Objective To study the value of 5% glucose irrigation for dissection surface hemostasis in laparoscopic conservative treatment of tubal pregnancy. Methods Clinical parameters including numbers of failure in oviduct sparing, the postoperative intraperitoneal bleeding, persistent pregnancy, recurrent tubal pregnancy on the same side and normal uterine pregnancy were compared between the Experimental Group (glucose irrigation for dissection surface hemostasis; 43 cases) and the Control Group (unipolar electrocogulation hemostasis; 43 cases). Results We failed to reserve the oviduct in 10 patients in the Control Group (10/43, 23%) and in no patients in the Experimental Group (0/43) ( ? 2 =11 316, P =0 001). Recurrent tubal pregnancy on the same side was observed in 2 cases in the Control Group (2/34, 6%) and in no cases in the Experimental Group (0/34), without significant differences ( P =0 175). No postoperative intraperitoneal bleeding or persistent pregnancy was seen in both of the groups. Normal uterine pregnancy rates were 44 1% (15/34) in the Experimental Group and 40.0% (10/25) in the Control Group, without significant differences between the two groups ( ? 2 =0 100, P =0 752). Conclusions Use of 5% glucose irrigation is superior to electrocogulation for dissection surface hemostasis in the treatment of tubal pregnancy.

Objective Spermatogonial stem cells(SSCs)dissociated from 2～5 days postpartum mice were cultured on Sertoli cells feeders,to study the effect of Sertoli cells feeders on culture of mouse spermatogonial stem cells.Methods Specific markers CD9 of mouse SSCs cultured serum-free StemPro-34 SFM culture medium were identified by immunohistochemical assay.Result During the first week of culture,on Sertoli cells feeders or STO feeders,the biologica1 behaviors of spermatogonial stem cells showed no obvious difference.After a week of culture,compared with control,there were more number of spermatogonial stem cells remained when they were cultured for 60 days.These cells were expressed CD9 positive.In conclusion Sertoli cells can be used as feeders not only to promote survival but also renewal of spermatogonial stem cells.

Purpose:To explore the therapeutic effects of combining chemotherapy and TCM recipe “Xiao Zheng Fang” for advanced colorectal cancer.Methods:A prospective and comparable clinical study was made in 85 cases of advanced colorectal carcinoma seen over 6 years , Who were not suitable for radical operation or had metastases after operation. Survival time,life quality, objective effect and blood flow rate indexes were studied and analysed. Results:Integrated therapy of tranditional Chinese and Western medicine is superior to chemotherapy alone in life quality ( P

Impotence is one of the more common and serious symptom type of male sexual disturbance.It demonstrates by domestic sampling investigation that about more than 10% of male adult have impotence,and the incidence of impotence goes higher with the growing of age.Warming and invigorating kidney-yang has been the main therapy method for treating impotence.Through years of clinic practices,the treatment for impotence from liver has achieved satisfied effects.

Hospital Restructuring ; Hospitals ; Occupational Health Services

Objective To evaluate the clinical application of computer-aided electromagnetic imaging navigation in nasal endoscopic surgery.Methods Twenty-two cases of nasal endoscopic surgery with intraoperative imaging navigation were retrospectively reviewed,including 16 cases of sinusitis with or without polyp;5 cases of nasal inverting papilloma; 1 case of maxillary capillary hemangioma.All cases were operated with computer-aided electromagentic imaging navigation and nasal endoscope.Results The preoperative preparing time would take 4-10 minutes.In 22 cases,the localization accuracy between 3-D image landmarks of navigation system and actual anatomical landmarks was less than 1 mm.The optic nerve and other anatomical landmarks could be orientated accurately in intraoperative procedures.No complication occurred.Conclusions Nasal endoscope combined with computer-aided electromagnetic imaging navigation provides accurate anatomical localization of nasal cavity,sinuses and anterior skull base.It could improve the effectiveness and decrease surgical complications,especially in complicated cases.

