Objective:To explore the diagnostic value of ABI detection in lower limb vasculopathy of diabetic patients.Methods:ABI detection was performed in 44 cases suspected of diabetic lower limb vasculopathy,and the results were compared with that of DSA. Results:There was no statistically significant difference between ABI detection and DSA in the diagnosis of diabetic lower limb vasculopathy.Compared with DSA,the accordance rate,sensitivity and specificity of ABI were 87.31%,86.46%and 82.00%respectively. Conclusion:By ABI detection,lower limb vasculopathy of diabetic patients can be detected.

The neurologic function and cerebral vascular anatomy of non-human primate are closely resemble to those of human, so non-human primate cerebral ischemic model is a particularly useful tool in studying the damage mechanism and therapy intervention of cerebrovascular disorder. The authors reviewed the establishment method and neurological behavioral assessmental method of non-human primate cerebral ischemic model.

effects of tachykinin SK, SP on mouse splenocyte and thymocyte have been studied.It was found that SK and SP can stimulate ConA or LPS-induced splenocyte prolifertion and antibody production (in cluding IgG, IgM, IgA).5 ?10~(-7)M/L SK can stimulate ConA-induced thymocyte proliferation but SP can't. The subsets of T lymphocytes in spleen and thymus have been changed after SK treatment by FACS analysis. These results show that SK and SP may play a significant regulatory role in immune response.

Objective To examine the expression of transforming growth factor β(TGF-β) activated kinase 1 (TAK1) in esophageal organization and the impact of TAK1 expression on clinicopathologic data. Methods Specimens from 80 patients with esophageal adenocarcinoma managed in our hospital were included in this study. Immunohistochemical staining was used to detect the expression of TAK1 in 80 cases of esophageal squamous cell carcinoma tissues and 80 cases of normal esophageal mucosal tissues. All the specimens were confirmed by pathology for esophageal squamous cell carcinoma or esophageal normal tissues. Results The positive expression rate of TAK1 in esophageal cancer organizations and normal esophageal mucosal tissues were 80% and 11.25%, respectively. The positive expression rate of TAK1 in esophageal cancer were significantly higher than those in normal esophageal mucosal tissue (P < 0.05). The positive expression rate of TAK1 in esophageal cancer organizations has nothing to do with the age, sex, tumor differentiation degree and size (P>0.05), but with lymph node metastasis and clinical stage (P<0.05). Patients with positive TAK1 expression had significantly lower five-year survival rate than those with tumors having positive TAK1 expression Conclusion TAK1 played an important role in the pathology and development of squamous cell carcinoma , and could be an important therapeutic target in the treatment of esophageal cancer.

OBJECTIVE: To isolate and purify radix hedysari polysaccharide 3 (HPS-3) and determine the monosaccharide composition and the contents of polysaccharide in its four components. METHODS: Radix Hedysari polysaccharide was extracted by water, precipitated with ethanol, treated with Sevag method, decolored with H2O2, then precipitated with ethanol and dialyzed. By using DEAE-cellulose 52 and Sephadex G-100, HPS-3-A, HPS-3-B, HPS-3-C and HPS-3-D were purified. Their purity were determined by high performance gel permeation chromatography (HPGPC). By TLC and GC, the monosaccharide composition of four components was analyzed, and then the modified phenol sulfuric acid method was used to determine the content of polysaccharide of the four fractions. RESULTS: HPS-3-A was composed mainly of glucose, and the content of polysaccharide was 99.2%. But HPS-3-B, HPS-3-C and HPS-3-D were all composed mainly of arabinose and galactose. The contents of polysaccharide of them were 96.5%, 95.3% and 95.2%, respectively. CONCLUSION: This study provides the foundation for the research of structure-function relationship of Radix Hedysari polysaccharide 3. Copyright 2012 by the Chinese Pharmaceutical Association.

