Objective To clone human neural RNA binding protein HuC cDNA,express and purify the recombinant human HuC(Hu antigen C)protein in E.coli.Method Human HuC cDNA was cloned by RT-PCR.HuC cDNA was inserted into the prokaryotic expression vector pGEX-4T-3.The recombinant protein HuC was expressed in E.coli BL-21,and purified by the GST Sepharose 4B affinity column.Results The(62 ku) recombinant GST-HuC fusion protein was obtained.Conclusion The recombinant human HuC protein was successfully prokaryotic expressed and purified.

Humans ; Infant, Newborn ; Intermittent Positive-Pressure Ventilation

Objective: To study the clinical role of the variation of serum matrix metalloproteinase-8 (MMP-8) concentration in patients with acute coronary syndrome (ACS). Methods: ELISA method was adopted to detect serum MMP-8 concentration and to observe concentration's differences and features among 80 selected ACS cases (43 acute myocardial infarction and 37 unstable angina pectoris), 43 stable angina pectoris (SAP) cases and 37 control cases. And meanwhile the atherosclerosis risk factors of each case, such as age, sex, hypertension, body mass index, smoking, family history, diabetes, and hyperlipidemia were collected and analyzed as a whole. Results: First, serum MMP-8 concentration reached the highest point in ACS, and there was significant difference between SAP and control groups (P<0.01). Second, serum MMP-8 in AMI was much higher than that in UAP with significant difference (P<0.01). There was no difference between UAP and SAP groups (P>0.05). Third, Logistic regression analysis revealed that serum MMP-8 concentration might be the indicator of ACS (B=4.493, P=0.000), particularly, that of AMI (B=9.961, P=0.000). Fourth, linear correlation and linear regression analysis found that only neutrophil was likely to influence serum MMP-8 concentration (r=0.274, P=0.001). Fifth, in the diagnosis of ACS, the area under ROC curve of MMP-8 was 0.785, the sensitivity and specificity were 68.6% and 76.5%, respectively. Conclusion: 1 Serum MMP-8 concentration has close relationship with the occurrence of ACS, particularly with AMI; 2 Serum MMP-8 concentration may be one of the predicting indicators of ACS and particularly of AMI; 3 Neutrophil may be correlated with serum MMP-8 concentration; 4 MMP-8 is of somewhat valuable in diagnosing ACS.

Objective: To study the clinical role of the variation of serum matrix metalloproteinase-8 (MMP-8) concentration in patients with acute coronary syndrome (ACS). Methods: ELISA method was adopted to detect serum MMP-8 concentration and to observe concentration's differences and features among 80 selected ACS cases (43 acute myocardial infarction and 37 unstable angina pectoris), 43 stable angina pectoris (SAP) cases and 37 control cases. And meanwhile the atherosclerosis risk factors of each case, such as age, sex, hypertension, body mass index, smoking, family history, diabetes, and hyperlipidemia were collected and analyzed as a whole. Results: First, serum MMP-8 concentration reached the highest point in ACS, and there was significant difference between SAP and control groups (P<0.01). Second, serum MMP-8 in AMI was much higher than that in UAP with significant difference (P<0.01). There was no difference between UAP and SAP groups (P>0.05). Third, Logistic regression analysis revealed that serum MMP-8 concentration might be the indicator of ACS (B=4.493, P=0.000), particularly, that of AMI (B=9.961, P=0.000). Fourth, linear correlation and linear regression analysis found that only neutrophil was likely to influence serum MMP-8 concentration (r=0.274, P=0.001). Fifth, in the diagnosis of ACS, the area under ROC curve of MMP-8 was 0.785, the sensitivity and specificity were 68.6% and 76.5%, respectively. Conclusion: 1 Serum MMP-8 concentration has close relationship with the occurrence of ACS, particularly with AMI; 2 Serum MMP-8 concentration may be one of the predicting indicators of ACS and particularly of AMI; 3 Neutrophil may be correlated with serum MMP-8 concentration; 4 MMP-8 is of somewhat valuable in diagnosing ACS.

