Objective:To study the protective effect of self-designed Shanghai multi-organ preservation solution(SMO) on mitochondria function in canine kidney cortex during cold storage.Methods: Canine kidneys were perfused and stored with cold(0-4℃) SMO,HC-A and UW solution (the later 2 as control) separately.Ultrastructure alteration of mitochondria was observed by electron microscopy.Mitochondria were isolated from cortex homogenization by differential centrifugation and their respiratory function was measured polarographically with Clark oxygen electrode.The four-state curve of mitochondria respiration was drawn and the state Ⅲ and Ⅳ oxygen consumption rate,the respiration control proportion(RCR) and phosphatasel/oxygen ratio(P/O) were calculated.Results: We found that the mitochondria swelling and membrane disruption in HC-A group were more severe than those in SMO and UW group.The rate of oxygen consumption in state Ⅲ,RCR and P/O were obviously decreased in all groups with the prolongation of cold storage time.The rate of oxygen consumption in state Ⅲ in HC-A group was lower compared to that in SMO group during 1-3 d cold storage(P

Objective:To observe the influence of a self-designed multiple organ preservation solution,namely Shanghai multi-organ preservation solution(SMO),on the apoptosis of canine renal cortical cells during cold storage and the related mechanism.Methods: Canine kidneys were freshly perfused and separately stored with cold(0-4℃) SMO,hypertonic adenine citrate solution(HC-A),and University of Wisconsin solution(UW solution)(the latter 2 as control).At defined time points during storage,the morphologic changes of renal cortical samples were observed;apoptosis of renal cortical cell was determined by TUNEL;changes of malonldialdehyde(MDA) in cortex homogenate were measured by thiobarbituric acid method;and the activity of superoxide dismutase(SOD) was determined by xanthine oxidase method.Results: Pathologic assessments indicated that the kidney damage was similar in 3 groups within 24 h.The kidney tubular cell swelling and necrosis in SMO group and UW group were slighter than those in HC-A group after stored for 2 days.With the prolongation of storage time,apoptosis increased in all groups,with the apoptotic index of SMO group significantly lower than that of HC-A group(P

UW solution has bean considered the gold standard for abdominal organ preservation. Celsior solution and HTK solution, with low potassium and low viscosity, are becoming more widely used in the preservation of the kidney, liver and pancreas, and are gradually accepted as an ideal alternative for UW solution. Recent studies have found that addition of trophic factors can attenuate hypothermic ischemic injury to the donor organ, thus improving the preservation outcome. This paper reviews the progress in laboratory research and looks foward to the clinical application of multi-organ preservation solution.

? AlM: To investigate the therapeutic effects of Pranoprofen on tear dynamic abnormity induced by conjunctivochalasis ( CCh) .
?METHODS: Seventy cases ( 70 eyes ) whose the main symptoms was epiphora induced by CCh were divided into II, Ⅲ and Ⅳ in 3 groups according to Zhang’s grading standards, meanwhile, all the patients were treated with the eye drops of Pranoprofen for 2wk. After that, the curative effect of each group was analyzed before and after treatments according to the symptoms, dye disappearance test, bitter taste test and tear break-up time.
?RESULTS: After 2wk treatment of Pranoprofen, the symptom of patients at grade I and II improved obviously grade Ⅲ improved more, grade Ⅳ did little. The positive rate of dye disappearance was the highest in grade II, the second in grade Ⅲ, and the third in gradeⅣ. There was significant difference among the 3 groups (P<0. 05). The time of bitter taste test was shortened (P<0. 05) and the tear break-up time was prolonged (P<0. 05) in grade II after treatment, however, the time of those in gradeⅢandⅣhad not changed than before ( P>0. 05).
?CONCLUSlON: Pranoprofen has good effects on tear dynamic abnormity induced by grade II of cch.

Objective: To study the protective effect of self-designed Shanghai multi-organ preservation solution(SMO) on mitochondria function in canine kidney cortex during cold storage. Methods: Canine kidneys were perfused and stored with cold (0-4 °C) SMO, HC-A and UW solution (the later 2 as control) separately. Ultrastructure alteration of mitochondria was observed by electron microscopy. Mitochondria were isolated from cortex homogenization by differential centrifugation and their respiratory function was measured polarographically with Clark oxygen electrode. The four-state curve of mitochondria respiration was drawn and the state III and IV oxygen consumption rate, the respiration control proportion(RCR) and phosphatase1/oxygen ratio(P/O) were calculated. Results: We found that the mitochondria swelling and membrane disruption in HC-A group were more severe than those in SMO and UW group. The rate of oxygen consumption in state III, RCR and P/O were obviously decreased in all groups with the prolongation of cold storage time. The rate of oxygen consumption in state III in HC-A group was lower compared to that in SMO group during 1-3 d cold storage(P<0.05). The rate of oxygen consumption in state IV was slightly increased during the cold storage, but there was no statistical difference between HC-A and SMO group. RCR and P/O in SMO group were statistically higher than those in HC-A group (P<0.05) and were similar to those of UW group. Conclusion: SMO solution has similar protective effect on cortex mitochondria function impairment induced by hypothermia and ischemia during cold storage as UW solution, and the effect is more potent than that of HC-A solution.

