1.Morphological characteristics of Chinese excellent men cyclists in 1-km time trials
Chinese Journal of Tissue Engineering Research 2010;14(11):2060-2063
BACKGROUND:Previous studies with regard to male track short-distance cyclists are concentrated on the descriptive and comparison studies of morphological characteristics of athletes Up to now,few studies report the relationships between morphological characteristics and sports performance in men 1-km cyclists.OBJECTIVE:To explore an effective morphological index for evaluating physical stamina level of men cyclists in 1-km time trials,and to establish morphological models and evaluation standards for men cyclists in 1-km time trials in China.METHODS:Totally 17 men who had prepared for the 1-km cycling time trials of the fifth cycling city sport meeting in Taiyuan were examined.With the methods of step by step regression,gray correlation,measurement and evaluation theory,the morphological characteristics of these excellent cyclists were explored,and utilized in establishing morphological models,single-grading table and the ranking evaluation criteria.RESULTS AND CONCLUSION:The typical indexes of excellent 1-km men cyclists were body height and fat-free body weight.The morphological characteristics of these excellent cyclists were as follow:tall,long leg and Achilles tendon;high foot arch;heavy body weight and fat-free body weight;as well as thick thigh The present study established the evaluation model of the morphological characteristics and evaluation standards for 1-km men cyclists
2.Clinical analysis of incomplete Beh et′s disease
Chinese Journal of Ocular Fundus Diseases 2003;0(06):-
Objective To investigate the clinical diagnosis and treatment of incomplete Beh et′s disease. Methods The clinical data of 52 patients (83 eyes ) with incomplete Beh et′s disease were retrospectively analysed. Visual acuity and results of examination of slit-lamp microscope, ocular fundus, and fundus fluorescein angiography (FFA) were observed and analysed. All of patients were treated by local cycloplegic, systemic treatment with glucocorticoid and cytoxan. The followed-up period was 1 year or more. Results Recurrent hypopyon or empyema of anterior chamber angle, and hyperaemia of optic papilla was found in 54 and 41 eyes, respectively. The results of FFA showed that abnormal changes were found in all of the eyes. There was vascular leakage and staining of optic papilla at the early and late stage in 83 eyes (100%), diffuse leakage of retinal capillary vessel in 83 eyes (100%), and leakage of affected branch of retinal vessel and staining of the vascular wall at the late stage in 71 eyes (85.54%).After the treatment, the disease recovered completely in 20 patients (26 eyes), recovered partly in 20 (33 eyes), and alleviated in 12 (24 eyes). No patients suffered from hypopyon or empyema of anterior chamber angle and hyperemia of optic papilla. None of the patients had oral ulcer, genital ulcer or arthritis until the last day of the follow-up. Conclusions Recurrent hypopyon, papillitis, and retinal vasculitis were the main clinical manifestations of incomplete Beh et′s disease. FFA redounds to the diagnosis of the disease and timely application of cycloplegic and systemic application of glucocorticoid and cytoxan may inhibit the aggravation of the disease effectively.
3.Chinese traditional medicine combined with western medicine treatment for proximal humerus malunited fracture
Orthopedic Journal of China 2006;0(14):-
[Objective]To investigate the clinical result of the Chinese traditional medicine combed with western medicine treatment for proximal humerus malunited fracture.[Method]Twenty-nine patients including 24 men and 5 women with malunion of proximal humerus were treated with the locking proximal plate.The average age of patients was 41.6 years old(from 21 to 53 years old).According to Neet classification on X ray,20 cases were two-part fractures and 9 cases were three-part fractures.These patients were treated by combination of Chinese traditional and western medicine,with the help of the functional training.All patients with proximal humerus malunited fracture were treated by operations with locking proximal humerus plate.A herbal bath was used to bathe after surgery.[Result]All 29 cases were available for follow-up.The average time for bony union on X ray was 9.6 weeks(from 8 to 12 weeks).Nonunion was not found in all cases.According to the Neer scoring system,there were 23 excellent cases,4 good case and 2 fair case.The excellent and good rate was 93.1%.[Conclusion]In dealing with proximal humerus malunited fracture,the advantages of the locking proximal humerus plate are minimally invasive with reliable fixation,few complication and the high bone union rate.A herbal bath can fasten recovering the function of the shoulder.Chinese traditional medicine combined with western medicine treatment can make satisfactory result and it is an effective method to recover the function of the shoulder.
