruction. Results None converted to open surgery and the mean operative time was (2.15 ±0.29) h, mean intraoperative blood loss was (60.25 ± 20.29) ml. No rectal injury was observed. The patients were ambulant 1 to 2 days postoperatively. Pelvic lymph nodes metastasis was found in 5 cases. Positive margin was found in 13 cases. Mild urinary incontinence oc-curred in 3 cases. Urethral stenosis occurred in 2 cases. Biochemical relapses occurred on 5 cases during the follow-ing period of 1~43 months for 46 cases. Conclusions Extraperitoneal laparoscopic radical prostatectomy is safe and effective in treatment of high-risk prostate cancer. The very key is to master the anatomy of prostate and laparoscop-ic techniques.

Objective To analyze the diagnosis of variant acute myocardial infarction.Methods Clinical datas were retrospectively analyzed in 27 cases with variant acute myocardial infarction.Results 27 patients were diagnosed with myodial infarction by ECG and myocardial enzymea.Conclusion Variant acute myocardial infarction is often misdiagnosed as acute abdomen,cerebral aarterial infarction the disease of neck.but its clinical manifestation especially ECG and myocardial enzymes are changeable,and it needs full analysis,early diagnosis and therapy.

Objective To investigate the expression of human telomerase reverse transcriptase(hTRT) in meningiomas,the correlation with malignancy grade and the prognostic significance of hTRT activity.Methods The expression of hTRT and proliferating cell nuclear antigen(PCNA) in 43 meningiomas was examined by immunohistochemical technique.Postive rates of hTRT between histopathologically different grades were analyzed,and so did between initial detected case group and recurrent case group.The labelling indexes of PCNA between hTRT-positive group and hTRT-negative group were also analysed.Results Of 43 meningiomas examined,19(44%) demonstrated the expression of hTRT.hTRT was present in 3 of 26 benign meningiomas(11 5%),10 of 11 atypical meningiomas(90%) and 6 of 6 malignant meningiomas(100%),respectively(P

Objective To explore the effect of Periplaneta americana reversing multiple drug resistance (MDR) of hepatocellular carcinoma in vitro. Methods Drug resistance of Hepatocellular carcinoma cell line HepG2/ADM was established by Adriamycin ( ADM ) increasing concentration gradient method;determined cell growth curve, doubling time change using Trypan blue staining method;detected sensitivity of 4 kinds of chemotherapeutic drugs of HepG2 and HepG2/ADM by methyl thiazolyl tetrazolium method; detected the non toxic dose of Periplaneta americana CⅡ-3 and the skim cream of HepG2/ADM by MTT method; detected the inhibition rate differences of CⅡ-3, skim cream, ADM, CⅡ-3 and ADM, skim cream and ADM of hepatocellular carcinoma drug resistance cell line HepG2/ADM by MTT method.Results Drug-resistant cell line doubling time was extended obviously compared with parent cells,HepG2/ADM showed resistance to variety of chemotherapeutic drugs, ADM resistant index was especially high,that was 15.25 times.When the concentration of CⅡ-3 and skim cream were 28.76 and 45.08μg/mL respectively, the growth inhibition rate of HepG2 and HepG2/ADM two cell lines were<10%,no obvious toxic effect.The inhibition rate of the drug-resistant cell of 7.5,15,30μg/mL concentration CⅡ-3 joint with ADM concentration (1/2 IC50 ) were (24.52 ±2.05)%,( 34.78 ±3.08)%,( 45.09 ±3.56)% respectively; The inhibition rate of the drug-resistant cell of 12.5,25,50μg/mL concentration skimmed cream joint with ADM concentration (1/2 IC50 ) were (19.28 ±2.56)%,(35.08 ±2.13)%,(43.85 ±3.05)%respectively.Conclusion The cell line HepG2/ADM established has basic multidrug-resistant biological characteristics.Periplaneta americana extract can inhibit the growth of HepG2/ADM,and has good effect to reverse drug resistance at the same time.

Adult ; Crown Lengthening ; Dental Restoration, Permanent ; Esthetics, Dental ; Female ; Humans ; Incisor ; Malocclusion ; therapy

Integrating visualization toolkit and the capability of interaction, bidirectional communication and graphics rendering which provided by HTML5, we explored and experimented on the feasibility of remote medical image reconstruction and interaction in pure Web. We prompted server-centric method which did not need to download the big medical data to local connections and avoided considering network transmission pressure and the three-dimensional (3D) rendering capability of client hardware. The method integrated remote medical image reconstruction and interaction into Web seamlessly, which was applicable to lower-end computers and mobile devices. Finally, we tested this method in the Internet and achieved real-time effects. This Web-based 3D reconstruction and interaction method, which crosses over internet terminals and performance limited devices, may be useful for remote medical assistant.
Diagnostic Imaging ; Image Processing, Computer-Assisted ; Imaging, Three-Dimensional ; Internet ; Software

