BACKGROUND: Poor mental health status of nursing students can affect their learning and living. OBJECTIVE: To explore mental health status of nursing students at different studying stages.DESIGN: Grouping stratified sampling survey on the basis of nursing students studying in higherschool.SETTING: Nursing Department of the Fourth Military Medical University of Chinese PLA, Laboratory Center of Basic Faulty, and Department of Rehabilitative Physioltherapy of Xijing Hospital.PARTICIPANTS: Totally 515 nursing students at different studying stages of three educational levels (the technical secondary school level, junior college level and undergraduate level) were observed with grouping stratified sampling in December 2004.METHODS: Symptom checklist-90(SCL-90) was used for survey. SCL-90 contained 90 items, being interpreted in the context of nine symptom dimensions, including somatization, obsessive-compulsive, interpersonal sensitivity, depression, anxiety, hostility, phobic anxiety, paranoid ideation, and psychoticism. Each item was scored from grade Ⅰ to grade Ⅴ (symptomless, mild, moderate, little severe, and severe). Scores of total items and 9 symptoms were recorded. The more the scores of total items were, the lower the total level of mental health was; the more the scores of any symptom was, the severer the symptom of mental health was. Data were analyzed with t test. MAIN OUTCOME MEASURES: Scores of total items and 9 symptoms. RESULTS: Totally 515 nursing students were randomly selected in this survey except 5 students because of uncompleted survey, and the response rate was 99.0%. There were 501 qualified questionnaires, which were accounted for 98.2%. Mental health status of nursing students at various stages was different: the score of nursing students in grade 1 and 3 was higher than that in grade 2 in technical secondary school; the score of nursing students was the highest in grade 2 in junior college; and the score of nursing students was the highest in grade 3 at undergraduate level. The order of SCL-90 scores was obsessive-compulsive, interpersonal sensitivity and depression from high to low.CONCLUSION: SCL-90 scores of nursing students are high in grade 1and grade 3 at the technical secondary school level and also in middle grade at junior college level and undergraduate level. The main mental psychological symptoms are obsessive-compulsive, interpersonal sensitivity and depression. Psychological predisposition of nursing students should be cultured according to different psychological characteristics at various learning stages.

BACKGROUND: In vitro culture condition and culture efficiency are different in reported umbilical cord-derived mesenchymal stem cells, and lacked of unified standards. Different derived mesenchymal stem cells have different biological properties. Therefore, it is very necessary to establish a simple and high-performance culture system for umbilical cord-derived mesenchymal stem cells. OBJECTIVE: To observe the growth state of human umbilical cord-derived mesenchymal stem cells in different culture systems in vitro and adenovirus infection efficiency. METHODS: Mesenchymal stem cells were separated from healthy full-termed delivery fetus using collagenase digestion method and purified by adherent culture. These cells were cultured and amplified in DMEM (low glucose), MesenPRO RS~(TM) Medium and STEMPRO~(R) MSC SFM in vitro. The 3-5 passage mesenchymal stem cells were infected by the Ad5-EGFP, Ad5/11-EGFP, Ad5/35-EGFP as multiplicity of infection (MOI)=1, 10, 100. Viral infection and green fluorescence expression were observed at post-infection 24, 56 and 72 hours using inverted fluorescence microscope. RESULTS AND CONCLUSION: The cell morphology in STEMPR~(R) MSC SFM was different from other two culture system and these cells were not easy to adherent after trypsin digestion. Cell doubling time in the MesenPRO RS~(TM) Medium was shorter than other two groups. Mesenchymal stem cells were infected by Ad5/35-EGFP with higher efficiency than other two kinds of adenovirus, but part of cells appeared apoptosis. The infection efficiency of Ad5/11-EGFP was highest. The fluorescence intensity was gradually increased with increased MOI.

Objective To investigate the incidence and influencing factors of depression in empty-nest elderly, so as to provide evidence for reducing depression in empty-nest elderly. Methods During July to August 2016, the elderly over 60 years old in Shaanxi Province, including 504 empty-nest elderly and 194 non-empty-nest, were selected convenience sampling method. The Geriatric Depression Scale (GDS), Social Support Scale, and self-designed questionnaire about the general condition were used. ANOVA was applied to analyze the depression scores; Pearson correlation analysis was used to analyze the relation between social support and depressive symptoms; and Logistic regression analysis had been used to analyze the influencing factors of depression. Results The detection rate on depression of empty-nest elderly was 45.63%, and that of the non-empty-nest elderly was 32.99%, the difference was statistically significant (χ2=10.447,P< 0.05). The scores of GDS of the empty-nest and non-empty-nest elderly were (9.68±5.26) and (8.51±4.69) respectively, and the difference was statistically significant (t=7.390,P< 0.01). Logistic regression analysis showed that living pattern (OR=0.596), degree of education (OR=0.799), age (OR=1.394), place of abode (OR=1.699), happiness degree of later life (OR=1.663) and current life satisfaction (OR=1.474) were correlated with depressive symptom (P<0.05). In addition, Pearson correlation analysis indicated that the social support of the empty-nesters was negatively correlated with depression (r=-0.260,P<0.01). Conclusions The incidence of depression is high in empty-nest elderly, which seriously affects the physical and mental health of them. Targeted measures should be taken to provide more comprehensive social support for empty-nest elderly, improve their life quality and reduce the incidence of depression.