4.Surveillance of malaria in Pukou District, Nanjing City, 2003 -2007
Chinese Journal of Schistosomiasis Control 2009;21(6):549,562-
A total of 10 local malaria cases and 21 imported cases were detected in Pukou District of Nanjing City from 2003 to 2007. The result suggests the surveillance of mobile population should be strengthened in this district.
5.The research of the analgesic effects by acupuncture at jiaji points in rat of incisional pain
Chongqing Medicine 2015;(18):2464-2466,2470
Objective To observe the effect of the application of acupuncture Jiaji points and continuous acupoints infusion of analgesic drugs at Jiajji in the treatment of postoperative pain on rat behavior through the establishment of a rat model of incisional pain .Methods 60 healthy male SD rats ,were randomly divided into sham control group ,PI group ,CSI group ,EA group and CAI group ,12 in each group .Plantar incision pain model in rats was established according to the method of Brennan ,rats in 5 groups at preoperative 1 h and 6 ,24 ,48 h after operation ,mechanical withdrawal threshold(MWT) and thermal withdrawal latency(TWL) were evaluated .Results Changes in MWT :5 groups of rats with experimental basis of mechanical withdrawal threshold values showed no significant difference among them ;comparison of the mechanical withdrawal threshold in the sham control group at each time point showed no significant difference(P>0 .05);when compared with sham control group ,MWT in PI group ,CSI group ,EA group and CAI group decreased significantly at 6 ,24 ,48 h after operation (P<0 .05);when compared with PI group ,MWT in CSI group ,EA group and CAI group increased significantly at 6 ,24 ,48 h after operation(P<0 .05);when compared with CSI group and EA group ,MWT in CAI group increased significantly at 6 ,24 ,48 h after operation (P<0 .05) .Changes of TWL :when compared with the sham control group ,TWL decreased significantly in PI group at 6 ,24 ,48 h after operation (P< 0 .05);when compared with the sham group ,TWL in CSI group ,EA group ,CAI group increased significantly at 6 ,24 ,48 h after operation (P<0 .05);when compared with CSI group and EA group ,TWL in CAI group increased significantly at 6 ,24 ,48 h after operation (P<0 .05) . Conclusion Electroacupuncture and continuous acupoint infusion of analgesic drugs at Jiaji could produce obvious analgesic effect , the analgesia effect of continuous acupoint infusion at Jiaji is better than subcutaneous analgesia .
7.Ultrasound-guided pus aspiration for the treatment of appendiceal abscess
Qiang WANG ; Hong ZHOU ; Liqun JIA
Chinese Journal of General Surgery 2000;0(12):-
ObjectiveTo evaluate the effect of ultrasound guided pus aspiration for appendiceal abscess.MethodsPercutaneous puncture and pus aspiration was performed under guidance of B US on 181 patients with appendiceal abscess, result was compared with 23 patients treated conservatively.ResultsCompared with that of control group, in pus aspiration group abdominal pain lasted 5 5 days shorter,fever lasted 5 days shorter,tenderness did 6 days shorter, cost was 2?356 yuan less and hospital stay shortened by 6 days.ConclusionPus aspiration under B US guidance is a safe,simple,less cost and effective treatment for appendiceal abscess larger than 1?cm?2?cm in children patients.
8.Measurement of rabbit marrow mesenchymal stem cells isolated and observation on their biological characteristic in vitro
Qiang ZHOU ; Qihong LI ; Jianzhong XU
Journal of Third Military Medical University 2003;0(16):-
Objective To establish the measurement for evaluating the efficiency of isolating and harvesting marrow mesenchymal stem cells (MSCs) and observe the main biological characteristic of MSCs in vitro during the passage cultivation. Methods ①The marrow nucleated cells in young rabbit marrow were isolated by the gradient centrifugation and cultivated to isolate and harvest MSCs. The efficiency of isolating and harvesting MSCs was measured by the number of the first passage MSCs from every 10~(6) of the marrow nucleated cells. ②The changes of MSCs morphology, growth and proliferation, synthesis of collagen typeⅠand activity of alkaline phosphatase (ALP) were observed during the passage cultivation. Results ① After cultivated at 2.5?10~(5)/cm~(2) marrow nucleated cells for 9-10 d, the first passage MSCs were harvested. The number of the first passage MSCs had a remarkable statistical positive correlation with the number of the marrow nucleated cells from one rabbit(r=0.932, P
9.Alteration of intraocular structure before and after vitrectomy
Guang-Ming, ZHOU ; Liang, YAN ; Qiang, WU
International Eye Science 2015;(8):1335-1339
AIM:To explore the possible alteration of refraction and estimate the potential impact of visual quality, investigate the impact of intraocular structure between the simple operation and the combination of phacoemulsification and intraocular lens implantation, and observe the influence among various tamponade materials application.
