AIM: To investigate the effects of protein C activator (PCA) from Agkistrondon acutus venom ( AAV) on the tension of thoracic aorta rings isolated from the rats with sepsis.METHODS:The model of sepsis was es-tablished by intraperitoneal injection of lipopolysaccharide ( LPS) .SD rats were randomly divided to 6 groups ( n=6 ):sham group, LPS group, PCA intervention group (LPS+PCA, PCA at doses of 0.1 mg/kg, 0.3 mg/kg and 0.6 mg/kg) and LPS+polymyxin B (at dose of 0.2 mg/kg) group.Using perfusion experiment in vitro, the tension of the aortic rings was measured by biological signal analytical system.RESULTS:The values of MABP, HR, LVDP and ±dp/dtmax were significantly lower in LPS group than those in sham group and LPS+PCA groups.Compared with sham group, the relaxa-tion response to acetylcholine ( ACh) and the contractile response of aorta rings induced by phenylephrine ( Phe) were sig-nificantly decreased in LPS group, which were increased significantly in PCA intervention group ( especially at dose of 0.6 mg/kg) compared with LPS group.The dose-response curve of aorta contraction with denuded endothelium induced by Phe shifted down significantly in LPS group compared with sham group, and no significant difference between LPS group and PCA intervention group was observed.Also no statistical difference was found in non-endothelium dependent relaxation of aortic rings induced by sodium nitroprusside among the groups.Pretreatment of N-nitro-L-arginine methl ester and methyl-ene blue increased the contraction amplitude of aortic rings induced by Phe.CONCLUSION:PCA from AAV effectively reverses the hypoergia of the vessels in rats with sepsis through protecting vascular endothelium, the mechanism of which may be mediated by inhibiting NO-GC-cGMP signal transduction pathway.

Objective: To investigate college students’ energy drink(ED) consumption and associated factors, and to provide a reference for maintaining college students’ mental and physical health. Methods: Stratified cluster sampling was used to select 2 264 college students to complete questionnaires for the condition of energy drinking and associated influencing factors. Results: Within a month, 51.0% college students had drunk ED, 46.8% students’ drinking frequency was ≤9 times, 4.2% students’ drinking frequency was ≥10 times; there were significant differences in different gender, major, grade, BMI, smoking, drinking, gambling and weight controlling behaviors students’ ED consumption frequency(χ2 were 168.91, 149.08, 92.92, 25.20, 83.42, 85.77, 11.50, 20.50 respectively, both P<0.01), the males’(65.6%) was higher than the females’ (38.2%), the sports majors’(74.1%) was higher than the arts’, sciences’ and painting majors’(45.6%, 44.6%, 42.2%), the freshmen and sophomores’(58.9%, 58.2%) were higher than the juniors’(37.4%), the normal and overweight-obesity BMIs’(53.5%, 54.4%) were higher than the too low BMIs’(40.5%), the smokers’(68.4%) was higher than the non-smokers’(45.7%), the drinkers’(63.5%) was higher than the non-drinkers’(43.4%), the gamblers’(62.1%) was higher than the non-gamblers’(49.8%), the dieters’(53.9%) was higher than the non-dieters’(43.2%); the gender, major, grade, smoking, drinking and weight controlling behaviors were associated factors on college students’ energy drink consumption, males‘consumption was more than female’s(OR=3.13), freshmen and sophomores’ consumption were more than juniors’ (OR=2.40, 2.23), the students’ consumption of sports majors was more than that of science and painting major(OR=0.28, 0.48), students with smoking, drinking and losing weight consumed more ED than students without these behaviors(OR=0.77, 0.80, 0.79). Conclusion More than half college students had drunk ED; society,school and home should pay attention to this phenomenon, and take measures to reduce ED consumption frequency, to maintain students’ physical and mental health.