Objective To investigate the effects of arsenic trioxide (ATO) on the expression of autoan-tibody and interleukin (IL)-10 IL-12 in MRL/lpr mice. Methods MRL/lpr mice wereseparated into 3 different groups. The 3 groups received arsenic trioxide (ATO, 0.4 mg·kg~(-1)·d~(-1)), cyclophosphamide (CTX,50 mg/kg) and sodium chloride (NS, volume weight-determined) abdominal injection respee-tively. The treatment stopped 2 months later. Afterwards, the rates of CD3~+(T) cells, CD3~+CD4~+(Th) cells and the CD3~+CD4~+cells which produced IL-10 and IL-12 were detected using single-cell measurement of intr-acellular eytokines by flow cytometry after polyclonal stimulation with PMA and ionomycin for 4 hours in 5% CO_(2.)Serum levels of IL 10 and IL-12 were assessed using the Mouse cytokines ELISA Kit. One-way ANOVA LSD test and paires t test were used for statistical analysis.Results ①The level of anti-dsDNA antibody after treatment was 0.92±0.06, while it was 1.14±0.58 before treatment. So the ds-DNA antibody level was significantly decreased in ATO group (P<0.01), while it was dramatically increased in the NS groups (P<0.05) after the treatment;②ATO group had significantly less CD3~+ cells and CD3~+CD4~+ cells[(44±4)% and (20±4)%]compared withNS group [(59±5)%and(30±3)%](P<0.01).③The serum level of IL-12 in the ATO group was (84±12) pg/ml,while it was (103±13)pg/ml in the NS group (P=0.018).④The intracellular levels of IL-10 and IL-12 produced by CD3~+CD4~+ (Th) cells in the ATO group were ( 1.5±0.4)% and (2.43±0.42)%, which was significantly lower than those in the NS group respectively (2.5±0.5)% and (3.24±0.40)%(P<0.01). Conclusion Arsenic trioxide can reduce the production of anti-dsDNA antibody,inhibit the activation and proliferation of both T cells and Th subsets in the MRLApr mice, and hence decrease the serum levels of IL-12 and the levels of IL-10, IL-12 produced by Th cells.

Objective To investigate the health-related quality of life(HRQOL)of the recovered patients with depression and its influential factors.Methods From March 2008 to April 2009,150 depressive patients andergoing the following up treatment were recruited,and finally 124 patients rocovered,and quality of life for patients recovered were compared to published norms for the general Chinese people.The general demography material seale.Hamilton Depression Scale(HAMD),Hamilton Anxiety Scale(HAMA),personality traits(Eysenck personality Inventory-EQP)the short form 36 item(SF-36)questionnaire and social support scale(SSS)were applied to an subjects.The SPSS 13.0 software for data processing,the group t test and the multiple linear regression analysis was adopted.Results ①The average score of seven domains of quality of life(RP,BP,GH,VT,SF,RE,MH )for the recovered patients were better than that of the pre-treatment patients(P<0.01),except the PF(89.23±14.09,87.23±16.81,t=1.044,P>0.05).But the average score of seven domains of quality of life for the re-covered patients were worse than that of the healthy people(P<0.01),except the PF(89.23±14.09,89.01±15.73,t=1.266,P>0.05).②The quality of life 0f the recovered patients remarkably related with the anxiety level, neuroticism, extraversion, recurrent depression,economic condition and social support(P<0. 01) . Conclusion The recovered depressive patient s health-related quality of life were improved significantly afer treatment, what were influenced by neuroticism,extraversion,anxiety as a trait,recurrent depression,economic condition and social support.Above mentioned factors should be taken into account when rehabilitation.

Objective To explore the preventive effect of the supplemental dietary boron on bone damage of rats with excess fluoride (EF) ingestion. Methods Twenty-four Sprague-Dawley rats aged 4-5 weeks old were divided into the control (C group, treated with distilled water and the elementary dietary), the excessive fluoride dose group (EF group, treated with distilled water with 100mg/L F-from 221.0g NaF per liter and the elementary dietary) and the boron prevention group (P group, treated with 100mg/L F-distilled water and the supplemental boron dietary). Three months after the experiment, fluorine, boron contents and AKP activities in serum, total RNA and fluorine contents in bone, vertical and transverse diameter and dry weight of tibias of rats were assayed, and bone biomechanics of femur and bone mineral density (BMD) were determined. Results Compared with those in the C group, fluorine contents in serum and bone, AKP activities in serum of rats in the EF group significantly increased; total RNA in bone and BMD of the bone of whole body and tibia decreased, vertical and transverse diameter of tibias was shortened, dry weight was decreased; peak load of femur increased but maximum deformation decreased. Compared with those in EF group, fluorine contents in serum and bone, AKP activities in serum of rats in the P group obviously decreased; total RNA in bone and BMD of whole body, lumbar vertebrae and tibias notably increased, vertical and transverse diameters and dry weight of tibia were enhanced, peak load of femur was obviously raised. Boron contents in serums of rats in three groups had no significant differences. Conclusion Bone damage obviously occurred in rats with EF intake, and the supplemental dietary boron had a preventive effect on these changes.