METHODS: Ultrasound biomicroscopy ( UBM ) and intraocular len ( IOL ) Master were measured for simple vitrectomy patients ( the simple group, 37 eyes ) and vitrectomy combined with cataract surgery patients ( the combined group, 11 eyes ) . Several parameters were measured such as the anterior chamber depth ( ACD ) , angle opening distance ( AOD ) , trabecular iris angle (TIA), axial length (AL), et al . The simple group was further divided into three sub - groups by various tamponade materials in vitreous: silicone oil group, gas group and equilibrium liquid group. All patients were measured including ACD, AOD250, AOD500, TIA500, AL and corneal curvature (K1, K2) parameters preoperatively and 2wk postoperatively.
RESULTS: AOD250, AOD500, TIA500 and AL were decreased 0. 13±0. 21mm, 0. 16±0. 22mm, 8. 68o±9. 72o and 0. 18±0. 24mm ( all P<0. 05 ) respectively in the simple group postoperatively. ACD, AOD250, AOD500, TIA500 and AL in combined group increased 0. 95±0. 54mm, 0. 13±0. 12mm, 0. 22±0. 20mm, 11. 49o±11. 06o, and 0. 45±0.30mm ( all P < 0. 05 ) respectively after operation. Alteration of ACD, AOD250, AOD500, TIA500 and AL were different between simple group and combined group ( P<0. 05). Of data from various tamponade materials sub-groups, ACD, AOD250, AOD500 and TIA500 of the gas group reduced 0. 19±0. 17mm, 0. 14±0. 09mm, 0. 20±0. 12mm, and 12. 02o±6. 64o respectively after operation ( P<0. 05), the AOD250, AOD500, TIA500 and axial length of silicone oil group decreased 0. 19±0. 25mm, 0. 21±0. 26mm, 10. 44o±9. 67oand 0. 23±0. 19mm respectively after operation ( P < 0.05 ) , while equilibrium liquid group had no significant changed in all intraocular structure (P>0. 05). However, neither the corneal thickness nor curvature (K1, K2) were changed postoperatively (P>0. 05).
CONCLUSION:Alteration of anterior segment and axial length in combined group are different from that of the simple group, while the corneal thickness and corneal curvature ( K1, K2 ) are not obviously changed in all groups. Meanwhile, various tamponade materials can also cause different impact on intraocular structure and refraction. In brief, alterations of intraocular structure might cause discrepant refractive changes and potential influence of visual quality.
10.The competitive inhibitory effects of HLA-DRB1-binding peptide on T cell activation
Yongjing CHENG ; Qiang ZHOU ; Zhanguo LI
Chinese Journal of Immunology 2001;0(10):-
Objective:To evaluate the impact of altered collagen Ⅱ(CⅡ) peptide(S268-270) on T cell activation.Methods:Altered CⅡ263-272 with G268,P269 and K270 consecutively substitution with A or G were synthesized using solid phase various concentrations were added to HLADR1 transfected APC.Expression of FITC stainning was analyzed by fluorescence-activated cell sorting,and the binding of altered CⅡ peptide to HLA-DR1 molecule on cell membrane was evaluated.Irridiated HLA-DR1 expressing APC was incubated with CⅡ263-272 or hemagglutinin(HA)306-318 and altered CⅡ peptides at various concentrations for 2 hours before T cells(3.19) were added.Supernatant was harvested and then added to IL-2 dependent CTLL cell.The cell proliferation was examined by methotetrazolium(MTT) method.Results:The altered CⅡ peptide and wild type of CⅡ263-272 were able to competitively bind to HLA-DR1 molecule expressed on cell membrane of transfected APC.CⅡ or HA induced T cell activation was significantly inhibited by the altered peptide S268-270.Conclusion:Altered CⅡ263-272 with G268,P269 and K270 consecutively substitutions with A or G inhibited CⅡ263-272 and HA306-318 induced T cell activation through competitively binding to HLA-DR1,suggesting a new approach in inhibition of T cell activation in RA.