Biomedical Research ; Carcinoma, Hepatocellular ; virology ; Hepatitis B virus ; genetics ; physiology ; Humans ; Liver Neoplasms ; virology

Objective:To establish the quality standard for Tangkening Granule.Methods:Gardenia,Gold Theragran and batatatis in Tangkening Granule were identifi ed by TLC,the content of geniposide was determined by HPLC.Result:The spots in TLC was clear,the negative control had no interference,the average recovery was 99.9%,RSD was 2.9%(n=6).Conclusion:The method was accurate,reproducible and specifi c.It can be used for quality control of Tangkening Granule.

Objective To evaluate the diagnostic values of location and qualitation of tendinitis of rotator cuff. Methods One hundred and sixty-two shoulders with shoulders pain or functional incapacity were performed ultrasonography and the sonograms were classified. Results Ultrasonic findings of these tendinitis were tendinitis of caput longum musculi bicipitis brachii in 39 cases, tendinitis of caput longum musculi bicipitis brachii in 33 cases, supraspinatus tendinitis in 51 cases, calcific supraspinatus tendinitis in 19 cases and bursitis of shoulder in 20 cases,especially. Conclusions Ultrasonography is a useful tool for diagnosis of tendinitis of rotator cuff in location and qualitation.

AIM:To clarify the impact of heart shock factor 4b (Hsf4b) K65R mutation on the regulation of downstream protein expression .METHODS:Non-functional Lys mutant plasmid pWZL-blast-HA-Hsf4b/K65R was genera-ted by replacing single , homologous amino acids using KOD-Plus-Mutagenesis-Kit.Mouse lens epithelial mLEC stable cell lines expressing Hsf4b or Hsf4b/K65R were constructed by lentivirus infection .The expression of Hsf4b in the mutant and the wildtype mLEC cells was confirmed by Western blotting .The expression of Hsf4b downstream proteins such as heat shock protein ( Hsp)70, Hsp90, Hsp27 and CryAB was examined by Western blotting and real-time PCR.RESULTS:The results of PCR and DNA sequencing confirmed the successful construction of mLEC Hsf 4b/K65R mutant.The K65R mutation didn’t influence Hsf4b expression in the mLEC cells.After K65R mutation in Hsf4b, the expression levels of Hsp27 and CryAB were down-regulated and the expression of Hsp 70i and Hsp90a upregulated.CONCLUSION: pWZL-blast-HA-Hsf4b/K65R can be used to construct a stable cell line by infecting with lentivirus .Hsf4b/K65R mutation influ-ences the regulation of downstream heat shock proteins .

Animals ; Humans ; Neoplasms ; metabolism ; pathology ; Signal Transduction ; physiology

In order to strengthen the management of disinfection quality of endoscopes, Quality Control Center of Digestive Endoscopy and Nosocomial Infection Control Center of Yunnan Province investigated the diagnosis, treatment, cleaning and disinfection conditions and disinfection quality of digestive endoscopes in some medical institutions of Yunnan Province by web questionnaire from April to May in 2019, and 277 valid questionnaires were finally obtained. SPSS 19.0 statistical software was used to analyze the influencing factors of cleaning and disinfection process and the infection control implementation of digestive endoscopes in 227 secondary and tertiary hospitals. The results showed that the number of decontamination people who had received systematic training in Yunnan Province was significantly lower than that in other areas of China. The hospital level, the number of decontamination personnel, and decontamination methods affected the implementation of cleaning and decontamination process and infection control, while the allocation of decontamination supplies had no effects. It is important to establish an effective mechanism for the normalized implementation of cleaning and disinfection of digestive endoscopes.

Objective To establish a method for rapidly detecting the G-protein ?3 subunit (GNB3) 825 site single nucleotide polymorphism (SNP) and to analyse the relationship between GNB3 825 site gene SNP and obesity. Methods The real-time fluorescent PCR was employed to analyse the GNB3 825 site gene SNP of 420 samples from 21 provinces and the the frequencies of genotypes were compared with those detected by gene sequencing. GNB3 825 site genotype, body weight, body mass index (BMI) and fat content were examined from 207 subjects and the correlation between GNB3 825 site gene SNP and obesity was analysed. Results The result by real-time fluorescent PCR showed that the frequencies of 825T and 825C haploid were 46.90% and 53.10%, respectively, and the frequencies of 825TT, 825TC and 825CC genotype were 22.38%, 51.42% and 28.10%, respectively, with no other genotype detected, which was consistent with the result by gene sequencing. BMI and fat content were significantly higher in subjects with GNB3 825TT than in subjects with other genotypes. Body weight was much higher in subjects with GNB3 825TT genotype than in subjects with 825CC genotype, but not significantly different with 825CT genotype. Conclusion A new rapid method for the detection of GNB3 825 site SNP has been successfully established. There existed significant correlation between GNB3 825TT genotype and obesity.

Hyperuricemia and metabolic syndrome were studied in 17 762 subjects of Tianjin Municipality from July 2007 to July 2009. The overall prevalence rate of hyperuricemia was 12. 16% (2160cases) , the rate in males was significantly higher than that in females (15. 71% vs. 6. 51% , P <0. 01).The overall prevalence rate of metabolic syndrome was 25. 56% (4540 cases) , the rate in males was also higher than that in females (28. 17% vs. 21. 40% , P <0. 01). Binary logistic regression analysis disclosed that females with high uric acid were twice likely to suffer from metabolic syndrome than males; and female ≤ 44 years with hyperuricemia had the highest odd ratio for metabolic syndrome.

Objective To identify the model parameters of surface Electromyography (sEMG) by comparison between simulated and recorded signals. Methods A physiological model of sEMG signal was established basing on several logical hypothetical conditions, such as motor unit action potentials (MUAP), motor unit recruitment and firing behavior caused by excitation, architecture of volume conductor and other simulated factors. According to the matched shapes between the simulated and recorded sEMG signals, a group of model parameters was obtained; according to the similar power spectrum variations of real sEMG signals, decreased muscle fiber conduction velocity (MFCV) was applied to simulate the sEMG signals of the fatigued muscle. Results The experimental results showed that the simulated superimposed MUAP shapes could be matched with the recorded MUAPs satisfactorily by adjusting some proper physiological parameters of the model. When the MFCV of each fiber was assumed to decrease, the mean and median frequency (MNF, MDF) of the simulated sEMG signals declined, and this phenomenon was very similar to that of the recorded sEMG signals and could be used to interpret the muscle fatigue process. Conclusion This model provides an effective approach to simulate real sEMG signals, and the simulated signals can also be used to help the analysis of recorded sEMG signals.

A total of 1507 children aged 7 ～ 12 years in Qinhuangdao were incruited to evaluate the diagnostic accuracy of different obesity indices in screening hypertension.Blood pressure and obesity indices were positively correlated(P＜0.01).Area under the receiver operating characteristic (ROC) curve were 0.744 ～ 0.835 in boys and 0.704 ～ 0.796 in girls (P＜0.01).Obesity indices could be used as parameters for detecting hypertension in children.