Objective:To analyze relationship between early intervention treatment and long-term prognosis in pa-tients with acute non-ST segment elevation myocardial infarction (NSTEMI).Methods:A total of 948 acute NSTE-MI patients were enrolled.According to NSTEMI intervention treatment timing,they were divided into early inter-vention group (received intervention treatment within 48h n=411,)and late intervention group (received interven-tion treatment after48h,n=537).According to TIMI risk score,the two groups were further divided into low risk subgroup,medium risk subgroup and high risk subgroup,the clinic long-term outcome was compared among all groups.Results:Compared with late intervention group,during one year follow-up,there were significant reduction in mortality during hospitalization (4.8% vs.2.2%)and incidence rate of major adverse cardiovascular events (MACE,13.4% vs.10.0%)in early intervention group,P <0.01 both;in early intervention group,mortality dur-ing hospitalization of low and medium risk patients was significantly lower than that of high risk patients (3.3% vs. 8.9%,P <0.01).Multi-factor regression analysis indicated that aged,high Killip class,reduced left ventricular e-jection fraction,high TIMI risk score and late intervention treatment were independent risk factors for clinic long-term outcome in NSTEMI patients (OR=1.027~2.079,P <0.05 or <0.01).Conclusion:Early intervention treat-ment can improve prognosis in NSTEMI patients.

Bile Duct Neoplasms ; surgery ; Bile Ducts ; pathology ; surgery ; Bile Ducts, Intrahepatic ; pathology ; surgery ; Biliary Tract Diseases ; surgery ; Biliary Tract Surgical Procedures ; methods ; trends ; Cholecystectomy, Laparoscopic ; methods ; trends ; Cholelithiasis ; surgery ; Humans

Anti-Bacterial Agents ; therapeutic use ; Endophthalmitis ; complications ; drug therapy ; Humans ; Infant, Newborn ; Meningoencephalitis ; complications ; drug therapy ; Suppuration

Hearing ; Hearing Aids ; Prostheses and Implants

Carotid Body Tumor ; genetics ; Humans ; Paraganglioma ; genetics

Objective: To assess the clinical effect of modified Mapleson D breathing system in intravenous anesthesia for minor surgical procedures. Methods: A total of 112 patients (ASA I-II) scheduled for minor outpatient surgery were randomly divided into two groups, with 56 in each group. Patients in Group A inhaled oxygen using common face mask, and were transferred to artificial ventilation by emergency ventilator when respiratory depression occurred; those in Group B were subjected to modified Mapleson D breathing system, and were transferred to artificial ventilation using the present breathing system when respiratory depression occurred. The respiratory rate (RR), oxygen saturation (SpO 2), Partial end-tidal CO2 pressure (PETCO 2), occurrence (T1) and rectification time (T2) of respiratory depression were all recorded in the two groups. Results: The incidence of respiratory depression in Group B was lower than that in Group A; moreover, Group B also had a higher incidence of hyoxemia and shorter rectification time compared with Group A. Conclusion: Compared with conventional face mask, our modified Mapleson D breathing system is easy and convenient to use, and it leads to lower incidence and quick rectification of respiratory depression, showing a potential in the airway management of patients undergoing minor intravenous anesthesia.

Objective: To establish a mouse hepatic cancer cell line Hca-F transfected with shRNA (small hairpin RNA) targeting annexin A7, so as to provide a basis for future study. Methods: Three shRNAs(shRNA1, 2 and 3) were designed and inserted into the pSilencer vector to silence annexin A7 gene. The three pSilencer-shRNA vectors were transfected into Hca-F cells separately, and the most effective pSilencer-shRNA vector was selected based on the results of RT-PCR and Western blotting. The Hca-F cells were transfected with the most effective pSilencer-shRNA vector and the transfectants were selected by 400 μg/ml G418. The cells with annexin A7 stably knockdown were passaged and the expression of annexin A7 was confirmed by Western blotting, and the result was compared with those transfected with empty vector and normal controls. Results: The sequencing results confirmed that the sequences of the 3 shRNAs were correct,and shRNA1 was found to have the best inhibitory effect against annexin A7. Compared with normal Hca-F cells and those transfected with empty vectors, the annexin A7 protein expression was significantly down-regulated in cells transfected with pSilencer-shRNA(0.318 6 vs 0.824 3, 0.798 7,P <0.05), with no significant difference found between the former 2 groups. Conclusion: We have successfully established a Hca-F cell line with annexin A7 stably down-regulated using shRNA technique, paving a way for future study.

