Objective To investigate degenerative changes of auditory functions and age -related expression of phosphorylated extracellular signal -regulated protein kinases1/2(p-ERK1/2) in the cochlea of the senescence accelerated mouse .Methods The 3 ,5 ,7 months old mice were selected for the study and each group had 6 mice . The 8 kHz tone burst auditory thresholds and age -related expression of p -ERK1/2 in the cochlea were studied in the senescence accelerated mouse/prone 8(SAMP8) at 3 ,5 ,7 months .The expression of p -ERK1/2 was analyzed by the optical density of immunohistochemical staining .Results For the auditory function evaluation :The SAMP 8 developed a progressive hearing loss at 8 kHz which showed an age -related significant increase (P< 0 .05) .The ABR thresholds in 3 ,5 ,7 months old groups in the left ear were 31 .817 ± 1 .228 ,54 .329 ± 1 .459 ,58 .330 ± 1 .252 dB SPL ,respectively .In the right ears ,the ABR threshold were 32 .474 ± 1 .041 ,53 .485 ± 1 .385 ,57 .842 ± 1 .173 dB SPL ,respectively .p-ERK1/2 protein expressed in the cochlea of the SAMP8 throughout the development sta‐ges ,which developed an age-related significant decrease (P<0 .05) .The average optical density of p -ERK1/2 in the spiral ganglion cells in the 3 ,5 ,7 months old mice were 0 .699 7 ± 0 .018 8 ,0 .621 5 ± 0 .014 7 ,0 .575 3 ± 0 .015 5 ,respectively .In the hair cells ,they w ere 0 .651 9 ± 0 .025 2 ,0 .591 2 ± 0 .010 2 ,0 .559 3 ± 0 .006 7 respec‐tively .Conclusion The expression level of p -ERK1/2 protein decreases when the SAMP 8 develops a progressive hearing loss .This indicates that p-ERK1/2 protein probably has relationship with maintaining functional status of the cochlea and the auditory formation .

Objective To explore hearing loss , the expression of age-related changes of nerve growth factor receptor TrkA (NGFR TrkA ) in the cochlea of the senescence-accelerated mouse. Methods The senescence accelerated mouse/prone 8(SAMP8) at 3, 5, 7 months were chosen as analyzing subjects. The auditory thresholds was monitored by auditory brainstem respons (ABR). The expression of NGFR TrkA protein was analyzed by the optical density of immunohistochemical staining. Results The SAMP8 developed a progressive auditory threshold which showed an age-related significant increase (P < 0.05). There were NGFR TrkA protein expressed in the cochlea of the SAMP8 throughout the development, which developed an age-related significant descend (P<0.05). Conclusion The expression level of NGFR TrkA protein decreases when the SAMP 8 develops a progressive hearing loss which indicates that NGFR TrkA protein probably has relationship with maintaining functional status of the cochlea.

Objective To investigate the biological features of the mouse bone marrow stromal stem cells (BMSCs) transfected by humanβnerve growth factor(β-NGF) .Methods BMSCs of GFP transgenic mouse were isolated and cultured .Theβ-NGF re-combinant plasmid vectors were transferred into the cultured BMSCs by LipofectamineTM 2000 .The expression of β-NGF was detec-ted with ELISA .Results The β-NGF recombinant plasmid vectors were successfully transferred into BMSCs of GFP transgenic mouse ,the expression of β-NGF in transfected cells appeared .In addition ,the expression ofβ-NGF could effectively protect the BM-SCs which were injured in transfection process .Conclusion BMSCs of GFP transgenic mouse after transfecton with human β-NGF have the proliferation and differentiation capacity ,and possess the expression ability of β-NGF protein .

OBJECTIVE: To study the effects of epigallocatechin-3-gallate (EGCG) on proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cell line and analyze the expression of Bcl-2, Bax and Caspase-3 in the cell line which treated with EGCG. METHOD: MTT assay and flow cytometry were used to analyze cell proliferation and cell cycle. Hoechst33258 fluorescence staining was adopted to study cell apoptosis. RT-PCR was used to detect the expression of Bcl-2, Bax, Caspase-3. RESULT: EGCG could significantly inhibit proliferation of CNE-2 cell line and induce its apoptosis with dose-independent relationship. EGCG could suppress the expression of Bcl-2 and induce expression of Bax, Caspase-3. CONCLUSION EGCG in vitro has efficacy of anti-nasopharyngeal carcinoma cells, which may be through regulating the expression of cell proliferation and apoptosis genes involved.
Apoptosis ; drug effects ; Carcinoma ; Caspase 3 ; metabolism ; Catechin ; analogs & derivatives ; pharmacology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Humans ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; metabolism ; pathology ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; bcl-2-Associated X Protein ; metabolism

Objective To observe the expressions and change of calcitonin receptor-like receptor(CRLR) and calcitonin generelated peptide receptor component protein(CGRP-RCP)in venous blood of New Zealand rabbit with allergic rhinitis and to analyze its effective mechanism.Methods Twenty-four New Zealand rabbits were divided into the normal control group(A) and allergic rhinitis group(B).Ovalbumin was used to immunize New Zealand rabbits and conduct the nasal cavity stimulation for preparing allergic rhinitis model.The rabbit behavior change was observed in the two groups.The turbinate and nasal septum mucosa were taken for preparing HE sections.The pathologic change of nasal mucosa was observed.The venous blood was collected for detecting CRLR and CGRP-RCP levels change by ELISA.Results The behavior scores showed that the behavior score on 1-5 d after nasal cavity stimulation had statistical difference between the two groups(P＜0.05).The HE staining displayed that the nasal cavity mucosa and mesenchyma had slightly edema with blood vessels mild hyperplasia and congestion,the inflammatory cells infiltration was unobvious,and had little nasal secretions.The group B had obvious interstitial edema,vascular hyperplasia,congestion and a large number of eosinophils and other inflammatory cells infiltration.The CRLR and CGRP-RCP levels in the group B were significantly increased compared with the group A(t=5.143,10.595,P＜0.05).Conclusion CRLR and CGRP-RCP may play an important effect in pathogenesis of allergic rhinitis.