Objective To make volunteer service for the aged of university students as an important complement for the aged care to establish a long-term mechanism of volunteer service for the aged. Methods The teaching model integrated volunteer service for the aged into nursing practice teaching was built by adding practice of community volunteer service for the aged to the courses of community nursing. Results This research structured the organization and management model in volunteer service for the aged nursing practice teaching and preliminarily created an appraisal and motivation mechanism through setting up rules and regulations, evaluation for service effects as well as excitation mechanism. Conclusions We found a teaching model integrated with nursing practice teaching and volunteer service for the aged as well as an approach to attain a long-term and persistent development of volunteer service for the aged so as to provide a practical and theoretical basis basis for a long-term development volunteer service for the aged of university students.

Objective? To understand the heaLth seeking behavior among ruraL residents of Yanchuan County in Yan'an City and to anaLyze its infLuencing factors. Methods? From JuLy 2017 to August 2017, we seLected 552 ruraL permanent residents of Yanchuan County in Yan'an City by convenience sampLing. ALL of them were investigated with the seLf-designed heaLth seeking behavior questionnaire for ruraL residents. The binary Logistic regression was used to anaLyze the infLuencing factors of heaLth seeking behavior among ruraL residents. ResuLts? When ruraL residents were iLL, 224 (40.58%) ruraL residents bought medicines for treatment, 216 (39.13%) knew disease reLated knowLedge and 352 (53.01%) ruraL residents attended ruraL cooperative medicaL care. Binary Logistic regression showed that the infLuencing factors of heaLth seeking behavior of ruraL residents incLuded ages, monthLy income, attending insurance or new ruraL cooperative medicaL care, knowing disease reLated knowLedge, diet ruLes and the proportion of vegetabLes and meats each meaL. ConcLusions? HeaLth conscious of ruraL residents is weak; the heaLth seeking behavior is traditionaL, backward and negative;they Lack knowLedge on heaLth, medicaL treatment, heaLth care and emergency treatment and their medicaL insurance participation is inactive. Government sector shouLd pay attention to heaLth seeking behavior of ruraL residents, strengthen the deveLopment of ruraL doctor team and cLinics in viLLages and towns,improve medicaL care system and ruraL cooperative medicaL care so as to soLve the probLems high cost and difficuLty of getting medicaL service among ruraL residents.

Objective:To explore relationship between serum 25-hydroxy vitamin D ( 25OHD ) level and ketosis-prone in patients with newly-diagnosed type 2 diabetes mellitus(T2DM).Methods:One hundred and thirty-four patients with newly diagnosed T2DM (103 males, 31 females) admitted in The Third Affiliated Hospital, Southern Medical University from January 2017 to January 2019 were enrolled into this study, including 36 patients with ketosis-prone (KPDM group) and 98 patients without ketosis(NKPDM group). Clinical characteristics, including height, weight, and history of hypertensive disease were collected. Serum 25OHD levels, lipid profile, islet function and glycosylated hemoglobin (HbA 1C)levels, ICA, IAA, GAD-Ab, etc., were measured. Results:Among the 134 patients, the patients with vitamin D deficiency, insufficiency, and sufficiency were 71 cases(52.99%), 52 cases(38.81%), and 11 cases(8.20%), respectively. KPDM group had significantly lower serum 25OHD level than NKPDM group[(44.12±9.77) nmol/L vs (55.35±15.31) nmol/L, P<0.01]. The rate of vitamin D deficiency was significantly higher in KPDM group compared to that in NKPDM group [(77.78% vs 43.88%), P<0.01]. The prevalence of KPDM varied significantly in different vitamin D status groups( P<0.01). Logistic regression analysis suggested that low serum 25OHD, younger age, high HbA 1C, and triglyceride were risk factors to ketosis-prone in newly diagnosed T2DM( P<0.01). Conclusion:Vitamin D deficiency is a common problem in newly diagnosed T2DM, especially in KPDM. Low serum 25OHD level seems to be an independent risk factor for ketosis-prone in patients with newly diagnosed T2DM.

Objective:To extract the early result of postoperative echocardiographic evaluation in patients underwent left ventricular assist device (LVAD) implantation, and to assess the efficacy of surgical treatment for end-staged heart failure.Methods:Between June 2019 and May 2023, the patients underwent left ventricular assist device implantation were enrolled in this study. Demographic baseline characteristics and perioperative echocardiographic parameters were collected and analyzed.Results:A total of 28 patients were included in the study. After LVAD implantation, the heart sizes of the patients obviously reduced and the left heart contractibility function improved. The right ventricular contractibility remained stable. The proportion of the patients with moderate to severe mitral regurgitation was significantly reduced, but patients with mild to moderate aortic insufficiency increased. No serious complications such as death, pericardial tamponade and thrombosis events were observed during the follow-up period.Conclusion:LVAD implantation improved the left cardiac function, while the right cardiac function remained stable. However, it should be paid attention that the aortic valve function was impaired after the surgery. Generally, the early results of LVAD implantation for the treatment of end-stage heart failure were satisfactory.