Objective To explore the diagnosis and treatment of cytomegalovirus (CMV) pneumonia in patients who received immunosuppressive therapy after renal transplantation. Methods 15 patients out of the 110 recipients of renal transplantation were diagnosed as to have CMV pneumonia, during a period of the 2nd to 4th month after renal transplantation. All the 15 patients were treated with a systematic strategy, including rational use of antibiotics, withdrawal of immunosuppressant drugs and rebuilding the body′s immunity function, timely application of mechanic ventilator, and continuous high volume hemofiltration (CVVHF). The clinical responses of the 15 patients were reviewed and retrospectively analyzed. Results 15 recipients contracted CMV pneumonia out of 110 cases (13.6%), and it occurred on a mean of 93.3 days after renal transplantation. All of them were febrile with cough, and showed manifestations of interstitial pneumonia on chest films. CMV-IgM were positive in 13 cases, CMV DNA positive in 9 cases, and 4 patients presented signs of acute respiratory distress syndrome. The condition developed quickly in all the patients. 9 patients recovered and 5 patients died, including one who gave up treatment for financial reason. Conclusion CMV infection is a serious complication after renal transplantation. Early diagnosis with detection of CMV DNA and serum antibody of CMV-IgM should be emphasized. The comprehensive protocol principally composed of anti-virus therapy with cymevan or ganciclovir, monitoring of peripheral lymphocyte subgoups, and adjustment of dosage of immunosuppressive agent may improve recovery rate of CMV pneumonia.

Objective To investigate effect of p27kip1 gene transfer mediated by adenovirus vector on the cell cycle of esophageal carcinoma cells. Methods The recombination adenoviral vector carrying p27kip1 gene (Ad-p27kip) was transfected to esophageal carcinoma Eca9706 cells. The effect of p21kip1 gene transfer on the cell growth suppression was measured by MTT method. Expression of p27kip1 was detected by immunocytochemical technique. Cell cycle was analysed by flow cytometry(FCM). Results After Eca9706 cells were transfected with Ad-p27kip1, p27kip1 expression increased, the cell growth was significantly inhibited and the ratio of G 0/G 1 phase cells increased and the cell number of G 2/M and S phases decreased. Conclusion p27kip1 gene transfer mediated by adenovirus can significantly inhibit the growth of esophageal carcinoma cells and cause G 1 phase blocking.

Animals ; Corticosterone ; pharmacology ; Hippocampus ; drug effects ; physiology ; In Vitro Techniques ; Long-Term Potentiation ; drug effects ; physiology ; Male ; Rats ; Rats, Wistar

Objective To investigate the relationship between the p27~(kip1) expression and the change of radiosensitivity of esophageal cancer cells.Methods Radio-resistant cells (EC9706R) were gradually isolated by means of repeated gamma-ray irradiation (6 000 cGy in total) upon human squamous esophageal carcinoma cells (EC9706).The radiosensitivity of these two types of cells was measured by standard colony formation assays,their cell cycle distribution analyzed by flow cytometry respectively,and the p27~(kip1) expression detected by immunocytochemistry.Results As compared with their parent cells,the isolated human squamous esophageal carcinoma cells(EC9706) showed clearly greater radio-resistance(for EC9706R,SF_2=65.71%,D_0=2.20 Gy,D_q=1.61 Gy,N=2.07,and for EC9706,SF_2=46.72％,D_0=1.61 Gy,D_q=0.99 Gy,N=1.85;SF_2,D_0,Dq and N were all higher).As to the cell cycle distribution,the population of G_1 phase cells in EC9706R cells was significantly decreased,but the proportion of S phase cells was significantly increased as compared with the parent cells.Immunocytochemistry revealed that the p27~(kip1) expression of EC9706R cells was clearly lower than that of EC9706 cells.Conclusion Cell phase change due to the decrease of p27~(kip1) expression may be one of the mechanisms of the generation of radio-resistance in esophageal cancer cells.

Objective To investigate the risk factors of diabetic foot by observing the changes of the relative index. Methods 134 pa-tients were randomly divided into non-diabetic foot (NDF) group and diabetic foot (DF) group,the changes of blood glucose,HbA1c,creati-nine,uric acid,microalbuminuria and the complications of diabetes were observed. Results There no difference in blood glucose level be-tween 2 groups (P>0. 05). The HbA1c levels of DF group was higher,with a significant difference(P<0. 05). The levels of uric acid in fe-male of DF group was higher than those in female of NDF group(P<0. 05). there was significant differernces in levels of microalbuminuria (P<0. 05),but no statistic difference in levels of potassium and sodium between 2 groups. Conclusion The changes of HbA1c,uric acid and microalbuminuria could indicate the diabetic foot in clinic.