Objective To explore the relationship between serum thyroid-stimulating hormone ( TSH) and dyslipidemia. Methods A total of 1 512 inhabitants in Zhaiji community of Guiyang urban areas were investigated from November 2009 to February 2010 by adopting stratified cluster sampling method, and 1 428 subjects were included in the present study. All subjects were asked to fulfill the questionnaires and to pass a physical examination. The vein blood samples were taken for determination of serum TSH, fasting plasma glucose (FPG), insulin, as well as blood lipid levels. Results Triglyceride ( TG ) , total cholesterol, high density lipoprotein cholesterol, and low density lipoprotein cholesterol levels were significantly increased in woman with subclinical hypothyroidism(SCH) compared to those with euthyroid(all P<0. 05). However, there was no significant difference in man between two groups(P>0. 05). The incidences of hypertriglyceridemia in the euthyroid group from quartile 1 toquartile4ofTSHwere33.3%,35.5%,39.7%,and44.0% respectively(P<0.05). SerumTGlevelwas increased with TSH in woman and total subjects after adjustment for age, sex, body mass index ( BMI) , waist-hip ratio (WHR), and homeostasis model assessment for insulin resistance index(HOMA-IR, P<0. 05). Serum TSH levels in the total sample were positively associated with TG after adjustment for age, sex, BMI, WHR, and HOMA-IR (β=0. 088,P<0. 05). Conclusion Higher serum TSH levels even within normal range seems to be a risk factor of dyslipidemia.

Objective To investigate the prevalence and the risk factors of dyslipidemia in residents aged 40 to 80 years in Guiyang. Methods A total of 10 022 adults (2 694 men and 7 328 women) aged 40 and over were selected from Yunyan community in Guiyang urban area for this population based cross-sectional study. Data were obtained by questionnaire survey, physical examination, fasting blood glucose, and lipid measurements. The association between influential factors and dyslipidemia was analyzed by logistic regression analysis. Results The standardized prevalence of dyslipidemia in residents aged 40 years and over in Guiyang was 46. 9%, with low high-densitylipoproteincholesterol(HDL-C)29.0%,hypertriglyceridemia19.3%,hightotalcholesterol(TC)9.3%, andhighlow-densitylipoproteincholesterol(LDL-C)4.3%. Logisticregressionanalysisindicatedthatage,gender, smoking, and alcohol abuse were the risk factors of high TC and high LDL-C. Abdominal obesity was a main risk factor for both hypertriglyceridemia and low HDL-C. Conclusion The prevalence of dyslipidemia of middle-aged and aged residents in urban districts of Guiyang was on the national average level. Hypertriglyceridemia and low HDL-C were the most common types. Age and abdominal obesity were important risk factors of dyslipidemia.

HepG2 cells were pre-incubated with insulin (Ins 0,1,0. 1,0.01 μol/L) and dexamethasone ( Dex 0,3,0. 3,0.03 μol/L) alone or together for 24 h to induce insulin resistance (IR) in vitro, the resistant level was estimated by glucose consumption, the optimal model of insulin resitance was chosen, and at the same time its lasting time of resistance was determined. In order to investigate the effects and mechanisms of mifepristone on in sulin-resistant HepG2 cells induced by insulin and dexamethasone, mifepristone and pioglitazone were adminis tered 24 h after the optimal model of insulin-resistant HepG2 cells was established. The glucose consumption, in tracellular concentrations of glucose, glycogen, ATP, and free fatty acid (FFA) in each group were detected. The expression of InsR-mRNA and GR-mRNA was detected by semi-quantitative reverse transcription and polymerase chain reaction (SqRT-PCR). Results revealed that pretreatment with insulin (0. 1 μmol/L) and dexamethasone (0.3 (μol/L) for 24 h caused optimal insulin resistance of HepG2 cells which lasted for 36 h. Compared with control group, the glucose consumption, intracellular glucose, glycogen, ATP contents and the level of InsR-mRNA in model cells decreased while FFAs concentrations and GR-mRNA increased. However, the tendency of insulin resistant HepG2 cells was obviously attenuated by pioglitazone at the concentration of 0. 2 mmol/L and mifepris tone at 200μmol/L and 20 μol/L while mifepristone at 2 μol/L had no effect on insulin-resistant cells. The findings indicated that mifepristone at 200 μol/L and 20 μol/L improved the insulin resistance via modulating intracellular glucolipid metabolism and the expression of InsR-mRNA and GR-mRNA.