Signal transdcuers and activators of transcription3 (Stat3) is activated by a wide range of cytokines and growth factors following binding to special DNA domain and affects the expression of a variety of genes in modulating the process of cell proliferation, differentiation, and apoptosis. As a point of convergence for many oncogenic tyrosine kinase signaling pathways,it can be activited by sevral accesses. Stat3 is not only in-volved in the function of both apoptosis suppression,proliferation promotion,but also participates the process of angiogenesis,invasion and immune evasion. For repression of this way maybe a new target for break of cancer in-vasion and metastasis,blockers of Stat3 has become the focus of many researches.

To study the Internet addiction disorder,262 college students were investigated by IAD Scale,Internet Usage Inventory,IAS Scale and Shyness Scale.The results showed:(1)Significant difference existed in IAD between freshman and Sophomore,between science and arts student.(2)Significant difference existed in the age of Internet use,on-line place,on-line time and the type of playing games.(3)College students'IAD is closely related to interaction anxiousness and shyness.

Nowadays,antiplatelet and anticoagulant drugs have been applied to a variety of cardiovascular diseases,such as atrial fibrillation and coronary artery disease.Several clinical studies show that there is a higher prevalence of gastrointestinal cancers in patients with cardiovascular disease.In addition,in patients taking antithrombotic drugs,positive fecal occult blood test (FOBT) improves the sensitivity for early detection of colorectal malignancies.This suggests that antithrombotic drugs may facilitate the detection of unrecognized cancers in patients with cardiovascular disease.However,there is no established evidence concerning whether more aggressive gastrointestinal tract screening will reduce the mortality of cancer in cardiology patients.But in an era of multiple antithrombotic drug usage,when patients appear unexplained anemia,gastrointestinal symptoms or positive FOBT,timely gastrointestinal tract screening is helpful to find concomitanted malignancies in these patients.

Objective: To investigate the trends in cancer mortality among residents in Wuyi County, so as to provide insights into the development of the cancer control strategy. Methods: The deaths due to cancer among residents in Wuyi County from 2015 to 2019 were collected from Zhejiang Provincial Information Management System for Surveillance of Chronic Diseases. The crude mortality and standardized mortality of cancer and annual percent change (APC) of were calculated to analyze the trends in cancer mortality and causes of death. Results: A total of 3 424 deaths occurred due to cancer in Wuyi County from 2015 to 2019, and the crude and standardized mortality rates of cancer were 199.65/105 and 185.87/105, respectively. The crude mortality of cancer was significantly higher in men than in women (271.53/105 vs. 125.40/105;χ2=459.374, P<0.001). The standardized mortality of cancer showed a tendency towards a reduction among all study subjects, men and residents at ages of 45 to 59 years (APC=-3.87%, -4.44%,-11.46%, all P<0.05), and the crude mortality of cancer appeared a tendency towards a rise with age in all study subjects, men and women (χ2=7 063.842, 2 155.609, 5 492.128, all P<0.001). Lung cancer, liver cancer, gastric cancer, bowel cancer and esophageal cancer were the five leading causes of cancer-related mortality, which accounted for 71.41% of all cancer mortality. The crude mortality of central nervous system cancer (2.07/105) and leukemia (1.66/105) was high among residents at ages of 0 to 14 years, and the crude mortality of liver cancer (3.02/105) and lung cancer (2.01/105) was high among residents at ages of 15 to 44 years, while the crude mortality of lung cancer (51.57/105) and liver cancer (36.22/105)was high among residents at ages of 45 to 59 years, and the crude mortality of lung cancer (305.48/105) and gastric cancer (114.65/105) was high among residents at ages of 60 years and older. Conclusions The standardized mortality of cancer appeared a tendency towards a reduction in Wuyi County from 2015 to 2019, and men and the elderly were high-risk populations for cancer. Screening, early diagnosis and treatment of lung cancer, gastrointestinal cancer and cervical cancer should be given a high priority.

Objective To evaluate the effects of sevoflurane preconditioning on lung injury induced by renal ischemia-reperfusion (I/R) in rats.Methods Forty adult male Wistar rats,aged 3 months,weighing 200-250 g,were randomly divided into 5 groups (n =8 each):sham operation group (group S); renal I/R group (group I/R); 1.1％ sevoflurane preconditioning group (group SP1); 2.2％ sevoflurane preconditioning group (group SP2); and 3.3％ sevoflurane preconditioning group (group SP3).Renal I/R was induced by clamping bilateral renal pedicles for 45 min followed by 6 h reperfusion.In SP1-SP3 groups,1.1％,2.2％ and 3.3％ sevoflurane were inhaled for 1 h,respectively,before ischemia,followed by 10 min washout.The rats were sacrificed by exsanguination at 6 h of reperfusion,and lungs were removed for determination of wet/dry lung weight (W/D) ratio,myeloperoxidase (MPO) activity and expression of angiotensin converting enzyme (ACE) mRNA,and for microscope examination of pathologic changes which were scored.Results Compared with group S,the pathological score,W/D ratio,MPO activity and expression of ACE mRNA were significantly increased in I/R and SP1-SP3 groups (P ＜ 0.05).Compared with group I/R,the pathological score,W/D ratio,MPO activity and expression of ACE mRNA were significantly decreased in SP1-SP3 groups (P ＜ 0.05).Compared with SP1 and SP3 groups,the pathological score,W/D ratio,MPO activity and expression of ACE mRNA were significantly decreased in group SP2 (P ＜ 0.05).Compared with group SP1,the W/D ratio was significantly decreased (P ＜0.05),and no significant changes were found in the other parameters in group SP3 (P ＞ 0.05).Conclusion Sevoflurane preconditioning can attenuate lung injury induced by renal I/R and inhibition of ACE synthesis is involved in the mechanism.

