OBJECTIVE To detect bacteria number of the mattress in the common ward in order to reduce the mattress transmitted hospital infection. METHODS Secretion was collected for bacteria test from mattresses of the five common wards at the d0,dd1-6,dd7-13,dd14-20,and d21 and more after admission of patients. RESULTS The longer the patients stayed,the heavier the pollution of mattress was,its bacterial amount was much higher than the criterion made by Ministry of Health(10 CFU/cm~2). CONCLUSIONS Long-term hospitalization is the main factor that makes mattress bacterial contaminant in the common ward,and it should be cleaning and sterilizing regularly.

OBJECTIVE: To explore the positive expressing rates of oncogene Survivin and tumor-suppressor gene PTEN in transplanted laryngo-carcinoma of nude mice treated by Gold Throat Tablets (GTT) which can improve circulation and remove haemostasis in TCM theory. METHOD: The 32 nude mice seeded with cultured laryngeal carcinoma cells subcutaneously at the back were randomly divided into high, middle, low (according to 6 : 3: 1 proportion of GTT dosing given by intragastric administration) dose groups and blank control groups. The changes on weight and size of tumors originated from these cells were observed for 28 days, and the density of tumors and expression of Survivin and PTEN were examined with tumor sections by immunohistochemical assay after separating tumors from back of nude mice. RESULT: The weight and size of subcutaneous laryngo-carcinoma on backs of high dose group nude mice were both the smallest among all the experimental groups with the average density of tumors as 1.202 g/cm3. The positive expressing rates of Survivin and PTEN both revealed the following tendency that high dose group < middle dose group < low dose group < blank control group. CONCLUSION Six times of regular doses of GTT can prevent overgrowth of laryngo-carcinoma transplanted on nude mice and decrease the excessive expression of oncogene Survivin in the tumor. PTEN, expressing lower than Survivin in all groups, shows a subordinative relationship with it, maybe due to a competition mechanism.
Animals ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Cell Line, Tumor ; Drugs, Chinese Herbal ; pharmacology ; Female ; Humans ; Inhibitor of Apoptosis Proteins ; metabolism ; Laryngeal Neoplasms ; metabolism ; pathology ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Repressor Proteins ; metabolism ; Survivin

OBJECTIVE: Through exploring the differentiation on positive expressing rates between oncogene survivin and tumor-suppressor gene PTEN (phosphatase and tensin homolog deleted on chromosome ten) on vocal cord polyps (VCP), adult type laryngeal papilloma (ALP), and laryngeal squamous cell carcinoma (LSCC), to reveal the mechanism in cancellation of human laryngeal squamous cells, from benign proliferative lesion, benign tumor to malignant tumor in larynx. METHOD: After picking out 18 cases of VCP, 10 cases of ALP, and 18 cases of LSCC for immunohistochemical process of Survivin and PTEN with two continuous section preparations, the differentiations of positive expression rates of Survivin and PTEN in the same human laryngeal squamous cells areas among three diseases were compared. RESULT: The positive expressing rates of survivin and PTEN in VCP were obviously more lower than in ALP and LSCC (P < 0.05), which showed no obvious difference between each other(P > 0.05). The positive expressing rates of survivin were always higher than PTEN in VCP, ALP and LSCC evidently (P < 0.05). CONCLUSION PTEN, expressing for competition purpose, shows a subordinative relationship with Survivin. Although they have opposite functions in determine whether the cancellation of laryngeal squamous cells take place or not, Survivin is always playing the leading role and making predominant impact on development of benign proliferative lesion, benign and malignant tumor in larynx.
Adult ; Aged ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Humans ; Inhibitor of Apoptosis Proteins ; metabolism ; Laryngeal Neoplasms ; metabolism ; pathology ; Middle Aged ; PTEN Phosphohydrolase ; metabolism ; Polyps ; metabolism ; pathology ; Survivin ; Vocal Cords ; pathology

OBJECTIVE To investigate the clinical characteristics of glucocorticoid allergy， and provide treatment and prevention strategies for patients with concurrent bronchial asthma. METHODS A retrospective analysis was conducted on the clinical data of 250 patients with suspected glucocorticoid allergy admitted to the First Affiliated Hospital of Army Medical University （hereinafter referred to as “our hospital”）from May 1st， 2002， to April 30th， 2022； and a typical case analysis was carried out. RESULTS Among 250 patients with suspected glucocorticoid allergy， 140 were female patients （56.00%） and 110 were male patients （44.00%）. The majority of admissions were to the internal medicine department （118 cases， 47.20%）. One hundred and seventy patients （68.00%） were allergic to dexamethasone， and 37 patients （14.80%） were allergic to prednisone. Two hundred and thirty-eight patients （95.20%） were allergic to one type of glucocorticoid， and 12 patients （4.80%） were allergic to two types of glucocorticoid. Ten patients had clear records of glucocorticoid administration routes， in which 6 were intravenous and 4 were oral. Among the 250 patients， only 32 cases had clear records of clinical manifestations of glucocorticoid allergy， the most common clinical manifestations were rash （12 cases） and skin itching （8 cases）， with 6 patients experiencing both rash and skin itching. The treatment for glucocorticoid allergy mainly involved discontinuing the medication and providing symptomatic treatment. For patients with concurrent bronchial asthma， it was generally necessary to switch to other glucocorticoids in their subsequent treatment to control the condition. The incidence of suspected glucocorticoid allergy among inpatients in our hospital during the same period was 0.019%. The incidence of suspected glucocorticoid allergy with concurrent bronchial asthma was 0.201%. Additionally， a case analysis of bronchial asthma combined with chronic obstructive pulmonary disease in our hospital resulted in glucocorticoid allergy revealed that， based on the patient’s medical history and drug challenge test results， the patient was diagnosed with glucocorticoid （Methylprednisolone tablets and Dexamethasone acetate tablets） allergy， and antihistamine treatment was effective. CONCLUSIONS Glucocorticoid allergy is clinically rare， and its clinical manifestations are predominantly mild reactions such as rashes and skin itching. For patients with concurrent bronchial asthma who experience glucocorticoid allergy， treatment options include switching to other glucocorticoids， altering the route of glucocorticoid administration， symptomatic treatment， and the use of immunosuppressive agents as adjunctive or alternative therapy.