In this article,the authors introduced the functions of different catalogue of Traditional Chinese Medicine,together with the development of the past and present condition of catalogue.The authors also presented the opinion on how to work best with it.

Objective To evaluate the efficacy and safety of interventional occlsu ion operait no by analyizn g thes urgical data of 23 csa es of infants with patent ductusa rteriosus complicated with middle and severe pulmo an ry arterial hypertension.Methods Teh 23 cases of infants wiht patne t ductus arteriosus com-plicated with middlea nd severe pulmonary arterial hypertensionw ere collected in the hospital from January 2011 to December2014 .These infatn s rce eived transcateh ter occlusion with intravenuo s anesthesia after the preoperative examination.The operation procse s included:right ventriculography and pulmonary atr ery pressure tested,then lateral angiogar phy of descending aorta was performed to observe the type and size of patent ud ctus atr eriosus and measure ascending aorta,descending aortic pressure,and recorded the pressure re-spectively.1 ml blood sample of ascending aorta,pulmonary artery and inef rior vena vein respectively was used for gas analysis.All these data was used to calculate pulmonary vascular resistance.After tried to plug-ging effectiveyl we can release the occluder.In the postoperative 24 h,1 month,3 months,the infants should be measured with Doppler echocardiography,chest X ray and electrocardiogram examination.Results The clinical symptoms disappeared and the short-term follow-up was not associated with the complications of interventional therapy.Th e comparison of the pressure changes before and after the operation were performed as following, aortic per ssure decreased [ preoperation ( 68.3 ±17.5 )/( 21.4 ±3.7 ) mmHg, postoperation (52.4 ±8.7)/(15.6 ±3.5) mmHg,1 mmHg=0.133 kPa],ascending aorta pressure increased(preoperation (83.5 ±5.9)/(51.3 ±3.6) mmHg,postoperation(88.2 ±5.1)/(52.4 ±2.7) mmHg),and descending aorta pressure increased ( preoperation ( 81.4 ±3.3 )/( 48.2 ±2.7 ) mmHg, postoperation ( 86.5 ±4.7 )/(51.5 ±3.2) mmHg), the differences were statistically significant before and after surgery ( t =5.455/3.945 ,P<0.01;t=-2.696/-1.193 , P<0.05; t=-4.167/-3.745 , P<0.01 ) .Conclusion Under conditions of mastering the appropriate operation time and strengthening the management of the perioperative management,transcatheter measurement is safe and effective for infants with patent ductus arteriosus compli-cated with middle and severe pulmonary arterial hypertension.

Based on the analysis on the most common type of scientific research and clinical application of stem cells, including embryonic stem cells , hematopoietic stem cells and mesenchymal stem cells as the breakthrough point, discusses common phenomenon and problems of ethics , scientific research and clinical study the moral life science century new topic, hope scientists, physicians, ethicists, caused by the administrative departments for pub-lic health thought , promote stem cells healthy and steady development .

Blood Cell Count ; Child ; Fever ; Histiocytosis, Non-Langerhans-Cell ; diagnosis ; Humans ; Male ; Prognosis

Objective To study the optimum extration process of polysaccharides from Meretrix meretrix Linnaeus.A spectrophotometry method for content determination of polysaccharides from Meretrix meretrix Linnaeus was established with Phenol-sulfuric acid coloration.Methods The samples were extracted by ultrasonic wave,dissolved in 50mL,4mol?L~(-1) HCl and heated to hydrolyze by acid.The contents of polysaccharides from Meretrix meretrix Linnaeus were determined by spectrophotometry at 490nm.Results Extracted for thirty minutes by ultrasonic wave,two times,and acidic hydrolysis were the optimum technology.The calibration curve was linear over the range of 4.848～24.242mg?L~(-1).The regression equation was C=28.69A+0.229(r=0.9992).The average recovery rate was 100.67% and RSD was 2.83%(n=6).Conclusion The method was proved to be simple,accurate and reliable,and could be used to extract and determine the polysaccharides from Meretrix meretrix Linnaeus.