Objective: To observe the influence of a self-designed multiple organ preservation solution, namely Shanghai multi-organ preservation solution (SMO), on the apoptosis of canine renal cortical cells during cold storage and the related mechanism. Methods: Canine kidneys were freshly perfused and separately stored with cold (0-4°C) SMO, hypertonic adenine citrate solution (HC-A), and University of Wisconsin solution (UW solution) (the latter 2 as control). At defined time points during storage, the morphologic changes of renal cortical samples were observed; apoptosis of renal cortical cell was determined by TUNEL; changes of malonldialdehyde (MDA) in cortex homogenate were measured by thiobarbituric acid method; and the activity of superoxide dismutase (SOD) was determined by xanthine oxidase method. Results: Pathologic assessments indicated that the kidney damage was similar in 3 groups within 24 h. The kidney tubular cell swelling and necrosis in SMO group and UW group were slighter than those in HC-A group after stored for 2 days. With the prolongation of storage time, apoptosis increased in all groups, with the apoptotic index of SMO group significantly lower than that of HC-A group (P<0.05). The levels of cortical MDA increased with storage time, and that in SMO group was significantly lower than that in HC-A group (P<0.05). The activities of cortical SOD were similar in the 3 groups. Conclusion: SMO solution can improve the efficiency for protection of renal cortex, which might be associated with the decrease of cells apoptosis and inhibition of lipid peroxidation injury.

Background Pigmentary glaucoma and pseudoexfoliation glaucoma are characterized by pigment dispersion in trabecular meshwork,and the dipersitional pigment probably contributes to the resistance of aqueous outflow pathway,irreversible damage of the trabecular meshwork and the remodeling abnormality of extracellular matrix.Researches determined that contents of transforming growth factor-β (TGF-β) in the aqueous humor are increased in glaucomatous eyes.However,the effects of TGF-β and fibronectin (FN) in the trabecular meshwork cells (TMCs) acted by iris pigmentary particles still are not elucidated.Objective This study was to investigate the effects of iris pigment particles on TGF-β1 and FN expression in bovine TMCs (BTMCs) cultured in vitro.Methods BTMCs were cultured in vitro by explant culture method and identified by morphological evaluation.The third generation of BTMCs were divided into normal control group and pigment group,and 100 μ1 PBS and 100 μl iris pigment suspension (final concentration of 1 × 107 particles/ml) were added into the medium for 24 hours,respectively.The expressions of TGF-β1 mRNA,FN mRNA and their proteins in the BTMCs were assayed by real-time fluorescence quantitative PCR and ELISA,respectively.Results Cultured cells grew well and showed the fusiform,polygon and dendritic like in shape,with pigmented and round nuclei.The relative expression levels of TGF-β1 mRNA and FN mRNA in the cells were 2.98±0.27 and 0.36±0.10 in the iris pigment group,which were significantly higher than 1.00±0.00 and 1.00±0.00 in the normal control group (t =12.68,10.60,both at P =0.00).The concentrations of TGF-β1 protein and FN protein in the cell suspension were (156.60±9.74)ng/L and (59.29±15.79)mg/ml in the iris pigment group,showing significant differences in comparison with (65.46 ± 14.24) ng/L and (102.10 ± 12.14)mg/mlin the normal control group (t=9.15,P=0.00;t=3.72,P=0.02).Conclusions The expression of TGF-β1 is up-regulated and that of FN is down-regulated in BTMCs cultured by iris pigment,inferring that TGF-β1 and FN participate in the pathogenesis and development of pigmentary and pseudoexfoliation glaucoma.

Objective To study the influence of systematic respiratory training of patients with chronic ob- structive pulmonary disease(COPD)on their off-respirator pulmonary function and emotional depression.Methods Sixty COPD patients were divided into a treatment group and a control group.The patients in both groups were given routine treatment,while those in the treatment group were also given respiratory training in addition for 9-12 months after ceasing the mechanical ventilation.The index of pulmonary function such as FEV1 and depression(SDS score) were evaluated before and after the respiratory training.All data was analyzed by using SPSS 10.0.Results The FEVI of all patients was improved after 9-12 months of respiratory training(P

Objective To study the characteristics of plasma adiponectin levels exhibiting in gestation women and newborns, as well as the relationships between adiponectin levels and fetal birth weight. Methods Totally 98 subjects have been considered in this study, in-cluding venous blood samples of 36 healthy non-pregnant women (control group), 31 uncomplicated pregnant women (pregnancies group) and the cord blood samples taken at delivery of their singleton infants born at term (newborns group). The concentrations of adiponectin and insulin were determined by radioimmunoassay technique. And SPSS was used for statistical analyses. Results The adiponectin level in women of late period of gestation (WLPG) (11.10±5.72)g/ml was lower than that of other two groups, and the newborns average cord plasma level of adiponectin was (30.71±12.77) g/ml significantly higher than that of the control group (16.52±6.87) g/ml. The adipone-ctin level for WLPG was correlated with FINS and HOMA-IR (r = -0.411, -0.393, P <0.05). The adiponectin level in cord plasma was positively correlated with birth weight(r = 0.416, P < 0.05). The adiponectin level in WLPG was correlated neither with the adiponec-tin level nor body weights of their newborns. Conclusions The average cord plasma adiponectin level of newboms is significantly higher than that of the control group and WLPG. It is positively correlated with birth weight, which suggests that adiponectin may be involved in reg-ulating fetal growth. The adiponectin level in WLPG is correlated neither with the adiponectin level nor body weights of their newborns.

Objective To set up a method of detecting platelet associated antibody(PA Ig) by flow cytometry(FCM),and to investigate the value of FCM method in the diagnosis of idiopathic thrombocytopenic purpura(ITP).Methods We detected the platelet associated antibody of 20 healthy donors and 10 patients with ITP.These samples were also measured with ELISA.The results of FCM were compared with the results of ELISA.Results By FCM,the measured value of PA IgG,PA IgA and PA IgM,from 20 normal dornors,were 2.56?2.08%、13.75?5.98% and 13.90?7.28%,respectively.In patients with ITP,PA IgG,PA IgA and PA IgM were 28.13?17.74%、25.07?16.84% and 23.22?17.85%.Conclusion FCM can be a rapid,sensitive and new method in detecting platelet autoantibody,and has better potential than ELISA.