Objective To investigate the mRNA expression of survivin, livin and XIAP gene in peripheral blood of patients with gastric cancer and its relationship with clinico-pathological features.Methods This study included 50 patients with gastric cancer and 20 healthy donors. The expression of survivin, livin and XIAP gene was detected by reverse transcription-quantitative polymerase chain reaction (RT-QPCR) using a molecular beacon probe, while recombination plasmid containing the sequence of survivin, livin and XIAP was standard. The relationship between copies of survivin, livin and XIAP gene expression in peripheral blood with gastric cancer and clinical data was analyzed. Results A linear standard curve was obtained between 103 ～ 1010 copies. The copies of survivin, livin and XIAP mRNA in peripheral blood of patients with gastric cancer did not correlate with gender, age, and histological types ( P ＞ 0.05). There were positive relationships between copies of survivin, livin and XIAP gene with lymph node metastasis and TNM stage (P ＜0.05 ). The expression of survivine, livin and XIAP was all negative in peripheral blood of healthy people. 52% (17/33) of patients suffered from recurrence or metastasis who had positive expression of survivin and/or livin and/or XIAP mRNA, while it was 18% (3/17)among the negative survivin and/or livin and/or XIAP mRNA caces ( P ＜ 0.05). Conclusions The expression of survivin, livin and XIAP mRNA can be used to detecte micro-metastasis in peripheral blood circulation of gastric cancer.

Osteoclasts are derived from pluripotent stem cells in bone marrow and spleen.They play a critical role in inflammation-induced bone loss and joint destruction because in the absence of them,bone de- struction does not occur even when inflammation exists.Synovioblasts in an inflamed joint can secrete numerous inflammatory factors,including tumor necrosis factor alpha(TNF-?)and interleukin-1(IL-1)which not only induce inflammatory reactions but also elevate osteoclast formation and function indirectly or directly through promoting RANKL expression.In this wdy the inflammatory reactions are associated with bone loss and destruction. In this article,we focus on the recent progress in study of TNF-?,IL-1 and osteoclast-target therapies in management of osteoclast-mediated inflammatory bone loss.TNF-?promotes differentiation of osteoclast precursor cells in the peripheral blood and spleen,which causes a marked increase in mature osteoclasts in a diseased joint.However, IL-I supports osteoblast survival and regulates the recombination of osteoclast cytoskeleton,which further stimulates bone resorption.Since osteoclast-target therapies may inhibit osteoclast formation and function,they are becoming more and more important for inflammation-induced bone loss and joint destruction.

Objective:To evaluate the effect of different shoulder width on the compressive strength of full-contour zirconia crowns.Methods:A computer aided design and computer aided manufacturing(CAD/CAM) system was used to prepare 24 full-contour zirconia crowns of the mandible first molar.1 mm occlusal thickness crowns with 4 shoulder width of knife-edge(group A),0.3 mm(group B),0.5 mm(group C) and 1.0 mm(group D) (n =6).The Co-Cr alloy PFM crowns made by the die of group C were used as the controls (n =6).All the full-contour zirconia crowns were bonded to the corresponding die using 3 M glass ionomer cement (GIC) and the compressive strength were measured by an universal testing machine.Based on the statistical analysis,crowns with the shoulder width of 0.5 mm were treated by the thermocycling test under cycling of 10000 times(group C1),20 000 times(group C2) and 30000 times (group C3) respectively(n =6).One-way ANOVA method was used for the statistical analysis.Results:The mean compressive strength(N) of group A,B,C,D and E was 3855.00 ± 305.47,2731.70 ± 261.80,3698.30 ± 276.87,3841.70 ± 544.88 and 2992.17 ± 168.41 respectively.The strength of group B and E were lower than that of group A,C and D(P ＜ 0.05),B vs E P ＞0.05.The compressive strength(N) of group C1,C2 and C3 was 3220.00 ± 504.38 (group C1),3148.33 ± 425.60(C2) and 2992.17 ±168.41 (C3) respectively (P＞0.05).Conclusion:For the full-contour zirconia crowns,both the knife-edge shoulder and the shoulder wider than 0.3 mm can meet the thermoclinical needs.The cycling times used in this study have no influences on the compressive strength.