Objective: To study the effect of obstructive jaundice on liver function and pathomorphology in rats. Methods: Sixty healthy male Sprague-Dawley rats were randomly divided into bile duct ligation (BDL) group (the common bile ducts were ligated, n = 50) and sham group(the common bile ducts were isolated but not ligated, n=10). According to the ligation periods, BDL group was further divided into BDL3, BDL7, BDL14, BDL42 and BDL42 groups. Liver function indices, including the serum total bilirubin(TBIL), direct bilirubin(DBIL), total bile acid(TBA), and alanine aminotransferase(ALT), were measured; and the liver pathomorphology was observed 3, 7, 14, 28, and 42 days after BDL. The above examinations were performed in the sham rats 7 days after operation. Results: The liver function of rats had obvious changes compared with that of sham group. The serum levels of TBIL, DBIL, and TBA reached the peak in the early phases (BDL4 or BDL7) and decreased gradually thereafter, but were still higher than that of the sham control in the end phase(BDL42). There was a similar change in the biochemical indices such as ALT. The main pathomorphology changes in BDL group were intrahepatic bile duct proliferation and hepatic fibrosis. Conclusion: There is obvious impairment in the early phase of obstructive jaundice: the liver function may be improved to a certain degree by a compensation mechanism: however 9 the persistence of obstructive jaundice finally leads to hepatic fibrosis. The liver function indicated by the pathomorphology changes can not reflect the actual impairment of liver tissue in obstructive jaundice rats.

Objective: To investigate the relationship of mitochondrial DNA 4977 bp (mtDNA 4977) deletion in the peripheral blood with severity and stability of coronary atherosclerosis. Methods: We selected 90 unrelated patients with coronary atherosclerotic heart disease (CAD) who were diagnosed by coronary angiography (CAG). The severity of pathological changes of the coronary artery was assessed by the number of diseased coronary branches and Gensini score. The CAD patients were further divided into subgroups according to the clinical types, the number of diseased coronary branches and Gensini score. Control group included 60 healthy age-matched subjects. The relative amount of mtDNA 4977 deletion was determined using a nested polymerase chain reaction (PCR) protocol. White blood cell (WBC) count, high sensitive C reactive protein (hsCRP), lipids (TC, TG, LDL-C, HDL-C), plasma glucose (FPG, 2hPG), blood pressure (SBP, DBP) and body mass index (BMI) were all measured. The information on age, sex and medical histories, including smoking status, hypertension and diabetes mellitus, were obtained in all subjects. Clinical parameters, biochemical indicators, the incidence and relative amount of mtDNA 4977 deletion were compared between the subgroups; the correlation coefficients of mtDNA 4977 relative amount with WBC count, hsCRP and other conventional risk factors for CAD were calculated. Results: The incidence and relative amount of mtDNA 4977 deletion in the peripheral blood in CAD patients were significantly higher than those in the controls (P<0.01). No significant differences were found in the incidence and relative amount of mtDNA 4977 deletion between patients with angina pectoris and acute myocardial infarction (P>0.05). MtDNA 4977 deletion incidence and relative amount increased with the increase of diseased coronary branches and Gensini score. In CAD patients mtDNA 4977 deletion relative amount was positively correlated with the number of diseased coronary branches and Gensini score (P<0.01), not correlated with WBC count and hsCRP. Conclusion: Peripheral blood mtDNA 4977 deletion can be used to predict the severity of coronary atherosclerosis, though it is not associated with the stability of pathological changes of the coronary artery.

Objective: To investigate the effect of Scutellaria barbata D. Don on the expression of ADAM10 (a disintegrin and metalloproteinase 10) in primary liver cancer tissues in rats. Methods: Total of 120 Wistar rats were randomly divided into four groups: normal, model, matrine and Scutellaria barbata D. Don groups. The model of rat bearing primary liver cancer was induced by diethylnitrosamine. The rats in the matrine group and Scutellaria barbata D. Don group were fed with matrine and Scutellaria barbata D. Don powder, respectively. The rats in the normal group and model group were fed with 0.9% sodium chloride solution. The number of hepatocellular carcinoma nodules was calculated, and the tumor growth inhibition rate was also calculated. The pathologic changes of hepatic tissues in rats of each group were observed by hematoxylin and eosin staining. The expressions of ADAM10 in liver cancer tissues in these four groups were determined by immunohistochemistry and Western boltting. Results: The number of hepatocellular carcinoma nodules of the matrine group and Scutellaria barbata D. Don group was less than that of the model group (P < 0.05) with the appearance of apoptotic bodies. The tumor growth inhibition rate of Scutellaria barbata D. Don group was higher than that of the matrine group (P < 0.05). The positive expression rate and the expression level of ADAM10 in liver cancer tissues of the model group were significantly higher than those of the normal group (P < 0.05). The positive expression rate and the expression level of ADAM10 in liver cancer tissues of matrine group and Scutellaria barbata D. Don group were lower than those of the model group (P < 0.05). The positive expression rate and the expression level of ADAM10 in liver cancer tissues of Scutellaria barbata D. Don group were lower than those of matrine group (P < 0.05). Conclusion: Scutellaria barbata D. Don may inhibit the growth of primary liver cancer in rats through down-regulating the expression of ADAM10. Copyright © 2013 by TUMOR.