Objective:To investigate the variation characteristics and influencing factors of HIV/AIDS subtypes in Wuxi city of Jiangsu Province from 2014 to 2016.Methods:HIV/AIDS population in Wuxi city in 2014 was selected as the research object, and the HIV molecular epidemiology and follow-up study were carried out. Collect epidemiological information, extract DNA from blood samples, amplify pol gene fragment by nest-PCR and sequence, use ChromasPro 1.6 software and MEGA 7.0 software to construct the HIV-1 sequence database, and use FastTree2.1.10 software to construct the phylogenetic tree to confirm the subtype; in 2016, the same population was followed up, and the HIV subtype variation was analyzed, and the influencing factors of subtype variation were explored by multivariate logistic regression. Results:A total of 612 HIV/AIDS cases in 2014 and 2016 were collected. The age of the subjects was mainly 30 years old or above (85.46%, 523/612), and the proportion of people over 50 years old was higher (228/612, 37.25%). The main route of transmission was homosexuality, accounting for 49.67%. A total of 1224 samples were detected and CRF01 _ AE、CRF07_ BC、B、CRF08_ BC、CRF67_ 01B、CRF55_ 01B、CRF68_ 01B, 7 subtypes of HIV-1 and 5 unique recombinant types (URFs) was detected. CRF01_ AE and CRF07_ BC was still the main genotype in Wuxi, Jiangsu Province, accounting for 66.75%. There were 29 cases (3.56%) of URFs recombinant strains. During 2014-2016, the variation rate of subtypes was 14.63%, and the most common variation was CRF01_ AE changes to CRF07_ BC(13.95%). Marital status (OR=0.363, 95% CI: 0.137-0.964) and baseline CD4 level (OR=0.414, 95% CI: 0.192-0.891) were associated with subtype variation.Conclusions:The HIV-1 subtypes of HIV/AIDS patients in Wuxi city are diverse and complex, the proportion of recombinant subtypes is rising, the URFs that are difficult to determine the genotype increase significantly, and the variation rate of HIV-1 subtypes among HIV/AIDS infected people is high. It is necessary to strengthen the monitoring of HIV-1 subtypes.

Objective? To expLore the status quo of cLinicaL traditionaL Chinese medicine (TCM) nursing training between 2007 and 2017, and to provide a reference and guidance for further standardizing TCM nursing training and conducting reLevant research. Methods? The articLes reLated to TCM nursing training which were pubLished between 2007 and 2017 were retrieved from CNKI, CBM and VIP databases and anaLyzed using bibLiometric methods. ResuLts? TotaLLy 109 vaLid articLes were found, and the number of articLes pubLished over the past ten years tended to increase; the articLes were pubLished on 54 journaLs in which those incLuded in Chinese core journaLs of Peking University accounted for 1.8%; most of the first authors were from Guangdong, Zhejiang and Jiangsu provinces; the research mainLy focused on the appLication of various training methods in TCM nursing training and the discussion of TCM nursing skiLLs; the degree of cooperative authorship was 2.7 persons, and the funding rate was 32.1%; junior and intermediate nurses accounted for 61.9% of the training subjects, and there were a few senior and speciaLized nurses, with community nurses accounting for onLy 3.7%;and the top 5 evaLuation indicators were theoreticaL performance, operationaL performance, nurse's satisfaction, patient's satisfaction and quaLity of nursing. ConcLusions? TCM nursing has become increasingLy important, but the quaLity of reLevant research is Low. There is no in-depth and extensive research in this regard, nor standardized study design and training modeL. Few studies have used mobiLe information pLatforms, and the evaLuation indicators are not objective or uniform.

Vertebrobasilar dolichoectasia(VBD ) can increase the risk of stroke. Significant expansion, elongation and tortuosity of the vertebrobasilar arteries are the main morphology manifestations of VBD. However, there is no consensus on the quantitative imaging assessment of VBD. Many studies showed that some quantitative parameters, such as basilar artery length and bending length, basilar angulation, vertebral tortuosity index can be used for the quantitative imaging assessment of VBD.

Objective? To explore the effects of aromatherapy on ameliorating nausea and emesis in orthopedic patients after surgery under general anesthesia. Methods? Totally 180 patients treated in the Department of Orthopedic Trauma, Suzhou Hospital of Traditional Chinese Medicine from November 2017 to July 2018 by convenient sampling, and the orthopedic patients receiving general anesthesia were divided into the treatment group (n=90) and the control group (n=90) according to the random serial generated by SPSS. Patients in the control group sniffed saline and received conventional nursing care, while patients in the treatment group received aromatherapy with essential oil and conventional nursing care. The incidence and severity of postoperative nausea and emesis was compared between the two groups. Results? The incidence rate of nausea and emesis in the treatment group within 24 hours post operation was 13.33%, lower than that in the control group, which was 51.11%, and there was statistically significant difference (χ2=29.406,P＜0.05). The number of patients showing nausea and emesis with different degrees in the treatment group within 24 hours post operation was Class 0 of 78 cases,8 cases of ClassⅠ, 4 of ClassⅡ and 0 of ClassⅢ,respectively. The number of patients showing nausea and emesis with different degrees in the control group within 24 hours post operation was Class 0 of 44 cases, 15 cases of Class Ⅰ, 27 of Class Ⅱ and 4 of Class Ⅲ,respectively. The severity of nausea and emesis in the treatment group was lower than that in the control group, and there was statistically significant difference (Z=-5.656,P＜ 0.05). Conclusions? Aromatherapy can effectively reduce the incidence rate and severity of nausea and emesis in orthopedic patients after surgery under general anesthesia.