Cerebral palsy (CP) is a essential disorder result in disability of children. Various treatments, especially rehabilitation, have been used and made some success for CP. This paper reviewed a number of literatures published last decade. The level of evidence and recommendation grade of various treatments was evaluated according to Cochrane.

Along with the transformation of the pattern of the medicine and the development of dental medicine,more and more patients accept orthodontic treatment.The patients have higher expectations of the treatment.As a result,the abilities to make clinical communication for orthodontic postgraduates should be improved.The article discusses how to improve the orthodontic postgraduates' abilities in the hope that it will be of some help to them.

Objective To investigate the expression and clinical significance of UL16 binding protein 3 (ULBP3) in human esophageal squamous cell carcinoma (ESCC) and its correlation with nature killer (NK) cells.Methods The relative expression of ULBP3 in the ESCC tissues and corresponding carcinoma adjacent tissues of 40 patients was detected by realtime-poly merase chain reaction (PCR),immunohistochemical staining and Western blot methods.The percentage of NK cells in peripheral blood of same patients was examined by flow cytometry.The correlation between ULBP3 and the percentage of NK cells was analyzed with Pearson method.Results The expression of ULBP3 at mRNA level in the tumor tissues ([4.96 ±-6.11]×10-3) was significantly higher than that of corresponding carcinoma adjacent tissues ([1.64 ± 2.96]× 10-3,t =3.656,P＜ 0.01).The immunohistochemical staining results indicated that the positive rate of ULBP3 in the tumor tissues was 60％ (24/40),however that of corresponding carcinoma adjacent tissues was only 32.5％(13/40,t=3.921,P＜0.01).The Western blot results indicated that the expression of ULBP3 at protein level in the tumor tissues was significantly higher than that in the corresponding carcinoma adjacent tissues.The relative expresssion ULBP3 at mRNA level in carcinoma tissues of ESCC patients with lymph node metastasis and at TNM stage Ⅲ was higher than that of ESCC patients without metastasis and at TNM stage Ⅰand Ⅱ (t=4.839,4.192,P＜0.05).There was no significant correlation between the expression and ages,gender,location of tumors and the differentiation degree of tumor (P＞0.05).At early and mid stage of the tumor,the expression of ULBP3 at mRNA level was positively correlated with the percentage of NK cells in peripheral blood (r=0.5233,P＜0.05),however there was no correlation at advanced stage.Conclusion ULBP3 was highly expressed in ESCC and may be involved in the immune regulation of NK cells.

Objective To analyze the correlation between the bleeding control time,treatment and prognosis in acute gastroesophageal variceal hemorrhage and to explore the predictive factors in treatment failure.Methods The clinical data of 309 patients with acute gsstroesophageal variceal hemorrhage was collected.The bleeding control was retrospectively analyzed within and at two hours after treatment,over two hours but less than six hours,over six hours but less than five days,at and after five days.The correlation between bleeding control time and mortality rate was analyzed.The differences in success rate of bleeding control and mortality rate between medication treatment alone and medication combined with endoscopic treatment were compared.Logistic regression mode was used to analyze the predictive factors of treatment failure.Results Of the 309 acute gastroesophageal variceal hemorrhage hospitalized patients,the failure of bleeding control within and at two hours,over two hours but less than six hours and over six hours but less than five days were 79 cases,25 cases and 26 cases respectively and there were one,four and 16 cases who died respectively.The success rate of bleeding control by medication treatment alone was 57.93％ (179/309) and the mortality was 10.36％ (32/309). The success rate of bleeding control by medication combined with treatment under endoscope was 82.35％ (28/34) and the mortality rate was 0.The results of Logistic regression mode analysis indicated that the admission systolic blood pressure (≤90 mm Hg,1 mm Hg=0.133 kPa),increase of total bilirubin,increase of Child-Pugh grade and ascites were the predictive factors of treatment failure.Conclusions The time of bleeding control failure mostly was within two hours.With time extended,the mortality increased. The success rate of bleeding control by medication combined with endoscopic treatment was higher and the mortality was lower.The admission systolic blood pressure lower than 90 mm Hg,increase of total bilirubin,increase of Child-Pugh grade and ascites may be the predictive factors of treatment failure.

Objective To explore the potential significance of long noncoding RNA (lncRNA) expressed in endoplasmic reticulum stress response also known as unfolded protein response ( UPR) .Methods Mouse embryonic fibroblasts ( MEFs) were isolated from 13 day mouse embryo , cultured and treated with tunicamycin for 16 hours to induce UPR .The untreated MEFs were used as negative control .The lncRNA expression profile was examined by a customized lncRNA array. The results of array assay were validated by real-time PCR and further analyzed by bioinformatics. Results There were 411 lncRNAs whose expression was signficantly up-regulated in MEFs treated with tunicamycin as compared with the controls ,while 790 lncRNAs were significantly down-regulated. A number of significantly alter lncRNAs were validated by real-time PCR. A further bioinformatics analysis of lncRNA profile suggested that many cold be in-volved in multiple cellular pathways. Conclusion Our lncRNA expression profile and bioinformatics studies srongly suggest that many lncRNAs can be regulated in the cells under UPR and could also in turn regulate UPR and other cellular processes.