OBJECTIVETo investigate the effect of ginsenoside Rg1 on the expressions of phosphory protein Tau (P-Tau), N-methyl-D-aspartate receptor subunit 1 (NR1) and N-methyl-D-aspartate receptor subunit 2B(NR2B) in rat brain slice model of Alzheimer's disease.

METHODBrains of 5-week-old Wistar rats were cut into slices which were 400 microm thick. These brain slices were randomly divided into normal control group, untreated group, low-dose ginsenoside Rg1 group, medium-dose ginsenoside Rg1 group and high-dose ginsenoside Rg1 group, with 10 slices in each group. All brain slices were cultured with artificial cerebrospinal fluid (ACSF) that was aerated via polyethylene tubing attached to a source of 95% O2, 5% CO2 at (32.0 +/- 0.5) degrees C. And brain slices in the ginsenoside R1 groups were administrated with the ginsenoside Rg1 (60, 120 and 240 micromol x L(-1) respectively) in ACSF for 2 h firstly. Then okadaic acid (OA) was administrated into ACSF of untreated group and ginsenoside Rg1 groups separately for 3 h to induce Tau phosphorylation to prepare AD models. The concentration of OA in each group was 1 micromol x L(-1). And there was no any intervention for the brain slices in the normal control group. The expressions of P-Tau, NR1 and NR2B in brain slices in each group were determined by immunohistochemical method, and the results were analyzed by image acquisition and analysis system.

RESULTCompared with the normal control group, the expression of P-Tau was significantly increased (P < 0.05 or P < 0.01) and the expressions of NR1 and NR2B were decreased (P < 0.01) in untreated group. Compared with the untreated group, the expression of P-Tau was significantly decreased (P < 0.01 or P < 0.05) and the expressions of NR1 and NR2B were increased (P < 0.01 or P < 0.05) in ginsenoside Rg1 groups, especially in high-dose ginsenoside Rg1 group.

CONCLUSIONGinsenoside Rg1 can play the role of anti-dementia by inhibiting the expression of P-Tau so as to slow the formation of neurofibrillary tangles and increasing the expression of NR1 and NR2B so as to improve learning and memory abilities in rat brain slice model of Alzheimer's disease.

Alzheimer Disease ; metabolism ; pathology ; Animals ; Brain ; drug effects ; metabolism ; pathology ; Disease Models, Animal ; Gene Expression Regulation ; drug effects ; Ginsenosides ; pharmacology ; Hippocampus ; drug effects ; metabolism ; pathology ; In Vitro Techniques ; Male ; Phosphoproteins ; metabolism ; Rats ; Rats, Wistar ; Receptors, N-Methyl-D-Aspartate ; metabolism ; tau Proteins ; metabolism

OBJECTIVETo observe the effect of ginsenoside Rg1 on the expressions of phosphory protein Tau (P-Tau) and caspase-3 in brain slices from AD model rats.

METHODThe brains of 5-week-old Wista rats were cut into slices which were 400 microm thick. These brain slices were divided into five groups: normal contral group, untreated group, low-dose, medium-dose and high-dose ginsenoside Rg1 groups (60, 120, 240 micromol x L(-1)). And there were 10 slices in each group. These brain slices were cultured with artificial cerebrospinal fluid. After the brain slices in ginsenoside Rg1 groups were administration with ginsenoside Rg1 for 2 h preventively, brain slices in untreated group and ginsenoside Rg1 groups were administrated with okadaic acid (OA) for 3 h to induce hyperphosphorylation of Tau protein to prepare AD models. And the effects of ginsenoside Rg1 on the expressions of P-Tau and caspase-3 in brain slices from AD model rats in each group were observed with immunohistochemistry and image analysis technology.

RESULTThe levels of the expressions of P-Tau and caspase-3 in the untreated group were significantly higher than those in the normal control group (P < 0.01). Compared with untreated group, the levels of the expressions of P-Tau and caspase-3 in ginsenoside Rg1 groups were significantly low (P < 0.01 or P < 0.05).

CONCLUSIONGinsenoside Rg1 could inhibit the expression of P-Tau to slow the formation of neurofibrillary tangles and could inhibit the expression of caspase-3 to inhibit neuronal apoptosis to protect the nerve cells, so as to play the role of anti-dementia.

Alzheimer Disease ; drug therapy ; metabolism ; Animals ; Brain ; drug effects ; metabolism ; Caspase 3 ; metabolism ; Disease Models, Animal ; Ginsenosides ; therapeutic use ; Immunohistochemistry ; Male ; Rats ; Rats, Wistar ; tau Proteins ; metabolism